Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Abstract: Provided are technologies capable of direct measurement of activity of a bioactive substance in cell using nanowires, more particularly, a method for measuring intracellular activity of a bioactive substance using a nanowire support to which cells are immobilized and a nanowire support to which target substances for the subject bioactive substance are immobilized, and a chip for measuring intracellular activity of a bioactive substance including nanowires to which cells are immobilized and nanowires to which a target substance for the subject bioactive substance is immobilized.

Abstract: A device for biochemical processing and analysis of a measured sample volume of a sample is described. The device is characterized in that it consists of a sealed vessel (1) and that it comprises at least one thin pierceable membrane (2) through which a capillary tube (3) containing said measured sample volume of a sample can pass into said sealed vessel (1). Said sealed vessel (1) further contains at least one biochemically reactive substance (4) and a liquid (6). A method, wherein the device according to the invention is used for analysis, is also described.

Abstract: An immunodetection probe comprises a needle structure having a compartment and configured to be inserted into an organic tissue, a dialysis membrane configured to isolate the compartment from the organic tissue, a detection device having a detection portion and a plurality of receptors, a first optical fiber coupled to the needle structure, and a pair of tubes connected to the compartment. The plurality of receptors are disposed on an end surface of the detection portion for conjugating target antibodies, wherein the detection portion is disposed in the compartment. The first optical fiber is configured to introduce light incident on photo-induced molecules adjacent to the end surface of the detection portion so as to cause a change in the pH level of the solution adjacent to the end surface of the detection portion. The pair of tubes is configured to transport the solution containing the photo-induced molecules into the compartment.

Abstract: A first permeable mesh membrane may be configured to be tautly stretched over a tissue sample and overlaid over a surface. A first permeable mesh membrane may sandwich and secure a tissue sample in between a first permeable mesh membrane and a surface. A first permeable mesh membrane may have a sieve size of approximately 10 ?m to approximately 100 ?m.

Abstract: A method and apparatus for identifying an object having a pattern of plasmon resonant particles (PREs) distributed in or on the object are disclosed. In the method, a field containing the pattern is illuminated, and one or more spectral emission characteristics of the light-scattering particles in the field are detected. From this data, an image of positions and spectral characteristic values in the field is constructed, allowing PREs with a selected spectral signature to be discriminated from other light-scattering entities, to provide information about the field. The image may be compared to a database of reference images to identify or validate the object.

Abstract: The present invention relates to a method and a device for small scale reactions, such as sample preparation of a desired substance in a sample. In the method using the device samples mixed with functionalized magnetic particles are magnetically transferred between different working stations on the device. The method uses a hydrophobic surface, such as a Petri dish, provided with hydrophilic spots of, for example, agarose beads located on said hydrophobic surface and provided with buffers, reactants or ligands.

Abstract: Provided is a microbead analysis method for a microbead. The microbead is formed in a columnar shape having a top surface and a bottom surface facing each other, as placed almost in parallel, and a side surface extending therefrom, and carries an identification pattern formed on at least one of the top surface and the bottom surface and a substance immobilized on a surface thereof having affinity to an analyte substance. The method includes detecting fluorescence emitted from the microbead surface due to interaction of the analyte substance with the substance having affinity to the analyte substance from a region including a region of the top surface and the bottom surface where there is no identification pattern formed and the side surface.

Abstract: The present invention discloses systems and methods for minimizing or eliminating steps in immunosorbent assays for single and multiple analytes by eliminating both the need to attach target molecules to the test well and the need to remove unbound antibodies through rinsing. The immunosorbent assay (ISA) is utilized for a single analyte or target and includes the step of mixing the immunologic molecules with the sample and detection. The present invention further discloses an immunosorbent assay for multiple analytes (ISAMA) for testing a plurality of analytes or targets in a single well using a modified ISA test wherein different tags are attached to different antibody pairs. Alternate embodiments use multiple types of scavenger antigens with corresponding elimination of the need for scavenger antibodies. The present invention discloses various types of test wells for the rapid and simultaneous testing of fluids for a plurality of components and a methodology for a continuous immunosorbent assay.

