Abstract: A bioreactor device includes a solid substrate having a first face and a second face. The solid substrate at least partially defines a perfusion channel, a plurality of chambers, a fluidic inlet, and a fluidic outlet. A first sheet disposed over the first face and a second sheet disposed over the second face. Characteristically, the combination of the solid substrate, the first sheet and the second sheet define the perfusion channel and each chamber of the plurality of chambers. The plurality of chambers are arranged in rows of chambers in which adjacent chambers are positioned at opposite side of the perfusion channel. The perfusion channel extends from the fluidic inlet and the fluidic outlet having a serpentine path along each row of chambers with each chamber being in fluid communication with the perfusion channel.

Abstract: Disclosed are an expression cassette for isopropanol production, a recombinant vector for isopropanol production expression cassette, a including the recombinant microorganism for isopropanol production into which the vector is introduced, and a method of producing isopropanol using the recombinant microorganism. The recombinant microorganism in which a succinic acid bypass metabolic pathway is introduced to an isopropanol production pathway has very high ability to produce isopropanol. The recombinant microorganism is capable of producing isopropanol in an amount corresponding to about 100 times the maximum amount of isopropanol that is produced using known Corynebacterium glutamicum, and thus can effectively produce isopropanol and can be useful in various industrial fields where isopropanol is utilized.

Abstract: Provided are compositions and methods for selectively reducing the amount of antibiotic resistant and/or virulent bacteria in a mixed bacteria population, or for reducing any other type of unwanted bacteria in a mixed bacteria population. The compositions and methods involve targeting bacteria that are differentiated from other members of the population by at least one unique clustered regularly interspaced short palindromic repeats (CRISPR) targeted DNA sequence. The compositions and methods can be readily adapted to target any bacteria or any bacteria plasmid, or both.

Abstract: A method of collecting resin beads includes first to fourth steps. The first step is a step of preparing a sample on a thin film provided on an upper surface of a substrate. The second step is a step of irradiating the thin film with a laser beam and a laser beam with the laser beam and the laser beam being distant from each other. The third step is a step of producing a microbubble at a position irradiated with the laser beam and producing a microbubble at a position irradiated with the laser beam, by heating the sample by irradiation with the laser beams. The fourth step is a step of collecting a plurality of resin beads in a region between the microbubble and the microbubble by producing convection of the sample in a direction perpendicular to a direction of alignment of the microbubble and the microbubble.

Abstract: A health alert device includes a physiological sensor and processing circuitry. The physiological sensor captures physiological data, including heart rate and blood oxygen data, of a detainee. The processing circuitry repeatedly receives the physiological data, calculates a health metric parameter based on a combination of the physiological data, determines a health metric baseline based on a series of health metric parameters determined over a baseline determination duration, determines first and second risk alert thresholds based on the health metric baseline, determines a health metric delta based on newly received physiological data and the health metric baseline, and transmits first and second risk alert communications to an alert device to cause the alert device to display first and second risk alerts on an alert device display in response to the health metric delta exceeding the first and second risk alert thresholds, respectively.

Abstract: Systems and methods for stimulation of neurological tissue generate stimulation trains with temporal patterns of stimulation, in which the interval between electrical pulses (the inter-pulse intervals) changes or varies over time. Compared to conventional continuous, high rate pulse trains having regular (i.e., constant) inter-pulse intervals, the non-regular (i.e., not constant) pulse patterns or trains that embody features of the invention provide a lower average frequency.

Abstract: Disclosed is a method for preparing a heterogeneous metal composite structure for medical implantation, including the steps of: step 1, preparing titanium alloy powder into a porous skeleton according to different printing strategies; step 2, filling magnesium after being melted into pores of the porous skeleton; and step 3, cooling a titanium-magnesium interpenetrating phase composite structure prepared in step 2 to room temperature, and covering a surface of the titanium-magnesium interpenetrating phase composite structure with a hydroxyapatite coating. In the present disclosure, a porous lattice dot-array structure of titanium alloy is used as a skeleton, and the skeleton pore is filled by pressureless infiltration of magnesium or hot isostatic pressure.

Abstract: The present disclosure provides methods for determining the presence or absence of and/or the etiology of an acute respiratory viral infection in a subject, as well as methods of treating the subject based on the determination, by measuring on a platform the expression levels of a pre-defined set of gene products.

