Abstract: Novel bacterial preparations containing one or more isolated and purified strain of a microorganism which produces one or more RTX toxins, and which strain has at least one RTX toxin which is substantially cell-associated. Methods of preparing the bacterial preparations and their use as vaccines and to produce antibodies for passive immunization are described.

Abstract: A purified DNA molecule comprises a DNA sequence of approximately 15.1 kb coding for normal or mutant RYR1 protein having a molecular weight of approximately 564,740 daltons. The DNA molecule has an endonuclease restriction map of FIG. 1 and a sequence of FIG. 2.

Abstract: Novel nucleic acid molecules encoding proteins involved in the synthesis and assembly of O-antigen in P. aeruginosa; and novel proteins encoded by the nucleic acid molecules are described. Methods are disclosed for detecting P. aeruginosa in a sample by determining the presence of the proteins or a nucleic acid molecule encoding the proteins in the sample.

Abstract: A feed additive for dairy cattle is described comprising DHA and inhibitors of microbial degradation of DHA in the rumen of the dairy cattle. A method of producing milk in dairy cattle which is enriched for DHA is also described comprising feeding the dairy cattle a feed containing the feed additive of the invention for a period of at least about 14 day, and milking the dairy cattle to obtain milk enriched for DHA.

Abstract: The present invention provides a method and apparatus for nondestructive, in situ measuring thicknesses of layers on substrates. The method and device uses a probe including a radioactive source in a source holder and a photodetector mounted behind the source for detection of backscattered photons. In one aspect the method is used to measure the thickness of paint deposited onto metal substrates. The source holder and photodetector array forms a cylindrically symmetric probe for producing an axially symmetric beam of primary photons. A source containing radioactive .sup.109 Cd producing high energy photons of energy 22 and 25 keV is spaced from the painted surface so the photons impinge on the painted substrate. The intensity of photons backscattered by Compton scattering in the paint layer is proportional to the mass density of the paint to give a direct measurement of the paint thickness. The photons penetrating through to the substrate are absorbed within the substrate.

Abstract: A method for identifying non-sex specific and sex specific molecules associated with animal cell membranes is described. The method involves preparing a cell membrane fraction from adult, fetal, or embryonic animal cells; treating the cell membrane fraction with one or more substances which bind to non-sex specific molecules in the cell membrane fraction to form conjugates between the non-sex specific molecules and the substances; separating the material in the cell membrane fraction which does not bind to the substances to obtain a subfraction containing sex specific molecules; optionally, removing the substances in the conjugates to obtain a subfraction containing non-sex specific molecules; and, isolating the sex specific molecules and optionally non-sex specific molecules in the subfractions. Sex specific and non-sex specific molecules and methods of using the sex specific and non-sex specific molecules for sexing are also described.

Abstract: The S-locus of Brassica contains the genetic information that encodes for self-incompatibility. In its first aspect, it is directed to an isolated gene, the SRK-910 gene, that segregates with the self-incompatibility phenotype. In its second aspect, the present invention is directed to an isolated cDNA that corresponds to the isolated gene and that has 2749 nucleotides.The isolated cDNA of the present invention encodes for a protein, i.e., the S-locus receptor kinase-910 protein ("the SRK-910 protein") which is also a part of the present invention. The SRK-910 protein, has 858 amino acids and is encoded for by the first 2575 nucleotides of the isolated cDNA of the present invention.The present invention is also directed to an oligonucleotide probe that is capable of distinguishing the SRK-910 gene from partially homologous genes at the S-locus that encode for the S-locus glycoproteins.

Abstract: The present invention provides a method and apparatus for in situ measuring thicknesses of ice buildup on airfoil. The method and device uses a probe including a high energy radioactive gamma ray source .sup.241 Am producing 60 keV photons which penetrate through the airfoil substrate and a photodetector mounted behind the source for detection of backscattered photons. The probe is mounted on the interior of the airfoil and secondary radiation is backscattered within the ice layer and back through the airfoil substrate to the photodetector. The shape and density of the source holder in addition to the geometrical arrangement of the source and detector with respect to the airfoil substrate are used to block photons backscattered in the airfoil substrate thereby favoring scattering in the ice layer over that in the aluminum.

Abstract: Provided is a method and apparatus for detecting electroinactive ions, and electroinactive ions solvated by small molecules including water. The method and apparatus utilizes a microporous ion exchange material such as a zeolite containing initially an electroactive species contained within the pores. The method operates on the principle that when charged species present in solution can access the pores of the microporous material, an ion-exchange reaction will occur whereupon the electroactive species will exit the microporous material, being replaced by the charged species. The electroactive species, upon exiting the microporous material, undergoes electrochemical reduction or oxidation at an electrode having a sufficiently high potential applied thereto. The method and detector disclosed herein is size selective since for example zeolites with a pore size comparable to that of the solvated ion to be detected but smaller than other electrolyte components can be utilized.

Abstract: The present invention relates to computational methods for designing chemical structures sharing common useful, functional properties based on specific combinations of steric configuration and binding affinity. More particularly the present invention provides a method for producing computer-simulated receptors which functionally mimic biological receptors. The simulated receptors are designed to exhibit optimized selective affinity for known target molecules. Chemical structures are then generated and evolved to exhibit selective affinity for the simulated receptors.

