Patents Assigned to The Salk Institute for Biological Studies
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Patent number: 12029594Abstract: A method to, is provided for collecting an image from a sample. The method includes selecting a radiation level for a first probe to meet a desired radiation dosage, and providing, with the first probe, a radiation at a selected point within a region of the sample. The method includes identifying a second selected point within the region of the sample based on a down sampling scheme, and providing a second radiation amount at the second selected point within the region of the sample. The method also includes interpolating a first datum and a second datum based on an up sampling scheme to obtain a plurality of data, and forming an image of the region of the sample with the plurality of data. A system to perform the above method and including the first probe is also provided.Type: GrantFiled: August 21, 2019Date of Patent: July 9, 2024Assignee: THE SALK INSTITUTE FOR BIOLOGICAL STUDIES, A CORPORATION OF CALIFORNIAInventors: Uri Manor, Linjing Fang
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Publication number: 20240216544Abstract: Provided herein are compositions and systems for reconstitution of RNA molecules, including methods for using these molecules. For example, such molecules can be used to deliver a protein coding sequence over two or more viral vectors (such as AAVs), resulting in reconstitution of the full-length protein in a cell. Such methods can be used to deliver a protein involved in editing a nucleic acid molecule, for example to treat a genetic disease or cancer.Type: ApplicationFiled: May 16, 2022Publication date: July 4, 2024Applicant: Salk Institute for Biological StudiesInventors: Lukas Christoph Bachmann, Samuel Lawrence Pfaff
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Publication number: 20240207445Abstract: Synthetic adenoviruses with liver detargeting mutations and expressing an adenovirus type 34 (Ad34) fiber protein, or a chimeric fiber protein with an Ad34 knob domain, are described. The synthetic adenoviruses traffic to sites of tumors. Use of the synthetic adenoviruses for delivering diagnostic or therapeutic transgenes to tumors are also described.Type: ApplicationFiled: September 29, 2023Publication date: June 27, 2024Applicant: Salk Institute for Biological StudiesInventors: Clodagh O'Shea, Colin Powers, Lei Zhang
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Publication number: 20240209354Abstract: Provided herein are multiplex crRNAs and multiplex sgRNAs, as well as RNA molecules thereof. Also provided are compositions and kits including the multiplex crRNAs and sgRNAs, which can be used in a multiplex targeted gene activation (mTGA) system. Also provided are methods that include administering a therapeutically effective amount of the mTGA system to a subject. In some examples, the method treats a disease associated with reduced or no expression of a gene, such as type I diabetes, Duchenne muscular dystrophy, a liver disease, or acute kidney disease.Type: ApplicationFiled: April 28, 2022Publication date: June 27, 2024Applicant: Salk Institute for Biological StudiesInventors: Juan Carlos Izpisua Belmonte, Chao Wang, Pradeep Reddy, Hsin-Kai Liao
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Publication number: 20240180882Abstract: The present disclosure provides compositions and methods for prevention or treatment of hearing loss. In some examples, a composition for preventing or treating hearing loss comprises at least one compound that activates AMPK in at least one hair cell.Type: ApplicationFiled: March 4, 2022Publication date: June 6, 2024Applicants: The Regents of the University of California, SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE, CALIFORNIA INSTITUTE OF TECHNOLOGY, THE SALK INSTITUTE FOR BIOLOGICAL STUDIESInventors: Rick A FRIEDMAN, Ely BOUSSATY, Andre HOELZ, Steven OLSON, Reuben SHAW
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Patent number: 11981931Abstract: The invention generally features compositions comprising induced pluripotent stem cell progenitors (also termed reprogramming progenitor cells) and methods of isolating such cells. The invention also provides compositions comprising induced pluripotent stem cells (iPSCs) derived from such progenitor cells. Induced pluripotent stem cell progenitors generate iPSCs at high efficiency.Type: GrantFiled: December 8, 2020Date of Patent: May 14, 2024Assignee: Salk Institute for Biological StudiesInventors: Ronald Evans, Michael Downes, Yasuyuki Kida, Teruhisa Kawamura, Zong Wei, Ruth T. Yu, Annette R. Atkins
