Abstract: Disclosed are a gene mining method combining functional sequence and structure simulation, an NADH-preferring phosphinothricin dehydrogenase mutant and an application thereof. The gene mining method comprises the following steps: (1) analyzing a characteristic sequence which an NADH-type glutamate dehydrogenase should have; (2) searching a gene library based on the characteristic sequence; (3) performing clustering analysis and protein structure simulation on genes obtained by the searching; (4) selecting genes that feature high gene aggregation and a protein structure similar to that of the known phosphinothricin dehydrogenase as candidate genes. A wild-type phosphinothricin dehydrogenase with an amino acid sequence as set forth in SEQ ID No.

Abstract: A defense method against adversarial examples based on feature remapping, includes the following steps: building the feature remapping model, the feature remapping model is composed of the significant feature generation model and the nonsignificant feature generation model, and a shared discriminant model, the significant generation model is used to generate significant features, the nonsignificant generation model is used to generate nonsignificant features, and the shared discriminant model is used to discriminate fake or true of generated significant and nonsignificant features.

Abstract: Disclosed in the present invention is a rotary filtering type two-in-one stirring kettle. The stirring kettle includes a kettle body, where an upper sealing head and a lower sealing head are arranged at a top and a bottom of the kettle body respectively, a stirrer is arranged in the kettle body, and a rotary filtering mechanism is arranged between the kettle body and the lower sealing head. A back-flushing cleaning mechanism for dredging the rotary filtering mechanism is arranged at a bottom of the lower sealing head, and the rotary filtering mechanism is fixedly connected to the kettle body and the lower sealing head.

Abstract: Disclosed in the present invention are an energy-saving dishwasher and a control method therefor. The inner container is arranged inside the housing, the inner container is internally provided with a manifold type dish rack for placing dishes and spraying steam to perform steam cleaning on the dishes, and the manifold type dish rack is placed on a supporting frame fixed to the inner container. A water storage tank, a steam/hot water integrated generating device and a water pump are arranged below the inner container, and the steam/hot water integrated generating device includes a generating assembly and a control assembly. The manifold type dish rack is configured to place the dishes and simultaneously sprays steam to clean the dishes. The steam/hot water integrated generating device is configured to generate steam and hot water, heating is performed to generate the steam, and then relative cooling is performed to generate the hot water.

Abstract: The present invention discloses a method for building a co-culture model for Caco-2/RAW-264.7 cells induced by lipopolysaccharides and an application of the co-culture model. The method includes: 1) culturing Caco-2 cells and RAW-264.7 cells; 2) inoculating the 5 Caco-2 cells onto an AP side of an upper chamber of a Transwell plate, and incubating; 3) inoculating the RAW-264.7 cells onto a 24-well plate, and incubating; 4) transferring the upper chamber of the Transwell plate into the 24-well plate; 5) dissolving lipopolysaccharides in PBS to prepare a stock solution, filtering, and diluting for later use; and 6) adding an LPS-10% DMEM medium on a BL side, incubating, and forming an intestinal inflammation model.

Abstract: Disclosed is a method for predicting surface quality of a burnishing workpiece. The method includes the steps: using vibration sensors and signal acquisition instrument to acquire vibration signals generated on a surface of the burnishing workpiece during machining, evaluating the surface quality of the burnishing workpiece based on a coupling coordination degree model, processing signals by using an ensemble empirical mode decomposition method, identifying power spectral density, kurtosis and form factor as signal characteristics, identifying a support vector machine as a decision-making model, optimizing penalty parameters and kernel function parameters by using the Bayesian optimization method, and establishing the relationship between the signal characteristics and the surface quality.

Abstract: The present invention belongs to the field of printing, and specifically relates to a method for acquiring a color density characteristic curve in printing, including: designing a standard N-color level image of a single color channel; performing printout and scan correction on the standard N-color level image; performing edge finding operation on a corrected image; dividing the image into regions; and calculating a deviation. According to the method for acquiring a color density characteristic curve in printing by channels provided by the present invention, the color density characteristic curve of all the color channels may be obtained by sequentially using the method in the present invention on the color channels. The method in the present invention provides a basis for studying the performance of a printing system and further solving the problem of color density characteristic deviations at different positions.

