Abstract: Novel metabolites and enzymes involved in skatole metabolism are disclosed. The novel metabolites are 3-OH-3-methylindolenine (HMI); 3-methyloxindole (3MOI); indole-3-carbinol (I-3C); and 2-aminoacetophenone (2-AM). Measuring levels of these metabolites in a pig may be useful in identifying the pig's ability to metabolize skatole and its susceptibility to boar taint. The novel enzymes involved in skatole metabolism are aldehyde oxidase and CYP2A6. Enhancing the activity of these enzymes may be useful in enhancing skatole metabolism and reducing boar taint. The identification of the enzyme also allows the development of screening assays for substances that interact with these enzymes and skatole metabolism or for genetic screening to identify pigs on the basis of their skatole metabolism. Pigs having high levels of these enzymes may be selected and bred to produce pigs that have a lower incidence of boar taint.
Abstract: Flattening total current consumption of system having processing core and power supply input by current sensing within system at power supply input and controlling system current consumption such that system current is reduced if over reference current threshold, and increased if below reference current threshold. Inject additional current through digital injections cells working higher frequencies, by increasing switching activity, by increasing voltage supply to core, and by increasing operating frequency of processor core. Feedback signal indicates current consumption of system. Current consumption similarly decreased. Current sensed by mirroring input current inline with power supply input and compensating for voltage drop introduced by mirroring using opposing field effect transistors and maintaining outputs at same voltage through feedback control loop. Processor core may be general purpose processor core or cryptographic processor core. System may be system-on-chip or system-on-package.
Abstract: The invention discloses compositions for the preservation of fruits, vegetables, partially processed products, other produce and followers. The compositions comprise at least one phospholipase D inhibitor, at least one compound comprising an isoprene subunit, at least one component of the flavonoid biosynthetic pathway in a suitable medium. The composition of the present invention may additionally comprise one or more plant growth regulators of the cytokinin type, one or more antioxidants, a membrane stabilizing agent, a surfactant, or any combination thereof. The composition may be applied to produce as a spray, drench, dip, or a vapour and at either the pre-harvest stage or post-harvest stage.
Abstract: Novel metabolites and enzymes involved in skatole metabolism are disclosed. The novel metabolites are 3-OH-3-methylindolenine (HMI); 3-methyloxindole (3MOI); indole-3-carbinol (I-3C); and 2-aminoacetophenone (2-AM). Measuring levels of these metabolites in a pig may be useful in identifying the pig's ability to metabolize skatole and its susceptibility to boar taint. The novel enzymes involved in skatole metabolism are aldehyde oxidase and CYP2A6. Enhancing the activity of these enzymes may be useful in enhancing skatole metabolism and reducing boar taint. The identification of the enzyme also allows the development of screening assays for substances that interact with these enzymes and skatole metabolism or for genetic screening to identify pigs on the basis of their skatole metabolism. Pigs having high levels of these enzymes may be selected and bred to produce pigs that have a lower incidence of boar taint.
Abstract: The invention provides a transgenic animal having within its genome a transgene construct for gastrointestinal tract specific expression of a protein. In a preferred embodiment, the protein is a phytase or a homologue thereof. Such proteins may be heterologous and may be specifically expressed in the salivary gland of the animal by operably linking the nucleic acid sequence encoding the protein with regulatory sequence including a salivary gland protein promoter/enhancer. Also provided are methods of expressing and producing proteins using such nucleic acid constructs. Further, antibodies specific to such proteins and immunological diagnostic kits are also provided.
Type:
Grant
Filed:
April 20, 2000
Date of Patent:
October 3, 2006
Assignee:
University of Guelph
Inventors:
Cecil W. Forsberg, Serguei Golovan, John P. Phillips
Abstract: The present invention relates to methods of monitoring the menstrual cycle and/or the first half of pregnancy in a female by assessing the adhesion of lymphocytes from the female to uterine or lymphoid histological tissues from a pregnant animal or an animal that has been treated with gestational hormones. The method can be used to determine the ability of the lymphocyte donor's immune system to recognize and respond to an environment suitable for sustaining a pregnancy.
Type:
Grant
Filed:
October 25, 2002
Date of Patent:
August 22, 2006
Assignees:
University of Guelph, Health Research, Inc., Roswell Park Division
Abstract: The present invention relates to novel inducible genes from alfalfa. These genes are highly induced following mechanical harvesting or wounding of alfalfa. The genes are useful for inducible production of heterologous proteins following harvesting.
Abstract: Novel metabolites and enzymes involved in skatole metabolism are disclosed. The novel metabolites are 3-OH-3-methylindolenine (HMI); 3-methyloxindole (3MOI); indole-3-carbinol (I-3C); and 2-aminoacetophenone (2-AM). Measuring levels of these metabolites in a pig may be useful in identifying the pig's ability to metabolize skatole and its susceptibility to boar taint. The novel enzymes involved in skatole metabolism are aldehyde oxidase and CYP2A6. Enhancing the activity of these enzymes may be useful in enhancing skatole metabolism and reducing boar taint. The identification of the enzyme also allows the development of screening assays for substances that interact with these enzymes and skatole metabolism. Pigs having high levels of these enzymes may be selected and bred to produce pigs that have a lower incidence of boar taint.