Abstract: The invention is an electronically processed single-step test device for detecting the presence of a preselected analyte in a fluid. The device includes a hollow rectangular outer casing, disposed within co-joined upper and lower sections of the casing are assay material, an electronic processing system, and a LCD display. The LCD display is observable through a viewing window. The assay material is a sorptive material including a fluid sample application region in the form of a sample wick in fluid communication with a test strip. The test strip includes an analyte capture region adjacent to a light shield. The electronic processing system includes red and green LEDs which are alternately pulsed or energized over predetermined periods of time to determine if fluid test results show a marker or markers in the capture region indicative of the presence of a preselected analyte in the fluid. If so, Yes+ is displayed on the LCD. If not, No? is displayed on the LCD.

Abstract: A method of analysis or diagnosis of solid objects is based on real-time detection of how predefined probes interact with structures present on or in the solid object combined with the calculation of how the recorded binding curves of said probes are distributed in terms of interaction properties. The interaction properties are input to a classification algorithm which automatically determines statues of the solid object. The method is particularly advantageous for solid biological objects like tissue slices combined with antibody probes, said antibody recognizing receptors known to be over-expressed in disease states on said tissue slice.

Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Abstract: For the purpose of improving the accuracy of a variety analyses using a bio-microarray, it is a main object of the invention to provide a bio-microarray capable of producing relatively high signal intensity from a fluorescent molecule and capable of having improved quantification performance and to provide a substrate for bio-microarray. In order to achieve the object, the invention provides a substrate for bio-microarray characterized by having a reflection-suppressing function.

Abstract: Embodiments in accordance with the present invention relate to methods and apparatuses for concentrating and isolating Circulating Tumor Cells (CTCs) from body fluids. One embodiment of the present invention includes a micro-fabricated or nano-fabricated device having channels configured for separating and excluding. Embodiments in accordance with the present invention utilize features that reduce the hydrodynamic pressure experienced by the cells during the separation, isolation and concentration processes, and therefore reduce the likelihood of cell lysis or other damage to the cells.

Abstract: A portable sampling device (2) for obtaining a biological sample from a subject comprises sample collection means (8) for excising a biological sample (14) from a subject, and sample containment means (6) for containing excised sample (14) and sample preservative (12) for preserving excised sample. The sample collection means (8) includes sample cutting means (18) adapted, inuse, to cut into the subject and release the biological sample therefrom.

Abstract: Disclosed are an article for assaying a target, wherein the article includes a solid surface on which a first binding member, a blocking material, and a second binding member are immobilized, a method of manufacturing the article, and a method of detecting a target using the article.

Abstract: The present invention relates to methods of making nanoarrays for use in detecting species formed on the surface of the array using SE(R)RS. The methods can involve nanolithographic printing of a compound by dip pen nanolithographic printing. A SE(R)RS substrate can be used for the array and which can be selected from surfaces roughened by the oxidation-reduction cycle (ORC), island films, colloidal nanoparticles and surface-confined nanostructures. A coating or intermediate layer, such as a layer formed of nitrocellulose, can be provided between the compound and the SE(R)RS substrate. There are also provided arrays themselves and methods of using such arrays.

Abstract: An analyte measurement system is provided having sensors with embossed test chamber channels. In one embodiment, the sensors are elongate test strips for in vitro testing, each test strip having a substrate, at least one electrode, an embossed channel in the electrode, and lidding tape covering at least a portion of the embossed channel. Methods of manufacture are also disclosed for filling the sensor channels with reagent, and for trimming the ends of the sensors to eliminate the need for a calibration code during use of the sensors with a meter.

Abstract: A device including a sample compartment, a coil and an arm for mechanical manipulation of a sample vessel placed in the sample compartment and containing a sample is described. In at least one embodiment, the coil is surrounding the sample compartment and the sample compartment has an opening for insertion and removal of the sample vessel. A method, using the device according to at least one embodiment of the invention for detection of magnetic permeability, relative magnetic permeability or relative magnetic susceptibility, is also described.

Abstract: Elongated molecules are stretched across a substrate by controlled fluid flow.