Patents Assigned to SPIBER INC.
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Publication number: 20190352349Abstract: The present invention relates to a protein composition formed of dry matter of a dispersion in which a protein is dispersed in a stabilizer solution, in which the stabilizer includes at least one type selected from the group consisting of a catechol-based stabilizer, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, 2,6-di-tert-butyl-p-cresol, 2-tert-butyl-4-methoxyphenol, and 6,6?-di-tert-butyl-4,4?-butylidenedi-m-cresol.Type: ApplicationFiled: November 29, 2017Publication date: November 21, 2019Applicants: Spiber Inc., Kojima Industries CorporationInventors: Toshiaki Taniike, Anh Thi Ngoc Dao, Koyuru Nakayama, Junichi Shimokata, Kengo Takeuchi, Abhijit Ravikiran Muley, Sunita Darbe
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Publication number: 20190233481Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence locally containing a region with locally high hydropathy index equivalent to an amino acid sequence in which one or a plurality of amino acid residues in REP are substituted with amino acid residues with a high hydropathy index and/or one or a plurality of amino acid residues with a high hydropathy index are inserted into REP, as compared to naturally occurring fibroin. In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300, A plurality of (A)n motifs and the like may be the same amino acid sequence or different amino acid sequences.Type: ApplicationFiled: June 23, 2017Publication date: August 1, 2019Applicants: Spiber Inc., KOJIMA INDUSTRIES CORPORATIONInventors: Keisuke MORITA, Koichi KOTAKA
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Publication number: 20190225646Abstract: Disclosed is a method for purifying a recombinant protein of interest from a recombinant cell expressing intracellularly the recombinant protein as an insoluble matter, comprising treating the recombinant cell under conditions where a protein derived from a host cell dissolves in a first aprotic polar solvent to which an inorganic salt is added or not but the recombinant protein does not dissolve, removing a first soluble fraction, and then treating the resulting first insoluble fraction under conditions where the recombinant protein dissolves in a second aprotic polar solvent to which an inorganic salt is added.Type: ApplicationFiled: October 3, 2017Publication date: July 25, 2019Applicant: Spiber Inc.Inventor: Toshimasa Homma
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Publication number: 20190194403Abstract: The present invention provides a method for producing a molded article including a step 1 of adding water to a composition including a structural protein, a step 2 of molding a water-containing composition obtained in the step 1, and a step 3 of drying the molded composition obtained in the step 2 to obtain the molded article.Type: ApplicationFiled: September 1, 2017Publication date: June 27, 2019Applicants: Spiber Inc., Tekunohama Co., Ltd.Inventors: Shinji Hirai, Yukimasa Ubukata, Ayumi Abe, Koichi Kotaka, Yuki Nakayama, Junichi Noba
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Publication number: 20190194710Abstract: The invention relates to a method for production of a recombinant protein using recombinant cells that express the recombinant protein under the control of an inducible promoter, the method including continuous culturing with addition of fresh culture medium to a portion of the culture solution in which the recombinant cells have been grown, wherein the recombinant cells whose expression of the recombinant protein has been induced are not reused.Type: ApplicationFiled: August 2, 2017Publication date: June 27, 2019Applicant: Spiber Inc.Inventors: Kenji Kurachi, Mihoko Kinoshita
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Patent number: 10329332Abstract: A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.Type: GrantFiled: December 17, 2013Date of Patent: June 25, 2019Assignee: SPIBER INC.Inventors: Kaori Sekiyama, Mizuki Ishikawa, Shinya Murata
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Publication number: 20190186050Abstract: Provided are a composite molding composition such as a composite film and a composite fiber that have properties such as an improved tensile strength, strain and toughness as a result of blending a peptide or polyamino acid having a ?-sheet structure with a natural or artificially modified fibroin-derived protein; a method for producing such composite molding composition; and a method for improving the physical properties of a composite molding composition containing a fibroin-derived protein.Type: ApplicationFiled: July 24, 2017Publication date: June 20, 2019Applicants: RIKEN, SPIBER INC.Inventors: Keiji NUMATA, Kousuke TSUCHIYA, Junichi SUGAHARA, Takaoki ISHII, Hongfang CHI
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Publication number: 20190177363Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.Type: ApplicationFiled: August 10, 2017Publication date: June 13, 2019Applicant: Spiber Inc.Inventors: Toshiaki Osawa, Yuya Sato, Keisuke Morita
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Publication number: 20190135881Abstract: Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of glycine residues equivalent to an amino acid sequence in which, at least, one or a plurality of the glycine residues in REP is substituted with another amino acid residue, as compared to naturally occurring fibroin: [In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.Type: ApplicationFiled: April 28, 2017Publication date: May 9, 2019Applicants: Spiber Inc., Kojima Industries CorporationInventors: Keisuke Morita, Junichi Sugahara
