Abstract: Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided.

Abstract: Method of making a low resistivity electrical connection between an electrical conductor and an iron pnictide superconductor involves connecting the electrical conductor and superconductor using a tin or tin-based material therebetween, such as using a tin or tin-based solder. The superconductor can be based on doped AFe2As2, where A can be Ca, Sr, Ba, Eu or combinations thereof for purposes of illustration only.

Abstract: An impeller is provided that may be used in compressors or turbines. In another aspect of the present invention, a fiber or a bundle of fibers is woven to form at least two blades of an impeller. Yet another aspect of the present invention employs a peripheral component woven around impeller blades. An additional conductive fiber or bundle of fibers is woven into the impeller in a further aspect of the present invention. Moreover, an aspect of the present invention provides a chilling system that includes at least one compressor, at least one wave rotor, and a refrigerant.

Abstract: Method and apparatus for performing spectroscopy, include the combining of first and second light beams to form a reference beam, focusing the first and second light beams and the reference beam onto a sample, receiving a reflected light beam from the sample at a monochromator, and viewing a predetermined wavelength band of the reflected light beam from the monochromator. Portions of the first and second light beams, which may be visible and IR forms of electromagnetic energy, are heterodyned through a crystal. A monochromator receives a reflection of the reference beam from the sample, and Fourier transformation is performed on the output of the monochromator. The first and second beams of electromagnetic energy can be split to form first and second component beams and the reference beam, all of which are propagated to the sample.

Abstract: Malignant tumors that are intrinsically resistant to conventional therapies are significant therapeutic challenges. An embodiment of the present invention provides an oncolytic virus capable of killing target cells, such as a tumor cells. In various embodiments presented herein, the oncolytic virus is armed or encodes a therapeutic polypeptide. In at least one embodiment, a recombinant oncolytic virus has been generated that can specifically replicate in cancer cells leading to their destruction and at the same time secrete robust amounts of a therapeutic polypeptide. Compositions and methods disclosed herein have broad therapeutic applicability.

Abstract: Disclosed herein is a set of E. coli single-protein production (SPP) technologies with protein NMR (SPP-NMR) for (i) using isotope-enriched membrane proteins produced with the SPP system in screening detergent conditions suitable for purification and/or three-dimensional structure analysis without the requirement for protein purification, (ii) producing 2H, 13C, 15N enriched proteins suitable for high throughput and membrane protein NMR studies, and (iii) labeling with 13C—15N specific peptide bonds in proteins (referred to herein as SPP-PBL).

Abstract: Improved methods of native chemical ligation are provided. The methods involve reacting a thioacid (e.g. a peptide thioacid) with an aziridinyl compound (e.g. an aziridinyl peptide) under mild conditions without the use of protecting groups, and without requiring that a cysteine residue be present in the ligation product. Initial coupling of the thioacid and the aziridinyl compound yields a ligation product which contains an aziridinyl ring. Subsequent opening of the aziridinyl ring (e.g. via a nucleophilic attack) produces a linearized and modified ligation product.

Abstract: Reliable, fast and inexpensive breath gas detector systems for medical diagnostics, including personal, handheld monitoring devices for a variety of diseases and conditions, including, for example, asthma, diabetes, blood cholesterol, and lung cancer. A sensor device (100) for detecting gases includes a sensing element (109) having an electrical resistance that changes in the presence of a target gas; a readout circuit, electrically coupled to the sensing element due to the presence of the target gas and converts the measurement to a digital signal; and a feedback loop (203) from a digital unit (205) to the readout circuit to compensate for variations in a baseline resistance of the sensing element.

Abstract: A composition comprises a substantially dry mixture of substantially dry corn gluten meal and substantially dry solid acid, wherein the amount of the substantially dry solid acid is effective to enhance the pre-emergence herbicidal activity of the substantially dry corn gluten meal. A method for selectively inhibiting the growth of undesirable plants comprises prior to the emergence of the undesirable plants, applying a substantially dry corn gluten meal and a substantially dry solid acid to a plot of soil so that the amount of the substantially dry solid acid applied to the plot of soil is effective to enhance the pre-emergence herbicidal activity of the substantially dry corn gluten meal, wherein the substantially dry corn gluten meal and the substantially dry solid acid are applied in an amount effective to inhibit the growth of the undesirable plants.

Abstract: An electricity generating shock absorber includes a coil assembly having a length of electrically conducting material wrapped around an outside perimeter, and along a length, of a hollow tube formed of electrically resistant material; a magnet unit formed of at least one annular axial magnet; a central shaft having a magnetic reluctance on which a plurality of the magnet units are mounted, the central shaft dimensioned for insertion through a central opening of the at least one annular axial magnet, the central shaft combined with the plurality of magnet units forming a magnet assembly dimensioned to slideably insert into a central cavity of the hollow tube; and a cylindrical shell having a first end attached to a terminal end of the magnet assembly, the cylindrical shell extending a length of the magnet assembly, the cylindrical shell having an inner diameter sized to slideably accommodate an outside diameter of the coil assembly.

Abstract: Disclosed is a piezoelectric foam formed of elastically anisotropic materials. The piezoelectric foam is defined with a unit cell having a relative density and volume fraction, and deformation specified by subjecting the unit cell to controlled mechanical and electrical loading conditions. Resultant stress and electric displacements field components are measured by capturing a homogeneous coupled response of the unit cell and by computing piezoelectric material constants using the captured homogeneous coupled response, to identify asymmetric and symmetric F1, F2 and F3 type piezoelectric foam structures.

Abstract: A high-throughput screening method for identifying new anti-fungal compounds, including: growing an adenine-requiring mutant fungus under conditions to induce bioaccumulation of P-ribosylaminoimidazole; treating the mutant fungus with chemical compounds; and determining if the chemical compounds cause white discoloration of the mutant fungus. A method to determine the effective concentration of an anti-fungal. A method to detect chemical disruption of the fungal vacuole of a fungus.