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Publication number: 20240150755Abstract: Methods of modulating regulatory T (Treg) suppressor activity are provided. Also provided are methods of treating autoimmune diseases and methods of treating cancer. The methods include increasing or reducing the expression or activity of bromodomain-containing 9 (Brd9), bromodomain-containing 7 (Brd7), and/or polybromo 1 (Pbrm1) in a Treg cell or in a subject.Type: ApplicationFiled: February 25, 2022Publication date: May 9, 2024Applicant: Salk Institute for Biological StudiesInventors: Ye Zheng, Diana Hargreaves, Chin-San Loo, Jovylyn Gatchalian
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Patent number: 11970720Abstract: Provided herein are CRISPR/Cas methods and compositions for targeting RNA molecules, which can be used to detect, edit, or modify a target RNA.Type: GrantFiled: February 21, 2020Date of Patent: April 30, 2024Assignee: Salk Institute for Biological StudiesInventors: Patrick D. Hsu, Silvana Konermann
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Patent number: 11959094Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.Type: GrantFiled: May 2, 2023Date of Patent: April 16, 2024Assignee: Salk Institute for Biological StudiesInventors: Juan Carlos Izpisua Belmonte, Keiichiro Suzuki, Reyna Hernandez-Benitez, Jun Wu, Yuji Tsunekawa
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Publication number: 20240101962Abstract: The invention features pancreatic islet and pancreatic organoids, and cell cultures and methods that are useful for the rapid and reliable generation of pancreatic islet and pancreatic islet organoids. The invention also features methods of treating pancreatic diseases and methods of identifying agents that are useful for treatment of pancreatic diseases, such as type 2 diabetes and pancreatic cancer, using the pancreatic islet and pancreatic organoids of the invention.Type: ApplicationFiled: June 23, 2023Publication date: March 28, 2024Applicant: Salk Institute for Biological StudiesInventors: Ronald EVANS, Michael DOWNES, Annette ATKINS, Eiji YOSHIHARA, Ruth YU
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Publication number: 20240076612Abstract: Provided herein are, inter alia, are media compositions useful for culturing neural cells. In particular, the compositions provided herein mimic important physiological conditions in the living brain and sustain neural activity. The media compositions provided herein improve the efficiency of human neuron maturation and promote synaptic function in long-term in vitro cultures.Type: ApplicationFiled: April 11, 2023Publication date: March 7, 2024Applicant: SALK INSTITUTE FOR BIOLOGICAL STUDIESInventors: Fred H. Gage, Cedric Bardy
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Publication number: 20240017097Abstract: The invention provides compositions featuring TRP-4 polypeptides and polynucleotides, methods for expressing such polypeptides and polynucleotides in a cell type of interest, and methods for inducing the activation of the TRP-4 polypeptide in neurons and other cell types using ultrasound.Type: ApplicationFiled: August 31, 2023Publication date: January 18, 2024Applicant: Salk Institute for Biological StudiesInventors: Sreekanth H. CHALASANI, Stuart IBSEN
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Patent number: 11857809Abstract: The invention provides compositions featuring TRP-4 polypeptides and polynucleotides, methods for expressing such polypeptides and polynucleotides in a cell type of interest, and methods for inducing the activation of the TRP-4 polypeptide in neurons and other cell types using ultrasound.Type: GrantFiled: September 23, 2020Date of Patent: January 2, 2024Assignee: Salk Institute for Biological StudiesInventors: Sreekanth H. Chalasani, Stuart Ibsen
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Publication number: 20230399663Abstract: Disclosed herein are homology-independent targeted integration methods of integrating an exogenous DNA sequence into a genome of a non-dividing cell and compositions for such methods. Methods herein comprise contacting the non-dividing cell with a composition comprising a targeting construct comprising the exogenous DNA sequence and a targeting sequence, a complementary strand oligonucleotide homologous to the targeting sequence, and a nuclease, thereby altering the genome of the non-dividing cell.Type: ApplicationFiled: May 2, 2023Publication date: December 14, 2023Applicant: Salk Institute for Biological StudiesInventors: Juan Carlos Izpisua Belmonte, Keiichiro Suzuki, Reyna Hernandez-Benitez, Jun Wu, Yuji Tsunekawa
