Abstract: This disclosure provides systems and methods for attaching nanopore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disclosed tagged nucleotides.
Type:
Application
Filed:
July 1, 2022
Publication date:
January 12, 2023
Applicants:
The Trustees of Columbia University in the City of New York, Roche Sequencing Solutions, Inc.
Inventors:
Carl W. Fuller, Shiv Kumar, Jingyue Ju, Randall Davis, Roger Chen
Abstract: The disclosed subject matter provides for genetically modified cells, e.g., fungal cells, that autonomously generates and/or secretes one or more therapeutic molecules, e.g., therapeutic peptides, therapeutic proteins or small therapeutic molecules, in situ. In certain embodiments, the present disclosure provides genetically-engineered fungal cells that generate and secrete tetracycline and analogues thereof.
Type:
Application
Filed:
May 6, 2022
Publication date:
January 5, 2023
Applicant:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Abstract: The present invention relates to utilizing engineered horizontal gene transfer elements and high-throughput selection strategies to tag and retrieve genetically modified native commensal strains from the mammalian gut. In certain aspects, the present invention relates to methods wherein isolated bacteria from the mammalian gut microbiome that were amenable to genetic manipulation were redeployed back into the mammalian subject as host-optimized engineerable probiotics.
Type:
Application
Filed:
January 13, 2020
Publication date:
December 22, 2022
Applicant:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Abstract: The present subject matter relates to techniques for harmonic motion elastography. The disclosed system can include a focused ultrasound (FUS) transducer for applying a push to a target tissue; an imaging transducer for obtaining radio frequency (RF) signals from the target tissue, and a processor configured to estimate the mechanical properties of the target tissue by extracting a shear wave from the RF signals and estimating a shear wave speed.
Type:
Application
Filed:
May 6, 2022
Publication date:
December 22, 2022
Applicant:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors:
Elisa E. KONOFAGOU, Alireza NABAVIZADEHRAFSANJANI
Abstract: According to exemplary embodiments of the present disclosure, it is possible to provide method, system, arrangement, computer-accessible medium and device to stimulate individual neurons in brain slices in any arbitrary spatio-temporal pattern, using two-photon uncaging of photo-sensitive compounds such as MNI-glutamate and/or RuBi-Glutamate with beam multiplexing. Such exemplary method and device can have single-cell and three-dimensional precision. For example, by sequentially stimulating up to a thousand potential presynaptic neurons, it is possible to generate detailed functional maps of inputs to a cell. In addition, it is possible to combine this exemplary approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity. Further exemplary embodiments of the present disclosure can include a light-weight, compact portable device providing for uses in a wide variety of applications.
Type:
Grant
Filed:
December 18, 2017
Date of Patent:
December 20, 2022
Assignee:
The Trustees of Columbia University in the City of New York
Inventors:
Volodymyr Nikolenko, Rafael Yuste, Brendon O. Watson, Darcy Peterka
Abstract: The present invention relates to novel compounds and pharmaceutical compositions thereof, and methods for promoting healthy aging of skin, the treatment of skin disorders, the treatment of cardiovascular disorders, the treatment of renal disorders, the treatment of angiogenesis disorders, such as cancer, treatment of tissue damage, such as non-cardiac tissue damage, the treatment of evolving myocardial infarction, and the treatment of various other disorders, such as complications arising from diabetes with the compounds and compositions of the invention. Other disorders can include, but are not limited to, atherosclerosis, coronary artery disease, diabetic nephropathy, diabetic neuropathy, diabetic retinopathy, infections of the skin, peripheral vascular disease, stroke, and the like.
Type:
Grant
Filed:
October 10, 2019
Date of Patent:
December 20, 2022
Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors:
Andrew Wasmuth, Donald W. Landry, Shixian Deng, Banavara L. Mylari, Ravichandran Ramasamy, Ann Marie Schmidt
Abstract: The present disclosure provides for a method of determining microbial identities and/or abundances in a biological sample. The method may comprise: (a) immobilizing the biological sample in a matrix; (b) fracturing/breaking the matrix (that comprises the biological sample) into clusters; and (c) determining identities and/or abundances of microbes in the clusters.
Type:
Grant
Filed:
March 27, 2019
Date of Patent:
December 13, 2022
Assignee:
The Trustees of Columbia University in the City of New York
Abstract: An exemplary system for generating an image(s) of a sample(s) can include, for example, an imaging arrangement that can include a superluminescent diode (SLD) configured to generate a radiation(s) to be provided to the sample(s), and a spectrometer configured to (i) sample an A-line sampling rate of at least about 200 kHz, (ii) receive a resultant radiation from the sample(s) based on the sampling rate, and (iii) generate information based on the resultant radiation, and a computer hardware arrangement configured to generate the image(s) of the sample(s) based on the information received from the spectrometer. The imaging arrangement can be an interferometric imaging arrangement, which can be an optical coherence tomography imaging (OCT) arrangement. The computer hardware arrangement can be further configured to facilitate a plurality of b-scan acquisitions of the sample(s) and facilitate the b-scan acquisitions in order to generate the image(s).
