Abstract: This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyioin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds.

Abstract: Remote measuring and sensing. Some example embodiment related to optical energy harvesting by identification device, such as infrared identification device GRID devices). Other embodiments relate to RFID device localization using low frequency source signals. Yet still other embodiments related to energy harvesting by RFID in electric fields in both conductive and non-conductive environments.

Abstract: In one aspect, the present disclosure provides tubulysin analogs of the formula (I) wherein the variables are as defined herein. In another aspect, the present disclosure also provides methods of preparing the compounds disclosed herein. In another aspect, the present disclosure also provides pharmaceutical compositions and methods of use of the compounds disclosed herein. Additionally, drug conjugates with cell targeting moieties of the compounds are also provided.

Abstract: Provided herein are systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and cytosine deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided.

Abstract: In some embodiments, the present disclosure pertains to systems and methods for distilling a fluid by exposing the fluid to a porous membrane that includes a surface capable of generating heat. In some embodiments, the heat generated at the surface propagates the distilling of the fluid by converting the fluid to a vapor that flows through the porous membrane and condenses to a distillate. In some embodiments, the surface capable of generating heat is associated with a photo-thermal composition that generates the heat at the surface by converting light energy from a light source to thermal energy. In some embodiments, the photo-thermal composition includes, without limitation, noble metals, semiconducting materials, dielectric materials, carbon-based materials, composite materials, nanocomposite materials, nanoparticles, hydrophilic materials, polymers, fibers, meshes, fiber meshes, hydrogels, hydrogel meshes, nanomaterials, and combinations thereof.

Abstract: Laser-induced graphene (LIG) and laser-induced graphene scrolls (LIGS) materials and, more particularly to LIGS, methods of making LIGS (such as from polyimide (PI)), laser-induced removal of LIG and LIGS, and 3D printing of LIG and LIGS using a laminated object manufacturing (LOM) process.

Abstract: Disclosed herein methods for combating biofouling in a liquid, e.g. an aqueous medium by providing a surface coated with at least one laser-induced graphene (LIG) layer in said liquid medium. Particularly disclosed herein method and devices for treating water comprising passing a water stream through a membrane module equipped with at least one spacer coated with at least one layer of LIG, and optionally by applying an electric potential to the at least one LIG layer to achieve a bactericidal effect in the water stream. Specifically, disclosed herein a polymeric mesh suitable for use as a spacer in a membrane module in water treatment application, said mesh being at least partially coated with LIG.

Abstract: This invention describes a method of controlling the hybridization yield of nucleic acid probes by adjusting the relative concentrations of auxiliary oligonucleotides to the probes and the targets. The auxiliary oligonucleotide is partially or fully complementary to either the probe or the target, and is released upon hybridization of the probe to the target.

Abstract: In one aspect, the present disclosure provides disorazole analogs of the formula: Formula (I) wherein the variables are as defined herein. In another aspect, the present disclosure also provides methods of preparing the compounds disclosed herein. In another aspect, the present disclosure also provides pharmaceutical compositions and methods of use of the compounds disclosed herein. Additionally, drug conjugates with cell targeting moieties of the compounds are also provided.

Abstract: Laser-induced graphene (LIG) and laser-induced graphene scrolls (LIGS) materials and, more particularly to LIGS, methods of making LIGS (such as from polyimide (PI)), laser-induced removal of LIG and LIGS, and 3D printing of LIG and LIGS using a laminated object manufacturing (LOM) process.

Abstract: Provided herein are methods for encoding information in DNA molecules in a way that allows rapid and permanent erasure of information. As such, methods of erasing such information are also provided. Also provided are compositions that so encode information.

Abstract: The present disclosure provides methods for proximity-induced antibody conjugation of target agents).

Abstract: In some aspects, the present disclosure provides methods of aminating an aromatic compound comprising reacting an aminating agent with an aromatic compound in the presence of a rhodium catalyst. In some embodiments, the methods may comprise aminating an aromatic compound which contains multiple different functional groups. The methods described herein may also be used to create bicyclic system comprising reacting an intramolecular aminating agent with an aromatic ring to obtain a second ring containing a nitrogen atom. In another aspect, the methods described herein may also be used to create a cyclic aliphatic cyclic/poly cyclic amine system comprising a reacting an intramolecular aminating agent by insertion into a C(sp3)-H bond.

Abstract: A device, system, and methods are described to perform machine-learning camera-based indoor mobile positioning. The indoor mobile positioning may utilize inexact computing, wherein a small decrease in accuracy is used to obtain significant computational efficiency. Hence, the positioning may be performed using a smaller memory overhead at a faster rate and with lower energy cost than previous implementations. The positioning may not involve any communication (or data transfer) with any other device or the cloud, providing privacy and security to the device. A hashing-based image matching algorithm may be used which is cheaper, both in energy and computation cost, over existing state-of-the-art matching techniques. This significant reduction allows end-to-end computation to be performed locally on the mobile device.

Abstract: Real-world data may not be sparse in a fixed basis, and current high-performance recovery algorithms are slow to converge, which limits compressive sensing (CS) to either non-real-time applications or scenarios where massive back-end computing is available. Presented herein are embodiments for improving CS by developing a new signal recovery framework that uses a deep convolutional neural network (CNN) to learn the inverse transformation from measurement signals. When trained on a set of representative images, the network learns both a representation for the signals and an inverse map approximating a greedy or convex recovery algorithm. Implementations on real data indicate that some embodiments closely approximate the solution produced by state-of-the-art CS recovery algorithms, yet are hundreds of times faster in run time.

Abstract: Embodiments of the invention are directed to Ubx-fusion molecules that maintain their mechanical strength and properties even after being fused with Ubx. Ubx fusions with VEGF and other growth factors, cell signaling proteins, and cell binding proteins can be used to induce angiogenesis. Ubx fibers and mesh, embedded within a tissue engineering scaffold, induce formation of vasculature within the scaffold. The presence of vasculature is necessary to provide oxygen and nutrients to other cells growing within the scaffold.

Abstract: This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.

Abstract: Provide is a cement ink for a cement ink for 3D printing (which also includes additive manufacturing) of 3D cement structures and materials. The cement ink includes an American Petroleum Institute (API) Class G cement, a nano-clay, a superplasticizer, a hydroxyethyl cellulose, and a defoamer. The nano-clay may be hydrophilic bentonite. The superplasticizer may be a polycarboxylate ether. The defoamer may be 2-ethyl-1-hexanol. Processes for forming the cement ink and printing 3D cement structures using the cement ink are also provided.

Abstract: A multicomponent photocatalyst includes a reactive component optically, electronically, or thermally coupled to a plasmonic material. A method of performing a catalytic reaction includes loading a multicomponent photocatalyst including a reactive component optically, electronically, or thermally coupled to a plasmonic material into a reaction chamber; introducing molecular reactants into the reaction chamber; and illuminating the reaction chamber with a light source.

Abstract: Provided herein are reagents and methods for simultaneously enriching many potential rare genetic variants at different genetic loci. The rare variants enriched can include single nucleotide polymorphisms (SNPs), single nucleotide variants, or small insertions and deletions. Embodiments of the invention include procedures for integration with downstream next generation sequencing (NGS) analysis. Embodiments of the invention include analysis of nonpathogenic SNPs for the determination of cell identity and detection of cell contamination using qPCR or NGS.