Abstract: Temperature effects on results of a lateral flow assay can be compensated for by measuring an environmental temperature at a testing time and a testing location, performing a lateral flow assay at the testing time and testing location to obtain an original concentration value of a target substance, and generating a modified concentration value of the target substance. The modified concentration value is generated based on the original concentration value, by compensating for a difference between the environmental temperature and ambient temperature.

Abstract: Described are an interface module for two-dimensional chromatography and a method of performing a chromatographic separation that may use the interface module. The interface module includes a valve module, a collection needle, a modifier module and a sample storage element. The valve module has a first port configured to receive an eluent from a first chromatography system, a second port configured to provide a fraction obtained from the eluent, a third port and a fourth port. The collection needle and the modifier module are in fluidic communication with the valve module at the third and fourth ports, respectively. The modifier module includes a source of a modifier solvent. The sample storage element is in fluidic communication with the valve module and is configured to receive a volume of the fraction for injection as a sample into a second chromatography system.

Abstract: Methods for derivatization of biomolecules including glycans or other biopolymers with one or more fluorescent, MS active compounds by reductive amination or rapid tagging in order to produce derivatized glycan having a pKa>7 and between about 200 ?2 and about 1000 ?2 of nonpolar surface area are described.

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. In particular embodiments, the MS active, fluorescent molecules are of the compound of the Formula III or of the Formula IV where R1, R2 and R3 are defined herein.

Abstract: Techniques are described for accelerating thermal equilibrium in a chromatographic column. An apparatus comprises a chromatography column, and a plurality of temperature control units in thermal contact with the chromatography column. A method of performing liquid chromatography comprises setting an inlet of a chromatography column to a first temperature using a first temperature control unit in thermal contact with said inlet, setting an outlet of the chromatography column to a second temperature using a second temperature control unit in thermal contact with the outlet, wherein the first temperature is less than the second temperature; and injecting a sample into a liquid stream that flows through the chromatography column after the inlet is set at the first temperature and the outlet is at the second temperature.

Abstract: The present disclosure relates to systems and methods for determination of analyte concentrations in solution using materials that are pre-dispensed and dried on conjugate pads or other solid substrates. The solution including the analyte can reconstitute the dried material within a vial to produce a specified concentration of material in the vial. The resulting solution can be used in lateral flow testing.

Abstract: The present disclosure relates to methodologies, systems, and apparatus for controlling fluid flow within a chromatography system. The chromatography system includes a mobile phase pump configured to pump a liquid mobile phase through a column and a restrictor positioned downstream of the column and upstream of a detector. The system also includes a valve configured to operate in at least two positions. In a first position, the valve is configured to direct the output of the column to bypass the valve and reach the detector, while in the second position the valve directs the output of the column to waste.

Abstract: The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a metering device for drawing up the liquid sample, a first multi-port valve in fluid communication with a first end of the metering device and the liquid sample, a second multi-port valve in fluid communication with a second end of the metering device and a chromatography column, and a pump in fluid communication with the second multi-port valve and a mobile phase. When the valves are in a first position the metering device draws up the liquid sample filling a portion of the metering device. When the valves are in a second position, a remaining portion of the metering device is filled with the mobile phase thereby mixing with and pressurizing the liquid sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.

Abstract: The present disclosure generally relates to methods for determining chemical class compositions present in a sample using collision cross-section fragment ion values.

Abstract: A chromatography system includes a first chromatography column for receiving and separating a flow stream, a plurality of traps configured to trap a plurality of distinct flow segments exiting the first chromatography column during separation of the flow stream, and a second chromatography column operatively associated with the plurality of traps for receiving and separating the distinct flow segments. The system can include at-column dilution at trapping and separating stages thereof. A chromatography method for operating the chromatographic system includes measuring a plurality of time segments corresponding to a plurality of peaks of a fluid sample flowing through the first chromatographic column, and sequentially fluidly coupling the plurality of distinct flow segments with the corresponding plurality of traps during time segments corresponding to the plurality of peaks.

Abstract: A seal pack of a sample manager of a liquid chromatography system having a plurality of wash flow pathways fluidically connected to a central pathway that accommodates a sample needle, wherein a first wash flow pathway is vertically offset from a second wash flow pathway, such that a wash solution flows axially along an exterior surface of the sample needle in a vertical direction to wash the sample needle when flowing from the first wash flow pathway to the vertically offset second wash flow pathway, is provided. Furthermore, an autosampler and associated methods are also provided.

Abstract: A variable fluidic restrictor of a liquid chromatography system including a stator body, the stator body include a plurality of fluidic channels located within the stator body, wherein each fluidic channel of the plurality of fluidic channels includes a restrictor element, wherein a flow of a fluid through the variable fluidic restrictor is selectively restricted based on a position of an external element coupled to the stator body is provided. Furthermore, an associated method is also provided.

Abstract: Disclosed herein are chromatography columns with conical or reducing inside dimensions and improved fits for use therewith, along with methods for making the columns and the frits.

Abstract: The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

Abstract: The present disclosure relates to methods and workflows for processing and analyzing clinical samples (e.g., nasal swabs, throat swabs, plasma samples, urine, etc.) In general, the methods and workflows involve the detection of an infectious state and are improved over contemporary methods due to increased throughput with improved resolution of positive results.

Abstract: The disclosure relates to a reversed-phase chromatography method for the determination of at least one of glyphosate or AMPA in a sample. The method includes obtaining the sample comprising the at least one of glyphosate or AMPA in a sample matrix and introducing the sample to a reversed-phase chromatography system. The reversed-phase chromatography system includes a reversed-phase column having a stationary phase material contained inside the column. The stationary phase material includes a phenyl functional group. The method also includes flowing the sample with a mobile phase eluent through the reversed-phase column, wherein the at least one of glyphosate or AMPA is substantially resolved and retained. The mobile phase eluent has a pH between about 1.5 to about 2.5. The at least one of glyphosate or AMPA are detected using a detector.

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

Abstract: The disclosure relates to a recycling chromatography method that includes injecting a sample into a mobile phase flow stream of a chromatography system to create a combined flow stream. The sample includes an API and at least one impurity. The chromatography system includes a first column and a column in series, a first valve in fluid communication with the first and second chromatographic columns, a heater in communication with the first and second chromatographic columns, a fraction collector in fluid communication with the first and second chromatographic columns, and a second valve positioned before the fraction collector. The combined flow stream is recycled from the first chromatographic column to the second chromatographic column and vice versa by switching the first valve until a baseline resolution is achieved to separate the at least one impurity from the API. The at least one impurity is collected in the fraction collector.

Abstract: The present disclosure is directed to a kit for evaluating system inertness. The kit includes a positive control comprising a metal interacting moiety, and a negative control that does not contain a metal interacting moiety. In some embodiments the kit also includes a container to hold a system suitability solution (e.g., an equimolar mixture of the positive control and the negative control).

Abstract: The present disclosure relates to a method of reducing degradation of sample components in a liquid chromatography system. The method utilizes a masked metal frit to prevent or reduce metal surfaces from becoming catalytically active. The masked metal frit is a metal based frit that includes a coating on its exterior surfaces to mask or prevent contact between the organic solvents (and/or any analyte or other related solvent) and the underlying metal. The coating is a vapor deposited inorganic-organic hybrid coating, such as a vapor deposited C2 coating.