Patents Examined by Amelia Burgess Yarbrough
  • Patent number: 5449602
    Abstract: Methods, apparatus and compositions are presented for ligating ligands together which bind to a common target. One embodiment includes polynucleotide probes having photoreactive functional groups. The probes are capable of assuming substantially contiguous reactive positions on a target polynucleotide placing the photoreactive groups in juxtaposition. Activation of the photoreactive functional groups with radiant energy form a probe reaction product in which the probes are bound to each other.
    Type: Grant
    Filed: January 13, 1988
    Date of Patent: September 12, 1995
    Assignee: Amoco Corporation
    Inventors: Garfield P. Royer, Larry E. Morrison, Kenneth A. Cruickshank
  • Patent number: 5411857
    Abstract: The invention relates to papillomaviruses, particularly to DNA-HPVs isolated from the papillomaviruses IP5 and IP6, the restriction maps of which are presented in FIGS. 10 and 11, and also to probes containing these DNA-HPVs or fragments obtained from them. The invention relates, in addition, to "kits" containing distinct groups of probes, containing one of these DNA-HPVs or DNA-HPV fragments, as well as a procedure for the detection and identification of papillomaviruses which makes use of these different probes.
    Type: Grant
    Filed: July 16, 1992
    Date of Patent: May 2, 1995
    Assignees: Institut Nationale de la Sante, Institut Pasteur
    Inventors: Sylvie Beaudenon, Dina Kremsdorf, Odile Croissant, Gerard Orth
  • Patent number: 5401629
    Abstract: Recombinant cells are provided which are useful for assaying compounds for their agonist or antagonist activity with respect to ion channels and/or cell surface-localized receptors. In addition, assay methods employing the invention recombinant cells are provided.
    Type: Grant
    Filed: August 7, 1990
    Date of Patent: March 28, 1995
    Assignee: The Salk Institute Biotechnology/Industrial Associates, Inc.
    Inventors: Michael M. Harpold, Paul Brust
  • Patent number: 5391723
    Abstract: There is disclosed an oligonucleotide strand or duplex-targeting protein conjugate, a linking group to link an oligonucleotide to a targeting protein, and a method for producing targeting protein conjugates containing oligonucleotide strands or duplexes. A suitable linker is a bifunctional phosphoramidite ester that binds to both the targeting protein and the oligonucleotide strand or duplex. The invention can be used for delivering chemotherapeutic agents to target sites based upon the specificity of the targeting protein and to deliver specific oligonucleotide sequences to a target site to hybridize and inhibit the expression of complementary sequences within the target cell genome.
    Type: Grant
    Filed: February 16, 1993
    Date of Patent: February 21, 1995
    Assignee: NeoRx Corporation
    Inventor: John H. Priest
  • Patent number: 5382512
    Abstract: The invention describes an assay device and assembly for detecting an analyte in a liquid sample. Each assay device in the assembly includes structure defining a well, a ligand-coated particle, and a flexible particle retaining structure for holding the particle in a captured position within the well.
    Type: Grant
    Filed: August 23, 1993
    Date of Patent: January 17, 1995
    Assignee: Chiron Corporation
    Inventors: Rick T. Smethers, Brian D. Warner
  • Patent number: 5380489
    Abstract: An element has been prepared which is useful for the detection of nucleic acids in various formats. The element has a sealable support on which is disposed a nucleic acid reagent composition. The composition is a mixture of a nucleic acid reagent composed of polymeric particles to which an oligonucleotide is covalently attached. The particles are prepared from a first polymer having a glass transition temperature of at least about 70.degree. C. and have an average diameter of from about 0.1 to about 3 micrometers. The reagent is adhered to the support using a water insoluble adhesive comprising a second polymer which has a glass transition temperature which is at least about 30.degree. C. less than the glass transition temperature of the first polymer. The adhesive is present in the composition at from about 1 to about 20 weight percent. This element provides high sensitivity and low background in hybridization and other nucleic acid assays.
    Type: Grant
    Filed: February 18, 1992
    Date of Patent: January 10, 1995
    Assignee: Eastman Kodak Company
    Inventors: Richard C. Sutton, Ignazio S. Ponticello, Thomas J. Cummins, Dennis R. Zander, William H. Donish
  • Patent number: 5376529
    Abstract: This invention relates to novel methods for the release of nucleic acids from cells in complex biological samples or specimens to prepare and make available the nucleic acid material present for a hybridization assay or for extraction. Novel methods for hybridization of nucleic acids are also presented. In particular methods are described for isolating nucleic acid from a sample containing a complex biological mixture of nucleic acid and non-nucleic acids wherein the sample is combined with a hybridization medium comprising a lactam which promotes and enables nucleic acid pairing when complementary nucleic acid is introduced. The lactam is preferably about 5 to about 70% of the hybridization medium and is most preferably 2-pyrrolidone, N-ethyl-2-pyrrolidone, N-cyclohexyl-2-pyrrolidone, N-dodecyl-2-pyrrolidone, N-methyl-2-pyrrolidone, N-hydroxyethyl-2-pyrrolidone, N-methyl-2-piperidone, 2-.epsilon.-caprolactam, N-methyl-2-caprolactam, 2-piperidone or N-(4-hydroxybenzyl)pyrrolidone.
