Abstract: Methods for imaging a sample using fluorescence microscopy, systems for imaging a sample using fluorescence microscopy, and illumination systems for fluorescence microscopes. In some examples, a method includes positioning the sample such that a plane of interest of the sample is coplanar with a focal plane of a detection objective of a microscope. The method includes positioning a paraboloidal mirror around the sample such that a focal point of the paraboloidal mirror is coplanar with the focal plane of the detection objective and the plane of interest of the sample. The method includes directing a beam of annularly collimated excitation light on the paraboloidal mirror to focus a disc of light on the sample and thereby to provide 360 degree lateral illumination of the sample. The method includes imaging the sample through the detection objective.
Type:
Grant
Filed:
March 12, 2019
Date of Patent:
April 26, 2022
Assignee:
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
Inventors:
Paul Samuel Maddox, Tanner Christian Fadero
Abstract: The invention relates to compounds of Formula (I), (Ia), (Ib), (II) or (III), salts thereof, pharmaceutical compositions thereof, as well as therapeutic methods of treatment and prevention.
Type:
Application
Filed:
August 6, 2021
Publication date:
April 21, 2022
Applicants:
GlaxoSmithKline Intellectual Property Development Limited, The University of North Carolina at Chapel Hill, ViiV Healthcare Company
Inventors:
Martha DE LA ROSA, Richard M. DUNHAM, David MARGOLIS, Vincent Wing-Fai TAI, Jun TANG
Abstract: Apparatuses, systems, and methods for preclinical ultrasound imaging of subjects are provided. In one aspect, the apparatus can include a platform on which a subject is positionable and at least one motion stage for controlling a spatial position of at least one ultrasound transducer relative to the platform in order to acquire ultrasound image data of the subject. Methods for preclinical ultrasound raster scanning of at least one organ or tissue in a subject are also provided, where the at least one organ or tissue is a heart.
Type:
Grant
Filed:
January 25, 2016
Date of Patent:
April 19, 2022
Assignees:
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, SONOVOL, INC.
Inventors:
Paul Alexander Dayton, Ryan Christopher Gessner, James Owen Butler, Max Stephan Harlacher, Nicholas Allan Norman
Abstract: The present invention is directed to a hemostatic textile, comprising: a material comprising a combination of glass fibers and one or more secondary fibers selected from the group consisting of silk fibers; ceramic fibers; raw or regenerated bamboo fibers; cotton fibers; rayon fibers; linen fibers; ramie fibers; jute fibers; sisal fibers; flax fibers; soybean fibers; corn fibers; hemp fibers; lyocel fibers; wool; lactide and/or glycolide polymers; lactide/glycolide copolymers; silicate fibers; polyimide fibers; feldspar fibers; zeolite fibers, zeolite-containing fibers, acetate fibers; and combinations thereof; the hemostatic textile capable of activating hemostatic systems in the body when applied to a wound. Additional cofactors such as thrombin and hemostatic agents such as RL platelets, RL blood cells; fibrin, fibrinogen, and combinations thereof may also be incorporated into the textile. The invention is also directed to methods of producing the textile, and methods of using the textile to stop bleeding.
Type:
Grant
Filed:
August 24, 2018
Date of Patent:
April 19, 2022
Assignees:
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, ENIEGRION INC
Inventors:
Thomas H. Fischer, E. Stan Eskridge, William M. Malloy, Malloy Evans
Abstract: Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule.
Type:
Grant
Filed:
January 17, 2020
Date of Patent:
April 19, 2022
Assignee:
The University of North Carolina at Chapel Hill
Abstract: This present disclosure provides devices, systems, and methods relating to the management of a subject's medical needs involving safely accessing a body cavity. In particular, the present disclosure is directed to body cavity conduit devices and systems that facilitate repeated access to a subject's body cavity for removing fluid or performing a procedure in a manner that provides enhanced comfort, safety, and efficacy.
Type:
Application
Filed:
October 6, 2021
Publication date:
April 14, 2022
Applicant:
The University of North Carolina at Chapel Hill
Abstract: In one aspect, composite carbide compositions are described herein which can facilitate the efficient and/or economical manufacture of articles comprising SiC. Briefly, a composite carbide composition comprises silicon carbide (SiC) particles and a silica interparticle phase covalently bonded to the SiC particles.
