Patents Assigned to Waters Technologies Corporation
  • Publication number: 20240416259
    Abstract: The present technology provides a description of a flow column dampener device capable of decreasing the MALS noise from solvent contaminants and transient ripples induced by the HPLC pump prior to the evaluation and characterization of the biophysical properties of macromolecules. This dampener typically includes a particle bed column that can trap and remove the noise-inducing soluble particulates from mobile phase, while simultaneously dampening the noise induced by pump-induced ripples. The types of sorbents, sorbent particle size range and column size formats of the device provides significant reduction in MALS noise prior to the evaluation. This device enables confident characterization of cell and gene therapy products with accurate measurements of the physical properties of the macromolecule analytes such as cell and gene therapy products.
    Type: Application
    Filed: June 13, 2024
    Publication date: December 19, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Lavelay Kizekai, Michael Dion, Stephen Shiner, Balasubrahmanyam Addepalli, Matthew A. Lauber
  • Publication number: 20240409579
    Abstract: In various aspects, the present disclosure pertains to sorbents for isolating at least one target protein from a liquid sample, the sorbents comprising a solid support comprising attached at least one attached high affinity reagent with an affinity for the at least one target protein. Other aspects of the present disclosure include kit that contain such sorbents and methods of treating samples using the same.
    Type: Application
    Filed: August 19, 2024
    Publication date: December 12, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Laks Iyer, Beatrice W. Muriithi
  • Patent number: 12163934
    Abstract: The exemplary embodiments may provide a collective insulating sleeve for a plurality of chromatography columns or may provide separate insulating sleeve for each of the chromatography columns in a plurality. As a result, column ovens are not needed, and pre-heaters may not be required for each chromatography column in some exemplary embodiments. Thus, parallel column arrangements in the exemplary embodiments may be more compact than conventional arrangements.
    Type: Grant
    Filed: June 22, 2021
    Date of Patent: December 10, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Michael O. Fogwill, Martin Gilar, Joseph D. Michienzi, Wade P. Leveille, Geoff C. Gerhardt, Jeffrey Musacchio, Jason F. Hill, Sylvain G. Cormier
  • Patent number: 12163933
    Abstract: Liquid chromatography systems described herein are smaller in size and with a reduced extra-column volume due to direct connection of a liquid chromatography column and/or detector to an injector valve and due to eliminating the need for a column oven by relying on heating of the liquid chromatography column and detector by a preheater. The size of the liquid chromatography systems are enough that the liquid chromatography systems may be deployed adjacent to automated sample preparation robotics, adjacent to a process stream, or adjacent to the inlet of a mass spectrometer. In addition, the extra-column volume of the liquid chromatography systems may be reduced by eliminating many of the connection tubes and by situating components of the liquid chromatography system in closer proximity.
    Type: Grant
    Filed: May 4, 2022
    Date of Patent: December 10, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Michael O. Fogwill, Fabrice Gritti
  • Publication number: 20240401023
    Abstract: The present technology is directed to a method and kit for extracting oligonucleotides using solid phase extraction. Oligonucleotides are extracted from a biological sample by using a protease-assisted sample pretreatment step and a subsequent weak anion exchange solid phase extraction process.
    Type: Application
    Filed: May 31, 2024
    Publication date: December 5, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Mary Elizabeth Trudeau, Matthew A. Lauber, Nicole Lawrence, Balasubrahmanyam Addepalli
  • Patent number: 12158472
    Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent.
    Type: Grant
    Filed: May 11, 2022
    Date of Patent: December 3, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Matthew A Lauber, Darryl W. Brousmiche, Stephan M. Koza
  • Publication number: 20240390877
    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The chromatographic materials of the invention have controlled porosity and comprise a chromatographic core material and one or more layers of chromatographic surface materials which each independently provide an average pore diameter, an average pore volume, or a specific surface area such that the combined layers form a chromatographic material having a predetermined or desired pattern of porosity from the core material to the outermost surface. The materials are useful for HPLC separations, normal-phase separations, reversed-phase separations, chiral separations, HILIC separations, SFC separations, affinity separations, perfusive separations, partially perfusive separations, and SEC separations.
    Type: Application
    Filed: August 7, 2024
    Publication date: November 28, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Daniel P. Walsh, Kevin Daniel Wyndham, Jacob Nathan Cairchild, Nicole L. Lawrence
  • Patent number: 12138617
    Abstract: The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.
    Type: Grant
    Filed: September 16, 2021
    Date of Patent: November 12, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Nicole L. Lawrence, Yeliz Tunc Sarisozen, Darryl W. Brousmiche, Matthew A. Lauber
  • Publication number: 20240367123
    Abstract: A solvent mixing system includes a mixing tee, a centrifugal mixing path, and a low frequency blending mixer. The mixing tee has at least two solvent input ports and a solvent output port in fluid communication with one another. The centrifugal mixing path has a mixing path inlet in fluid communication with the solvent output port of the mixing tee. The centrifugal mixing path includes at least one coiled segment between the mixing path inlet and a mixing path outlet. The low frequency blending mixer is in fluid communication with the outlet of the centrifugal mixing path.
    Type: Application
    Filed: July 15, 2024
    Publication date: November 7, 2024
    Applicant: Waters Technologies Corporation
    Inventor: Neal Almeida
  • Publication number: 20240367072
    Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with an immunoglobulin-binding protein. The functionalized particles of the present disclosure can be used to purify a range of immunoglobulins with affinity to the immunoglobulin-binding protein.
    Type: Application
    Filed: May 3, 2024
    Publication date: November 7, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Kevin Wyndham, Beatrice Muriithi, Oksana Tchoul, Yeliz Tunc Sarisozen, Mingcheng Xu, Matthew Lauber
  • Publication number: 20240359161
    Abstract: The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 micrometers and being functionalized with streptavidin. The streptavidin functionalized particles of the present disclosure can be bound to biotinylated antibodies, biotinylated antigen-binding fragments thereof, or biotinylated oligonucleotides to create customizable affinity chromatography columns. That is, the functionalized nonporous polymer particles can be packed in a stainless steel, titanium, or other metal or metal alloy column for affinity high pressure liquid chromatography (HPLC).
    Type: Application
    Filed: April 26, 2024
    Publication date: October 31, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Kevin Wyndham, Beatrice Muriithi, Oksana Tchoul, Yeliz Tunc Sarisozen, MingCheng Xu, Matthew Lauber
  • Publication number: 20240361318
    Abstract: The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).
    Type: Application
    Filed: April 26, 2024
    Publication date: October 31, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Kevin Wyndham, Beatrice Muriithi, Martin Gilar, Yeliz Tunc Sarisozen
  • Patent number: 12121829
    Abstract: The present disclosure relates to a filter. The filter includes a porous element, a compression element and a housing. At least a portion of the porous element is coated with an alkylsilyl coating. The compression element is configured to receive the porous element thereby forming an assembly. The housing has an opening formed therein. The opening is configured to receive the assembly. The assembly is retained within the opening when the assembly is received therein.
    Type: Grant
    Filed: February 25, 2020
    Date of Patent: October 22, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Mathew H. DeLano, Matthew A. Lauber, Michael Donegan, Robert A. Jencks, Stephen J. Shiner
  • Patent number: 12121891
    Abstract: Described herein are systems and methods used for washing needle assemblies with multiple needles.
    Type: Grant
    Filed: August 16, 2021
    Date of Patent: October 22, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Rose Solow, David A. Simpson, Colin F. Fredette, Marc E. Lemelin, Joshua A. Burnett
  • Publication number: 20240345037
    Abstract: Embodiments described herein relate to improved devices and techniques for measuring the charge carried by analyte particles. The charge may be measured in a way that does not destroy the analyte, so that the analyte remains available for further analysis. The charged analyte particles may pass through (or by) an electrode. In doing so, they induce a counter charge on that electrode which can be detected electronically. Subsequent to their passage through that electrode, the particles can be collected on a substrate or may pass in real-time into a mass spectrometer. In some embodiments, both conventional destructive detection and nondestructive detection may both be present in an improved charged aerosol detector and the stream of charged particles may be directed to one or the other by a suitable redirector. Embodiments may be combined with light scattering analysis to provide further non-destructive analysis. Various combinations of these improvements are also described.
    Type: Application
    Filed: April 12, 2024
    Publication date: October 17, 2024
    Applicant: Waters Technologies Corporation
    Inventor: Joseph A. Jarrell
  • Patent number: 12117445
    Abstract: The present disclosure relates to a method of separating a sample including a mycotoxin. The method includes flowing the sample through a column to load the sample on the column, washing the column to reduce impurities, eluting the mycotoxin from the column, collecting an eluted sample with the mycotoxin in a sample plate, sealing the eluted sample in the sample plate, transferring the sealed sample plate, removing the eluted sample from the transferred sample plate; and analyzing the removed eluted sample. The column can include an affinity resin, such as, for example, an immunoaffinity resin. The sample plate can be a 96-well plate.
    Type: Grant
    Filed: March 17, 2021
    Date of Patent: October 15, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Lingyun Chen, Jianmin Liu
  • Patent number: 12117427
    Abstract: The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.
    Type: Grant
    Filed: June 29, 2023
    Date of Patent: October 15, 2024
    Assignee: Waters Technologies Corporation
    Inventors: Kerri M. Smith, Paul Rainville
  • Publication number: 20240335830
    Abstract: A sample preparation device is provided for enriching a component of a sample. The sample preparation device includes a surface in fluid communication with the sample, an alkylsilyl coating disposed on the surface, and an affinity ligand or an enzyme covalently bonded to the alkylsilyl coating.
    Type: Application
    Filed: June 21, 2024
    Publication date: October 10, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Matthew A. Lauber, Mathew H. DeLano, Beatrice Muriithi, Anna Boardman
  • Publication number: 20240326019
    Abstract: The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.
    Type: Application
    Filed: June 11, 2024
    Publication date: October 3, 2024
    Applicant: Waters Technologies Corporation
    Inventors: Yeliz Tunc Sarisozen, Nicole L. Lawrence, Darryl W. Brousmiche, Andrew Wyatt Schmudlach, Matthew A. Lauber
  • Patent number: 12091433
    Abstract: In various aspects, the present disclosure pertains to sorbents for isolating at least one target protein from a liquid sample, the sorbents comprising a solid support comprising attached at least one attached high affinity reagent with an affinity for the at least one target protein. Other aspects of the present disclosure include kit that contain such sorbents and methods of treating samples using the same.
    Type: Grant
    Filed: September 24, 2021
    Date of Patent: September 17, 2024
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Laks Iyer, Beatrice W. Muriithi