Patents Assigned to Trustees of Tufts College
  • Patent number: 10792328
    Abstract: A nanocomplex, of particle size 50 nm to 1000 nm, containing saporin and a lipid-like compound, in which saporin binds to the lipid-like compound via non-covalent interaction or covalent bonding. The lipid-like compound has a hydrophilic moiety, a hydrophobic moiety, and a linker joining the hydrophilic moiety and the hydrophobic moiety. The hydrophilic moiety is optionally charged and the hydrophobic moiety has 8 to 24 carbon atoms. Also disclosed is a pharmaceutical composition containing such a nanocomplex and a pharmaceutically acceptable carrier. The nanocomplex is useful in treating diseases, such as cancer.
    Type: Grant
    Filed: January 15, 2019
    Date of Patent: October 6, 2020
    Assignee: Trustees of Tufts College
    Inventors: Qiaobing Xu, Ming Wang
  • Patent number: 10758846
    Abstract: A microfluidic device includes a first layer of a porous material with pores having a first average pore size and a liquid-receiving area through which a liquid sample is received into the microfluidic device. A second layer of another porous material, with pores of a second average pore size, is stacked below the first layer and has a channel with a starting end positioned at least in part in an overlapping manner with the liquid-receiving area. The channel has a terminating end extending laterally at a predetermined wicking distance from the starting end. The first average pore size and the second average pore size cause a wicking effect in which at least some of the liquid sample flows along the channel at least a portion of the wicking distance between the starting end and the terminating end.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: September 1, 2020
    Assignee: Trustees of Tufts College
    Inventors: Charles R. Mace, Syrena C. Fernandes, Samuel B. Berry
  • Publication number: 20200263146
    Abstract: Provided herein are compositions and methods for vaccination and research applications. In particular, provided herein are non-neuroinvasive herpesviruses and alpha herpesviruses and uses thereof.
    Type: Application
    Filed: May 6, 2020
    Publication date: August 20, 2020
    Applicants: Northwestern University, Board of Regents of the University of Nebraska, Trustees of Tufts College (AKA Tufts University)
    Inventors: Gregory A. Smith, Patricia Jane Sollars, Gary Edward Pickard, Ekaterina E. Heldwein
  • Publication number: 20200263147
    Abstract: Provided herein are compositions and methods for vaccination and research applications. In particular, provided herein are non-neuroinvasive herpesviruses and alpha herpesviruses and uses thereof.
    Type: Application
    Filed: May 6, 2020
    Publication date: August 20, 2020
    Applicants: Northwestern University, Board of Regents of the University of Nebraska, Trustees of Tufts College (AKA Tufts University)
    Inventors: Gregory A. Smith, Patricia Jane Sollars, Gary Edward Pickard, Ekaterina E. Heldwein
  • Patent number: 10736943
    Abstract: A method was developed to prepare silk fibroin microspheres using lipid vesicles as templates to efficiently load therapeutic agents in active form for controlled release. The lipids are subsequently removed through the use of a dehydration agent, such as methanol or sodium chloride, resulting in ?-sheet structure dominant silk microsphere structures having about 2 ?m in diameter. The therapeutic agent can be entrapped in the silk microspheres and used in pharmaceutical formulations for controlled-release treatments.
    Type: Grant
    Filed: May 7, 2013
    Date of Patent: August 11, 2020
    Assignee: Trustees of Tufts College
    Inventors: David L. Kaplan, Xiaoqin Wang
  • Patent number: 10710026
    Abstract: A polymer fiber formed of statistical copolymers, each of which contains zwitterionic repeat units and hydrophobic repeat units, the zwitterionic repeat units constituting 20-75 wt % of the statistical copolymer and the hydrophobic repeat units being characterized in that a homopolymer formed thereof has a glass transition temperature above room temperature. Also disclosed is a fibrous membrane containing such polymer fibers in which greater than 90% of the polymer fibers are each independently rib bon-shaped fibers or wrinkly fibers. A method of preparing such a fibrous membrane is disclosed as well.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: July 14, 2020
    Assignee: Trustees of Tufts College
    Inventors: Ayse Asatekin, Peggy Cebe, Sefika Ozcan, Prity Bengani-Lutz
  • Patent number: 10712272
    Abstract: Methods and corresponding apparatus and systems for assessing cellular metabolic activity are disclosed. In one aspect, a cell can be illuminated with optical radiation in order to cause multi-photon excitation of at least one endogenous metabolic cofactor in that cell and cause the excited metabolic cofactor to emit fluorescent radiation. A detector can be used to detect the fluorescent radiation emitted by the excited endogenous metabolic cofactor. A computer processor can analyze the fluorescent radiation to derive the following parameters: (1) using a computer processor to analyze the intensity of the fluorescent radiation, (2) a fluorescence lifetime of at least one of the excited metabolic cofactor, (3) a parameter indicative of mitochondrial clustering in the cell. These parameters can be used to assess at least one metabolic process of the cell.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: July 14, 2020
    Assignee: TRUSTEES OF TUFTS COLLEGE
    Inventors: Irene Georgakoudi, Dimitra Pouli, Kyle P. Quinn, Zhiyi Liu
  • Publication number: 20200206269
    Abstract: Multi-step methods for the in vitro production of enucleated red blood cells and the enucleated red blood cells thus prepared are provided. Such enucleated red blood cells may express fusion proteins comprising an antigen binding protein which allows the red blood cell to bind a toxin or an antigen of a pathogen. Also described herein are methods for neutralizing a toxin or pathogen in a subject by administering enucleated red blood cells that express any of the fusion proteins provided herein.