Type:
Grant
Filed:
January 18, 2018
Date of Patent:
April 5, 2022
Assignee:
THE UNIVERSITY OF NORTH CAROLINA AT CHARLOTTE
Abstract: Silicon carbide (SiC) is an inert material with excellent biocompatibility properties. The biocompatibility is associated with the chemical inertness of the material. Tissue response to inert material is the formation of thin fibrous capsule. In some embodiments described herein, the conversion of SiC from inert material to bioactive material capable of stimulating cell function and making direct bond with tissue is described and the body response to bioactive materials is direct binding without any fibrous capsule.
Type:
Grant
Filed:
December 17, 2020
Date of Patent:
March 29, 2022
Assignee:
THE UNIVERSITY OF NORTH CAROLINA AT CHARLOTTE
Abstract: The invention provides pharmaceutical compositions and methods of use thereof for treating Prader-Willi syndrome. More specifically, the invention provides pharmaceutical compositions that when administered inhibit the G9a driven methylation of histone H3 lysine 9.
Type:
Grant
Filed:
May 17, 2017
Date of Patent:
March 22, 2022
Assignees:
Duke University, The University of North Carolina at Chapel Hill
Inventors:
Yong-hui Jiang, Yuna Kim, Hyeong-min Lee, Jian Jin, Bryan L. Roth
Abstract: A cell processing system, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells are disclosed. Particularly, the cell processing system includes a fluidics cartridge and a control instrument. The fluidics cartridge includes a cell processing chamber that has a micropost array therein, a sample reservoir and a wash reservoir that supply the cell processing chamber, and a waste reservoir and an eluent reservoir at the output of the cell processing chamber. A micropost actuation mechanism and a cell counting mechanism are provided in close proximity to the cell processing chamber. A method is provided of using the cell processing system to collect, wash, and recover cells. Another method is provided of using the cell processing system to collect, wash, count, and recover cells at a predetermined cell density.
Type:
Application
Filed:
November 23, 2021
Publication date:
March 17, 2022
Applicants:
Redbud Labs, Inc., THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
Inventors:
Richard Chasen Spero, Jay Kenneth Fisher, Richard Superfine
Abstract: Methods, systems, and computer readable media for utilizing brain structural characteristics for predicting a diagnosis of a neurobehavioral disorder are disclosed. One method for utilizing brain structural characteristics for predicting a diagnosis of a neurobehavioral disorder includes receiving brain imaging data for a human subject of at least one first age. The method also includes determining, from the brain imaging data, measurements of brain structural characteristics of the human subject and inputting the brain structural characteristics into a model that predicts, using the measurements of the brain structural characteristics, a diagnosis of a neurobehavioral disorder at a second age greater than the at least one first age.
Type:
Grant
Filed:
December 28, 2018
Date of Patent:
March 15, 2022
Assignees:
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, COLLEGE OF CHARLESTON, THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIVERSITY
Inventors:
Martin Andreas Styner, Sun Hyung Kim, Donald Louis Collins, Vladimir S. Fonov, Brent Carl Munsell, Joseph Piven, Heather Cody Hazlett
Abstract: The present invention relates to a method, a system, and a kit of parts for cleaning a surface contaminated with a hazardous drug or a hazardous drug related product, which involves cleaning in succession with a quaternary ammonium solution followed by an isopropyl alcohol solution.
Type:
Grant
Filed:
April 3, 2020
Date of Patent:
March 15, 2022
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
William Zamboni, Tom O'Neill, Stephen Eckel
Abstract: Methods for imaging a sample using fluorescence microscopy, systems for imaging a sample using fluorescence microscopy, and illumination systems for fluorescence microscopes. In some examples, a method includes positioning a dual convex paraboloidal mirror enclosure around the sample. The dual convex paraboloidal mirror enclosure includes an upper paraboloidal mirror and a lower paraboloidal mirror oriented antiparallel to each other. An aperture is defined in the lower paraboloidal mirror, a hemispherical dome is mounted in the aperture, and the sample is surrounded by the hemispherical dome. The method includes directing excitation light onto the sample to form a primary image at an upper vertex of the upper paraboloidal mirror and a secondary image at a lower vertex of the lower paraboloidal minor. The method includes imaging the sample through a detection objective of a microscope.
