Abstract: A general method of assay for biological molecules using daylight fluorescent particles. The method described is applicable to assays involving immunological reagents, nucleic acids, hormones and neurotransmitters.

Abstract: Probe cocktails contain a nucleic acid probe and a sulfated polysaccharide as a volume excluding polymer to speed up hybridization rates. Exemplary sulfated polysaccharides are chondroitin sulfate A and C, which can be extracted from cartilage and similar tissues. Compared to synthetic anionic polymers such as dextran sulfate, the probe cocktails can have reduced viscosities and surface tensions, as well as reduced water loss when the cocktail is heated to denature DNA before rehybridization. The reduced viscosity and surface tension are of particular value when entering and exiting narrow spaces, as in some automated analyzers and in capillary gap methodologies.

Abstract: A paving composition comprising about 80 to 97% by weight of an open-graded aggregate and about 3 to 20% asphalt, said composition being formed by successively mixing two asphalt-containing emulsions A and B with said aggretate wherein:emulsion A comprises about 40 to 75% by weight of a soft asphalt having a viscosity in the range of 50 to 1000 centistokes at 210.degree. F. and 0.05 to 5% by weight of a emulsifier, and water as a continuous phase of said emulsion to make up 100% by weight; andemulsion B comprises about 40 to 75% by weight of a hard asphalt having a penetration 5 to 25 dmm at 77.degree. F. and 0.05 to 5% by weight of a emulsifier, and water as a continuous phase of said emulsion to make up 100% by weight.

Abstract: A method for the multiplex sequencing on DNA is disclosed which comprises the electroblotting or specific base terminated DNA fragments, which have been resolved by gel electrophoresis, onto the surface of a neutral non-aromatic polymeric microporous membrane exhibiting low background fluorescence which has been surface modified to contain amino groups. Polypropylene membranes are preferably and the introduction of amino groups is accomplished by subjecting the membrane to radio or microwave frequency plasma discharge in the presence of an aminating agent, preferably ammonia. The membrane, containing physically adsorbed DNA fragments on its surface after the electroblotting, is then treated with crosslinking means such as UV radiation or a glutaraldehyde spray to chemically bind the DNA fragments to the membrane through said smino groups contained on the surface thereof.

Abstract: Colloidal particles are converted into magnetic microagglomerates via manipulation of their colloidal properties, thereby facilitating their separation from solution.

Abstract: A method for determining and visualizing the base sequence of nucleic acid olymers, especially DNA and RNA, with a scanning probe microscope having a tip, the method including replacing the oxygen in the nucleic acid polymer with sulfur, complexing the sulfur with a metal such as mercury, and passing the tip over the complexed polymer in a scanning path to measure the potential and record the difference in electrical conductivity at preselected increments along the scanning path.

Abstract: Liquid bleaching and brightening compositions are provided in which a polymeric matrix stably suspends a fluorescent whitening agent, and, optionally, pigment particles. A particularly preferred composition includes an aqueous solution having sodium hypochlorite in an amount of from about 3.5 wt. % to about 6.2 wt. %, an anionic or nonionic surfactant in an amount of from about 0.03 wt. % to about 0.3 wt. %, polymer in an amount of from about 0.03 wt. % to about 2.0 wt. %, a fluorescent whitening agent in an amount of from about 0.01 wt. % to about 0.2 wt. %, and, if desired, ultramarine blue particles in an amount of from about 0.01 wt. % to 0.2 wt. %, the fluorescent whitening agent and ultramarine blue particles being stably suspended and displaced in the aqueous solution via the polymer.

Abstract: The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. A second oligonucleotide is present in the reaction that is homologous to the 3 prime end of the probe molecule and also conains 5 prime base or bases that will reconstitute the restriction enzyme site on the target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.

Abstract: There is disclosed a preservative composition which is ultra stable in the presence of wood extractives comprising (a) an emulsion of a wood preserative grade creosote; (b) 5-95% water; (c) one or more pre-dispersed micronized pigments; (d) a rheology structuring agent present in an amount of 2.5 weight percent or less; (e) 0.25 to 10 weight percent of a soap which is an alkali metal salt of a wood derived resin acid; (f) 0.1 to 5 weight percent of a surfactant; (g) 0.25 to 2 weight percent of a natural or synthetic pigment modifying resins or anti-settle additive; and (h) 0.25 to 5 weight percent of a lignin sulfonate. The emulsion is produced under conditions of ultra-high sheer. A process for the manufacture of the preservative composition is also disclosed.

