Abstract: Provided are bifunctional molecules that include a first moiety that specifically binds a cell surface molecule or extracellular molecule, and a second moiety that specifically binds a lysosomal targeting molecule. The bifunctional molecules find use, e.g., for targeted degradation of cell surface and extracellular molecules (e.g., proteins) via the endosomal/lysosomal pathway. Also provided are compositions and kits that include the bifunctional molecules, as well as methods of using the bifunctional molecules. Methods of making bifunctional molecules are also provided.

Abstract: A stacked three-dimensional (3D) metasurface includes a first layer with a first kernel configured to apply a first convolution on a first frequency of an incident polarized light, and a second layer with a second kernel different than the first kernel. The second kernel is configured to apply a second convolution different than the first convolution on a second frequency of the incident polarized light and the second frequency is different than the first frequency such that the stack 3D metasurface provides at least two independent convolutions on the incident polarized light.

Abstract: Provided herein are materials and methods useful for facilitating transgene recombination. The present disclosure relates to, for example, techniques for manipulating recombination frequencies and generating organisms that contain multiple transgenic elements docking at the same locus on a single chromosome. The time consumed by the entire recombination process is proportional to the logarithm of the number of transgenes to be recombined.

Abstract: The present disclosure relates to a lithium secondary battery anode provided with an interfacial layer including a coordination compound and a method of manufacturing the same. The anode may include an anode current collector layer, an anode material layer disposed on the anode current collector layer and including lithium metal, and an interfacial layer disposed on the anode material layer and including a coordination compound.

Abstract: A device may obtain triaxial accelerometer training data for a plurality of subjects. A device may define a number of feature vectors for each time interval and each subject of the triaxial accelerometer data. A device may cluster the feature vectors of the triaxial training data into a number of clusters to obtain a cluster assignment for each of the feature vectors of each subject. A device may fit a hidden Markov model to the to the triaxial accelerometer training data cluster assignments. A device may identify at least one state for the subjects based on the cluster assignments.

Abstract: A method for self-supervised learning is described. The method includes generating a plurality of augmented data from unlabeled image data. The method also includes generating a population augmentation graph for a class determined from the plurality of augmented data. The method further includes minimizing a contrastive loss based on a spectral decomposition of the population augmentation graph to learn representations of the unlabeled image data. The method also includes classifying the learned representations of the unlabeled image data to recover ground-truth labels of the unlabeled image data.

Abstract: Methods are provided for treating a subject with for an intracellular pathogen infection, by administering an agent that reduces the binding of CD47 on a infected cell to SIRP? on a host phagocytic cell, in an effective dose for increasing the phagocytosis of infected cells.

Abstract: Engineered proteins, polynucleotides encoding such proteins, and methods of use thereof are provided, which engineered proteins enhance the sensitivity of a cell to IL-2.

Abstract: Markers of acute myeloid leukemia stem cells (AMLSC) are identified. The markers are differentially expressed in comparison with normal counterpart cells, and are useful as diagnostic and therapeutic targets.

Abstract: Processes and materials to detect cancer, transplant rejection, or fetal genetic abnormalities from a biopsy are described. In some cases, nucleic acid molecules, such as cell-free nucleic acids, can be sequenced, and the sequencing result can be utilized to detect sequences indicative of a neoplasm, transplant rejection, or fetal genetic abnormality. Detection of somatic variants occurring in phase and/or insertions and deletions (indels) can indicate the presence of cancer, transplant rejection, or fetal genetic abnormalities in a diagnostic scan, and a clinical intervention can be performed.

Abstract: Embodiments of the invention are generally directed to lignin-modifying enzymes and systems and methods of their manufacture. In many embodiments, yeast strains, including S. cerevisiae, are used to produce and secrete lignin-modifying enzymes Further embodiments are directed to methods to screening peroxidase-producing yeast strains, including S. cerevisiae. Additional embodiments are directed to an expression vector or cassette encoding for a protein of interest and one or more proteins to allow for surface to display of the protein of interest.

Abstract: Systems and methods for generating ligand compound structures are provided. A trained computational framework can utilize an initial core ligand compound structure to generate a ligand compound structure by iteratively adding atomic structures. At each iterative step, the computational framework can select a location for adding an atomic structure and can further select which atomic structure is to be added the selected location.

Abstract: Systems and methods for seizure detection in accordance with embodiments of the invention are illustrated. One embodiment includes an automated seizure treatment system, including an electroencephalogram (EEG) device configured to record neural activity from a brain of a patient, a treatment device, a processor, and a memory, the memory containing a seizure detection application that configures the processor to obtain an EEG signal from the EEG device, calculate an inverse compression ratio based on the EEG signal, and when the inverse compression ratio is greater than a classification threshold, deliver treatment capable of stopping the seizure to the patient using the treatment device.

Abstract: A method is disclosed for generating pixel risk maps for diagnostic image reconstruction. The method produces uncertainty/variance maps by feeding into a trained encoder a short-scan image acquired from a medical imaging scan to generate latent code statistics including the mean ?y and variance ?y; selecting random samples based on the latent code statistics, z˜N(?y,?y2); feeding the random samples into a trained decoder to generate a pool of reconstructed images; and calculating, for each pixel of the pool of reconstructed images, the pixel mean and variance statistics across the pool of reconstructed images. The risk of each pixel may be calculated using the Stein's Unbiased Risk Estimator on the input density compensated data, that involves calculating the end-to-end divergence of the deep neural network.

Abstract: A stimulation system stimulates anatomical targets in a patient for treatment of dry eye. The system may include a controller and a microstimulator. The controller may be implemented externally to or internally within the microstimulator. The components of the controller and microstimulator may be implemented in a single unit or in separate devices. When implemented separately, the controller and microstimulator may communicate wirelessly or via a wired connection. The microstimulator may generate pulses from a controller signal and apply the signal via one or more electrodes to an anatomical target. The microstimulator may not have any intelligence or logic to shape or modify a signal. The microstimulator may be a passive device configured to generate a pulse based on a signal received from the controller. The microstimulator may shape or modify a signal. Waveforms having different frequency, amplitude and period characteristics may stimulate different anatomical targets in a patient.

Abstract: The present invention relates to a system and method for efficiently modifying the genome of cells to treat diseases via sequential homologous recombination using CRISPR/Cas-mediated genome editing with donor DNA delivered by two or more adeno-associated virus (AAV) vectors.

Abstract: Implantable devices and/or sensors can be wirelessly powered by controlling and propagating electromagnetic waves in a patient's tissue. Such implantable devices/sensors can be implanted at target locations in a patient, to stimulate areas such as the heart, brain, spinal cord, or muscle tissue, and/or to sense biological, physiological, chemical attributes of the blood, tissue, and other patient parameters. The propagating electromagnetic waves can be generated with sub-wavelength structures configured to manipulate evanescent fields outside of tissue to generate the propagating waves inside the tissue. Methods of use are also described.

Abstract: Markers of acute myeloid leukemia stem cells (AMLSC) are identified. The markers are differentially expressed in comparison with normal counterpart cells, and are useful as diagnostic and therapeutic targets.

Abstract: The present invention concerns the enhancement of the osteogenic potential of bone graft by ex vivo treatment with a Wnt polypeptide, such as a liposomal Wnt polypeptide.

Abstract: The present disclosure provides systems, compositions and methods for regulating expression of a target polynucleotide in a cell. The systems, compositions and methods comprise a chimeric receptor polypeptide comprising a G-protein coupled receptor (GPCR) or a fragment thereof, a chimeric adaptor polypeptide, at least one actuator moiety and a cleavage moiety.