Patents Assigned to The Institute of Physical & Chemical Research
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Patent number: 7531076Abstract: Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. it fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.Type: GrantFiled: January 6, 2006Date of Patent: May 12, 2009Assignees: The Institute of Physical and Chemical Research, Shimadzu Corporation, Japan Science and Technology AgencyInventors: Yoshihide Hayashizaki, Rintaro Yamamoto
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Publication number: 20070238158Abstract: A method for enhancing activity of enzyme at an elevated temperature which comprises adding a substance exhibiting chaperone function such as a saccharide to a reaction mixture containing the enzyme. The method can improve activity of enzymes more easily and more effectively and hence afford increased enzyme activity at an elevated temperature.Type: ApplicationFiled: March 29, 2007Publication date: October 11, 2007Applicant: The Institute of Physical and Chemical ResearchInventor: Yoshihide Hayashizaki
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Patent number: 7236298Abstract: An illumination apparatus for a microscope and an image processing apparatus using the illumination apparatus include a light source, a semi-transmissive mirror splitting a light beam from the light source into two beams of the first and second irradiation light, two excitation filters selecting the wavelengths of the first and second irradiation light, a semi-transmissive mirror synthesizing individual beams of the first and second irradiation light whose wavelengths are selected, into a single beam, a dichroic mirror directing a light beam synthesized by the semi-transmissive mirror toward a specimen and transmitting light from the specimen, an objective lens, cameras imaging fluorescent light from the specimen after being separated into fluorescent light excited by the first and second wavelengths, and an image processing section processing fluorescent images formed by imaging elements.Type: GrantFiled: September 13, 2006Date of Patent: June 26, 2007Assignees: The Institute of Physical & Chemical Research, Olympus CorporationInventors: Atsushi Miyawaki, Takashi Fukano, Yasushi Aono
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Patent number: 7195698Abstract: An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.Type: GrantFiled: December 13, 2002Date of Patent: March 27, 2007Assignees: The Institute of Physical and Chemical Research, Shimadzu Corporation, Japan Science and Technology CorporationInventors: Yoshihide Hayashizaki, Shin Nakamura, Makoto Hazama, Hideshi Fujiwake
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Publication number: 20070008615Abstract: An illumination apparatus for a microscope and an image processing apparatus using the illumination apparatus include a light source, a semi-transmissive mirror splitting a light beam from the light source into two beams of the first and second irradiation light, two excitation filters selecting the wavelengths of the first and second irradiation light, a semi-transmissive mirror synthesizing individual beams of the first and second irradiation light whose wavelengths are selected, into a single beam, a dichroic mirror directing a light beam synthesized by the semi-transmissive mirror toward a specimen and transmitting light from the specimen, an objective lens, cameras imaging fluorescent light from the specimen after being separated into fluorescent light excited by the first and second wavelengths, and an image processing section processing fluorescent images formed by imaging elements.Type: ApplicationFiled: September 13, 2006Publication date: January 11, 2007Applicants: THE INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH, OLYMPUS CORPORATIONInventors: Atsushi Miyawaki, Takashi Fukano, Yasushi Aono
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Patent number: 7126752Abstract: An illumination apparatus for a microscope and an image processing apparatus using the illumination apparatus include a light source, a semi-transmissive mirror splitting a light beam from the light source into two beams of the first and second irradiation light, two excitation filters selecting the wavelengths of the first and second irradiation light, a semi-transmissive mirror synthesizing individual beams of the first and second irradiation light whose wavelengths are selected, into a single beam, a dichroic mirror directing a light beam synthesized by the semi-transmissive mirror toward a specimen and transmitting light from the specimen, an objective lens, cameras imaging fluorescent light from the specimen after being separated into fluorescent light excited by the first and second wavelengths, and an image processing section processing fluorescent images formed by imaging elements.Type: GrantFiled: November 20, 2003Date of Patent: October 24, 2006Assignees: The Institute of Physical and Chemical Research, Olympus CorporationInventors: Atsushi Miyawaki, Takashi Fukano, Yasushi Aono
