Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
Type:
Grant
Filed:
September 21, 2021
Date of Patent:
April 30, 2024
Assignees:
The Regents of the University of California, University of Vienna
Inventors:
Jennifer A. Doudna, Martin Jinek, Krzysztof Chylinski, Emmanuelle Charpentier
Abstract: Disclosed herein are compositions and methods for the treatment of otic disorders with immunomodulating agents and auris pressure modulators. In these methods, the auris compositions and formulations are administered locally to an individual afflicted with an otic disorder, through direct application of the immunomodulating and/or auris pressure modulating compositions and formulations onto the auris media and/or auris interna target areas, or via perfusion into the auris media and/or auris interna structures.
Type:
Grant
Filed:
August 18, 2021
Date of Patent:
April 30, 2024
Assignees:
DOMPÉ FARMACEUTICI S.P.A., THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors:
Jay Lichter, Andrew M. Trammel, Fabrice Piu, Qiang Ye, Michael Christopher Scaife, Benedikt Vollrath, Sergio G. Duron, Luis A. Dellamary, Carl Lebel, Jeffrey P. Harris
Abstract: Provided are compositions and methods that include one or more of: (1) a Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the effector protein, and/or a modified host cell comprising the effector protein (and/or a nucleic acid encoding the same); (2) a CRISPR/Cas guide RNA that binds to and provides sequence specificity to the Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the CRISPR/Cas guide RNA, and/or a modified host cell comprising the CRISPR/Cas guide RNA (and/or a nucleic acid encoding the same); and (3) a CRISPR/Cas transactivating noncoding RNA (trancRNA), a nucleic acid encoding the CRISPR/Cas trancRNA, and/or a modified host cell comprising the CRISPR/Cas trancRNA (and/or a nucleic acid encoding the same).
Type:
Grant
Filed:
October 31, 2018
Date of Patent:
April 30, 2024
Assignee:
The Regents of the University of California
Inventors:
Jennifer A. Doudna, David Burstein, Janice S. Chen, Lucas B. Harrington, David Paez-Espino, Jillian F. Banfield
Abstract: Embodiments of the presently disclosed technology provide a synergistic combination of a conjugated open-shell donor-acceptor polymer with a carbon-based compound (e.g., reduced graphene oxide) to produce a composite electrode material which demonstrates state-of-the-art capacitance and potential window, with excellent kinetics and cycle life. The conjugated open-shell donor-acceptor polymer may comprise a plurality of alternating electron-rich monomers (i.e., donors) and electron-deficient monomers (i.e., acceptors) bonded together via a conjugated backbone. The conjugated backbone may comprise a connection of n-orbitals of the plurality of monomers in alternating single and double bonds that facilitates unpaired electron delocalization—thereby stabilizing charge for the polymer. The carbon-based compound of the composite electrode material may provide porous, conductive scaffolds for the composite electrode material, resulting in electrodes scalable to microns-thick films with fast kinetics.
Type:
Grant
Filed:
June 14, 2022
Date of Patent:
April 30, 2024
Assignees:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE UNIVERSITY OF SOUTHERN MISSISSIPPI
Abstract: Optoelectronic devices, such as photovoltaic devices, comprising a low band gap, solution processable diketopyrrolopyrrole or dithioketopyrrolopyrrole chromophore core or cores are disclosed. Also disclosed are methods of fabricating such optoelectronic devices.
Type:
Grant
Filed:
January 29, 2018
Date of Patent:
April 30, 2024
Assignee:
The Regents of the University of California
Inventors:
Thuc-Quyen Nguyen, Arnold Bernarte Tamayo, Bright Walker, Tyler Kent, Chunki Kim, Mananya Tantiwiwat
Abstract: The technology described in this document can be used to implement beam steering in optical systems and photonic devices, to provide a nonmechanical beam steering system for projecting optical energy and controlling the direction of the optical energy using a collection of devices and components that are fixed in position to selectively direct light from an array of different optical emitters at different locations.