Abstract: The present invention discloses a system for bridge scour multi-source monitoring, including an intelligent monitoring system, a data industrial personal computer, a 5G remote communication transmission system and a remote scour depth evaluation center. The intelligent monitoring system consists of three subsystems including a high-frequency real-time bridge dynamic characteristic monitoring system, an adjustable sound velocity underwater depth monitoring system and a scoured seabed soil pressure change testing system, which are coupled with one another to carry out triggering control to acquire lateral pile data, thereby forming a multi-source bridge local scour real-time sensing monitoring system, and realizing integration of clock synchronization, dynamic control of scour environment conditions, contact sensors and non-contact sensors.

Abstract: The present invention relates to a xylitol preparation device integrating evaporation, crystallization and centrifugation, including a xylitol tank, a cleaning liquid tank, a recycling tank and a multiple distribution system, wherein the multiple distribution system includes J groups of evaporators for evaporation concentration, K groups of vacuum crystallization kettles for vacuum crystallization and L groups of centrifuges for centrifugation, wherein 2?J?6, 6?K?12 and 2?L?4; the evaporator, the vacuum crystallization kettle and the centrifuge in different groups are sequentially connected in series with one another through a pipeline and a valve respectively; by controlling on and off of each valve, a xylitol exchange liquid is switched and controlled between a series-connection mode and a parallel-connection mode in the multiple distribution system to enable evaporation, crystallization and separation processes to reach an optimal effect distribution so as to improve productivity.

Abstract: The present disclosure discloses a digital hydraulic assisted braking system with a high dynamic response and a control method thereof. The system includes a two-position two-way digital switch valve A, a controller, an electromagnetic two-position two-way proportional reversing valve, a two-position two-way digital switch valve B, a displacement detection system, and a pressure detection system. When a vehicle starts, a control system of the present disclosure maintains a preload state and responds to a braking at any time. During an emergency braking, the braking system is open. When the vehicle is shut down, the braking system is closed. Compared with an existing braking method, by implementing the braking method of the present disclosure, a braking response time is greatly shortened, a dynamic performance of a braking response period is improved, and a safety of vehicle braking is further guaranteed.

Abstract: Disclosed is a method for predicting surface quality of a burnishing workpiece. The method includes the steps: using vibration sensors and signal acquisition instrument to acquire vibration signals generated on a surface of the burnishing workpiece during machining, evaluating the surface quality of the burnishing workpiece based on a coupling coordination degree model, processing signals by using an ensemble empirical mode decomposition method, identifying power spectral density, kurtosis and form factor as signal characteristics, identifying a support vector machine as a decision-making model, optimizing penalty parameters and kernel function parameters by using the Bayesian optimization method, and establishing the relationship between the signal characteristics and the surface quality.

Abstract: A triaxial test apparatus for measuring eroded soil particles under action of seepage force includes a constant-flow seepage system, a soil particle transport grading measurement system, a large-range volume pressure control vacuum system and a data processing and analyzing system, where the constant-flow seepage system is connected to the soil particle transport grading measurement system and the large-range volume pressure control vacuum system by means of pipelines separately, the data processing and analyzing system includes a data acquisition device and a computer, the constant-flow seepage system, the soil particle transport grading measurement system and the large-range volume pressure control vacuum system are connected to the data acquisition device by means of lines separately, and the computer is connected to the data acquisition device by means of a line.

Abstract: The present invention provides a polypeptide tag and its application in the synthesis of pharmaceutical chemicals, the recombinant nitrilase was obtained by connecting a polypeptide tag to the N-terminus of the amino acid sequence of the nitrilase; wherein amino acids at both ends of the polypeptide tag are uncharged glycine G, and the rest are a random combination of any one or more of glycine G, histidine H, glutamic acid E, aspartic acid D, lysine K and arginine R; The activity of the recombinant nitrilase in the preparation of 1-cyanocyclohexyl acetic acid is up to 3034.7 U/g dcw, the polypeptide tag significantly improves the soluble expression of nitrilase, and the whole cell catalyst hydrolyzes 1M substrate with the same concentration 30 minutes faster than the mother enzyme.