Abstract: Assays for compounds having a desired biological activity against one but not both of a pair of microorganism species utilize separately detectable labels. The first and second microorganisms are different from each other, the first microorganisms exhibit a first detectable label, the second microorganisms exhibit a second detectable label different from the first detectable label, and neither of the labels interferes with detection of the other label. In various embodiments, the first and second microorganisms are incubated with a candidate compound or a producer thereof. The different labels permit isolation of microorganisms exhibiting one of the labels but not the other, indicating the desired activity, and the compound responsible for this differential response is isolated.

Abstract: Provided herein are compositions and methods for treating or preventing a neurodegenerative disease, a myodegenerative disease, a prion disease or a lysosomal storage disease in a subject.

Abstract: An attraction system of an amusement park includes a viewing area, a virtual area, and a beam splitter. The beam splitter is positioned to enable visibility of a portion of the viewing area through the beam splitter and visibility of a portion of the virtual area via reflection off the beam splitter. The attraction system also includes a controller configured to perform operations. The operations include generating image data that is distorted in correlation to a distortive parameter associated with a first element disposed in the viewing area. The distortive parameter is indicative of a distortion applied by the first element to light interacting with the first element. The operations also include transmitting the image data to cause display of a second element in the virtual area.

Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.

Abstract: A method for treating or inhibiting the progression of placental insufficiency syndrome in a subject in need thereof is provided. The method includes administering to the subject a therapeutically effective amount of a composition including irisin.

Abstract: The present invention provides a longitudinally split immature tiller separated from a rhizome (LSITR), a cluster of multiple in vitro shoots (CMIT), a cluster of stem segments containing shoot primordia (CSSSP) and an in vitro tiller of Miscanthus x giganteus (Giant miscanthus), and their uses in propagating Miscanthus x giganteus (Giant miscanthus).

Abstract: The present invention provides a cable network planning method comprising: modelling the prespecified topology as a Steiner tree including a plurality of terminal nodes representing a plurality of cable landing stations (CLSs) to be installed respectively in a plurality of destination regions connected by the cable network, a plurality of Steiner nodes presenting a plurality of branching units (BUs) to be installed in the cable network, and a plurality of edges connecting the terminal nodes and Steiner nodes; constructing a directed acyclic graph (DAG); finding a Steiner minimal tree (SMT) by applying a DAG-Least-Cost-System algorithm; returning coordinates of Steiner nodes, terminal nodes of the SMT as locations of the BUs and the CLSs. The provided method enables inclusion of costs of the Steiner nodes by taking account of Steiner nodes with degree in excess of three such that more flexible BU branching structures and CLS locations alternatives can be considered.

Abstract: The disclosure provides polypeptides and pharmaceutical compositions thereof. Such polypeptides can be useful, for example, in treating pain in a subject. In some embodiments, the polypeptides are useful for treating neuropathic and/or inflammatory pain.

Abstract: Systems and methods of using and imaging shear-thinning biomaterial compositions are provided. More particularly, systems and methods of providing reversible birth control and/or for delivering therapeutics to reproductive organs are provided. A method includes administering a biomaterial into fallopian tubes, uterus, or vas deferens of the subject, where the biomaterial is a shear-thinning nanocomposite.

Abstract: The present disclosure describes, in part, an enzyme-mediated radical initiating system and methods of using the system to produce polymers, including polymeric hydrogels, at ambient conditions.

Abstract: The present invention relates to the technical field of wastewater treatment, and discloses a bioaugmentation treatment process for lithium battery producing wastewater. The method comprises the following steps: 1) introducing wastewater into a hydrolytic acidification tank, and adding Enterobacter sp. NJUST50 and activated sludge to the hydrolytic acidification tank for hydrolytic acidification treatment; 2) introducing the effluent into an anoxic tank, and adding Enterobacter sp. NJUST50 and anaerobic activated sludge for anoxic treatment; 3) introducing the effluent into an aerobic tank, and adding Enterobacter sp. NJUST50 and aerobic activated sludge for aerobic treatment; 4) introducing the effluent into an anoxic filter tank, and adding Enterobacter sp. NJUST50 and anaerobic activated sludge to the filter tank for treatment; and 5) introducing the effluent into a biological aerated filter tank, and adding a sludge mixture of Enterobacter sp.

Abstract: An electrode structure for a secondary battery includes a potential sheath capable of suppressing a side reaction between an electrode and an electrolyte through electric potential control. The electrode structure for the secondary battery uses the electric potential control so that an unstable SEI layer, which causes decrease in cycle characteristic and capacity of an anode material, occurs only on the surface of a potential sheath without occurring on the surface of the anode active material, thereby being capable of completely solving the problems of the existing nanostructured electrode.