    Type: Application
    Filed: August 22, 2018
    Publication date: July 2, 2020
    Applicants: Whitehead Institute for Biomedical Research, Trustees of Tufts College
    Inventors: Harvey Lodish, Nai-Jia Huang, Novalia Pishesha, Hidde L. Ploegh, Charles Shoemaker
  • Patent number: 10675362
    Abstract: A composition, as well as methods using the composition, for detection or quantification of a molecule at a singlet state (e.g., singlet oxygen). The composition includes one or more nanoparticles, and the nanoparticle has an energy donor, an energy acceptor associated with the energy donor, and an energy transfer mechanism between the energy donor and the energy acceptor.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: June 9, 2020
    Assignee: Trustees of Tufts College
    Inventors: Samuel W. Thomas, III, Fanny Frausto, Jingjing Zhang
  • Publication number: 20200172536
    Abstract: Identified compounds demonstrate protein kinase inhibitory activity. More specifically, the compounds having the structures below (I) are demonstrated to inhibit receptor interacting kinase 2 (RIPK2) and/or Activin-like kinase 2 (ALK2). Compounds that are either dual RIPK2/ALK2 inhibitors or that preferentially inhibit RIPK2 or ALK2 could provide therapeutic benefit.
    Type: Application
    Filed: May 15, 2018
    Publication date: June 4, 2020
    Applicants: UNIVERSITY OF HOUSTON SYSTEM, TRUSTEES OF TUFTS COLLEGE
    Inventors: Gregory Cuny, Sameer Nikhar, Alexei Degterev
  • Patent number: 10668140
    Abstract: Described are non-toxigenic Clostridium difficile strains and spores. Also described are vaccines comprising the Clostridium difficile spores. Further described are methods of preventing or treating a Clostridium difficile infection in a subject in need thereof.
    Type: Grant
    Filed: February 26, 2018
    Date of Patent: June 2, 2020
    Assignees: University of South Floirida, Trustees of Tufts College
    Inventors: Xingmin Sun, Abraham Sonenshein
  • Patent number: 10662409
    Abstract: In some embodiments, the present invention provides methods including the steps of providing one or more human somatic cells, causing transient increased expression of OCT4, KLF4, SOX2, and cMYC in the somatic cells forming modified somatic cells, providing a plurality of inactivated embryonic fibroblasts, associating the modified somatic cells with the inactivated embryonic fibroblasts in a culture media comprising 20% KO DMEM xeno-free serum replacement and at least 15 ng/ml recombinant bFGF to form human induced neural stem cells.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: May 26, 2020
    Assignee: Trustees of Tufts College
    Inventors: Dana Cairns, David L. Kaplan
  • Patent number: 10653786
    Abstract: Provided herein relates to compositions and methods for lubrication of a surface. The surface amenable to the compositions and methods described herein can be a non-biological surface, a biological surface, or a combination thereof. In some embodiments, the composition comprising a phospholipid-coated silk microsphere can be used for lubrication of a surface. In some embodiments, the composition comprising a phospholipid-coated silk microsphere can be used for joint lubrication, e.g., for treatment of joint disorders such as arthritis.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: May 19, 2020
    Assignees: TRUSTEES OF TUFTS COLLEGE, Tulane Educational Fund
    Inventors: David L. Kaplan, Xiaoqin Wang, Vijay John, Noshir Pesika, Rubo Zheng
  • Patent number: 10647964
    Abstract: Provided herein are compositions and methods for vaccination and research applications. In particular, provided herein are non-neuroinvasive herpesviruses and alpha herpesviruses and uses thereof.