Abstract: The present invention is directed to a hierarchical structure characterized by ultrahigh surface area comprising: a solid substrate; an intermediate layer; and at least one plurality of nanoscale attachments that are strongly bonded to the intermediate layer. Also disclosed is a method of fabricating a hierarchical structure comprising: selecting and preparing a parent substrate, wherein the preparing may optionally include cleaning or activation; modifying the substrate surface to form an intermediate layer; attaching at least one plurality of nanoscale attachments, wherein the nanoscale attachments are selected from nanotubes, nanoparticles, or combinations thereof, onto the intermediate layer; optionally attaching a second plurality of nanoscale attachments, wherein the nanoscale attachments are selected from nanotubes, nanoparticles, or combinations thereof, onto the first plurality of nanoscale attachments and intermediate layer.

Abstract: The computer-implemented system to facilitate creation of educational information employs a networked computer system that stores at least one resource in association with a first electronic file, storing metadata information about usage of the resource. A resource assembly tool implemented by a computer is programmed to access the networked computer system to display information to an instructor about at least one resource, including the metadata information. This aids the instructor in selecting resources for inclusion in educational information being created. The resource assembly tool is configured to assemble the educational information to include resources selected for inclusion being created for dissemination to learners via said networked computer system. The networked computer system is further configured to capture information about usage of the resource and to update the stored metadata information to reflect said captured information.

Abstract: A carbothermic reduction method is provided for reducing a La-, Ce-, MM-, and/or Y-containing oxide in the presence of carbon and a source of a reactant element comprising Si, Ge, Sn, Pb, As, Sb, Bi, and/or P to form an intermediate alloy material including a majority of La, Ce, MM, and/or Y and a minor amount of the reactant element. The intermediate material is useful as a master alloy for in making negative electrode materials for a metal hydride battery, as hydrogen storage alloys, as master alloy additive for addition to a melt of commercial Mg and Al alloys, steels, cast irons, and superalloys; or in reducing Sm2O3 to Sm metal for use in Sm—Co permanent magnets.

Abstract: The present disclosure relates to the development and use of Closterovirus-based vectors for the delivery of nucleotides to plants. Specifically, the present disclosure provides viral vectors based on Grapevine leafroll-associated virus-2 for the delivery and expression of genes in plants, particularly grape plants. Methods of making and using these vectors, as well as the plants transformed by these vectors, are also contemplated.

Abstract: A fuel cell comprising an anode electrode, a cathode electrode and a reference electrode electronically connected to each other; a first biocatalyst comprising a consolidated bioprocessing organism (e.g., a cellulomonad or clostridium or related strains, such as Cellulomonas uda (C. uda), C. lentocellum, A. cellolulyticus, C. cellobioparum, alcohol-tolerant C. cellobioparum, alcohol-tolerant C. uda, Clostridium cellobioparum (C. cellobioparum) and combinations thereof) capable of fermenting biomass (e.g., cellulosic biomass or glycerin-containing biomass) to produce a fermentation byproduct; and a second biocatalyst comprising an electricigen (e.g., Geobacter sulfurreducens) capable of transferring substantially all the electrons in the fermentation byproduct (e.g., hydrogen, one or more organic acids, or a combination thereof) to the anode electrode to produce electricity is disclosed. Systems and methods related thereto are also disclosing a consolidated bioprocessing organism.

Abstract: Mutant fibroblast growth factor (FGF) proteins having a polypeptide sequence with a high sequence identity to proteins encoded by members of the Fgf-1 subfamily of genes from a mammalian species, such as human, and with a specific amino acid substitution of an alanine at a position corresponding to amino acid position 66 of human FGF-1 with a cysteine and/or a specific amino acid substitution of a phenylalanine at a position corresponding to amino acid position 132 of human FGF-1 with a tryptophan (based on the 140 amino acid numbering scheme of human FGF-1) are provided. Other amino acid mutations or substitutions may be combined. Polynucleotide sequences encoding the mutant FGF proteins and host cells containing such polynucleotide sequences are provided. Methods of administering a mutant FGF protein to an individual to treat an ischemic condition or disease or a wound or tissue injury are also provided.

Abstract: The present inventions relate to accurately identifying a subset of patients within a larger group with malarial parasitemia. In particular, the present inventions provide compositions and methods comprising a malarial protein, histidine rich pro-tein-2 (HRP-2) for determining the general severity of a malarial infection in patients. Specifically, the inventions provide a rapid test comprising a read-out for HRP-2 levels in bodily fluids for determining whether a comatose patient's disease is a result of malaria as opposed to coma of another cause with incidental parasitemia. Specifically, in one preferred embodiment, a rapid test is contemplated as a quantitative rapid test dipstick. Further, these inventions relate to predictive tests for patients at risk for progression of relatively mild malaria disease to the more life-threatening cerebral malaria in addition to determining the etiology of malaria infections in comatose patients.

Abstract: An apparatus and method are disclosed for using a thermally active device as a flow meter. The flow meter may have an extremely low mass, rapid response time, and use minimal energy. The flow meter may be located near a flow-side surface of a conduit wall, flush with the surface of a wall, or within a boundary layer of a flow in a conduit. In these locations, the device may present virtually no obstruction to the flow. In certain embodiments, the device may use a resistance temperature device (RTD) heated by a known current, and then tested for resistance at a comparatively much lower (nominally zero) value. A flow rate may be calculated as a function of temperature measurements taken at different steady-state conditions. Flow rates may be so measured at any desired frequency, including very infrequently, such as seconds, minutes, or days apart.