Type:
Grant
Filed:
January 29, 2021
Date of Patent:
December 6, 2022
Assignee:
The Trustees of Columbia University in the City of New York
Inventors:
Christine Hendon, Richard Ha, Diana Mojahed, Hanina Hibshoosh, James McLean
Abstract: The invention provides various orthogonal nucleotide analogues and methods for using combinations of said various orthogonal nucleotide analogues for sequencing by synthesis.
Type:
Application
Filed:
July 9, 2020
Publication date:
November 24, 2022
Applicant:
The Trustees of Columbia University in the City of New York
Abstract: Light Sheet Theta (LS-0) Microscopy achieves large sample imaging capabilities without affecting the imaging depth or the image quality. An optical layout places a detection objective normal to the sample surface, while placing the illumination objectives that generate light sheets at an angle (theta) significantly smaller than 90 degrees. In this configuration, the light sheets enter from same side of the sample as the detection objective. The intersection of the light-sheet and the detection focal plane results in a line illumination-detection profile that is discriminated by a camera.
Type:
Grant
Filed:
November 10, 2017
Date of Patent:
November 22, 2022
Assignee:
The Trustees of Columbia University in the City of New York
Abstract: The present invention relates to detecting serum anti-FadA antibodies in test samples from a patient. Additionally, aspects of the present invention provide the basis for detection of serum anti-FadA antibody levels from test samples and correlation with various conditions of clinical relevance.
Type:
Grant
Filed:
May 3, 2018
Date of Patent:
November 22, 2022
Assignee:
The Trustees of Columbia University In the City of New York
Abstract: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded
Type:
Application
Filed:
July 7, 2022
Publication date:
November 17, 2022
Applicant:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors:
Jingyue Ju, Shiv Kumar, James J. Russo, Steffen Jockusch, Zengmin Li, Xiaoxu Li, Sergey M. Kalachikov, Irina Morozova
Abstract: Disclosed are devices, systems, apparatus, circuits, methods, products, and other implementations, including a method that includes obtaining, during execution of a process associated with a particular privilege level, data content from a memory location, and determining by a hardware-based detection circuit whether the data content matches at least one of one or more token values, with the one or more token values stored in one or more pre-determined memory locations, and with access of any of the pre-determined one or more memory locations indicating a potential anomalous condition. The method further includes triggering, in response to a determination that the data content matches the at least one of the one or more token values, another process with a higher or same privilege level as the particular privilege level associated with the process, to handle occurrence of a potential system violation condition.
Type:
Grant
Filed:
September 7, 2018
Date of Patent:
November 15, 2022
Assignee:
The Trustees of Columbia University in the City of New York
Abstract: This disclosure is related to a method of sequencing a single-stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore.
Type:
Grant
Filed:
October 15, 2019
Date of Patent:
November 15, 2022
Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors:
Jingyue Ju, Shiv Kumar, Zengmin Li, Chuanjuan Tao, Minchen Chien, James J. Russo, Sergey Kalachikov, Ken Shepard, Jacob Karl Rosenstein
Abstract: The present application discloses methods, devices, and systems for generating spatially-resolved quantification of cross-linking in cartilage based on Raman scattering of excitation light. The examples presented demonstrate that the method provides a discriminating power sufficient to distinguish early onset of osteo-arthritis even before a patient is symptomatic.
Type:
Grant
Filed:
June 10, 2017
Date of Patent:
November 15, 2022
Assignee:
The Trustees of Columbia University in the City of New York
Abstract: The present disclosure relates to novel compounds and pharmaceutical compositions thereof, and methods for promoting healthy aging of skin, the treatment of skin disorders, the treatment of cardiovascular disorders, the treatment of renal disorders, the treatment of angiogenesis disorders, such as cancer, treatment of tissue damage, such as non-cardiac tissue damage, the treatment of evolving myocardial infarction, the treatment of ischemic injury, and the treatment of various other disorders, such as complications arising from diabetes with the compounds and compositions of the invention. Other disorders can include, but are not limited to, atherosclerosis, coronary artery disease, diabetic nephropathy, diabetic neuropathy, diabetic retinopathy, diabetic cardiomyopathy, infections of the skin, peripheral vascular disease, stroke, asthma, and the like.
Type:
Grant
Filed:
November 2, 2020
Date of Patent:
November 15, 2022
Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Abstract: The present invention provides organic compounds having pseudocapacitive performance and methods of preparing said compounds. The organic compounds can include perylene diamine (PDI) subunits and hexaazatrinaphthylene (HATN) subunits.
Type:
Grant
Filed:
February 19, 2021
Date of Patent:
November 8, 2022
Assignee:
The Trustees of Columbia University in the City of New York
Inventors:
Colin Nuckolls, Xavier Roy, Jake Carter Russell, Samuel R. Peurifoy
Abstract: Methods for prophylactically treating a stress-induced affective disorder or stress-induced psychopathology in a subject are provided. Also provided are methods for inducing and/or enhancing stress resilience in a subject. In certain embodiments, an effective amount of (R,S)-ketamine or (2R, 6R)-hydroxynorketamine ((2R, 6R)-HNK) is administered to a female or male subject prior to a stressor.
Type:
Grant
Filed:
November 9, 2018
Date of Patent:
November 8, 2022
Assignees:
The Trustees of Columbia University in the City of New York, The Research Foundation for Mental Hygiene, Inc.
Inventors:
Christine Ann Denny, Briana Kaying Chen