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Publication number: 20060113191Abstract: Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. it fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.Type: ApplicationFiled: January 6, 2006Publication date: June 1, 2006Applicants: The Institute of Physical and Chemical Research, Shimadzu Corporation, Japan Science and Technology CorporationInventors: Yoshihide Hayashizaki, Rintaro Yamamoto
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Patent number: 7014745Abstract: Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. It fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.Type: GrantFiled: March 31, 2003Date of Patent: March 21, 2006Assignees: The Institute of Physical and Chemical Research, Shimadzu Corporation, Japan Science and Technology CorporationInventors: Yoshihide Hayashizaki, Rintaro Yamamoto
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Patent number: 6921648Abstract: The present invention relates to neurogenesis inducing genes. In particular, the present invention provides neurogenesis inducing genes coding for Zic proteins, vectors containing such genes, host cells containing such vectors, proteins produced by such host cells, antibodies raised to such proteins, and therapeutic agents or agents for gene therapy for nervous diseases.Type: GrantFiled: September 16, 2002Date of Patent: July 26, 2005Assignee: The Institute of Physical and Chemical ResearchInventors: Katsuhiko Mikoshiba, Jun Aruga, Takeharu Nagai, Katsunori Nakata
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Patent number: 6867027Abstract: Disclosed are RNA polymerases consisting of a wild type RNA polymerase provided that at least one of amino acids in the wild type RNA polymerase has been modified to enhance its ability for incorporating 3?-deoxyribonucleotides and derivatives thereof in comparison with the corresponding wild type RNA polymerases. Specifically, disclosed are, for example, the RNA polymerases wherein at least one amino acid present in a nucleotide binding sites of the wild type RNA polymerases such as phenylalanine has been replaced with tyrosine. The RNA polymerases of the present invention are a RNA polymerase which exhibits little or no bias for incorporation between ribonucleotides and 3?-deoxyribonucleotide as well as among ribonucleotides having different base groups and among deoxyribonucleotides having. different base groups.Type: GrantFiled: July 6, 1998Date of Patent: March 15, 2005Assignees: The Institute of Physical and Chemical Research, Nippon Gene Co., Ltd., Nippon Genetech Co., Ltd.Inventors: Yoshihide Hayashizaki, Masanori Watahiki
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Patent number: 6839634Abstract: A molecule surrounding surface (20) is set so as to be reflected in the spatial dimension of a molecule. A molecule surrounding space surrounded by the molecule surrounding surface is divided into a plurality of component spaces (22), by which the reaction characteristic of the molecule is characterized. Probe points are provided on a frontier surrounding surface (5) on the molecule surrounding surface, and a space occupied rate is derived for each of the component spaces. Electrostatic energies are derived for each of the probe points on the frontier surrounding surface, and the sum of the electrostatic energies on the frontier surrounding surface is derived as a enelectrostatic factor of each of the component spaces. In addition, van der Waals energies are derived for each of the probe points, and the sum of the van der Waals energies on the frontier surrounding surface is derived as a steric factor of each of the component spaces.Type: GrantFiled: March 12, 1999Date of Patent: January 4, 2005Assignee: The Institute of Physical and Chemical ResearchInventors: Hiroko Satoh, Kimito Funatsu, Tadashi Nakata, Keiko Takano
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Patent number: 6783650Abstract: A capillary cassette is made up of two separated holders, an array of capillaries and a tube. The capillaries that make up the array are inserted into the first holder, extend through second holder having a detection window and then the capillary array is aligned and then bundled and inserted into a heat shrinkable tube which covers positions to which filler is applied, the tube is heated and shrunk to bundle them in an airtight manner and inserted into a mounting member.Type: GrantFiled: April 22, 2002Date of Patent: August 31, 2004Assignees: The Institute of Physical & Chemical Research, Shimadzu CorporationInventors: Yoshihide Hayashizaki, Shin Nakamura
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Publication number: 20040120034Abstract: An illumination apparatus for a microscope and an image processing apparatus using the illumination apparatus include a light source, a semi-transmissive mirror splitting a light beam from the light source into two beams of the first and second irradiation light, two excitation filters selecting the wavelengths of the first and second irradiation light, a semi-transmissive mirror synthesizing individual beams of the first and second irradiation light whose wavelengths are selected, into a single beam, a dichroic mirror directing a light beam synthesized by the semi-transmissive mirror toward a specimen and transmitting light from the specimen, an objective lens, cameras imaging fluorescent light from the specimen after being separated into fluorescent light excited by the first and second wavelengths, and an image processing section processing fluorescent images formed by imaging elements.Type: ApplicationFiled: November 20, 2003Publication date: June 24, 2004Applicants: THE INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH, Olympus CorporationInventors: Atsushi Miyawaki, Takashi Fukano, Yasushi Aono
