Patents Assigned to Third Wave Technologies, Inc.
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Patent number: 6913881Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5? nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: November 15, 2000Date of Patent: July 5, 2005Assignee: Third Wave Technologies, Inc.Inventors: Brian D. Aizenstein, Eric B. Rasmussen, Jeff G. Hall, Poonam Agarwal, David Arco, Myrta W. Atiles, Deborah E. Burris, Monika de Arruda Indig, Scott M. Law, Andrea L. Mast, David J. Marshall, Carolyn W. Miller, Mary C. Oldenberg, James R. Prudent, Jennifer M. Schneiders, Mary Ann Brow, Victor Lyamichev
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Publication number: 20050130179Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.Type: ApplicationFiled: June 23, 2004Publication date: June 16, 2005Applicant: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Zbigniev Skrzypczynski, Hatim Allawi, Sarah Wayland, Tsetska Takova, Bruce Neri, James Prudent, Jeff Hall
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Publication number: 20050106596Abstract: The present invention relates to reactions conducted on or in solid surface materials, including liquid phase reactions within capillary tubes and arrays of capillary tubes or open end reaction microchambers. A wide array of biomolecular and combinatorial synthesis reactions may be used with the present invention. For example, the present invention provides methods for forming and cleaving nucleic acid cleavage structures on or in solid surface materials.Type: ApplicationFiled: August 27, 2004Publication date: May 19, 2005Applicant: Third Wave Technologies, Inc.Inventors: Zbigniev Skrzypczynski, Joerg Baier, Karl Nichols
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Patent number: 6875572Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: February 22, 2002Date of Patent: April 5, 2005Assignee: Third Wave Technologies, Inc.Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
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Patent number: 6872816Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.Type: GrantFiled: March 24, 1998Date of Patent: March 29, 2005Assignee: Third Wave Technologies, Inc.Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow, Robert W. Kwiatkowski, Stephanie H. Vavra
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Publication number: 20050048527Abstract: The present invention provides novel cleavage agents for the cleavage and modification of nucleic acid. The cleavage agents find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, an endonuclease activity is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: February 20, 2004Publication date: March 3, 2005Applicant: Third Wave Technologies, Inc.Inventors: Hatim Allawi, Michael Kaiser, Wu-Po Ma, Bruce Neri, Victor Lyamichev
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Publication number: 20040219576Abstract: The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers and modified phosphoramidates.Type: ApplicationFiled: January 6, 2004Publication date: November 4, 2004Applicant: Third Wave Technologies, Inc.Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
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Publication number: 20040203035Abstract: The present invention provides compositions and methods for the detection and characterization of mutations associated with non-syndromic hearing impairment. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of mutations in the Connexin 26, or gap junction beta 2, gene associated with non-syndromic hearing loss.Type: ApplicationFiled: January 9, 2004Publication date: October 14, 2004Applicant: Third Wave Technologies, Inc.Inventors: Andrea L. Mast, Erin Dorn, Robert J. Kwiatkowski, Molly Accola, Susan S. Wigdal
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Patent number: 6780585Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: October 2, 2000Date of Patent: August 24, 2004Assignee: Third Wave Technologies, Inc.Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, James E. Dahlberg, Lance Fors
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Patent number: 6780982Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.Type: GrantFiled: February 6, 2001Date of Patent: August 24, 2004Assignee: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Zbigniew Skrzpczynski, Hatim T. Allawi, Sarah R. Wayland, Tsetska Takova, Bruce P. Neri
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Patent number: 6759226Abstract: The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.Type: GrantFiled: May 24, 2000Date of Patent: July 6, 2004Assignee: Third Wave Technologies, Inc.Inventors: Wu-Po Ma, Victor I. Lyamichev, Michael W. Kaiser, Natalie E. Lyamicheva, Hatim Taysir Allawi, James J. Schaefer, Bruce P. Neri
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Publication number: 20040096874Abstract: The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. The present invention also provides cytochrome p450 genotyping methods and reagents for genotyping assays and a genomic DNA copy number assays.Type: ApplicationFiled: July 10, 2003Publication date: May 20, 2004Applicant: Third Wave Technologies, Inc.Inventors: Matt Neville, Monika de Arruda Indig, Feng Cao, Mary C. Oldenburg, James A. Koelbl, Brian D. Aizenstein, Keith Davey
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Patent number: 6709819Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).Type: GrantFiled: April 3, 2001Date of Patent: March 23, 2004Assignee: Third Wave Technologies, Inc.Inventors: Victor I. Lyamichev, Fang Dong, Mary Ann D. Brow, Lance Fors, Bruce P. Neri
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Patent number: 6709815Abstract: The present invention relates to methods and compns. for treating nucleic acids, and in particular, methods and compns. for the detection and characterization of nucleic acid sequences and sequence changes. The invention provides methods for examg. the conformations assumed by single strands of nucleic acid, forming the basis of novel methods of detection of specific nucleic acid sequences. The present invention contemplates use of novel detection methods for, among other uses, clinical diagnostic purposes, including but not limited to the detection and identification of pathogenic organisms. Examples are presented for the analysis of Mycobacterium tuberculosis and hepatitis C virus genes.Type: GrantFiled: July 18, 2000Date of Patent: March 23, 2004Assignee: Third Wave Technologies, Inc.Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg
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Patent number: 6706471Abstract: The present invention relates to methods for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5′ nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.Type: GrantFiled: June 14, 1999Date of Patent: March 16, 2004Assignee: Third Wave Technologies, Inc.Inventors: Mary Ann D. Brow, Jeff Steven Grotelueschen Hall, Victor Lyamichev, David Michael Olive, James Robert Prudent
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Patent number: 6692917Abstract: The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on dendrimers and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: August 27, 2001Date of Patent: February 17, 2004Assignee: Third Wave Technologies, INCInventors: Bruce P. Neri, Jeff G. Hall, Victor Lyamichev, Lloyd M. Smith
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Publication number: 20040014067Abstract: The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions.Type: ApplicationFiled: December 17, 2002Publication date: January 22, 2004Applicant: Third Wave Technologies, Inc.Inventors: Victor Lyamichev, Andrew Lukowiak, Nancy Jarvis, David Kurensky
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Patent number: 6673616Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.Type: GrantFiled: September 5, 2000Date of Patent: January 6, 2004Assignee: Third Wave Technologies, Inc.Inventors: James E. Dahlberg, Mary Ann D. Brow, Victor I. Lyamichev
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Publication number: 20030219784Abstract: The present invention relates to systems and methods for the nucleic acid based analysis of agricultural products. In particular, the present invention relates to the determination of wheat grades using nucleic acid analysis. The present invention further provides a lateral flow strip apparatus for use in nucleic acid detection assays. The present invention thus provides improved methods of grading commercially important grains (e.g., wheat).Type: ApplicationFiled: January 28, 2003Publication date: November 27, 2003Applicant: Third Wave Technologies, Inc.Inventors: Hon S. Ip, Witold A. Ziarno, Glen Donald
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Patent number: 6635463Abstract: The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.Type: GrantFiled: January 11, 2001Date of Patent: October 21, 2003Assignee: Third Wave Technologies, Inc.Inventors: Wu-Po Ma, Victor I. Lyamichev, Michael W. Kaiser, Natalie E. Lyamicheva, Hatim Taysir Allawi, James J. Schaefer, Bruce P. Neri