    Type: Grant
    Filed: December 13, 1991
    Date of Patent: December 27, 1994
    Assignee: MicroProbe Corporation
    Inventors: Jeffrey Van Ness, Nicolaas M. J. Vermeulen
  • Patent number: 5376528
    Abstract: Improved nucleic acid probes capable of specifically hybridizing to rRNA of Listeria and not to rRNA of non-Listeria are described along with methods utilizing such probes for the detection of Listeria in food and other samples.
    Type: Grant
    Filed: February 19, 1993
    Date of Patent: December 27, 1994
    Assignee: Amoco Corporation
    Inventors: Walter King, Jyotsna S. Shah, Raymond M. Nietupski, Susan Raposa, Jane Warshaw, Patrick Groody, Jonathan Lawrie, George Parsons, Donald N. Halbert, David J. Lane
  • Patent number: 5370998
    Abstract: A novel composition and/or methods for the diagnosis of tuberculosis wherein the composition comprises a repetitive DNA segment that is specific for members of the Mycobacterium tuberculosis complex. The DNA segment repeats in the chromosome of Mycobacterium tuberculosis complex, and is conserved in all copies of the chromosomes. A method comprises using an entire repetitive DNA sequence, or any part thereof, as a hybridization probe for the direct detection of Mycobacterium tuberculosis complex in clinical material. In another method, a smaller portion of an entire repetitive DNA sequence is amplified using polymerase chain reaction, yielding a 123 base-pair product.
    Type: Grant
    Filed: October 1, 1991
    Date of Patent: December 6, 1994
    Assignee: The Board of Trustees of The University of Arkansas
    Inventors: Jack T. Crawford, Kathleen D. Eisenach, M. Donald Cave, Joseph H. Bates
  • Patent number: 5370991
    Abstract: A cloned gene encoding a new human elastase inhibitor is provided. The cloned gene is isolated, purified, and sequenced. The cloned gene encoding a human elastase inhibitor that is substantially non-glycosylated, is capable of forming a covalent complex with elastase and is capable of inhibiting elastase.
    Type: Grant
    Filed: September 6, 1991
    Date of Patent: December 6, 1994
    Assignee: The Center for Blood Research, Inc.
    Inventor: Eileen Remold-O'Donnell
  • Patent number: 5364760
    Abstract: Highly sensitive methods for assaying for biopolymers, such as by immunoassay or nucleic acid probe hybridization assay, and compositions for carrying out the methods, are provided. The methods employ as reporter group a RNA capable of being autocatalytically replicated by an RNA-dependent RNA polymerase, such as the replicase of bacteriophage Q.beta.. The high sensitivity of the assay methods is due to the rapid, exponential increase in concentration of such a replicative RNA, associated specifically with biopolymer analyte, that can be achieved by autocatalytic RNA replication.
    Type: Grant
    Filed: September 22, 1992
    Date of Patent: November 15, 1994
    Assignees: The Salk Institute for Biological Studies, The Trustees of Columbia University
    Inventors: Barbara Chu, Fred R. Kramer, Paul Lizardi, Leslie E. Orgel
  • Patent number: 5359044
    Abstract: Oligonucleotide surrogates comprising a plurality of cyclobutyl moieties covalently joined by linking moieties are prepared and used as antisense diagnostics and research reagents. Methods of synthesis and use of both the oligonucleotide surrogates and intermediates thereof are disclosed.
    Type: Grant
    Filed: December 13, 1991
    Date of Patent: October 25, 1994
    Assignees: ISIS Pharmaceuticals, Ciba-Geigy Ltd.
    Inventors: Phillip D. Cook, Gerhard Baschang
  • Patent number: 5359045
    Abstract: The invention relates to the cloning and sequencing of the nucleic acid coding for the human ACE as well as the determination of the peptide chain corresponding to the ACE and of active peptide fragments which derive from it.Another subject of the invention is the utilization of the above-mentioned polypeptides for the implementation of in vitro diagnostic methods for hypertension and for the design of new inhibitors of the ACE.The invention also relates to the utilization of the nucleotide probes of the invention for the in vitro screening of the polymorphisms of the gene coding for the ACE.
    Type: Grant
    Filed: May 23, 1990
    Date of Patent: October 25, 1994
    Assignee: Institut National de la Sante et de la Recherche Medicale
    Inventors: Florent Soubrier, Francois Alhenc-Gelas, Christine Hubert, Pierre Corvol
  • Patent number: 5356774
    Abstract: This invention relates to the use of functional reporter molecules in the detection and measurement of nucleic acid sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of a transcription step between the production of an appropriate reporter molecule and replication based amplification in order to increase the number of detectable species as an indirect reference to target nucleic acid sequence.
    Type: Grant
    Filed: July 2, 1992
    Date of Patent: October 18, 1994
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Vladimir D. Axelrod, Fred R. Kramer, Paul M. Lizardi, Donald R. Mills
  • Patent number: 5352579
    Abstract: This invention discloses hybridization assay probes specific for Histoplasma capsulatum and no other fungi.
