Abstract: Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between sub-cellular compartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing ?-galactosidase fragments are provided. These ?-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided.
Type:
Grant
Filed:
September 20, 2011
Date of Patent:
March 25, 2014
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Abstract: A testing method of nucleic acid binding protein based on biochip, comprises the following steps: 1. puts a plurality of groups solution including nucleic acid captured probes into biological sample including a plurality of nucleic acid binding protein to be test, and thus forming nucleic acid captured probe-nucleic acid binding protein complexes; such nucleic acid captured probe includes at least a segment of binding sequence which can bind with aimed nucleic acid binding protein; 2. separates such nucleic acid captured probe-nucleic acid binding protein complexes, then recoveries nucleic acid captured probes; 3. hybridizes the nucleic acid captured probes according to step 2 with a plurality of single strand blotting probes on biochip substrate; the sequence of such blotting probe compensates with such nucleic acid captured probe or one of its strand; 4. detects the result of hybridization.
Type:
Grant
Filed:
November 23, 2004
Date of Patent:
March 25, 2014
Assignees:
CapitalBio Corporation, Tsinghua University
Abstract: The present invention relates to targeting peptides capable of specifically binding to microbial organisms (e.g., P. aeruginosa or S. mutans), antimicrobial peptides having antimicrobial activities, and specifically/selectively targeted antimicrobial peptides (STAMPs). In addition, the present invention provides methods of selectively killing or inhibiting microbial organisms by using the peptides or compositions provided by the present invention.
Type:
Grant
Filed:
November 2, 2010
Date of Patent:
March 25, 2014
Assignees:
The Regents of the University of California, C3 Jian, Inc.
Inventors:
Randal H. Eckert, Daniel K. Yarbrough, Wenyuan Shi, Maxwell H. Anderson, Fengxia Qi, Jian He, Ian H. McHardy
Abstract: The combination of compounds of the hydroxytolan family with ascorbate plus naphthoquinone (Vitamin K3; VK3), or a quinone or semiquinone analogue of VK3, kill tumor cells, inhibit tumor growth and development, and treat cancer in subjects in need thereof.
Type:
Grant
Filed:
June 20, 2008
Date of Patent:
March 25, 2014
Assignees:
Kent State University, Summa Health System
Inventors:
Chun-che Tsai, James M. Jamison, Jackie L. Summers
Abstract: The present invention discloses a phosphor material for organic optoelectronic devices. The phosphor is a complex comprising a metal (Ir or Pt) and an aryl-modified beta-diketone ligand. The complexes of this invention are useful to function as an emitter in the emissive layer of an organic light emitting diode, even as the complex is the main component of this layer.
Abstract: An apparatus for detecting fixation of at least one eye of a subject on a target includes an optical illumination system, an optical detection system, and a signal processing system adapted to communicate with the optical detection system. The signal processing system is configured to calculate at least one of a time-frequency distribution or an Auto-Regressive spectral estimation of the detection signal to provide a frequency distribution of the detection signal at a plurality of times for use in determining periods in which the subject's eye is fixed on the target based on the first and second frequency signatures.
Type:
Grant
Filed:
March 9, 2012
Date of Patent:
March 25, 2014
Assignee:
The Johns Hopkins University
Inventors:
Boris I. Gramatikov, David L. Guyton, Kristina Irsch
Abstract: The present invention provides for increasing fluorescence detection in surface assay systems while increasing kinetics of a bioreaction therein by providing low-power microwaves to irradiate metallic materials within the system in an amount sufficient to increase heat thereby affecting the kinetics of a bioreaction therein.
Type:
Grant
Filed:
November 27, 2012
Date of Patent:
March 25, 2014
Assignee:
University of Maryland, Baltimore County
Abstract: The present invention relates to a biomarker associated with a cancer and a method using the biomarker to evaluate a risk of proliferation, invasion, or metastasis of a cancer. The method of the present invention comprises the following steps: (A) providing a tissue sample to evaluate for risk of proliferation, invasion, or metastasis of a cancer, wherein the tissue sample comprises a non-cancer region, and a suspected cancer region; (B) detecting expression levels of a biomarker and a predetermined standard in the non-cancer region and the suspected cancer region respectively, wherein the biomarker is T-cell lymphoma invasion and metastasis 2 (TIAM2); (C) comparing the expression levels of the biomarker and the predetermined standard in the non-cancer region to the expression levels of the biomarker and the predetermined standard in the suspected cancer region.
Abstract: The present invention discloses compositions and methods for treating diseases such as cancer by targeting luteinizing hormone (LH) or its receptor (LHR) involved in androgen synthesis or testosterone production.
Abstract: Prochelator compounds of Formula (I) or Formula (II) are described, along with methods of using the same and pharmaceutical formulations or compositions containing the same.
Abstract: Enveloped virus vectors are described which comprise a cellular virus receptor protein and which are capable of fusing with a cell which comprises a viral envelope protein to which the cellular virus receptor protein is cognate. Enveloped virus vectors comprising a plurality of cellular virus receptor proteins are also described. Methods for making the enveloped virus vectors are described, as are methods of using the enveloped virus vectors. The invention further relates to a lipoparticle comprising a membrane spanning protein, and the lipoparticle can be attached to a sensor surface. The invention relates to methods of producing and using the lipoparticle to, inter alia, assess protein binding interactions.