Type:
Grant
Filed:
September 29, 2020
Date of Patent:
April 30, 2024
Assignee:
The Regents of the University of California
Inventors:
Andrew Grieco, Abdelkrim El Amili, Yeshaiahu Fainman, Stephen Pappert
Abstract: A method may include embedding, in a hidden layer and/or an output layer of a first machine learning model, a first digital watermark. The first digital watermark may correspond to input samples altering the low probabilistic regions of an activation map associated with the hidden layer of the first machine learning model. Alternatively, the first digital watermark may correspond to input samples rarely encountered by the first machine learning model. The first digital watermark may be embedded in the first machine learning model by at least training, based on training data including the input samples, the first machine learning model. A second machine learning model may be determined to be a duplicate of the first machine learning model based on a comparison of the first digital watermark embedded in the first machine learning model and a second digital watermark extracted from the second machine learning model.
Type:
Grant
Filed:
March 21, 2019
Date of Patent:
April 30, 2024
Assignee:
The Regents of the University of California
Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
Type:
Grant
Filed:
November 13, 2020
Date of Patent:
April 30, 2024
Assignee:
The Regents of the University of California
Inventors:
Mark A. Akeson, David W. Deamer, William B. Dunbar, Roger Jinteh Arrigo Chen, Noah A. Wilson
Abstract: The present disclosure provides methods and related compositions that incorporate a molecularly targeted approach via the integrin subtype ?v?6 using Peptide Receptor Radionuclide Therapy (PRRT) and a theranostic approach.
Type:
Application
Filed:
December 3, 2021
Publication date:
April 25, 2024
Applicant:
The Regents of the University of California
Abstract: The present disclosure provides a composition comprising extracellular vesicles (EVs) derived from induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs). The present EVs possess immunoregulatory functions that are distinct from those of EVs derived from mesenchymal stem cells. The present EVs can be used to treat or modulate immune responses in diseases such as cancer or autoimmune diseases.
Type:
Application
Filed:
March 4, 2022
Publication date:
April 25, 2024
Applicant:
The Regents of the University of California
Inventors:
Jana ZARUBOVA, Mohammad Mahdi HASANI-SADRABADI, Song Li
Abstract: Methods are described for preventing or treating skeletal diseases such as period paralysis by administering to a subject a Kv7 channel opener.
Type:
Application
Filed:
October 23, 2023
Publication date:
April 25, 2024
Applicant:
The Regents Of the University of California
Abstract: A deep learning-based digital staining method and system are disclosed that enables the creation of digitally/virtually-stained microscopic images from label or stain-free samples based on autofluorescence images acquired using a fluorescent microscope. The system and method have particular applicability for the creation of digitally/virtually-stained whole slide images (WSIs) of unlabeled/unstained tissue samples that are analyzes by a histopathologist. The methods bypass the standard histochemical staining process, saving time and cost. This method is based on deep learning, and uses, in one embodiment, a convolutional neural network trained using a generative adversarial network model to transform fluorescence images of an unlabeled sample into an image that is equivalent to the brightfield image of the chemically stained-version of the same sample.
Type:
Application
Filed:
December 18, 2023
Publication date:
April 25, 2024
Applicant:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: Broadly applicable and stereoselective formation of glycosidic linkage remains challenging yet of critical importance in giycoscience. By developing an SN2 glycosylation, this work advances a general solution to this challenge via stereoinversion at the anomeric position of glycosyl ester donors. This SN2 process is enabled by a basic directing-group in the leaving-group, which is activated by a cationic gold catalyst or any other electrophilic reagent. Unlike all the reported directing group approaches, this strategy is applicable to any glycosyl donors—a long sought-after yet unmet goal in carbohydrate chemistry; moreover, the basic directing-group upon glycosylation is lost as part of the leaving-group and hence traceless in the glycoside products, therefore avoiding potential complications in downstream transformations. Highly selective construction of glycosidic bonds including challenging 1,2-cis glycosidic bonds is achieved in excellent yields.
Type:
Application
Filed:
January 28, 2022
Publication date:
April 25, 2024
Applicant:
The Regents of the University of California
Abstract: Systems and methods for implementation of a disposable miniaturized implant for treatment of Post-Operative Ileums (POI), a miniaturized implant for treating chronic GI dysmotility (e.g., dysphagia, gastroesophageal reflux disease (GERD), nausea, functional dyspepsia, blockage of transit, and gastroparesis, inflammatory bowel disease) and obesity, by providing electrical stimulation to the part of bowel going through surgery to expedite the healing process while recording the smooth muscle activities simultaneously, or providing stimulation on a treatment location of the GI tract or the branch of the vagus nerve. Systems and methods are also provided for non-invasive, transcutaneous stimulation of anatomy within the abdomen of the patient.
