Abstract: The present technology provides a description of a flow column dampener device capable of decreasing the MALS noise from solvent contaminants and transient ripples induced by the HPLC pump prior to the evaluation and characterization of the biophysical properties of macromolecules. This dampener typically includes a particle bed column that can trap and remove the noise-inducing soluble particulates from mobile phase, while simultaneously dampening the noise induced by pump-induced ripples. The types of sorbents, sorbent particle size range and column size formats of the device provides significant reduction in MALS noise prior to the evaluation. This device enables confident characterization of cell and gene therapy products with accurate measurements of the physical properties of the macromolecule analytes such as cell and gene therapy products.

Abstract: In various aspects, the present disclosure pertains to sorbents for isolating at least one target protein from a liquid sample, the sorbents comprising a solid support comprising attached at least one attached high affinity reagent with an affinity for the at least one target protein. Other aspects of the present disclosure include kit that contain such sorbents and methods of treating samples using the same.

Abstract: The exemplary embodiments may provide a collective insulating sleeve for a plurality of chromatography columns or may provide separate insulating sleeve for each of the chromatography columns in a plurality. As a result, column ovens are not needed, and pre-heaters may not be required for each chromatography column in some exemplary embodiments. Thus, parallel column arrangements in the exemplary embodiments may be more compact than conventional arrangements.

Abstract: Liquid chromatography systems described herein are smaller in size and with a reduced extra-column volume due to direct connection of a liquid chromatography column and/or detector to an injector valve and due to eliminating the need for a column oven by relying on heating of the liquid chromatography column and detector by a preheater. The size of the liquid chromatography systems are enough that the liquid chromatography systems may be deployed adjacent to automated sample preparation robotics, adjacent to a process stream, or adjacent to the inlet of a mass spectrometer. In addition, the extra-column volume of the liquid chromatography systems may be reduced by eliminating many of the connection tubes and by situating components of the liquid chromatography system in closer proximity.

Abstract: The present technology is directed to a method and kit for extracting oligonucleotides using solid phase extraction. Oligonucleotides are extracted from a biological sample by using a protease-assisted sample pretreatment step and a subsequent weak anion exchange solid phase extraction process.

Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent.

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The chromatographic materials of the invention have controlled porosity and comprise a chromatographic core material and one or more layers of chromatographic surface materials which each independently provide an average pore diameter, an average pore volume, or a specific surface area such that the combined layers form a chromatographic material having a predetermined or desired pattern of porosity from the core material to the outermost surface. The materials are useful for HPLC separations, normal-phase separations, reversed-phase separations, chiral separations, HILIC separations, SFC separations, affinity separations, perfusive separations, partially perfusive separations, and SEC separations.

Abstract: The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

Abstract: A solvent mixing system includes a mixing tee, a centrifugal mixing path, and a low frequency blending mixer. The mixing tee has at least two solvent input ports and a solvent output port in fluid communication with one another. The centrifugal mixing path has a mixing path inlet in fluid communication with the solvent output port of the mixing tee. The centrifugal mixing path includes at least one coiled segment between the mixing path inlet and a mixing path outlet. The low frequency blending mixer is in fluid communication with the outlet of the centrifugal mixing path.

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with an immunoglobulin-binding protein. The functionalized particles of the present disclosure can be used to purify a range of immunoglobulins with affinity to the immunoglobulin-binding protein.

Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with streptavidin. The streptavidin functionalized particles of the present disclosure can be bound to biotinylated antibodies, biotinylated antigen-binding fragments thereof, or biotinylated oligonucleotides to create customizable affinity chromatography columns. That is, the functionalized nonporous polymer particles can be packed in a stainless steel, titanium, or other metal or metal alloy column for affinity high pressure liquid chromatography (HPLC).

Abstract: The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).

Abstract: The present disclosure relates to a filter. The filter includes a porous element, a compression element and a housing. At least a portion of the porous element is coated with an alkylsilyl coating. The compression element is configured to receive the porous element thereby forming an assembly. The housing has an opening formed therein. The opening is configured to receive the assembly. The assembly is retained within the opening when the assembly is received therein.

Abstract: Described herein are systems and methods used for washing needle assemblies with multiple needles.

Abstract: Embodiments described herein relate to improved devices and techniques for measuring the charge carried by analyte particles. The charge may be measured in a way that does not destroy the analyte, so that the analyte remains available for further analysis. The charged analyte particles may pass through (or by) an electrode. In doing so, they induce a counter charge on that electrode which can be detected electronically. Subsequent to their passage through that electrode, the particles can be collected on a substrate or may pass in real-time into a mass spectrometer. In some embodiments, both conventional destructive detection and nondestructive detection may both be present in an improved charged aerosol detector and the stream of charged particles may be directed to one or the other by a suitable redirector. Embodiments may be combined with light scattering analysis to provide further non-destructive analysis. Various combinations of these improvements are also described.

Abstract: The present disclosure relates to a method of separating a sample including a mycotoxin. The method includes flowing the sample through a column to load the sample on the column, washing the column to reduce impurities, eluting the mycotoxin from the column, collecting an eluted sample with the mycotoxin in a sample plate, sealing the eluted sample in the sample plate, transferring the sealed sample plate, removing the eluted sample from the transferred sample plate; and analyzing the removed eluted sample. The column can include an affinity resin, such as, for example, an immunoaffinity resin. The sample plate can be a 96-well plate.

Abstract: The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

Abstract: A sample preparation device is provided for enriching a component of a sample. The sample preparation device includes a surface in fluid communication with the sample, an alkylsilyl coating disposed on the surface, and an affinity ligand or an enzyme covalently bonded to the alkylsilyl coating.

Abstract: The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.

Abstract: In various aspects, the present disclosure pertains to sorbents for isolating at least one target protein from a liquid sample, the sorbents comprising a solid support comprising attached at least one attached high affinity reagent with an affinity for the at least one target protein. Other aspects of the present disclosure include kit that contain such sorbents and methods of treating samples using the same.