Type:
Grant
Filed:
March 15, 2012
Date of Patent:
March 25, 2014
Assignee:
The Trustees of the University of Pennsylvania
Inventors:
Robert Doms, Joseph Rucker, Trevor L Hoffman, Paul Bates, James A Hoxie, Michael J Endres, John Balliet, Dennis L Kolson
Abstract: A power splitter and/or combiner is described. The power splitter may be provided as a broadband, passive, divide by N power splitter that may be advantageously employed in providing power to multiple electrodes within a plasma source. The power splitter comprises a transmission line and a plurality of N secondary windings arranged about the transmission lines.
Abstract: Amorphous mixed metal oxides are used as a feedstock for thermal spray coating. Compared to thermal spray coating methods utilizing crystalline metal oxide feedstocks, the present method reduces energy consumption and expands the range of feedstock chemical compositions. The present method also produces coatings with improved chemical homogeneity. Methods of preparing the amorphous mixed metal oxides are also described.
Type:
Grant
Filed:
January 19, 2011
Date of Patent:
March 25, 2014
Assignee:
The University of Connecticut
Inventors:
Eric Hopkins Jordan, Maurice Gell, Dianying Chen
Abstract: An in-situ gel ophthalmic drug delivery system for estrogen containing an estrogen, a solubilizing and complexing agent, a humectant, a sorbic acid or sorbic acid based stabilizer, an optional chelating agent and gellan gum in the amount of about 0.1-0.5% w/v, optionally 0.1-0.3% w/v, of the composition.
Type:
Grant
Filed:
September 28, 2010
Date of Patent:
March 25, 2014
Assignee:
Duquesne University of the Holy Spririt
Inventors:
Moji Christianah Adeyeye, Vicki L. Davis, Uday K. Kotreka
Abstract: This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.
Type:
Grant
Filed:
November 4, 2009
Date of Patent:
March 25, 2014
Assignees:
The Regents of the University of California, Life Technologies Corporation
Inventors:
Roger Y. Tsien, Roger Heim, Andrew Cubitt
Abstract: A magnetoresistive element according to an embodiment includes a first magnetic layer, a second magnetic layer, and a first nonmagnetic layer provided between the first magnetic layer and the second magnetic layer, the first magnetic layer including a magnetic film of MnxGey (77 atm %?x?82 atm %, 18 atm %?y?23 atm %, x+y=100 atm %).
Type:
Grant
Filed:
March 14, 2013
Date of Patent:
March 25, 2014
Assignees:
Kabushiki Kaisha Toshiba, Tohoku University
Abstract: A method of extracting data from an identifiable monochromatic pattern. The method comprises separating a polychromatic optical signal, received from an object having identifiable monochromatic pattern, into a plurality of wavelength components, separately capturing each of the wavelength components, reconstructing a plurality of images each from a different wavelength component, detecting the identifiable monochromatic pattern in one or more of the images, and extracting data associated with or encoded by the detected identifiable monochromatic pattern. The images have different depths of field.
Type:
Grant
Filed:
March 10, 2011
Date of Patent:
March 25, 2014
Assignee:
Ramot at Tel-Aviv University Ltd.
Inventors:
Benjamin Milgrom, Emanuel Marom, Naim Konforti, Michael Golub
Abstract: A method and system for producing accented image data for an accented image is disclosed. The method includes decomposing each of a first and a second image into a gradient representation which comprises spectral and edge components. The first image comprises more spectral dimensions than the second image. The edge component from the first image is combined with the spectral component from the second image to form a combined gradient representation. Accented image data for the accented image is then generated from data including the combined gradient representation.
Type:
Grant
Filed:
August 27, 2010
Date of Patent:
March 25, 2014
Assignee:
University of East Anglia
Inventors:
David Connah, Mark S. Drew, Graham Finlayson
Abstract: Systems, methods, and computer-readable media for generating a data set are provided. One method includes generating a data set based on input data using a plurality of brokers. The method further includes receiving a request from a user and determining whether the request can be fulfilled using data currently in the data set. When the request can be fulfilled using data currently in the data set, the data is accessed using broker(s) configured to provide access to data within the data set. When the request cannot be fulfilled using data currently in the data set, at least one new broker is spawned using existing broker(s) and additional data needed to fulfill the request is added to the data set using the new broker. The method further includes generating a response to the request using one or more of the plurality of brokers.
Type:
Grant
Filed:
March 12, 2013
Date of Patent:
March 25, 2014
Assignee:
Virginia Polytechnic Institute and State University
Inventors:
Christopher L. Barrett, Madhav V. Marathe, Keith R. Bisset, Paula E. Stretz
Abstract: The invention provides formulations comprising isolated lacto-N-tetraose (LNT) or galactooligosaccharides (GOS) or variants, isomers, analogs and derivatives thereof and a pharmaceutically acceptable carrier.
Type:
Application
Filed:
February 3, 2012
Publication date:
March 20, 2014
Applicant:
The Regents of the University of California
Inventors:
Lars Bode, Tineke Lauwaet, Evelyn Jantscher-Krenn, Frances Gillin