Abstract: Provided herein is a method for diagnosing and treating a subject at risk for atrial fibrillation (AF) or related health conditions, the method including: collecting one or more physiological signals from the subject in a sleep state or an awake state; extracting time series data from the one or more physiological signals; performing dynamic analyses of the time series data using artificial intelligence, wherein the artificial intelligence calculates a series of dynamic measurements, said dynamic measurements being indicative of a probability of an onset of an abnormal atrial rhythm; providing an integrated personalized risk score including the dynamic measurements, wherein the integrated personalized risk score is indicative of a probability of an onset of AF in the subject; diagnosing the subject as being at risk for AF when the integrated personalized risk score exceeds a threshold value, wherein the threshold value is calculated by the artificial intelligence based on a library of stored data; and treatin

Abstract: Methods for applying a coating to a surface of a substrate for inhibition and prevention of implant-associated complications (including implant-associated infections), methods for inhibiting and preventing implant-associated complications (including implant-associated infections), implant-associated infection inhibiting coatings, and coated devices are provided. Coating processes include a) providing a saturated or supersaturated solution of an antibiotic in a fast-evaporating or medium-evaporating organic solvent; b) coating the surface of the substrate with at least one application of solution, each application followed directly by a solvent evaporation period.

Abstract: An electrochemical aptamer-based (E-AB) sensor is disclosed. The sensor is a closed bipolar electrode having a first end and a second end. The first end comprises an electrochromic material. The second end comprises an electrocatalyst and an oligonucleotide aptamer tethered to the second end. Further, the oligonucleotide aptamer is labelled with a redox indicator.

Abstract: Methods for preserving catalytic activity of a PSA polymer membrane in a humid environment by immobilizing in the membrane an organic acid having a pKa greater than the pKa of the PSA polymer membrane; optical sensors based on the PSA membranes further including an immobilized organic reagent capable of reacting with a target compound in a humid environment to produce a detectable color shifted product; and non-invasive methods for estimating blood glucose concentration by utilizing an optical sensor to detect concentration of acetone in exhaled human breath and correlating it to blood glucose concentration.

Abstract: Methods and compositions for treating a skin blistering disease are disclosed. In one embodiment, a method of treating a subject suffering from a skin blistering disease is disclosed. The method involves identifying an epithelial cell adhesion molecule associated with the skin blistering disease (EpCAM); then identifying a non-pathogenic monoclonal antibody (mAb) that targets the EpCAM; and then administering to the subject a therapeutically effective amount of the mAb.

Abstract: Compositions including modified adeno-associated virus (AAV) vectors comprising a recombinant AAV (rAAV)-based genome are provided herein, wherein the rAAV-based genome includes one or more of: a brain derived neurotrophic factor (BDNF)-encoding cDNA insert; or a neurotrophin-3 (NT-3)-encoding cDNA insert. Also provided are methods of treating motor neuron degenerative disorders, such as amyotrophic lateral sclerosis (ALS), by administering the disclosed compositions.

Abstract: Biological chemicals, potentially found in blood are measured by collecting sweat and determining the concentration or meaning of the selected chemical in sweat. The sweat can be collected using a time based, interval collector and analyzed using an external device. It can also be collected on a one time basis, using a flexible, chemical capacitor, or on a continuous basis using a chemical, field effect transducer.

Abstract: Devices and methods are described herein for directly and accurately measuring sweat flow rates using miniaturized thermal flow rate sensors. The devices (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500) include the flow rate sensors (220, 320, 420, 520, 620, 720, 820, 920, 1020, 1120, 1220, 1320, 1420) in or adjacent to a microfluidic component (230, 330, 430, 530, 630, 730, 830, 930, 1030, 1130, 1230, 1330, 1430, 1530) of a wearable sweat sensing device. The devices and methods optimize the sensitivity of the flow rate sensors, while minimizing the presence of noise, in order to accurately and directly measure sweat flow rates.

Abstract: A kit for preparing a nucleic acid sample for analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) is provided, the kit comprising: a lyophilized enzyme composition comprising: micrococcal nuclease; nuclease P1; and bacterial alkaline phosphatase (BAP); and a digestion buffer. Also provided are enzyme compositions and methods of use for rapid, efficient preparation of a nucleic acid sample for analysis by LC-MS/MS, without the need for denaturation of the sample.

Abstract: Provided herein are photocatalytic carbon filters for the removal impurities such as microorganisms, organic compounds, algal toxins, and their degradation by-products from water and wastewater. The photolytic carbon filters comprise a porous titanium substrate comprising TiO2 nanotube arrays and multi-wall carbon nanotubes disposed on the TiO2 nanotube arrays. Also provided herein are methods of manufacture and methods of use of the disclosed photocatalytic carbon filters.