Abstract: The present disclosure discloses multi-mode hydraulic braking systems and control methods thereof. The system includes a two-position three-way digital switch valve, an electromagnetic three-position three-way proportional reversing valve, a controller, a pressure detection system, a speed detection system, and a displacement detection system. The controller intelligently switches a digital braking mode, a composite braking mode, and an anti-lock braking mode after collecting a brake signal through the pressure detection system and speed detection system in real time.

Abstract: Related to are a device and a method for performing continuous carbonation and impurity removal for xylose mother liquor.

Abstract: A method for rapid low-salted pickling of aquatic products is disclosed, which includes preliminary processing of raw materials, bacteria-reducing treatment of plasma activation gas, marinade adding and plasma treatment, cyclic bacteria-reducing treatment with the plasma activation gas and vacuum pickling. The marinade adding and plasma treatment include respectively injecting the marinade and plasma working gas into a tumbler, with a flow rate of ionic working gas injected into the tumbler of 1.5-2 L/min and a flow rate of a pickling solution of 0.5-0.8 L/min: and at the same time, starting a plasma power supply, and performing low-temperature plasma treatment on the aquatic products to be pickled, with a plasma treatment voltage of 12000-15000 V, a current of 60-80 mA, a frequency of 8-10 kHz, which is a second bacteria-reducing treatment, with plasma treatment time of 15-20 min.

Abstract: The present disclosure discloses a digital hydraulic assisted braking system with a high dynamic response and a control method thereof. The system includes a two-position two-way digital switch valve A, a controller, an electromagnetic two-position two-way proportional reversing valve, a two-position two-way digital switch valve B, a displacement detection system, and a pressure detection system. When a vehicle starts, a control system of the present disclosure maintains a preload state and responds to a braking at any time. During an emergency braking, the braking system is open. When the vehicle is shut down, the braking system is closed. Compared with an existing braking method, by implementing the braking method of the present disclosure, a braking response time is greatly shortened, a dynamic performance of a braking response period is improved, and a safety of vehicle braking is further guaranteed.

Abstract: Disclosed are a machine learning gene mining method and a phosphinothricin dehydrogenase mutant for amino translocation. The phosphinothricin dehydrogenase mutant for amino translocation is obtained by mutation of a wild-type phosphinothricin dehydrogenase with an amino acid sequence as shown in SEQ ID No.2 at one of the following sites: (1) E263D-K134R-H96A-R290V; (2) E263D-K134R-H96A; (3) E263D-K134R; (4) E263D; (5) E263N; (6) E263C; and (7) E263G. The present invention utilizes the site-saturation mutagenesis technology to mutate a phosphinothricin dehydrogenase gene as shown in SEQ ID No. 1, finds that the 263rd, 134th, 290th and 290th positions are the key sites affecting enzyme activity and stereoselectivity, and obtains a mutant with enzyme activity and ee value much higher than those of the parent phosphinothricin dehydrogenase.

Abstract: A method for extracting and purifying Dendrobium officinale polysaccharides comprises following steps: (1) fully disperse Dendrobium officinale powder in pure water to obtain crude liquid; (2) removing insoluble impurities from the crude liquid through a microfiltration membrane to obtain permeate 1 and retentate 1; (3) performing macroporous ultrafiltration treatment of the permeate 1 and collect permeate 2 and retentate 2; (4) adding an aqueous solution of edible alkali metal inorganic salt to the retentate 2, fully stirring and dissolving to obtain polysaccharide crude liquid, performing macroporous ultrafiltration treatment and collecting permeate 3 and retentate 3; (5) combining the permeate 2 and permeate 3, adding the combined permeate into an electrodialysis device for desalination, and collecting dilute solution and concentrated solution; (6) performing microporous ultrafiltration treatment of the dilute solution and collect retentate 4 and permeate 4; (7) carrying out freeze-drying of the retentate

Abstract: The present disclosure discloses multi-mode hydraulic braking systems and control methods thereof. The system includes a two-position three-way digital switch valve, an electromagnetic three-position three-way proportional reversing valve, a controller, a pressure detection system, a speed detection system, and a displacement detection system. The controller intelligently switches a digital braking mode, a composite braking mode, and an anti-lock braking mode after collecting a brake signal through the pressure detection system and speed detection system in real time.