Abstract: The development of an in vitro regeneration system that utilizes a plant growth regulator having cytokinin activity for the induction of de novo shoots or somatic embryos on explants of phytopharmaceutical plants is provided. Transfer of the regenerated shoots or somatic embryos into a solid or liquid medium with no plant growth regulators results in the rapid and prolific growth of viable plantlets. The method and its modifications are intended for application to all phytopharmaceutical plants, in particular St. John's wort (Hypericum perforatum cv. Anthos), Huang-qin (Scutellaria baicalensis), Echinacea sp., Feverfew (Tanacetum parthenium), garlic (Allium sp.) and the like. Furthermore, a process for the uptake of nutrients, minerals or additives from the growth medium and accumulation of these in the consumable biomass of plants, hereafter referred to as phytofortification, is also described.
Type:
Grant
Filed:
March 24, 2000
Date of Patent:
February 28, 2006
Assignee:
University of Guelph
Inventors:
Praveen K. Saxena, Susan J. Murch, Sankaran Krishnaraj, Tannis Y. Slimmon
Abstract: The invention pertains to the identification and isolation of a gene involved in the boar taint phenotype. A porcine form of a gene, designated SULT2A1, is identified. The invention additionally provides methods of inhibiting or reducing boar taint using the novel SULT2A1 gene or gene product. Methods for reducing or inhibiting boar taint and methods for screening compounds to modulate SULT2A1 are also disclosed.
Abstract: An immunovariant strain of Eimeria maxima was isolated. Vaccines incorporating the immunovariant strain are effective in eliciting immunological protection against coccidial infection.
Type:
Grant
Filed:
April 20, 2001
Date of Patent:
November 29, 2005
Assignees:
The United States of America as represented by the Secretary of the Agriculture, University of Guelph
Inventors:
Harry D. Danforth, M. Aggie Fernando, John R. Barta
Abstract: A method of preventing or reducing boar taint is described. The method involves increasing the metabolism of skatole in a pig by modulating the activity or expression of the enzymes involved in skatole metabolism.
Type:
Application
Filed:
May 20, 2005
Publication date:
September 29, 2005
Applicant:
UNIVERSITY OF GUELPH
Inventors:
E. Squires, Jakub Babol, Kerstin Lundstrom
Abstract: The laminin ?2 subunit of equine laminin-5 gene has been cloned and the nucleic acid and corresponding protein amino acid sequence is provided. A method of diagnosing junctional epidermolysis bullosa in horses is also provided based on the determination that a mutation in the laminin ?2 gene in which a cytosine is inserted at position 1368 is associated with the disease.
Abstract: The invention relates to novel vaccines and pharmaceutical compositions using membrane vesicles of microorganisms, methods for preparing same, and their use in the prevention and treatment of infectious diseases.
Type:
Grant
Filed:
September 6, 2002
Date of Patent:
July 12, 2005
Assignee:
University of Guelph
Inventors:
Jagath L. Kadurugamuwa, Terry J. Beveridge
Abstract: The present invention relates to a method for the production of a heterologous protein or peptide in a plant or a plant cell. The method involves providing a plant or a plant cell comprising construct comprising a harvest-inducible regulatory element that is operably linked with a heterologous coding sequence of interest, growing the plant or plant cell, and applying ABA or an analog thereof to the plant or plant cell, thereby inducing expression of the heterologous protein or peptide. If a plant is used, then following the application of ABA or an analogue thereof, the plant may be harvested.
Abstract: Disclosed herein are novel alleles characterized by polymorphisms in sulfotransferase genes. The alleles may be used to genetically type animals for sulfotransferase activity. In a preferred embodiment, the alleles may be used as markers for boar taint in pigs. Methods for identifying such markers, and methods of screening animals to determine those more likely to produce desired characteristics and preferably selecting those animals for future breeding purposes are also disclosed.
Abstract: Described are a novel gene (waaP) and a protein (WaaP) which has an enzymatic activity that is involved in heptose modification in the lipopolysaccharide of bacterial membranes. Also described is a method and series of compounds which are active against bacterial pathogens wherein the method comprises the administration of sufficient amount of an inhibitor of WaaP protein activity in gram negative bacteria.
Type:
Application
Filed:
April 16, 2003
Publication date:
January 13, 2005
Applicant:
University of Guelph
Inventors:
Chris Whitfield, Jeremy Yethon, David Heinrichs
Abstract: A method for embryogenesis and a method for plant regeneration are disclosed. Microspore-containing plant segment from donor plants are harvested and incubated under pre-treatment conditions to maintain microspore at a uninucleate cell cycle G1 phase. Pre-treatment conditions comprise cold water or an aqueous solution of about 0.2-about 1.0 mol/liter sugar alcohol, for example mannitol. Microspores are isolated from the plant segment and embryogenesis of microspores is induced in induction medium, thereby producing embryos. Green plants may be regenerated from the embryos produced. Arabinogalactan protein, auxin and ovary co-culture may be added to the induction medium to enhance embryogenesis from microspores.
Abstract: The invention provides a gene encoding a protein having ethanolaminephosphate cytidylyltransferase activity, and a promoter of an ethanolaminephosphate cytidylyltransferase gene. Nucleotide sequences according to SEQ ID NO:1 and SEQ ID NO:2 relate to the gene and the promoter, respectively.