Abstract: A genetically modified strain of commensal bacteria Staphylococcus epidermidis is provided which produces a material with UV absorbing properties. The material with UV absorbing properties may be a mycosporine-like amino acid such as shinorine.

Abstract: An electrode includes an insulating surface layer and at least one aligned carbon nanotube fiber embedded in the insulating surface layer. Each of the at least one aligned carbon nanotube fiber has a first end and a second end opposite the first end, and the first end and the second end are separated by a body. Each of the at least one aligned carbon nanotube fiber is composed of a plurality of carbon nanotubes. The first end and the second end are free of the insulating surface layer. The second end is in contact with an electrical conductive material. A method of analyzing an analyte in a sample and a device for energy storage using the electrode are also described.

Abstract: Described are a device and method for detecting cerebral perfusion in a subject's head during a cardiac crisis. Embodiments of the device include a neurological status evaluation apparatus having a signal generator configured to generate an electromagnetic signal at one or more frequencies, a transmitting antenna coupled to the signal generator and configured to transmit the electromagnetic signal, and a receiving antenna positioned proximate to the transmitting antenna such that an evaluation space is defined between the transmitting antenna and the receiving antenna. In embodiments, the subject's head does not contact the transmitting antenna or the receiving antenna. The receiving antenna receives a modulated electromagnetic signal after propagating through the subject's head. The neurological status evaluation apparatus further includes a signal analyzer coupled to the receiving antenna, wherein the signal analyzer receives and samples the modulated electromagnetic signal.

Abstract: A method for pretreating lignocellulosic biomass to improve fermentability is disclosed. The method involves reacting lignocellulosic biomass with an acidic mixture while heating at a temperature from about 120° C. to about 200° C. to produce a prehydrolysate mixture. The acidic mixture includes a dilute acid and nucleophilic proteins, hydrolyzed amino acids or combinations thereof.

Abstract: A composition includes an aqueous solution including at least one fluorinated organic compound; and a reaction product of a source of iron(II) and a source of sulfite in the presence of water and molecular hydrogen. A method of making a catalyst includes reacting a source of iron(II) and a source of sulfite in the presence of water and molecular hydrogen. The water may include less than or equal to 1 weight percent dissolved molecular oxygen. The catalyst may be used for degrading fluorinated organic chemicals in aqueous media.

Abstract: Systems and methods of use and fabrication are described for a THx2 threshold gate cell for a programmable gate array including a mode-independent PMOS configuration and an NMOS configuration configured to operate in one of a TH12 mode and a TH22 mode, wherein x is set to a threshold of 1 for the TH12 mode and x is set to a threshold of 2 for the TH22 mode.

Abstract: A method of treating a subject suffering from a condition characterized by over-expression of Ten-eleven translocation 1 (TET1) is provided, the method including administering to the subject an effective amount of a compound, or a pharmaceutically acceptable salt thereof, having the Formula I: wherein: R1, R2, R3, R4, and R5 are each independently selected from the group consisting of H, hydroxyl, alkyl, alkoxy, amine, halo, and trifluoromethyl, and wherein any two adjacent moieties of R1, R2, R3, R4, and R5 may come together to form a heterocyclic ring; R6 is H or hydroxyl; and R7 is selected from H, wherein R8 is C or O. Also provided are methods for selectively suppressing transcription of TET1 and/or reducing a level of 5-hydroxymethylcytosine in a subject by administering an effective amount of a Formula I compound and pharmaceutical compositions comprising Formula I compounds.

Abstract: Various aspects of the disclosure relate to a tracheal implant comprising (a) a trachea processed to comprise one or more incisions providing a helical configuration to the processed trachea; and (b) a subject-specific synthetic support.

Abstract: Provided herein is a method of diagnosing and treating a subject suffering from atopic dermatitis (AD), the method comprising: (a) obtaining a skin biopsy from a subject suspected of suffering from AD; (b) determining a level of RNA expression in the skin biopsy of genes selected from the 89ADGES gene panel; (c) comparing the determined level of RNA expression of the selected genes to the level of RNA expression of the selected genes in a reference sample comprising RNA expression products from normal healthy skin cells; (d) diagnosing the subject as suffering from AD when specific genes are up-regulated compared to the reference sample and when specific genes are down-regulated compared to the reference sample; and (e) treating the subject with a therapy effective for the treatment of AD. Methods of managing treatment of a subject suffering from AD are also provided.

Abstract: A method of characterizing nanovesicles is disclosed. The method involves entrapping nanovesicles such as exosomes and sensing the dielectric properties of the exosomes using an electrical impedance sensing device. The method can distinguish exosomes based on different membrane compositions, different cellular origins, different size distribution and/or different cytosolic compositions.