Patents Examined by Ann Lam
  • Patent number: 9944981
    Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: April 17, 2018
    Inventors: Jonathan Rothberg, Wolfgang Hinz, John Davidson, Antoine van Oijen, John Leamon, Martin Huber
  • Patent number: 9933428
    Abstract: An immunoassay test slide for use in a dry chemistry analytical instrument includes a slide housing or case formed from two matable sections—a slide cover piece and a slide bottom piece. The slide housing defines an interior cavity in which is situated a sheet-like porous carrier matrix. The slide cover piece has an opening formed through the thickness thereof to expose a central portion of the fluid flow matrix so that a precise volume of fluid sample of blood, serum or the like, preferably pre-mixed with a conjugate reagent, and precise volumes of a wash reagent and a substrate (detector reagent), may be deposited on the matrix through the cover opening by a metering device of the analytical instrument. The bottom piece of the immunoassay test slide is transparent, and the slide is moved by a transport mechanism of the analytical instrument over a reflectometer or a fluorometer for performing reflectance or fluorescence measurements.
    Type: Grant
    Filed: January 7, 2013
    Date of Patent: April 3, 2018
    Assignee: IDEXX Laboratories, Inc.
    Inventors: Eugene Chan, Keith Nassif
  • Patent number: 9888932
    Abstract: A device for delivery of material or stimulus to targets within a body to produce a desired response, the targets being at least one of cells of interest, cell organelles of interest and cell nuclei of interest. The device includes a number of projections for penetrating a body surface, with the number of projections being selected to produce a desired response, and the number being at least 500. A spacing between projections is also at least partially determined based on an arrangement of the targets within the body.
    Type: Grant
    Filed: October 3, 2011
    Date of Patent: February 13, 2018
    Inventor: Mark Anthony Fernance Kendall
  • Patent number: 9890420
    Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.
    Type: Grant
    Filed: July 25, 2016
    Date of Patent: February 13, 2018
    Assignee: Duke University
    Inventor: Ashutosh Chilkoti
  • Patent number: 9891230
    Abstract: The present invention relates to a microarray device for the screening or the finding of protein inhibitors, to a method for the production thereof, and to a corresponding method for screening or finding protein inhibitors. The microarray device according to the invention comprises a solid supporting element having a support material, at least one protein immobilized thereon for which inhibitors are to be screened or found, and at least one known inhibitor of the at least one protein, the inhibitor being bound to the at least one protein and comprising a detectable label.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: February 13, 2018
    Inventors: Dieter Melzner, Thomas Scheper, Thomas Schueler, Frank Stahl, Denise Van Rossum, Johanna-Gabriela Walter, Carsten Zeilinger
  • Patent number: 9879360
    Abstract: Disclosed herein is a microfluidic device comprising a chip; a flow channel being disposed in the chip; the flow channel being in communication with an entry port and an exit port; the flow channel being operative to permit the flow of a library from the entry port to the exit port; a substrate; the substrate being disposed upon the chip; the substrate being operative to act as an upper wall for the flow channels; and a plurality of receptors; the plurality of receptors being disposed on the substrate; the plurality of receptors being operative to interact with an element from the library.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: January 30, 2018
    Inventors: Emmanuel Delamarche, Robert Lovchik, Daniel J. Solis
  • Patent number: 9857367
    Abstract: A sample plate comprising a sample well is disclosed. The sample well can comprise one or more bead retaining chambers. Also provided herein is a method of using the sample plate and kits comprising the sample plate.
    Type: Grant
    Filed: September 12, 2013
    Date of Patent: January 2, 2018
    Assignee: Dynex Technologies, Inc.
    Inventors: Adrian Bunce, Andrew Fusellier
  • Patent number: 9851365
    Abstract: Methods and devices for liquid-liquid extraction using digital microfluidic arrays are provided. A polar droplet is transported to a separation region containing a substantially non-polar solvent, where non-polar impurities may be extracted from the polar droplet while maintaining a distinct phase separation. In a preferred embodiment, biological samples containing hormones are dried on a digital microfluidic array, lysed by a lysing solvent, dried, subsequently dissolved in a polar solvent, and further purified in a separation step in which droplets are transported through a volume of non-polar solvent. The method disclosed herein provides the distinct advantage of an automated sample preparation method that is capable of extracting hormones from low sample volumes with high precision and recovery.
    Type: Grant
    Filed: February 26, 2010
    Date of Patent: December 26, 2017
    Inventors: Noha Ahmed Mousa, Mais J. Jebrail, Mohamed Omar Abdelgawad, Aaron R. Wheeler, Robert Fredric Joseph Casper
  • Patent number: 9846155
    Abstract: Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.
    Type: Grant
    Filed: August 17, 2015
    Date of Patent: December 19, 2017
    Assignee: Quanterix Corporation
    Inventors: David M. Rissin, David Fournier, David C. Duffy
  • Patent number: 9841422
    Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: December 12, 2017
    Assignee: Accelerate Diagnostics, Inc.
    Inventors: David A. Goldberg, David C. Howson, Steven W. Metzger, Daniel A. Buttry, Steven Scott Saavedra
  • Patent number: 9802374
    Abstract: A method of manufacturing a biopolymer sensor including providing a biopolymer, processing the biopolymer to yield a biopolymer matrix solution, adding a biological material in the biopolymer matrix, providing a substrate, casting the matrix solution on the substrate, and drying the biopolymer matrix solution to form a solidified biopolymer sensor on the substrate. A biopolymer sensor is also provided that includes a solidified biopolymer film with an embedded biological material.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: October 31, 2017
    Inventors: David L. Kaplan, Fiorenzo Omenetto, Brian Lawrence, Mark Cronin-Golomb, Irene Georgakoudi
  • Patent number: 9797843
    Abstract: The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal assay format.
