Abstract: A chromatographic column assembly is described, comprising a channel and a flow inducer. The channel comprises an inlet for receiving a sample liquid and an outlet, and is adapted for separating the sample liquid into components when the sample liquid flows through the channel in an axial direction from the inlet to the outlet. The flow inducer is adapted for controllably inducing, when the sample fluid is flowing through the channel, a motion of the sample liquid in the channel in a plane substantially orthogonal to the axial direction.

Abstract: The present invention relates to nucleic acid regulatory elements that are able to enhance muscle-specific expression of genes, in particular expression in cardiac muscle and/or skeletal muscle, methods employing these regulatory elements and uses of these elements. Expression cassettes and vectors containing these nucleic acid regulatory elements are also disclosed. The present invention is particularly useful for applications using gene therapy, more particularly muscle-directed gene therapy, and for vaccination purposes.

Abstract: The disclosed technology generally relates to semiconductor fabrication and more particularly to forming vertical transistor devices. In an aspect, a method of forming a vertical transistor device includes forming, on a substrate, a fin comprising a stack including a first layer, a second layer formed above the first layer and a third layer formed above the second layer. The method additionally includes forming a gate layer serving as an etch mask above the third layer. The method further includes etching the second and third layers of the fin using the gate layer as the etch mask to form a pillar. First and third layers of the pillar define a source region and a drain region, respectively, of the vertical transistor device. A second layer of the pillar defines a channel region of the vertical transistor device. The gate layer comprises a gate electrode arranged on at least one sidewall of the second layer.

Abstract: A prosthesis or orthosis having a movement controlling mechanism (MCM) including a first MCM part, a second MCM part and one or more intermediate elements and biasing mechanism which, in a contacting mode of operation of the MCM, bias the intermediate elements against a MCM part. When a relative torque or force is applied in a blocking sense (U) transmission of torque is allowed and, on the other hand, when a torque or force is applied in the opposite sense (V) non-blocking relative movement is allowed.

Abstract: A system is described for performing structural health monitoring of an object under study. The system comprises a hollow cavity structure comprising one or more cavities obtained using additive manufacturing. The cavity structure is sealable from its environment and forms an integral part of the object under study. The cavity structure furthermore is connectable to a pressure sensor for sensing a pressure in the cavity structure.

Abstract: The application relates to the field of structural biology. More specifically, the disclosure relates to methods for the identification and characterization of biomolecular tools allowing the selective recognition and/or stabilization of distinct conformational states of protein complexes, including transient protein-protein interactions and protein-nucleic acid complexes. Such tools can then be used for purification purposes, crystallization and structure determination of these stabilized protein complexes, for drug discovery, as research tools, as well as for diagnosis and treatment of diseases.

Abstract: The present disclosure relates to the field of G protein coupled receptor (GPCR) structural biology and signaling. In particular, the present disclosure relates to binding domains directed against and/or specifically binding to GPCR:G protein complexes. Also provided are nucleic acid sequences encoding such binding domains and cells expressing or capable of expressing such binding domains. The binding domains of the present disclosure can be used as universal tools for the structural and functional characterization of G-protein coupled receptors in complex with downstream heterotrimeric G proteins and bound to various natural or synthetic ligands, for investigating the dynamic features of G protein activation, as well as for screening and drug discovery efforts that make use of GPCR:G protein complexes.

Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.

Abstract: Provided are hydrogel-forming peptides that comprise at least one dyad of hydrophobic amino acid residues in which one is a ?3- or ?2-homo amino acid residue. The hydrogels derived from these peptides can be loaded with biological materials and can be used in physiological conditions.

Abstract: An aspect of the disclosure includes a comparator circuit comprising: a master latch comprising a first amplifier circuit and a first latch circuit coupled to an output of the first amplifier circuit; a slave latch comprising a second amplifier circuit having an input coupled to the output of the first amplifier circuit, and a second latch circuit coupled to an output of the second amplifier circuit; and a hysteresis compensation circuit coupled to the output of the second amplifier circuit and configured to cause a first predetermined signal level shift of an output signal of the first amplifier circuit in response to a high signal level at the output of the second amplifier circuit, and configured to cause a second predetermined signal level shift of an output signal of the first amplifier circuit in response to a low signal level at the output of the second amplifier circuit.