    Type: Grant
    Filed: June 28, 1991
    Date of Patent: October 4, 1994
    Assignee: Gen Probe, Inc.
    Inventor: Curt L. Milliman
  • Patent number: 5348857
    Abstract: A method for detecting Brucella infection in an animal which is reliable, rapid, and able to identify species and biovars of Brucella. The detection method includes the amplification of the omp2 gene locus of Brucella and analysis of restriction digestion fragments specific to Brucella and to individual species and groups of biovars of Brucella.
    Type: Grant
    Filed: November 6, 1992
    Date of Patent: September 20, 1994
    Assignee: Texas A & M University
    Inventors: Thomas A. Ficht, L. Garry Adams
  • Patent number: 5346670
    Abstract: Red-shifted, water-soluble, fluorescent, monomerically-tetherable derivatives having the formula: ##STR1##wherein, M represents either H.sub.2 or is selected from among the following metals: aluminum, silicon, phosphorus, gallium, germanium, cadmium, scandium, magnesium, tin, and zinc. Each R.sub.1 is independently selected from --XYW, --YW, and --W. X represents either a carbon, or heteroatom selected from among oxygen, nitrogen, sulfur, phosphorus, silicon, and selenium; Y represents a linking group; and W represents a water solubilizing group. The substituent R.sub.2 is selected from among --A, --Y'A, --XA, and --XY'A, where A denotes a biological entity such as an antibody, antibody fragment, nucleotide, nucleic acid probe, antigen, oligonucleotide, deoxynucleotide, dideoxynucleotide, avidin, streptavidin or membrane probe, or R.sub.2 is a reactive or activatable group suitable for conjugating to a biological entity.
    Type: Grant
    Filed: June 8, 1992
    Date of Patent: September 13, 1994
    Assignee: British Technology Group U.S.A. Inc.
    Inventors: George E. Renzoni, Deborah C. Schindele, Louis J. Theodore, Clifford C. Leznoff, Karen L. Fearon, Barry V. Pepich
  • Patent number: 5334499
    Abstract: A nucleic acid is rapidly extracted from whole blood or a peripheral blood mononuclear cell (PBMC) fraction thereof. Extraction from the PBMC fraction is accomplished by heating the fraction at or near the boiling point of water for a few minutes and recovering the extracted nucleic acid. This rapid method is particularly useful for extracting DNA for the detection of genetic diseases or infectious agents, such as HIV-I. Whole blood can likewise be heated after it is mixed with a salt solution containing a polysaccharide, such as dextran. The extracted nucleic acid is then recovered from the heated mixture. Nucleic acids extracted in this way are available for amplification using a polymerase chain reaction. Where the presence of a specific gene is to be determined for diagnostic purposes, it can be extracted as described above and subjected to suitable amplification and detection steps.
    Type: Grant
    Filed: September 12, 1989
    Date of Patent: August 2, 1994
    Assignee: Eastman Kodak Company
    Inventors: Brent A. Burdick, Tobias D. Ekeze
  • Patent number: 5332666
    Abstract: A DNA sequencing system and method are described to detect the presence of radiant energy emitted from different excited reporter dye-labeled species (DNA fragments) following separation in time and/or space, and the identity of the species which emit radiant energy closely spaced in wavelength. Functions of the emitted energy are obtained which vary over the wavelengths of the closely spaced spectra in different senses and the functions ratioed, whereby the ratio is indicative of the identity of the DNA fragments. The emitting portion of the reporter-labeled DNA fragment is preferably one of a family of fluorescent dyes based on 9-carboxyethyl-6-hydroxy-3-oxo-3H-xanthene. These xanthene dyes are covalently attached to the DNA fragments through the carboxylic acid functionality, preferably via an amide linkage. The dyes may be protected by including an alkoxy group at the 9-position. A spacer may be inserted between the dye and the amine.
    Type: Grant
    Filed: October 22, 1991
    Date of Patent: July 26, 1994
    Assignee: E. I. Du Pont de Nemours and Company
    Inventors: James M. Prober, Rudy J. Dam, Charles W. Robertson, Jr., Frank W. Hobbs, Jr., George L. Trainor
  • Patent number: 5330900
    Abstract: Enzymatically cleavable chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature before a bond by which an enzymatically cleavable labile substituent thereof is intentionally cleaved, are disclosed. These compounds can be represented by the formula: ##STR1## wherein: X and X.sup.1 each represent, individually, hydrogen, a hydroxyl group, a halo substituent, an unsubstituted lower alkyl group, a hydroxy (lower) alkyl group, a halo (lower) alkyl group, a phenyl group, a halophenyl group, an alkoxyphenyl group, a hydroxyalkoxy group, a cyano group or an amide group, with at least one of X and X.sup.1 being other than hydrogen; andR.sub.1 and R.sub.2, individually or together, represent an organic substituent that does not interfere with the production of light when the dioxetane compound is enzymatically cleaved and that satisfies the valence of the dioxetane compound's 4-carbon atom, with the provisos that if R.sub.1 and R.sub.
    Type: Grant
    Filed: December 12, 1991
    Date of Patent: July 19, 1994