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Publication number: 20040072156Abstract: The present invention relates to genetic polymorphism data, compositions and methods for detecting genetic polymorphisms, methods for evaluating drugs using genetic polymorphisms and screening methods for drugs.Type: ApplicationFiled: December 27, 2001Publication date: April 15, 2004Applicant: Riken Institute of Physical and Chemical ResearchInventors: Yusuke Nakamura, Akihiro Sekine, Aritoshi Ilda, Susumu Saito
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Publication number: 20030188971Abstract: Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. It fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.Type: ApplicationFiled: March 31, 2003Publication date: October 9, 2003Applicants: The Institute of Physical and Chemical Research, Shimadzu Corporation, Japan Science and Technology CorporationInventors: Yoshihide Hayashizaki, Rintaro Yamamoto
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Patent number: 6617424Abstract: Disclosed are peptides represented by IVC1SLC2SC3TAW and C1SLC2SC3 wherein I stands for isoleucine, V for valine, C1 for cysteine, C2 for cysteinesulfinic acid, C3 for cysteinesulfenic acid, S for serine, L for leucine, T for threonine, A for alanine, and W for tryptophan. These peptides can impart photoreactivity to a protein by binding to a non-heme iron. Also disclosed are methods for imparting photoreactivity to cells by introducing the peptide sequences into at least one protein which is involved in a metabolic system and/or energy metabolic system of the cells. There can be provided peptide sequences with a shorter peptide chain capable of imparting photoreactivity, which can be easily introduced into a protein with a little risk in degrading original function of the protein. There are also provided methods enabling control of metabolic reactions by imparting photoreactivity to cells with the peptides.Type: GrantFiled: March 14, 2001Date of Patent: September 9, 2003Assignee: The Institute of Physical and Chemical ResearchInventors: Isao Endo, Masafumi Odaka, Masafumi Yohda, Masayoshi Nakasako, Koji Takio
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Publication number: 20030113773Abstract: The present invention relates to neurogenesis inducing genes. In particular, the present invention provides neurogenesis inducing genes coding for Zic proteins, vectors containing such genes, host cells containing such vectors, proteins produced by such host cells, antibodies raised to such proteins, and therapeutic agents or agents for gene therapy for nervous diseases.Type: ApplicationFiled: September 16, 2002Publication date: June 19, 2003Applicant: THE INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCHInventors: Katsuhiko Mikoshiba, Jun Aruga, Takeharu Nagai, Katsunori Nakata
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Patent number: 6566497Abstract: In accordance with the present invention, there are provided isolated mammalian Acetyl-Coenzyme A Transporter (AT) proteins, anti-AT antibodies, therapeutic compositions, and nucleic acids encoding such. Bioassays and therapeutic methods employing invention AT proteins are also provided.Type: GrantFiled: September 2, 1998Date of Patent: May 20, 2003Assignees: The Burnham Institute, The Institute of Physical and Chemical ResearchInventors: Minoru Fukuda, Akiko Kanamori, Yoshio Hirabayashi
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Patent number: 6560859Abstract: Capillary columns (102) pass through and are inserted in a rubber plate (14), held and fixed by elastic force of rubber, and two-dimensionally arranged on a sample injection side. It fixes the capillary columns (102) arranged on a plane in close contact by holding the same with a holder plate (6a) from below and with a rubber plate (16) from above on a detection side. In order to press the capillary columns (102) against the holder plate 6a and fix the same with the rubber plate (16), a holder plate (6b) fixing the rubber plate (16) to the holder plate (6a) on both sides of the arrangement of the capillary columns (102) is provided.Type: GrantFiled: October 14, 1999Date of Patent: May 13, 2003Assignees: The Institute of Physical and Chemical Research Shimadzu Corporation, Japan Science and Technology CorporationInventors: Yoshihide Hayashizaki, Rintaro Yamamoto
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Patent number: 6528296Abstract: The present invention relates to a site-specific endonuclease which recognizes a specific nucleotide sequence, to a gene coding for the endonuclease, to a recombinant vector containing the gene, to a transformant containing the vector, and to a process for producing the endonuclease.Type: GrantFiled: August 20, 2001Date of Patent: March 4, 2003Assignee: Institute of Physical and Chemical ResearchInventors: Nobuhiro Morishima, Hikaru Mizumura, Takehiko Shibata