Abstract: The present disclosure relates to a front-end module for a telecommunication device with an EBD circuit comprising a hybrid transformer for coupling via a transmit port to the telecommunication device transmitting a first frequency transmit signal, to an antenna, via a receive port to the telecommunication device receiving a second frequency receive signal, and to a tunable impedance circuit. In a first configuration the EBD circuit is configured to isolate the transmit port from the receive port at the first frequency, and the FEM comprises a first filter at the transmit port for attenuating the transmit signal with a predetermined amount at the second frequency. In a second configuration the EBD circuit is configured to isolate the transmit port from the receive port at the second frequency, and the FEM comprises a second filter at the receive port for attenuating the receive signal a predetermined amount at the first frequency.

Abstract: A polar transmitter provided for transmitting a phase/frequency modulated and amplitude modulated transmit signal and a method for generating a transmit signal using a polar transmitter are described. An example polar transmitter comprises a phase locked loop for generating a phase/frequency modulated precursor of the transmit signal. The phase locked loop comprises at its input a phase error detection unit for detecting a phase error of the precursor fed back from the output of the phase locked loop to the phase error detection unit as a feedback signal. The polar transmitter comprises a digital amplitude modulator for amplitude modulation of the precursor, resulting in the transmit signal. The digital amplitude modulator is arranged within the phase locked loop for amplitude modulation of the precursor before being output by the PLL. The phase error detection unit is further provided for detecting the amplitude of the feedback signal.

Abstract: The present invention relates to immunoglobulin single variable domains that bind MIF and more in particular to polypeptides that comprise or essentially consist of one or more such immunoglobulin single variable domains; to nucleic acids encoding such polypeptides; to methods for preparing such polypeptides; to compositions and in particular to pharmaceutical compositions that comprise such polypeptides, for prophylactic, therapeutic or diagnostic purposes.

Abstract: The present invention relates to the field of GPCR structure biology and signaling. In particular, the present invention relates to protein binding domains directed against or capable of specifically binding to a functional conformational state of a G-protein-coupled receptor (GPCR). More specifically, the present invention provides protein binding domains that are capable of increasing the stability of a functional conformational state of a GPCR, in particular, increasing the stability of a GPCR in its active conformational state. The protein binding domains of the present invention can be used as a tool for the structural and functional characterization of G-protein-coupled receptors bound to various natural and synthetic ligands, as well as for screening and drug discovery efforts targeting GPCRs. Moreover, the invention also encompasses the diagnostic, prognostic and therapeutic usefulness of these protein binding domains for GPCR-related diseases.

Abstract: A modular management system for balancing, testing and protecting rechargeable energy storage cells connected in series. Different energy storage cell technologies can be connected in the same battery pack and they can be completely balanced by using one or both of two balancing modes. In addition, the modular management system includes bidirectional and unidirectional switches optionally connected to a single ohmic device such as a resistor to efficiently execute a Dual Function Process (DFP) (i.e., passive/active balancing, and testing mode for SoH/SoC estimation) preferably without using any extra or external components (i.e., capacitor or inductor or DC/DC converter or power supply). The systems and methods decrease the balancing time, energy loss, heat loss and complexity needed to monitor, protect and balance energy storage cell systems such as battery systems, and thus decreasing the overall cost.

Abstract: The present invention relates to vectors containing liver-specific regulatory sequences and codon-optimized factor IX or factor VIII genes, methods employing these vectors and uses of these vectors. Expression cassettes and vectors containing these liver-specific regulatory elements and codon-optimized factor IX or factor VIII genes are also disclosed. The present invention is particularly useful for applications using gene therapy, in particular for the treatment of hemophilia A and B.

Abstract: The invention relates to mutant forms of the outer membrane-located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.

Abstract: Described herein are methods and genetically engineered cells useful for uncapping a mannose-6-phosphate residue on an oligosaccharide.

Abstract: The present invention relates to a composition and method for assisted reproductive technology in mammals. In particular, the present invention provides compositions and methods for in vivo maturation of an immature cumulus oocyte complex (COC), thereby enhancing the embryology outcome.

Abstract: Described are single domain antibodies with a specificity for BACE1. More specifically, described are single variable-domain antibodies derived from camelids that bind to BACE1 and are capable of inhibiting the activity of BACE1. The antibodies can be used for research and medical applications. Specific applications include the use of BACE1-specific antibodies for the treatment of Alzheimer's disease.