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Publication number: 20190135880Abstract: Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of (A)n motif equivalent to an amino acid sequence in which, at least, one or a plurality of the (A)n motifs is deleted, as compared to naturally occurring fibroin. [In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.Type: ApplicationFiled: April 28, 2017Publication date: May 9, 2019Applicants: Spiber Inc., Kojima Industries CorporationInventors: Keisuke Morita, Hiroyuki Nakamura
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Publication number: 20190040109Abstract: Provided is a molded article of a composition comprising a polypeptide, wherein the polypeptide is at least one kind selected from the group consisting of natural spider silk protein and polypeptides derived from natural spider silk protein.Type: ApplicationFiled: September 8, 2016Publication date: February 7, 2019Applicants: Spiber Inc., Kojima Industries Corporation, Tekunohama Co., Ltd.Inventors: Junichi Sugahara, Kaori Sekiyama, Ayumi Abe, Junichi Shimokata, Junichi Noba, Shinji Hirai
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Publication number: 20190031842Abstract: The present invention provides, in one aspect, a method for producing a molded article, the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 80% or more to obtain the molded article.Type: ApplicationFiled: January 27, 2017Publication date: January 31, 2019Applicants: Riken, Spiber Inc., Kojima Industries CorporationInventors: Keiji Numata, Kana Ishida, Hironori Yamamoto
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Publication number: 20190031843Abstract: The present invention provides, in one aspect, a method for producing a molded article, the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 90% or more to obtain the molded article.Type: ApplicationFiled: January 27, 2017Publication date: January 31, 2019Applicants: Riken, Spiber Inc.Inventors: Keiji Numata, Kana Ishida, Hironori Yamamoto
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Publication number: 20190002644Abstract: The present invention relates to a method for producing a polymer aggregate, the method including: a step for bringing a polymer solution containing a polymer substance and cations for gel formation into contact with an anion solution containing anions for gel formation to form a gel at an interface between the polymer solution and the anion solution; and a step for causing the polymer substance to aggregate inside the gel.Type: ApplicationFiled: December 21, 2016Publication date: January 3, 2019Applicant: Spiber Inc.Inventors: Yoshinori OHTA, Hiroaki SUZUMURA
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Publication number: 20180355120Abstract: Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.Type: ApplicationFiled: November 29, 2016Publication date: December 13, 2018Applicants: Spiber Inc., Kojima Industries Corporation, Tekunohama Co., Ltd.Inventors: Koichi Kotaka, Takaoki Ishii, Junichi Sugahara, Ryota Sato, Junichi Noba
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Publication number: 20180265555Abstract: A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method of culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein accumulation of an organic acid at the time of inducing the expression is reduced, and wherein the gene is expressed under control of a tryptophan promoter.Type: ApplicationFiled: May 25, 2018Publication date: September 20, 2018Applicants: AJINOMOTO CO., INC., SPIBER INC.Inventors: Kentaro Nakase, Yoshinori Takashima, Takeshi Nagahiko
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Publication number: 20180251501Abstract: Provided is a molded article of a composition comprising a polypeptide which has more than 4.3 GPa of a bending elastic modulus and transparency.Type: ApplicationFiled: September 8, 2016Publication date: September 6, 2018Applicants: Spiber Inc., Kojima Industries Corporation, Tekunohama Co., Ltd.Inventors: Junichi Sugahara, Kaori Sekiyama, Ayumi Abe, Junichi Shimokata, Junichi Noba, Shinji Hirai
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Patent number: 10065997Abstract: A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.Type: GrantFiled: April 18, 2014Date of Patent: September 4, 2018Assignee: SPIBER INC.Inventors: Toshiaki Osawa, Keisuke Morita
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Patent number: 9968682Abstract: A polypeptide hydrogel of the present invention is a hydrogel of a polypeptide derived from spider silk proteins, and has a moisture content of 85.0 to 99.9 mass % based on 100 mass % of the polypeptide hydrogel. A method for producing the hydrogel includes: a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide; and a substitution step in which the solution produced in the solution production step is substituted with a water-soluble solvent. Thus, the present invention provides a polypeptide hydrogel having excellent water retentivity and a polypeptide hydrogel suitable for application to a living body.Type: GrantFiled: April 18, 2014Date of Patent: May 15, 2018Assignee: SPIBER INC.Inventors: Toshiaki Osawa, Keisuke Morita
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Patent number: 9926348Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the accumulation of an organic acid at the time of inducing the expression is reduced.Type: GrantFiled: November 21, 2016Date of Patent: March 27, 2018Assignees: Ajinomoto Co., Inc., Spiber Inc.Inventor: Kentaro Nakase