Type:
Application
Filed:
October 31, 2023
Publication date:
April 25, 2024
Applicant:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: The present invention relates to diarylhydantoin compounds, including diarylthiohydantoins, and methods for synthesizing them and using them in the treatment of hormone refractory prostate cancer.
Type:
Application
Filed:
June 13, 2023
Publication date:
April 25, 2024
Applicant:
The Regents of the University of California
Inventors:
Charles L. Sawyers, Michael Jung, Charlie D. Chen, Samedy Ouk, Derek Welsbie, Chris Tran, John Wongvipat, Dongwon Yoo
Abstract: A cabin air filtration system and method for regenerating a cabin air filtration system are disclosed. The filtration system includes a frame, and a renewable sorption layer within the frame, the renewable sorption layer configured to clean air from one or more of outside of an automobile and inside an automobile. The cabin air filtration system for an automobile can include a first layer, the first layer configured to particulates, and a second layer, the second layer configured to remove volatile compounds and gases. The method includes placing a renewable sorption layer within a frame in an oven, the renewable sorption layer configured to filter air from one or more of outside of the automobile and inside the automobile; creating a vacuum within the oven; and heating a source of water within the oven to perform a steam regeneration treatment on the renewable sorption layer.
Type:
Application
Filed:
October 19, 2023
Publication date:
April 25, 2024
Applicant:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Abstract: An epoxy resin-based embedding media doped with a non-conductive dopant to a predetermined w/w % such that the media is non-charging at 1.8 keV. A preferred dopant is polyethylene glycol at a molecular weight of at least 3350, and having a predetermined w/w % is at least 2% and up to 20%, most preferably from 2% to 10%. Another preferred dopant is polyethylene glycol at a molecular weight of 7000-8000 and a predetermined w/w % of up to ˜40% and more preferably of up to ˜30%.
Type:
Grant
Filed:
April 23, 2021
Date of Patent:
April 23, 2024
Assignee:
The Regents of the University of California
Inventors:
Thomas Deerinck, Mark Ellisman, Steven Peltier
Abstract: Methods, systems, and compositions are provided for implanting an implantable device into a biological tissue (e.g., muscle, brain). A subject implantable device includes: (i) a biocompatible substrate, (ii) a conduit (e.g., an electrode, a waveguide) that is disposed on the biocompatible substrate, and (iii) an engagement feature (e.g., a loop) for reversible engagement with an insertion needle. The biocompatible substrate can be flexible (e.g., can include polyimide). The implantable device is implanted using an insertion needle that includes an engagement feature corresponding to the engagement feature of the implantable device. To implant, an implantable device is reversibly engaged with an insertion needle, the device-loaded insertion needle is inserted into a biological tissue (e.g., to a desired depth), and the insertion needle is retracted, thereby disengaging the implantable device from the insertion needle and allowing the implantable device to remain implanted in the biological tissue.
Type:
Grant
Filed:
April 25, 2023
Date of Patent:
April 23, 2024
Assignee:
The Regents of the University of California
Inventors:
Timothy L. Hanson, Michel M. Maharbiz, Philip N. Sabes
Abstract: The disclosure provides stable antimicrobial (e.g., antibacterial or antifungal or both) peptides (SAMPs) that may be used in methods of preventing or treating a bacterial disease (e.g., a Liberibacter disease, such as citrus greening disease (also called Huanglongbing (HLB)) or potato Zebra Chip disease, and other bacterial diseases such as those caused by Agrobacterium tumefaciens (also known as Rhizobium radiobacter) and Pseudomonas syringae) in plants (e.g., citrus plants or potato plants). SAMPs disclosed herein may be heat stable, as well as stable in plant extracts and/or in plant lysates (e.g., citrus lysates).
Type:
Grant
Filed:
October 23, 2018
Date of Patent:
April 23, 2024
Assignee:
The Regents of The University of California
Abstract: Aspects of quadrature phase shift keying for quadrature amplitude modulation are described. In some examples, quadrature phase shift keying signals are generated using quadrature phase shift keying modulators. The quadrature phase shift keying signals are combined in a combiner circuit to generate a quadrature amplitude modulation signal for wireless transmission.
Type:
Grant
Filed:
June 17, 2020
Date of Patent:
April 23, 2024
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA