Abstract: The present disclosure pertains to sample preparation devices useful for affinity capture and purification that include one or more internal structures that comprise a reservoir, a well, a fluid passageway, sorbent particles, and a filter element that blocks passage of the affinity sorbent particles, which sample preparation devices combine the attributes of both dispersive and flow through designs into a single sample preparation device. The present disclosure also pertains to kits that contain and methods that use such sample preparation devices.

Abstract: Provided herein are methods, techniques and processes for selecting one or more peptides for virus detection (e.g., influenza, SARS-CoV-2) in clinical samples using mass spectrometry. Methods for selecting a combination of peptides to identify one or more disease states in a patient can include: (i) selecting the one or more disease states; (ii) collecting information on proteins associated with the one or more disease states being present within the subject; (ii) in silico digesting individual proteins associated with the one or more disease state to obtain possible workflow peptides; (iv) collecting filtering data associated with the possible workflow peptides; (iv) analyzing the possible workflow peptides for coverage of the one or more disease states and for mass spectrometry detection and resolution; and (iv) selecting the combination of peptides from the possible workflow peptides based on the analyzing step.

Abstract: The present disclosure provides methods for the separation and quantitation of enantiomers of vitamin E using supercritical fluid chromatography (SFC) or carbon dioxide-based chromatography on chiral columns. The disclosed methods may be used to quantitatively determine the concentration of RRR-?-tocopherol in foods, food ingredients, dietary supplements, vitamin premixes, nutritional formulas, and medicines. Further provided is a method of differentiating the source of ?-tocopherol as natural or synthetic.

Abstract: An image charge detection assembly for a mass spectrometer comprising an image charge detector that is housed within an electrically conductive shielding enclosure, and a preamplifier, wherein at least an input stage of the preamplifier, which is electrically connected to the detector, is housed with the detector within the enclosure.

Abstract: The present disclosure is directed to surface modified materials such as stationary phase materials for performing size exclusion chromatography. Aspects of the present disclosure feature materials surface modified with a moiety including a polyethylene glycol (PEG) functionality and a moiety comprising a diol functionality. Such surface modified materials exhibit a reduced propensity for ionic and hydrophobic secondary interactions.

Abstract: There is provided a method of operating a charge detection mass spectrometer (CDMS), the CDMS comprising an electrostatic ion trap, the electrostatic ion trap comprising a plurality of electrodes, the method comprising: a) introducing a first ion into the electrostatic ion trap at a first ion energy, b) setting the voltage of the plurality of electrodes to a first voltage map, c) obtaining first CDMS data indicative of a first ion oscillation frequency, d) obtaining an acceptable range or ranges of ion oscillation frequencies, e) changing the first ion energy to a second ion energy and/or changing the first voltage map to a second voltage map, and f) obtaining second CDMS data indicative of a second ion oscillation frequency.

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are superficially porous chromatographic particulate materials comprising sized less than 2 microns.

Abstract: Described is a chain including a plurality of links pivotally connected to each other such that the chain is configured to bend in one direction without back-bending. Each of the plurality of links including a link body include: an inner side facing a bending direction of the chain; an outer side facing opposite the bending direction of the chain; a back-bending prevention portion; a post feature extending laterally across the link body, and a connection feature configured to engage with the post feature of the first other of the pivotally connected plurality of links to create one direction pivotal attachment without back-bending between the links. Further disclosed is a chain system and methods of moving devices with a chain or chain system.

Abstract: A chromatography system includes a gradient delay volume defined as an overall fluid volume between where gradient is proportioned until an inlet of a chromatography column, a pump pumping a flow of gradient; and at least one valve located downstream from the pump, the at least one valve having a plurality of ports including an inlet port that receives the flow of gradient from the pump and an outlet port through which the flow of gradient exits the at least one valve, the at least one valve having at least two positions. A first position of the at least two positions of the at least one valve increases the gradient delay volume of the chromatography system relative to when the at least one valve is in a second position.

Abstract: An actuator control system, mechanical testing system, and method for adaptive control of an actuator of a mechanical testing device is provided. The method includes applying a mechanical load to the specimen with the actuator, resulting in receiving a load sensor signal from a load sensor and a displacement sensor signal from a displacement sensor, deriving lumped dynamics of the mechanical testing device by analyzing a phase and magnitude relationship between a current command of a motor of the mechanical testing device and a resultant deflection of the specimen, and controlling the actuator based on the derived lumped dynamics of the mechanical testing device such that the controlling results in a stable motion.

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

Abstract: The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and/or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.

Abstract: The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30° C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.

Abstract: Provided herein chiral derivatization reagents for use in separating and detecting of amine containing enantiomers. The said chiral derivatization reagents provide a combination of improved detectable properties to facilitate various downstream analyses. In particular, the chiral derivatization reagents include at least one chiral carbon atom; at least one strongly basic moiety; at least one chromophore moiety or at least one fluorophore moiety; and at least one reactive group. The present disclosure further provides methods for analyzing amine-containing enantiomeric isomers using a chromatographic separation device and a mass spectroscopy.

Abstract: Methods for quenching a nuclease digestion of a target nucleic acid prior to downstream analysis of the target nucleic acid are disclosed herein. Particularly, methods for controlling the end point of a nuclease digestion prior to sequence analysis of a target nucleic acid is provided. Quenching of a nuclease digestion in the present disclosure employs at least one non-ionic or anionic denaturant combined with an optional reducing agent. The methods presented in this disclosure aids preserving the sample comprising the target nucleic acid or fragments thereof for long term storage and ensures that the effect of contaminating nucleases is eliminated during pretreatment step.

Abstract: A system capable of performing both single and multidimensional liquid chromatography includes a solvent delivery system, a sample injection system, a first dimension column path configured to perform a separation process in a first dimension, a second dimension column path configured to perform a separation process in a second dimension that is different than the first dimension, a valve system; and a sample injection system fluidically connected to the valve system. The valve system is configured to direct flow from a sample injection system to a first dimension column path when the valve system is in a first position, and to direct flow from the sample injection system to the second dimension column path without the flow path flowing through the first dimension column path in the chromatography system when the valve system is in a second position.

Abstract: In various embodiments, the present disclosure pertains to core-shell particles that comprise a porous hybrid organic-inorganic shell disposed on a surface-modified non-porous polymer particle core. In some embodiments, the present disclosure pertains to chromatographic separation devices that comprise such core-shell particles. In some embodiments, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such core-shell particles and (b) flowing a mobile phase through the column.

Abstract: An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in a fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry.

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.