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Publication number: 20230364263Abstract: Synthetic adenoviruses with tropism to bone tissue are described. The synthetic adenoviruses include an adenovirus type 11 (Ad11) fiber protein or a chimeric adenovirus fiber protein having an Ad11 knob domain. The synthetic adenoviruses can also include a transgene, such as a reporter gene or a transgene encoding a factor that promotes bone regeneration or repair. Use of the synthetic adenoviruses to target bone tissue and/or to promote bone repair or regeneration is also described.Type: ApplicationFiled: April 19, 2023Publication date: November 16, 2023Applicant: Salk Institute for Biological StudiesInventors: Clodagh O'Shea, Colin Powers, Lei Zhang
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Patent number: 11813337Abstract: Synthetic adenoviruses with liver detargeting mutations and expressing an adenovirus type 34 (Ad34) fiber protein, or a chimeric fiber protein with an Ad34 knob domain, are described. The synthetic adenoviruses traffic to sites of tumors. Use of the synthetic adenoviruses for delivering diagnostic or therapeutic transgenes to tumors are also described.Type: GrantFiled: June 7, 2019Date of Patent: November 14, 2023Assignee: Salk Institute for Biological StudiesInventors: Clodagh O'Shea, Colin Powers, Lei Zhang
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Publication number: 20230340534Abstract: Provided and described are mechanosensory polypeptides and encoding polynucleotide products and compositions thereof, methods of expressing such polypeptides and polynucleotides in a cell type of interest, and methods of inducing and/or modifying the activity and/or function of various types of cells that express the exogenous mechanosensory polypeptides using ultrasound.Type: ApplicationFiled: May 10, 2023Publication date: October 26, 2023Applicants: Salk Institute for Biological Studies, The Scripps Research InstituteInventors: Sreekanth CHALASANI, Carl PROCKO, Joanne CHORY, Jose Mendoza LOPEZ, Marc Duque RAMIREZ, Corinne LEE-KUBLI, Swetha MURTHY, Ardem PATAPOUTIAN, Seyed Ali Reza MOUSAVI, William T. KEENAN, Yusuf TUFAIL, Janki PATEL
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Publication number: 20230340408Abstract: Provided and described are bacterial mechanosensory polypeptide and encoding polynucleotide products and compositions thereof, methods of expressing such polypeptides and polynucleotides in a cell type of interest, and methods of inducing and/or modifying the activity or function of various types of cells, including neurons, which express exogenous bacterial mechanosensory polypeptides, using ultrasound.Type: ApplicationFiled: May 2, 2023Publication date: October 26, 2023Applicants: Salk Institute for Biological Studies, The Regents of the University of CaliforniaInventors: Sreekanth CHALASANI, Corinne LEE-KUBLI, Uri MAGARAM, Daniel GIBBS
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Publication number: 20230328020Abstract: Provided herein are CRISPR/Cas methods and compositions for targeting RNA molecules, which can be used to detect, edit, or modify a target RNA.Type: ApplicationFiled: May 26, 2023Publication date: October 12, 2023Applicant: Salk Institute for Biological StudiesInventors: Patrick D. Hsu, Silvana Konermann
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Patent number: 11760977Abstract: The invention features pancreatic islet and pancreatic organoids, and cell cultures and methods that are useful for the rapid and reliable generation of pancreatic islet and pancreatic islet organoids. The invention also features methods of treating pancreatic diseases and methods of identifying agents that are useful for treatment of pancreatic diseases, such as type 2 diabetes and pancreatic cancer, using the pancreatic islet and pancreatic organoids of the invention.Type: GrantFiled: May 24, 2017Date of Patent: September 19, 2023Assignee: SALK INSTITUTE FOR BIOLOGICAL STUDIESInventors: Ronald Evans, Michael Downes, Annette Atkins, Eiji Yoshihara, Ruth Yu