Abstract: One example of systems and methods for inertio-elastic focusing of particles in microchannels includes a substrate including a channel having an inlet and an outlet. A viscoelastic fluid, i.e., a fluid having a dynamic viscosity that varies with shear rate, and that carries suspended particles is driven through the channel. The volumetric flow rate at which the fluid is driven results in the formation of a localized pathline in the fluid at or near a center of the channel. The localized pathline defines a width that is equal to or slightly greater than a hydraulic diameter of the particle. The particles in the fluid are focused into the localized pathline.

Abstract: A single photon detector (SPD) includes a resonator to store probe photons at a probe wavelength and an absorber disposed in the resonator to absorb a signal photon at a signal wavelength. The absorber is also substantially transparent to the probe photons. In the absence of the signal photon, the resonator is on resonance with the probe photons, thereby confining the probe photons within the resonator. Absorption of the signal photon by the absorber disturbs the resonant condition of the resonator, causing the resonator to release multiple probe photons. A photodetector (PD) then detects these multiple probe photons to determine the presence of the signal photon.

Abstract: The systems and methods described herein are directed towards a solid state pumping system that utilizes an electric field applied across a channel formed within the solid state pump to move electro-rheological (ER) fluid from an inlet fluidly coupled to a first end of the channel to an outlet fluidly coupled to a second end of the channel. The solid state pumping system may include first, second and third plate with the second plate disposed between the first and third plate. The second plate may include a channel having first and second circuits coupled to opposing sides of the channel. In an embodiment, in response to a voltage applied thereto, the first and second circuits can provide an electric field voltage across the channel such that in response to the electric field voltage the ER fluid moves from the first end to the second end of the channel.

Abstract: Methods and apparatus of adding propellant to a thruster assembly are described. A first end of a beaker is disposed in an opening of the tank, where the beaker contains propellant and the first end of the beaker includes a breakaway bottom. The thruster assembly and beaker are placed in a first environment, where the first environment is substantially a vacuum and/or an environment composed substantially of gases that can be absorbed by the propellant. A plunger in the beaker is depressed to cause the breakaway bottom of the beaker to break and the propellant to flow into the tank of the thruster assembly. The thruster assembly is removed from the first environment and the beaker is removed from the opening. A cap is added to complete the assembly. The assembly contains a vent to allow gases to escape the interior of the tank.

Abstract: Aspects of the disclosure relate to methods and compositions of altering a solution through the use of readily retrievable agents (e.g., a nanoparticle) having one or more functional groups configured to undergo a solvation interaction with a component of the solution. Compositions, systems, and methods for crystallizing organic and inorganic compounds from solutions using nanoparticles surface coated with functional groups that create a supersaturated state in the solution and reversal thereof. Compositions, systems, and methods for purifying water or active pharmaceutical ingredients. Compositions, systems, and methods for increasing the solubility of a solution and reversal of the same. Compositions, systems, and methods for decreasing the solubility of a crystallized compound and reversal of the same.

Abstract: There is provided a shape memory alloy wire with a length of polycrystalline shape memory alloy having an alloy composition including at least one member selected from the group consisting of Cu in at least 10 wt. %, Fe in at least 5 wt. %, Au in at least 5 wt. %, Ag in at least 5 wt. %, Al in at least 5 wt. %, In in at least 5 wt. %, Mn in at least 5 wt. %, Zn in at least 5 wt. % and Co in at least 5 wt. %, and having a martensite crystal structure consisting of one of 2H, 18R1, M18R, and 6R. The length of polycrystalline shape memory alloy has a cross sectional wire diameter greater than 1 micron and less than 500 microns, an oligocrystalline morphology including polycrystalline grains that span the wire diameter and a wire surface with a surface roughness that is no greater than about 100 nanometers.

Abstract: A feed of at least one of (a) a source liquid including a solvent with a dissolved impurity and (b) a retentate of the source liquid is pumped in a substantially closed loop through a liquid-separation module. The liquid-separation module includes a membrane that passes at least partially purified solvent to a permeate side of the membrane while diverting the impurity in a retentate on the retentate side of the membrane. The purified solvent is extracted from the permeate side of the membrane; and the retentate from the liquid-separation module is pumped to or through a pressurized reservoir with a variable volume for the feed component and recirculated as a component of the feed. Over time, the volume for the feed is reduced and the pressure applied to the feed in the reservoir is increased to balance against an increasing difference in osmotic pressure across the membrane.

Abstract: A method for operating a secure device having a plurality of mutually exclusive circuit zones, including a first circuit zone having a first level of security and a second circuit zone having a second level of security less than the first level of security, the method including unpacking a key exchange package including receiving a key exchange package in the second circuit zone, the key exchange package including encrypted key data and processing the encrypted key data using a content key in the first circuit zone to generate decrypted key data and storing the decrypted key data in the first circuit zone without disclosing the decrypted key data into the second circuit zone.

Abstract: A thermogalvanic electrochemical system includes two battery sources connected together in a switchable circuit to provide electric current to an electrical load in the circuit. The battery sources are located in a thermal environment having a varying temperature, and each battery source has a corresponding electrical potential that varies with temperature of the thermal environment. A power management processor operates the circuit to: form a closed circuit at a first temperature with current flow from a first battery source through the electrical load to a second battery source until reaching a first thermal equilibrium, and then form an open circuit, then form a closed circuit at a second temperature with current flow from the second battery source through the electrical load to the first current source until reaching a second thermal equilibrium, and then form an open circuit.

Abstract: Security guards at large facilities, such as airports, monitor multiple screens that display images from individual surveillance cameras dispersed throughout the facility. If a guard zooms with a particular camera, he may lose image resolution, along with perspective on the surrounding area. Embodiments of the inventive Imaging System for Immersive Surveillance (ISIS) solve these problems by combining multiple cameras in one device. When properly mounted, example ISIS systems offer up to 360-degree, 240-megapixel views on a single screen. (Other fields of view and resolutions are also possible.) Image-stitching software merges multiple video feeds into one scene. The system also allows operators to tag and follow targets, and can monitor restricted areas and sound an alert when intruders breach them.

Abstract: An artificial saturable absorber uses additive pulse mode-locking to enable pulse operation of an on-chip laser operation. Four different artificial saturable absorbers are disclosed. The first includes an integrated coupler, two arms each containing some implementation of the end-reflector, and a phase bias element in one arm. The second includes an integrated directional coupler, two integrated waveguide arms, and another integrated coupler as an output. The third includes an integrated birefringent element, integrated birefringent-free waveguide, and integrated polarizer. And the fourth includes a multimode waveguide that allows for different modes to propagate in such a way that the difference in the spatial distribution of intensity causes a nonlinear phase difference between the modes.

Abstract: Emulsion-based and micromolded (“MM”) or three dimensional printed (“3DP”) polymeric formulations for single injection of antigen, preferably releasing at two or more time periods, have been developed. Formulations are preferably formed of biocompatible, biodegradable polymers. Discrete regions encapsulating antigen, alone or in combination with other antigens, adjuvants, stabilizers, and release modifiers, are present in the formulations. Antigen is preferably present in excipient at the time of administration, or on the surface of the formulation, for immediate release, and incorporated within the formulation for release at ten to 45 days after initial release of antigen, optionally at ten to 90 day intervals for release of antigen in one or more additional time periods. Antigen may be stabilized through the use of stabilizing agents such as trehalose glass. In a preferred embodiment for immunization against polio, antigen is released at the time of administration, and two, four and six months thereafter.

Abstract: The present disclosure provides a synthetic nanoparticle comprising a peptide nucleic acid (PNA) oligomer conjugated to a lipid, wherein the PNA oligomer noncovalently complexes with an immunomodulatory compound, thereby forming a nanoparticle. The nanoparticles are useful to elicit immune responses and can be used to treat a broad range of cancers and infectious diseases.

Abstract: The present invention provides, among other things, technologies and methodologies for detection, treatment, and/or prevention of influenza transmission and/or infection. The present invention also provides technologies for monitoring influenza variants for their potential to present a pandemic risk to humans.

Abstract: Apparatuses and methods for cell and tissue assays and agent delivery are generally described.

Abstract: An ionic liquid ion source can include a microfabricated body including a base and a tip. The body can be formed of a porous material compatible with at least one of an ionic liquid or room-temperature molten salt. The body can have a pore size gradient that decreases from the base of the body to the tip of the body, such that the at least one of an ionic liquid or room-temperature molten salt is capable of being transported through capillarity from the base to the tip.

Abstract: The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.

Abstract: Disclosed herein are methods of performing continuous directed evolution in complex biological systems, including metazoan cells. These methods include the infection of engineered, non-naturally occurring metazoan cells with engineered, non-naturally occurring DNA viruses. The generation of infectious viruses that can infect new cells depends on the evolution of a gene of interest which is driven by an error-prone adenoviral polymerase. Also disclosed herein, are the compositions of engineered, non-naturally occurring metazoan cells and engineered, non-naturally occurring DNA viruses that function as components in the continuous directed evolution methodologies.

Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a SIN CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing SIN CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: A bioconversion scheme is provided that effectively converts syngas, generated from gasification of coal, natural gas or biomass, into lipids that can be used for biodiesel production.