    Type: Grant
    Filed: July 28, 2015
    Date of Patent: October 24, 2017
    Inventors: Nicholas Abbott, Christopher Murphy, Barbara Israel
  • Patent number: 9797895
    Abstract: The present invention relates to the use of a composition comprising (a) at least three different amino acids, (b) at least two different amino acids and a saponin or (c) at least one dipeptide or tripeptide for stabilizing biomolecules immobilized on a solid carrier. The invention furthermore relates to a method for producing stabilized biomolecules, comprising embedding the biomolecules in the composition according to the invention and a method of producing a solid carrier having biomolecules attached thereto. The invention furthermore relates to a solid carrier producible or produced by the method of the invention and a method of diagnosing a disease using the carrier of the invention.
    Type: Grant
    Filed: March 31, 2010
    Date of Patent: October 24, 2017
    Assignee: Leukocare AG
    Inventors: Stefan Margraf, Anja Breuer, Martin Scholz, Jens Altrichter
  • Patent number: 9778223
    Abstract: A separation module operates to fractionate or separate an analyte into fractions according to pI, i.e., pI bands, utilizing capillary isoelectric focusing (“CIEF”) within a first microchannel. The fractions are stacked to form plugs, the number of which is determined by a number of parallel second microchannels integrally connected to the first microchannel, into which the fractions are directed according to the buffer characteristics found in each of the individual microchannels. Within the microchannels the plugs are separated into proteins according to a different chemical property, i.e., “m/z,” utilizing capillary electrophoresis (“CE”).
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: October 3, 2017
    Assignee: Leidos, Inc.
    Inventors: Thomas Wayne Schneider, James N. Baraniuk
  • Patent number: 9739771
    Abstract: The present invention provides a microfluidic purification chip for capturing a biomarker from a physiological solution. The present invention also provides a method of capturing and releasing a biomarker, wherein the biomarker is originally in a physiological solution. The present invention further provides a method of pre-purifying and measuring the concentration of a biomarker in a physiological solution.
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: August 22, 2017
    Inventors: Tarek M. Fahmy, Eric D. Stern, Mark A. Reed
  • Patent number: 9733165
    Abstract: Embodiments in accordance with the present invention relate to methods and apparatuses for concentrating and isolating Circulating Tumor Cells (CTCs) from body fluids. One embodiment of the present invention includes a micro-fabricated or nano-fabricated device having channels configured for separating and excluding. Embodiments in accordance with the present invention utilize features that reduce the hydrodynamic pressure experienced by the cells during the separation, isolation and concentration processes, and therefore reduce the likelihood of cell lysis or other damage to the cells.
    Type: Grant
    Filed: January 31, 2014
    Date of Patent: August 15, 2017
    Assignee: University of Washington
    Inventors: Daniel T. Chiu, Jason S. Kuo
  • Patent number: 9733166
    Abstract: A tissue embedder comprising a transport mechanism for an input member, the input member adapted to hold a plurality of tissue supports, with each tissue support associated with a mould, a wax bath and a cooling station wherein the transport mechanism moves the input member from a wax bath to a cooling station.
    Type: Grant
    Filed: December 18, 2007
    Date of Patent: August 15, 2017
    Inventors: Eduardo Vom, Chester John Henderson, Craig Matthew Lewis
  • Patent number: 9719895
    Abstract: Contemplated herein is an automated microscope slide antigen recovery and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments for individually and independently processing a plurality of individual microscope slides. The apparatus preferably features independently movable slide support elements each having an individually heatable heating plate. Each slide support element may support a microscope slide. Each microscope slide can be enclosed within an individual pressurizable reaction compartment. Pressures exceeding 1 atm or below 1 atm can be created and maintained in the reaction compartment prior to, during or after heating of the slide begins.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: August 1, 2017
    Inventor: Lee H. Angros
  • Patent number: 9707333
    Abstract: The invention described herein teaches methods of removing microvesicular particles, which include but are not limited to exosomes, from the systemic circulation of a subject in need thereof with the goal of reversing antigen-specific and antigen-nonspecific immune suppression. Said microvesicular particles could be generated by host cells that have been reprogrammed by neoplastic tissue, or the neoplastic tissue itself. Compositions of matter, medical devices, and novel utilities of existing medical devices are disclosed.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: July 18, 2017
    Assignee: Aethlon Medical, Inc.
    Inventors: Thomas Ichim, Richard H. Tullis
  • Patent number: 9696276
    Abstract: Disclosed are methods and apparatus for separation of biomolecules via two-dimensional gel electrophoresis, methods and apparatus for immunoblotting separated biomolecules, and methods for the use of biomolecules processed via the methods and apparatus of the present invention, including use in a clinical setting. The methods and apparatus for separation of biomolecules via two-dimensional gel comprises vertical agarose gel electrophoresis in the first dimension, and the electrophoresis of a novel non-denaturing 3-35% concave gradient polyacrylamide gel in the second dimension. This novel gel can be cast in a modified gel caster that can facilitate the pouring of multiple gels simultaneously. The methods and apparatus for immunoblotting are useful with any type of immunoblotting, including Western blot, Northern blot, and Southern blot analyzes.
    Type: Grant
    Filed: May 17, 2013
    Date of Patent: July 4, 2017
    Assignee: Boston Heart Diagnostics Corporation
    Inventors: Bela F. Asztalos, Ernst J. Schaefer