Patents Assigned to Waters Technologies Corporation
  • Publication number: 20230295636
    Abstract: A method of capturing human immunoglobulin Fc domains in a biofluid sample is provided. The method includes providing an affinity capture device. The affinity capture device includes a surface having an aptamer that is at least 80% identical to SEQ ID NO 1 immobilized onto the surface of the affinity capture device. The biofluid sample is diluted with a binding buffer. The binding buffer includes (A) tris(hydroxymethyl)aminomethane (Tris), trimethylamine (TES), 2-ethanesulfonic acid (MES), or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); (B) a magnesium cation at a concentration between about 10 µM to about 20 mM; and (C) a total monovalent cation concentration from 0 to no greater than 100 mM. The human immunoglobulin Fc domains in the biofluid sample are adsorbed to the aptamer with the binding buffer.
    Type: Application
    Filed: January 6, 2023
    Publication date: September 21, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Xiaoxiao Liu, Matthew A. Lauber
  • Publication number: 20230294073
    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution.
    Type: Application
    Filed: May 3, 2023
    Publication date: September 21, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Nicole L. Lawrence, Daniel P. Walsh, Jacob Nathan Fairchild, Kevin Daniel Wyndham
  • Publication number: 20230266284
    Abstract: The present disclosure is directed to methods for performing size exclusion chromatographic (SEC) separations. Embodiments of the present disclosure feature methods for improved separations of biomolecule analytes, such as CRISPR-related proteins, nucleotides, and ribonucleoprotein complexes, in SEC, for example, by using hydroxy-terminated polyethylene glycol surface modified stationary phase materials and/or C2/PEG surface modified column hardware.
    Type: Application
    Filed: February 14, 2023
    Publication date: August 24, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Lavelay Kizekai, Stephen Shiner, Matthew Lauber
  • Publication number: 20230256413
    Abstract: The present invention provides the use of charged surface reversed phase chromatographic materials along with standard reversed-phase LC and mass spectrometry compatible conditions for the retention, separation, purification, and characterization of acidic, polar molecules, including, but not limited to, organic acids, ?-amino acids, phosphate sugars, nucleotides, other acidic, polar biologically relevant molecules. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.
    Type: Application
    Filed: April 25, 2023
    Publication date: August 17, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Paul Rainville, Jacob N. Fairchild, Babjide Okandeji, Nicole L. Lawrence, Dimple Shah
  • Publication number: 20230258649
    Abstract: The present invention relates to a method for analyzing a sample, the method comprising: (a) incubating a sample comprising an analyte with at least one enzyme to produce a digestion mixture comprising fragments of the analyte; (b) loading the digestion mixture onto a reversed-phase chromatography column; and (c) performing reversed-phase chromatography on the digestion mixture, wherein the at least one enzyme is hydrophobically modified to increase a retention time of the at least one enzyme such that the at least one enzyme elutes from the reversed-phase chromatography column later than the fragments of the analyte.
    Type: Application
    Filed: February 10, 2023
    Publication date: August 17, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Matthew Lauber, Wenjing Li
  • Publication number: 20230251230
    Abstract: A liquid chromatography apparatus includes a diffusion-bonded separation column comprising a lower substrate comprising titanium, an upper substrate comprising titanium, and a titanium patterned foil disposed between the lower substrate and the upper substrate. The lower substrate, titanium patterned foil, and upper substrate are diffusion bonded together to form a fluid path extending from an inlet port to an outlet port, wherein walls defining the fluid path within the diffusion-bonded separation column include a titanium surface coating.
    Type: Application
    Filed: April 21, 2023
    Publication date: August 10, 2023
    Applicant: Waters Technologies Corporation
    Inventor: Theodore A. Dourdeville
  • Publication number: 20230204478
    Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.
    Type: Application
    Filed: February 9, 2023
    Publication date: June 29, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Bonnie A. Alden, Kenneth Berthelette, Jon Finch, Donna Osterman, Thomas Swann, Thomas H. Walter
  • Publication number: 20230201744
    Abstract: The present disclosure pertains to sample preparation devices useful for affinity capture and purification that include one or more internal structures that comprise a reservoir, a well, a fluid passageway, sorbent particles, and a filter element that blocks passage of the affinity sorbent particles, which sample preparation devices combine the attributes of both dispersive and flow through designs into a single sample preparation device. The present disclosure also pertains to kits that contain and methods that use such sample preparation devices.
    Type: Application
    Filed: March 7, 2023
    Publication date: June 29, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Raymond P. Fisk, Timothy J. Becker, Mary Elizabeth Lame, Frank John Marszaikowski, Matthew A. Lauber
  • Publication number: 20230194543
    Abstract: Provided herein are methods, techniques and processes for selecting one or more peptides for virus detection (e.g., influenza, SARS-CoV-2) in clinical samples using mass spectrometry. Methods for selecting a combination of peptides to identify one or more disease states in a patient can include: (i) selecting the one or more disease states; (ii) collecting information on proteins associated with the one or more disease states being present within the subject; (ii) in silico digesting individual proteins associated with the one or more disease state to obtain possible workflow peptides; (iv) collecting filtering data associated with the possible workflow peptides; (iv) analyzing the possible workflow peptides for coverage of the one or more disease states and for mass spectrometry detection and resolution; and (iv) selecting the combination of peptides from the possible workflow peptides based on the analyzing step.