    Type: Grant
    Filed: March 4, 2016
    Date of Patent: May 12, 2020
    Assignees: Northwestern University, Board of Regents of the University of Nebraska, Trustees of Tufts College
    Inventors: Gregory A. Smith, Patricia Jane Sollars, Gary Edward Pickard, Ekaterina E. Heldwein
  • Publication number: 20200123498
    Abstract: The disclosure provides methods and compositions for affecting the development of antigen presenting cell (APC, e.g., a macrophage or dendritic cell). The methods include maturing an APC, promoting anti-inflammatory phenotype, promoting development of a T regulatory cell (Treg) from a naive T cell. The methods generally include exposing an APC to a tryptophan derived microbiota metabolite (TDMM), such as an anti-inflammatory or pro-mucosal TDMM, and permitting the APC to mature. In some embodiments, the conditioned APC is exposed to a naive T cell to further promote development of a T regulatory cell (Treg). In some embodiments, the TDMM is selected from the group consisting of indole, indole-3-acetate, 5-hydroxyindole, and indole-3-pyruvate.
    Type: Application
    Filed: March 17, 2017
    Publication date: April 23, 2020
    Applicants: THE TEXAS A&M UNIVERSITY SYSTEM, TRUSTEES OF TUFTS COLLEGE
    Inventors: Robert C. Alaniz, Arul Jayaraman, Kyongbum Lee
  • Patent number: 10620214
    Abstract: As described below, the invention provides methods for localizing and quantifying the extent to which a molecule penetrates a cell.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: April 14, 2020
    Assignee: TRUSTEES OF TUFTS COLLEGE
    Inventors: Joshua Kritzer, Leila Peraro
  • Patent number: 10603637
    Abstract: A two-layer photo-responsive membrane including a polymer layer and a support layer, the polymer layer being disposed on a surface of the support layer. The polymer layer is formed of a graft copolymer that contains a hydrophobic backbone and multiple side chains, the side chains each consisting of repeat units that switch between a hydrophobic form and a hydrophilic form upon exposure to a light of a specific wavelength. The polymer layer has a molecular weight cut-off of 3,000 to 250,000 Daltons and a thickness of 50 nm to 10 ?m; and the support layer has a molecular weight cut-off of 50 to 250,000 Daltons. Also disclosed is a method of preparing this two-layer photo-responsive membrane.
    Type: Grant
    Filed: June 1, 2016
    Date of Patent: March 31, 2020
    Assignee: Trustees of Tufts College
    Inventors: Ayse Asatekin Alexiou, Samuel Thomas, Papatya Kaner, Xiaoran Hu
  • Patent number: 10583090
    Abstract: The present invention provided for a novel process of forming silk fibroin gels, and controlling the rate of ?-sheet formation and resulting hydrogelation kinetics, by vortex treatment of silk fibroin solution. In addition, the vortex treatment of the present invention provides a silk fibroin gel that may be reversibly shear-thinned, enabling the use of these approach for precise control of silk self-assembly, both spatially and temporally. Active agents, including biological materials, viable cells or therapeutic agents, can be encapsulated in the hydrogels formed from the processes, and be used as delivery vehicles. Hence, the present invention provide for methods for silk fibroin gelation that are useful for biotechnological applications such as encapsulation and delivery of active agents, cells, and bioactive molecules.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: March 10, 2020
    Assignee: Trustees of Tufts College
    Inventors: David L. Kaplan, Tuna Yucel
  • Patent number: 10548981
    Abstract: The present invention provides for novel sustained release silk-based delivery systems. The invention further provides methods for producing such formulations. In general, a silk fibroin solution is combined with a therapeutic agent to form a silk fibroin article. The article is then treated in such a way as to alter its conformation. The change in conformation increases its crystallinity or liquid crystallinity, thus controlling the release of a therapeutic agent from the formulation. This can be accomplished as single material carriers or in a layer-by-layer fashion to load different therapeutic agents or different concentrations of these agents in each layer.
    Type: Grant
    Filed: December 3, 2013
    Date of Patent: February 4, 2020
    Assignees: EIDGENOSSISCHES TECHNISCHE HOCHSCHULE (THE SWISS FEDERAL INSTITUTE OF TECHNOLOGY), TRUSTEES OF TUFTS COLLEGE
    Inventors: David L. Kaplan, Lorenz Meinel
  • Publication number: 20200032326
    Abstract: Described herein are ultrasensitive methods to detect the presence and/or measure the levels of short target nucleic acids, such as microRNAs, in a sample. Such a method can involve the use of a capture probe and a detection probe, each of which is complementary to a segment of the short target nucleic acid. The capture probe and a detection probe may be hybridized with the target nucleic acid in the sample and the complex thus formed can be detected, for example, by a single molecular array assay.
    Type: Application
    Filed: May 29, 2019
    Publication date: January 30, 2020
    Applicant: Trustees of Tufts College
    Inventors: David R. Walt, Limor Cohen, Mark Hartman