Abstract: A method and kit for detecting a predetermined nucleotide sequence in a specimen using a nucleic acid probe modified with N-2-acetylaminofluorene (AAF).

Abstract: A distinctive repetitive genomic element of Leptospira interrogans serovar hardjo-type hardjo-bovis permits distinguishing this pathogen type from other commonly found leptospires in North American cattle using single-stranded RNA probes. Plasmids carrying DNA templates for useful probes have been deposited as NRRL Accession Nos. B-18462, B-18463, and B-18464. The probes are sufficiently sensitive to detect hardjo-bovis in as few as 1.times.10.sup.2 cells/ml. The diagnostic capabilities of the probes render them useful not only as herd management tools, but also in epidemiology studies designed to determine the origin and migration of L. interrogans isolates.

Abstract: The present invention provide transcriptional control regions, expression vectors comprising said regions, mammalian cells transformed to transcribe and express genes from said vectors, and various methods of assaying compounds for hormone agonist or antagonist activity, all based on discovery of response elements of the .beta.-retinoic acid receptor and of the activation of said response elements in all mammalian cells without need to transform the cells to express the receptor independently of endogenous expression thereof.

Abstract: An activated partial thromboplastin time (APTT) test is described which does not require blood which has been anticoagulated with citrate. The test enables the citrate anticoagulant step to be combined with the contact activation step and hence enables one to employ fresh non-anticoagulated blood specimens to directly perform the APTT Test.

Abstract: Nucleic acid fragment capable of hybridizing to rRNA of Listeria monocytogenes and not to rRNA of Bacillus subtilis.

Abstract: The assay of the subject invention uses DNA sequences as probes in a nucleic acid hybridization diffraction assay, to detect specific DNA sequences in a sample. Diffraction assay methodologies are applied to determine the presence and amount of analyte.This invention involves a discovery in the areas of supporting surfaces for a biogrid or biograting which provide greatly reduced non-specific hybridization and binding. A preferred process of this invention involves manufacturing a biograting for use in a light diffraction assay, and comprises adhering a uniform layer of hybridizing reagent comprising a nucleotide sequence on a smooth, solid surface and exposing the surface to UV radiation through a shadow mask with a diffraction grating pattern of lines to selectively deactivate the hybridizing reagent, leaving a biological diffraction grating design of lines of active hybridizing reagent.

Abstract: A monoazo lake pigment of the formula (I): ##STR1## whose X-ray diffraction pattern shows a high diffraction intensity at a diffraction angle (2.theta..+-.0.2.degree.; Cu - K.sub..alpha.) of 4.9.degree., moderate diffraction intensities at 18.4.degree., 25.9.degree. and 26.8.degree. and relatively low diffraction intensities at 11.1.degree., 15.3.degree. and 21.2.degree., and printing ink, a paint and plastics in which the monoazo lake pigment is contained.

Abstract: A general method of assay for biological molecules using daylight fluorescent particles. The method described is applicable to assays involving immunological reagents, nucleic acids, hormones and neurotransmitters.

Abstract: The invention relates to a process for the in vitro detection of a determined RNA, particularly of a mRNA connected with a genetic abnormality in a biological material. This process comprises a treatment of the cells contained in that material for the sake of releasing their cellular components and of exposing the RNAs yet without denaturing other nucleic acids, and then the detection operation comprising contacting RNA sought to be detected, in presence of the other cellular components, with a nucleotidic sequence complementary of the RNA sought.

Abstract: A water-based ink composition for a ball-point pen comprises: a colorant selected from dyes or pigments; a water-soluble organic solvent; water; and a metal salt and/or an amine salt of N-acyl sarcosine. This ink can effectively prevent the wear of a ball seat of the pen caused by the rotation of a ball of the same to make it possible to keep good ink-discharging properties of the pen, whereby a smooth writing of the pen can be performed.

Abstract: Metallization, applied by the thick film screening technique, utilized herein has glass-ceramic bonding agents designed to promote adhesion yet maintain the desired electrical properties and post-processing characteristics.