Abstract: An improved route is provided for the production of seeds capable of forming F.sub.1 hybrid plants of a crop which is capable of undergoing cross-pollination while utilizing self-incompatibility. A substantially randomly mixed population of parent plants is utilized that are substantially incapable of undergoing self-pollination under conventional growing conditions wherein the inability to undergo self-pollination is attributable in each of the parent plants to at least one heterozygous dominant genetic determinant for sporophytic self-incompatibility that is different in each parent. All plants are capable of serving (1) as seed parents which following cross-pollination with pollen from the other parent plants bear seeds capable of forming F.sub.1 hybrid plants of a predetermined cultivar, and (2) as pollen parents for other parent plants. The non-selective simultaneous harvest by conventional means from both parent plants of seed capable of forming a single predetermined cultivar of F.sub.

Abstract: A purified DNA molecule encoding a glycoprotease from Pasteurella haemolytica is disclosed. The DNA comprises a sequence of approximately 975 base pairs coding for a glycoprotease having a molecular weight of approximately 35.2 kD. The glycoprotease is specific for cleaving O-glycosylated carbohydrate portions from O-glycoproteins. The glycoprotease has a major cleavage site in glycophorin A between Arg31 and Asp32.

Abstract: There is disclosed a new boron-containing compounds such as bis-(pentafluorophenyl)borane, (C.sub.6 F.sub.5).sub.2 BH and related compounds and a method for producing the compounds. The new borane is suitable for use as a hydroboration reagent for the rapid hydroboration of alkenes and alkynes. The borane is also suitable for use as a hydroboration reagent to produce useful boron reagents that can be used in Ziegler-Natta homogeneous olefin polymerization catalyst systems. Specifically, the borane reagent can be used to produce Cp.sub.2 Zr{.eta..sup.3 -CH(C.sub.6 H.sub.5)[(.mu.-H)B(C.sub.6 F.sub.5).sub.2 ]}, which exhibits an efficacy for polymerization of ethylene.

Abstract: A serum-free vaccine effective against pneumonic pasteurellosis in cattle comprising a non-toxic leukotoxin specific for ruminant leukocytes is disclosed. The leukotoxin is prepared in a serum-free medium from a culture of Pasteurella haemolytica. The produced leukotoxin is harvested from the culture medium upon detecting a certain stage during the logarithmic phase of the cell growth to obtain the optimum concentration of produced cytotoxin in the serum-free medium. Cattle may be treated with the vaccine to develop anti-leukotoxic immunity to pneumonic pasterellosis.

Abstract: A purified DNA molecule comprises a DNA sequence of approximately 15.1 kb coding for normal or mutant RYR1 protein having a molecular weight of approximately 564,740 daltons. The DNA molecule has an endonuclease restriction map of FIG. 1 and a sequence of FIG. 2.

Abstract: A radioactive source holder for use with a NaI (TI) spectrometer for obtaining very low dose estimates in bone minerals in vivo, comprising a molybdenum tube, a gold insert within the tube, a blind hole within the insert for receiving the radioactive source, and an inner tube inserted into the blind hole for directing radiation from the source in a narrow cone, whereby the directing of radiation in the narrow cone minimizes spectrometer response to radiation which has been scattered by soft tissue overlying a bone.

Abstract: A process for inducing desiccation tolerance in an in vitro formed plant embryo to enable production of viable artificial seeds is disclosed. The process comprises culturing in vitro plant embryos, stimulating the embryos to proceed through globular and a heart shaped stage into an elongate-torpedo shaped stage and early cotyledon stage, inducing the embryos as early as the torpedo-shaped stage with a source of abscisic acid at an effective concentration of abscisic acid and for a sufficient period of time to cause expression of desiccation tolerance which includes change in cellular metabolism, electron transport processes and oxidation-reduction reactions in the embryos and drying the induced embryos to provide stable viable embryos.

Abstract: A method is provided for selecting starch-containing vegetables which are subject to low temperature sweetening based on the quality of product to be derived from said vegetables when processed in a manner which can result in undesirable product coloration due to chemical changes of surface sugars on the prepared product, a predetermined minimum level of starch granule crystallinity being established for the vegetable to be processed, above which minimal level an acceptable quality of processed product having an acceptable level of coloration is assured. The method comprises measuring starch granule crystallinity of the vegetable to be processed and selecting for later processing vegetables having a measured starch granule crystallinity above a predetermined minimum level which indicates that the vegetable will resist low temperature sweetening.

Abstract: A serum-free vaccine effective against pneumonic pasteurellosis in cattle comprising a non-toxic leukotoxin specific for ruminant leukocytes is disclosed. The leukotoxin is prepared in a serum-free medium from a culture of Pasteurella haemolytica. The produced leukotoxin is harvested from the culture medium upon detecting a certain stage during the logarithmic phase of the cell growth to obtain the optimum concentration of produced cytotoxin in the serum-free medium. Cattle may be treated with the vaccine to develop anti-leukotoxic immunity to pneumonic pasterellosis.

Abstract: Synthetic novel ellagic acid congeners of the formula: ##STR1## wherein: R.sub.1 is --H, --OH or --OR';R.sub.2 is --H, --OH or --OR';R.sub.3 is --H, --OH or --OR';R.sub.4 is --H, --OH or --OR';R.sub.5 is --H, --OH or --OR';R.sub.6 is --H, --OH or --OR';R' is lower alkyl having 1 to 8 carbon atoms or --CH.sub.2 --Ar;Ar is phenyl or substituted phenyl, and at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5 and R.sub.6 is other than --H. The above compounds and 6H-dibenzo[b,d]pyran-6-one are useful for inhibiting mutagenic activity in living cells.