Abstract: A method based on polymerase chain reaction (PCR) amplification and oligonucleotide ligase assay (OLA) reaction is provided for analyzing complex genetic systems in a single reaction vessel. The method involves simultaneously incubating a sample containing one or more target polynucleotides with PCR primers and OLA probes in a single reaction mixture. The presence of variant polynucleotide sequences in the sample is determined by detecting and identifying the products of the OLA reaction.

Abstract: A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20K Daltons. Furthermore, the invention includes stable denaturants useful for the electrophoresis of nucleic acids.

Abstract: A method is provided for monitoring the progress of nucleic acid amplifications that rely on a nucleic acid polymerase having 5'.fwdarw.3' exonuclease activity. An important feature of the method is providing an oligonucleotide probe having a reporter molecule and a quencher molecule at either end such that the quencher molecule substantially quenches any fluorescence from the reporter whenever the oligonucleotide probe is in a single stranded state and such that the reporter is substantially unquenched whenever the oligonucleotide probe is in a double stranded state hybridized to a target polynucleotide.

Abstract: An optical switch includes a primary optical fiber terminating in a primary window, and a plurality of secondary optical fibers each terminating in a secondary window facing in the same direction as and spaced laterally from the primary window. A primary lens on a primary axis has a focal point positioned on the axis at the central window. Each of a plurality of secondary lenses has an axis and a focal point positioned on such axis at a secondary window. The secondary axes are parallel to and equidistant from the primary axis. A retroreflector is spaced from the lenses oppositely from each window and has an optical axis centered parallel to the primary axis midway between the primary axis and any of the secondary fibers. A stepper motor rotates the retroreflector about the primary axis to each of a plurality of selected positions to provide optical switching for light transmission between the primary fiber and any selected secondary fiber.

Abstract: A suspension system for isolating a platform from external vibrations includes spring assemblies suspending the platform. In each spring assembly an elongated outer coil spring is suspended at an upper end thereof from a support structure. A tube is disposed loosely within the outer spring and is attached to a lower end of the spring so as to extend upwardly. An elongated inner coil spring is disposed loosely within the tube and is suspended from an upper point of the tube. The inner spring has a lower end attached to the platform. For friction dampening, o-rings are fitted on the inner spring in sliding contact with the tube.

Abstract: A high pressure check valve assembly includes a ceramic cage loosely fitted into the bore of a body so as to leave at least a partial clearance between the cage and the body. A passage through the cage includes a retaining section adjacent a fluid inlet end for loosely containing a ball and an irregular cross-section adjacent the retaining section so as to obstruct the ball without hindering fluid flow forwardly through the passage. The cage further has a lateral bleed hole between the passage and the clearance to equalize fluid pressure. An outlet-end sealing member is press fitted into the bore against the outlet end of the cage. An oversized resilient washer-shaped inlet-end sealing member is press fitted into the bore against the inlet end of the cage to seal and retain the assembly. This member has a central aperture with a washer shaped seat member fitted therein, the seat member functioning with the ball as the check valve.

Abstract: A vacuum plasma spray system includes a spray chamber, a motor-driven manipulator including a drive mechanism and a respective control system, and a plasma gun mounted on the manipulator in the spray chamber. The drive mechanism comprises three drive groups of which a first drive group facilitates upward and downward motion of the gun for adjusting spray distance and second and third drive groups facilitate swiveling motions of the gun around horizontal axes. The manipulator has a hermetically sealed outer housing and a hermetically sealed inner housing contained in the outer housing. The inner housing contains the second and third drive groups, protrudes into the chamber and carries the gun. The first drive group is arranged at least partially in the outer housing to effect lifting or lowering of the inner housing thereby adjusting spray distance.

Abstract: The effective range of an analog linear-log converter is extended above the number of output decades over which this converter is reasonably linear to cover several additional decades of log conversion. This is made possible by adding a variable gain amplifier having several decades of switchable gain to the input of the converter. Switching between decades to utilize the added decades of output is controlled by a microcomputer which monitors the output level. This switching restores the signal level of each of the added decades to the same level as that of an earlier decade thus maintaining the same slope accuracy and noise levels of the earlier decades throughout the whole conversion range. The microcomputer also provides slope corrections from a lookup table for each of the different output decades as well as also applying to the output corrections for offsets due to the circuit components. Ambient temperature compensation is also provided following the linear-log converter for increased precision.

Abstract: A differential scanning calorimeter includes a baseline correction apparatus which simultaneously corrects for both linear and curvilinear heat flow variation between the sample and reference holders.

Abstract: This invention relates to a method and apparatus for measuring the exposure variation in the cross scan direction in an optical projector and scanning apparatus of the type that uses an annular aperture or slit. This measuring is achieved by measuring the light intensity from point to point along the arcuate illuminated area. Where the illuminated area is tapered so as to accommodate a pivoting carriage the system of this invention processes the intensity signal to yield a uniformity signal that only indicates departures from the ideal.

Abstract: A composite objective lens for a particle beam lithographic system having deflection coils within the bore of the lens, a solenoidal excitation lens coil, a first set of two or more cylindrical pole pieces with a lens gap and a second set of cylindrical pole pieces arranged concentrically outside the first pole pieces but within the excitation lens coil. The second set of pole pieces is constructed of a high saturation material such as iron with a lens gap coextensive with the gap in the first set of pole pieces. The return flux from the deflection coils is carried by the inner cylindrical pole pieces only, while the flux generated by the lens coil is shared by both the inner and outer pole pieces. With this arrangement the linear relationship between the axial field strength and excitation current is maintained at all points along the axis of the lens, and saturation of the inner pole piece is avoided.

Abstract: Disclosed is a regulator circuitry (stabilizer) for controlling the output of an RF generator according to a programmable input signal. A power stage is responsive to the selected value of the programmable input signal to drive the RF generator at the desired power level. A forward power feedback control circuit monitors and controls the power output stage so that the desired RF output level is maintained at a constant level. In this particular mode of operation, RF output power is maintained constant and therefore independent of variations in gain due to temperature changes and other parameters which could effect the overall transfer function of said generator (and including mismatch of the generator into the load).

Abstract: A particle beam lithographic system and method using smaller address structure particle beam for generating masks for integrated circuit technology utilizing an electrostatic deflector system to sweep the beam in the serpentine path for increased throughput.

Abstract: A reusable electric overlay measurement base pattern includes a pair of current pads forming first and second pads adapted to be contacted by point contacts, a first conductor of controlled width extending between the first and second pads, a pair of voltage pads forming third and fourth pads suitable for contacting by point contacts, two second conductors extending from the third and fourth pads to the first conductor contacting the first conductor at a predetermined spacing, and a fifth pad disposed adjacent and spaced from said first conductor approximately at the midpoint between the two second conductors. This permits a second level conductor to be deposited extending between the fifth pad and said first conductor which, when perfectly aligned, will intersect the first conductor at its midpoint. The misalignment of the second level conductor can be detected by measurements using the five pads.

Abstract: A transducer assembly comprising an inner, primary, transducer having a pair of transducers fixed to a liner or cavity for providing the fluid compression sending and receiving signals for measuring the velocity and change of composition of fluid in the cavity which is being inspired and expired by a patient in a setting, such as in an intensive care unit of a hospital. The inner transducer is disposed in a heated housing to maintain the temperature of the inner transducer at a temperature above the saturation point of the fluid being measured and is further provided with (1) an acoustical absorber to prevent sound energy from the connection tubes, which connect the transducer assembly to endotracheal tubing, from adversely affecting fluid measurements and, (2) water absorbing material to prevent water, which might condense in the connecting tubing, from entering the inner transducer and affecting the fluid measurements.

Abstract: Electrical connections to thin film coatings, especially useful in a fluid flowmeter, have a conductive coating pressed against a rigid, relatively long, connector bar by conductively coated pads of elastomeric material at a selected controlled pressure, with the electrical connections to the film coating being made through the bar and pads. Utilizing this method, localized high stress areas which cause damage to the thin film are avoided, and low current densities are accomplished by the controlled contact pressure over a large area of the film coating.

Abstract: A method of depositing refractory metal silicides in a sputtering system on one or more substrates in an environment which supports a glow discharge from a pair of targets at an energy level sufficient to co-sputter and deposit the material from the targets onto the substrate(s). The method includes either a RF high voltage to be applied to one of the targets, or a DC voltage in the presence of a magnetic field to be applied to both targets so as to deposit silicon, either doped or pure, and refractory metal, either doped or pure, as the case may be, to provide a thin film of doped refractory metal silicide on the substrate(s).

Abstract: An electrostatic cassette assembly comprising, a cassette body having a central opening for receiving a wafer to be processed in a particle beam lithographic system, a charge plate with a top surface having a coating of dielectric (nonconductive) material or a plurality of nonconductive means, or both, on which the wafer is supported, means for forcing said charge plate and wafer into contact with stop means to hold the wafer rigidly in said cassette assembly, and means for applying a potential difference between the wafer and the charge plate to flatten said wafer by Coulombic force so that the wafer is held both mechanically and electrostatically in said cassette assembly. Means are also provided for insuring a good electrical contact with the wafer and for aligning the wafer in the cassette assembly.

Abstract: Disclosed is a detector for determining the location of wafers moving in a sputtering system, for example, to and from the sputtering chamber where the wafers are processed, including a capacitive transducer, located in the path of travel of the wafers, to which is coupled an oscillator supplying a constant current thereto. This current is detected by a sensor, whose output is processed and applied to a comparator. The comparator is preferably preset to supply a digital "0" when the current through the transducer is at a static or no-wafer condition, and a digital "1" when the capacity of the transducer changes due to the presence of a wafer in close proximity to the transducer which is sensed as an increase in current which is reflected as a change of voltage level applied to the comparator.