    Type: Application
    Filed: November 7, 2022
    Publication date: June 22, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Johannes Vissers, Thomas McDonald, Keith Richardson
  • Patent number: 11679341
    Abstract: The present disclosure provides methods for the separation and quantitation of enantiomers of vitamin E using supercritical fluid chromatography (SFC) or carbon dioxide-based chromatography on chiral columns. The disclosed methods may be used to quantitatively determine the concentration of RRR-?-tocopherol in foods, food ingredients, dietary supplements, vitamin premixes, nutritional formulas, and medicines. Further provided is a method of differentiating the source of ?-tocopherol as natural or synthetic.
    Type: Grant
    Filed: April 29, 2021
    Date of Patent: June 20, 2023
    Assignee: Waters Technologies Corporation
    Inventors: Jinchuan Yang, Paul Rainville
  • Publication number: 20230187193
    Abstract: An image charge detection assembly for a mass spectrometer comprising an image charge detector that is housed within an electrically conductive shielding enclosure, and a preamplifier, wherein at least an input stage of the preamplifier, which is electrically connected to the detector, is housed with the detector within the enclosure.
    Type: Application
    Filed: December 14, 2022
    Publication date: June 15, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Joseph A. Jarrell, Patrick Brophy, Alistair J. Schofield
  • Publication number: 20230182114
    Abstract: The present disclosure is directed to surface modified materials such as stationary phase materials for performing size exclusion chromatography. Aspects of the present disclosure feature materials surface modified with a moiety including a polyethylene glycol (PEG) functionality and a moiety comprising a diol functionality. Such surface modified materials exhibit a reduced propensity for ionic and hydrophobic secondary interactions.
    Type: Application
    Filed: November 17, 2022
    Publication date: June 15, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Yeliz Tunc Sarisozen, Nicole L. Lawrence, Darryl W. Brousmiche, MingCheng Xu, Kevin Wyndham
  • Publication number: 20230187198
    Abstract: There is provided a method of operating a charge detection mass spectrometer (CDMS), the CDMS comprising an electrostatic ion trap, the electrostatic ion trap comprising a plurality of electrodes, the method comprising: a) introducing a first ion into the electrostatic ion trap at a first ion energy, b) setting the voltage of the plurality of electrodes to a first voltage map, c) obtaining first CDMS data indicative of a first ion oscillation frequency, d) obtaining an acceptable range or ranges of ion oscillation frequencies, e) changing the first ion energy to a second ion energy and/or changing the first voltage map to a second voltage map, and f) obtaining second CDMS data indicative of a second ion oscillation frequency.
    Type: Application
    Filed: December 14, 2022
    Publication date: June 15, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Joseph A. Jarrell, Patrick Brophy, Kevin Giles
  • Publication number: 20230173406
    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are superficially porous chromatographic particulate materials comprising sized less than 2 microns.
    Type: Application
    Filed: January 13, 2023
    Publication date: June 8, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Kevin D. Wyndham, Jacob N. Fairchild, Pamela C. Iraneta, Stephen J. Shiner, Darryl W. Brousmiche, Daniel P. Walsh
  • Patent number: 11656237
    Abstract: Described is a chain including a plurality of links pivotally connected to each other such that the chain is configured to bend in one direction without back-bending. Each of the plurality of links including a link body include: an inner side facing a bending direction of the chain; an outer side facing opposite the bending direction of the chain; a back-bending prevention portion; a post feature extending laterally across the link body, and a connection feature configured to engage with the post feature of the first other of the pivotally connected plurality of links to create one direction pivotal attachment without back-bending between the links. Further disclosed is a chain system and methods of moving devices with a chain or chain system.
    Type: Grant
    Filed: July 28, 2020
    Date of Patent: May 23, 2023
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventor: Jeffrey Musacchio
  • Patent number: 11656208
    Abstract: A chromatography system includes a gradient delay volume defined as an overall fluid volume between where gradient is proportioned until an inlet of a chromatography column, a pump pumping a flow of gradient; and at least one valve located downstream from the pump, the at least one valve having a plurality of ports including an inlet port that receives the flow of gradient from the pump and an outlet port through which the flow of gradient exits the at least one valve, the at least one valve having at least two positions. A first position of the at least two positions of the at least one valve increases the gradient delay volume of the chromatography system relative to when the at least one valve is in a second position.
    Type: Grant
    Filed: March 19, 2021
    Date of Patent: May 23, 2023
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Sylvain Cormier, Richard W. Andrews, Peyton C. Beals
  • Patent number: 11650141
    Abstract: An actuator control system, mechanical testing system, and method for adaptive control of an actuator of a mechanical testing device is provided. The method includes applying a mechanical load to the specimen with the actuator, resulting in receiving a load sensor signal from a load sensor and a displacement sensor signal from a displacement sensor, deriving lumped dynamics of the mechanical testing device by analyzing a phase and magnitude relationship between a current command of a motor of the mechanical testing device and a resultant deflection of the specimen, and controlling the actuator based on the derived lumped dynamics of the mechanical testing device such that the controlling results in a stable motion.
    Type: Grant
    Filed: August 25, 2021
    Date of Patent: May 16, 2023
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventor: Kevin Alstrin
  • Patent number: 11642653
    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: May 9, 2023
    Assignee: Waters Technologies Corporation
    Inventors: Nicole L. Lawrence, Kevin Daniel Wyndham, Beatrice W. Muriithi
  • Publication number: 20230136953
    Abstract: The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and/or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.
    Type: Application
    Filed: October 28, 2022
    Publication date: May 4, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Jennifer M. Nguyen, Xiaoxiao Liu, Michael Donegan
  • Publication number: 20230125093
    Abstract: The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30° C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.
    Type: Application
    Filed: October 21, 2022
    Publication date: April 27, 2023
    Applicant: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Kevin Wyndham, Szabolcs Fekete, Hua Yang, Stephan Koza