Abstract: The present invention concerns human hepatocyte cell line cultures and their use in bioartificial liver (BAL) systems. These systems are used to treat subjects suffering from liver failure to temporarily compensate for loss of hepatocellular function and generally comprise a bioreactor loaded with functional liver cells. Until now, it has been problematic to acquire cells with a broad spectrum metabolic functionality, resembling that of freshly isolated human hepatocytes, to the extent that they are in fact suitable for successful clinical BAL application The present inventors have managed to develop human hepatocyte cell line cultures that display broad-spectrum metabolic functionality such as to render them particularly suitable for effective clinical BAL application.

Abstract: The present invention relates to a method of treating or preventing hyperproliferative disease in a body tissue of a subject, comprising the steps of administering to a subject in need thereof a therapeutically effective amount of an agent that induces double strand breaks in the DNA of the hyperproliferative cells of said body tissue; and subjecting the hyperproliferative cells of said body tissue prior to, simultaneously with or subsequent to step a) to hyperthermia to thereby induce in said cells the degradation, inhibition and/or inactivation of BRCA2.

Abstract: A mammography-apparatus for detecting malignant cells in a breast comprising an X-ray source, an X-ray detector and a paddle for pressing the breast against said X-ray detector, wherein the mammography-apparatus further comprises a non-focussed laser-beam source for pulsed laser-light, which during use is aimed at the breast, and said apparatus comprises at least one non-contact ultrasound detector for contact-free detection of an ultrasound originating from said breast so as to screen the occurrence or non-occurrence of said malignant cells.

Abstract: The invention relates to antioxidants or radical scavengers and their reaction products. The invention provides compounds and compositions for use in methods for scavenging radicals or for modulating the immune response comprising urocanic acid or salts, derivatives, functional equivalents and analogues thereof.

Abstract: A composition comprising an oral rehydration solution and Yacón Syrop, is described. Such composition may be used as a medicament, in particular for preventing or treating one or more symptoms of a hangover, such as a headache, nausea and stomach ache.

Abstract: A process of producing an organic compound and/or an intermediary compound as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting the culture to light, wherein the cell is capable of expressing a nucleic acid molecule, wherein the expression of the nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into the organic compound and/or into the intermediary compound and wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.

Abstract: Described are methods and devices for determining a concentration of oxygen in a compartment comprising exciting protoporphyrin IX.

Abstract: The present disclosure pertains to a process for preparing an oxime in which a carbonyl compound is reacted in the liquid phase with NH3 and H2O2 in the presence of a catalyst to form the corresponding oxime, wherein the catalyst comprises a catalytic component selected from the oxides of metals of group 5 and group 6. The use of a niobia catalyst is particularly preferred. The process according to the disclosure is suitable for the manufacture of numerous oximes, in particular cyclohexanone oxime.

Abstract: Disclosed are antagonists designed to inhibit or block expression of a mammalian complement such as complement component 6 (C6). The invention has a wide range of uses including use in the preparation of a medicament for the enhancement of nerve regeneration following acute or chronic nerve damage in a mammal. Additional applications include use in the treatment of multiple sclerosis either alone or in combination with another drug.

Abstract: The invention provides a method for producing a stabilized cell of interest, said method comprising providing a stem cell and/or a precursor cell of said cell of interest with a nucleic acid sequence which, when present in said cell of interest, is capable of stabilizing said cell of interest, providing a non-human animal with said stem cell and/or precursor cell, allowing generation of said cell of interest in said animal, and obtaining said cell of interest. Said animal is preferably provided with a human stem cell and/or human precursor cell, allowing production of a human cell line.

Abstract: The invention relates to a method for imaging an object, comprising the illumination of the object, and the detection of light coming from an area where the object is located and, based on the detected light, forming the image, with the illumination of the object taking place by distinguishing image elements in an imaging plane or space, and by separately illuminating the object parts corresponding with these image elements, while simultaneously registering the applied illumination dose of these object parts, and wherein the image is subsequently constructed by assembling the image elements corresponding with the illuminated object parts, subject to the light output and illumination doses pertaining to those image elements, wherein the illumination dose of each separate object part depends at least on a light output and/or illumination dose of another object part or several other object parts.

Abstract: The invention provides means and methods for providing a cell with a spontaneous electrical activity and means and methods for increasing the depolarization rate of a cell having a spontaneous electrical activity. Means and methods are provided comprising: providing a cell with a compound capable of providing and/or increasing a pacemaker current If, and diminishing electrical coupling between said cell and surrounding cells and/or reducing the inward rectifier current IK1 of said cell, and or increasing the availability of INa at depolarized potentials, preferably using overexpression of additional sodium channels.

Abstract: Described are methods for determining a concentration of a substance in a compartment comprising exciting an endogenous compound, or a functional part, derivative, analogue or precursor thereof, measuring the lifetime of the luminescence and/or transient absorption exhibited by the compound, functional part, derivative, analogue and/or precursor, and correlating the lifetime with the concentration of the substance.

Abstract: A pathogenic factor in plasma as an improved therapy for Fabry disease.

Abstract: A manipulator for an instrument for minimally invasive surgery, having at a proximal end a handle for operating the instrument, and wherein the instrument is removably placed at a distal end, and wherein a parallelogram construction is provided between the proximal end and the distal end for guaranteeing an unambiguous positional relationship between the handle and the instrument, wherein the parallelogram construction is coupled with a system of bars for controlling the position of the parallelogram construction, the bars of the system of bars being connected to the parallelogram construction as well as to each other by means of cardan joints.

Abstract: The invention provides a method for influencing the stability of an antibody producing cell, comprising directly or indirectly influencing the amount of BCL6 and/or Blimp 1 expression product within said antibody producing cell. Stable antibody producing cells and cell lines are also provided, as well as methods for producing antibodies using such cells and/or cell lines.

Abstract: The present invention is in the field of Fabry disease and concerns a pathogenic factor allowing diagnosis of Fabry disease. In particular lyso-ceramide trihexosamide (lyso-CTH) has been found to function as a diagnostic marker for Fabry disease.

Abstract: The invention discloses a method for detecting an activity of an endoglycosidase. The method includes providing the endoglycosidase with a substrate of the endoglycosidase and detecting cleavage of the substrate. The method further includes at least partly inhibiting the transglycosidase activity of the endoglycosidase. The transglycosidase activity may be inhibited by chemically modifying the substrate such that transglycosylation of the substrate by the endoglycosidase is at least partly inhibited while the endoglycosidase is still capable of cleaving the substrate. In one embodiment, the substrate comprises an oligosaccharide chain. Compounds and kits suitable for use in a method of the invention are also disclosed. Methods involving competitive inhibitors are also disclosed as are methods for the synthesis of glycosylated substrates involving the transglycosidase activity of an endoglycosidase.

Abstract: The present invention describes a novel cross-linker, a method for preparing one or more cross-linked biomolecules, biomolecular complexes of two or more biomolecules, a method for preparing cross-linked fragments from such cross-linked biomolecules and/or biomolecular complexes, a method for cleavage and reduction of such cross-linked biomolecules and/or biomolecular complexes, a method for identifying cross-links in such cross-linked biomolecules and/or biomolecular complexes, as well as a method for determining relative amounts of cross-links in a biomolecule or biomolecular complex in two or more samples.

Abstract: The invention is directed to a process for the production of certain phosphorous, namely urea, thio-urea and sulphonamide phosphorous compounds. The present invention provides a process for the production of phosphorous compounds which process allows an easy and effective separation of the reaction products from impurities by applying a solid alkaline ion-exchange resin.