Type:
Grant
Filed:
March 12, 2019
Date of Patent:
March 1, 2022
Assignee:
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
Inventors:
Paul Samuel Maddox, Tanner Christian Fadero
Abstract: The present disclosure relates to chemical compounds that inhibit HP1-mediated heterochromatin formation, pharmaceutical compositions containing such compounds, methods of identifying such compounds, and their use in the treatment of disorders related to heterochromatin formation such as, for example, a disorder of cellular proliferation (e.g., cancer). This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
Type:
Grant
Filed:
June 29, 2018
Date of Patent:
February 22, 2022
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
Nate Hathaway, Jian Jin, Ian MacDonald, Kyle Butler
Abstract: The present disclosure provides methods and compositions of modified oligonucleotide primer and probe combinations, structurally modified with locked nucleic acids, quenchers, and dyes, effective to detect tumor-derived Human Papilloma Virus (HPV) and tumor-derived Epstein-Barr virus (EBV) and, especially, to distinguish viral DNA derived from tumors from viral DNA derived from infectious viral particles.
Type:
Grant
Filed:
March 16, 2021
Date of Patent:
February 22, 2022
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
Gaorav Gupta, Bhishamjit S. Chera, Sunil Kumar
Abstract: The present invention provides compositions and methods of use comprising a chimeric dengue virus E glycoprotein comprising a dengue virus E glycoprotein backbone, which comprises amino acid substitutions that introduce a dengue virus E glycoprotein epitope from a dengue virus serotype that is different from the dengue virus serotype of the dengue virus E glycoprotein backbone.
Type:
Grant
Filed:
May 23, 2018
Date of Patent:
February 8, 2022
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
Ralph Baric, Matthew Begley, Douglas Widman, Aravinda Desilva, Usha Nivarthi, Boyd Yount, Ellen Young
Abstract: This invention relates to SARS-CoV-2 viruses adapted with nanoluciferase reporter molecules and mouse-adapted SARS-CoV-2 viruses, compositions including the same and methods of use thereof.
Type:
Grant
Filed:
February 11, 2021
Date of Patent:
January 18, 2022
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
Ralph Baric, Kenneth Harold Dinnon, III, Sarah Rebecca Leist
Abstract: Geometrically complex intravaginal rings, systems and methods of making the same are provided herein. Disclosed herein are geometrically complex intravaginal rings with tunable and enhanced drug release, which in some embodiments can be fabricated by 3D printing technologies. The disclosed IVRs include a ring structure comprising a plurality of unit cells or macroscopic and/or microscopic architecture, which can be tuned to control the loading capacity of an active compound within the IVR, the diffusion of an active compound from the IVR, the surface area of the IVR, and/or the mechanical properties of the IVR. The disclosed geometrically complex IVRs can provide superior control over drug loading and drug release compared to conventional IVRs fabricated by injection molding or hot-melt extrusion.
Type:
Grant
Filed:
September 24, 2018
Date of Patent:
January 11, 2022
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
Soumya Rahima Benhabbour, Rima Janusziewicz, Sue J. Mecham
Abstract: The subject matter described herein is directed to methods of modifying the micro-environment of a target cell or The methods comprise systemically administering to a subject a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap in the target cell, wherein the trap is expressed in the target cell thereby modifiying the micro-environment. Also described herein are methods of reducing metastasis of a cancer comprising, systemically administering to a subject suffering from the cancer, a composition comprising a vector, wherein the vector comprises a construct for the expression of a trap, wherein the trap is delivered to and then expressed in tissue susceptible to metastasis, wherein metastasis of the cancer to the tissue is reduced. Compositions for carrying out the methods are also described.
Type:
Grant
Filed:
September 15, 2016
Date of Patent:
January 11, 2022
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
Leaf Huang, Tyler Goodwin, Rihe Liu, Lei Miao
Abstract: Various examples are provided related to diode clamped solid-state circuit breakers (SSCBs). Their configuration allows operation of the SSCB without dynamic voltage balancing issues. In one example, a diode clamped SSCB includes source-side switches and line-side switches connected between a DC source connection and a line-side connection. Clamping capacitors are connected at a common connection point between the source-side and line side switches and source-side and line-side clamping diodes are connected between the source-side switches and line-side switches and the clamping capacitors. Sequential switching of the source-side switches or line-side switches can avoid dynamic voltage balancing issues.
Type:
Grant
Filed:
September 4, 2020
Date of Patent:
January 4, 2022
Assignee:
University of North Carolina Charlotte
Inventors:
Madhav Manjrekar, Ehab H. Shoubaki, Tiancan Pang