Abstract: An observation method of a sample containing a target substance, the observation method including an imaging step in which a step of obtaining a speckle image including, as a speckle, light emitted from a luminescent substance in which a medium is brought into contact with the sample is performed a plurality of times so as to obtain a plurality of speckle images, the medium containing a probe that contains the luminescent substance emitting light and that repeatedly binds to and dissociates from the target substance directly and specifically, and an observation image generation step of generating an observation image of the target substance in the sample from the plurality of speckle images, wherein a half-life of a probe-target complex formed by binding between the probe and the target substance is equal to or more than 10 milliseconds and equal to or less than 3 seconds.

Abstract: An analyte-sensitive substance is provided that has an optical property related to the concentration of an analyte. The analyte-sensitive substance includes an ionophore or other substance configured to provide a local pH, within the analyte-sensitive substance, that is related to the concentration of the analyte proximate the analyte-sensitive substance. The analyte-sensitive substance further includes a pH-sensitive fluorophore that increases or decreases its intrinsic fluorescence intensity with the local pH across a specified range of pH values. The analyte-sensitive substance further includes a pH-sensitive quencher configured to increase the slope of the change of fluorescence intensity of the pH-sensitive fluorophore across the specified range of pH values. The analyte-sensitive substance may further include an ionic additive configured to adjust the local pH such that the specified range of pH values corresponds to a range of analyte concentration values of interest.

Abstract: A multi-unit for performing a biochemical test and immunological response test and a test method using the same comprising an upper strip composed of a plurality of first strip wells including an indicator for the biochemical test; a lower strip composed of a plurality of second strip wells including DNA fragments or antibodies for the immunological response test; and plasma which reacts with the indicator, DNA fragments, or antibodies, and the strip enclosure composed of a plurality of third strip wells in which the upper strip and the lower strip are sequentially inserted and coupled. The bottom surface of the first strip well and the bottom surface of the second strip well are spaced apart from each other by a predetermined distance.

Abstract: Provided is a multi-flux micro-fluidic chip including a chip body. The chip body includes a fluid inflow cavity communicated with an external air path, reaction-quantification cavities, waste liquid cavities, and a fluid path distribution cavity disposed at a middle position of the chip body. The two or more reaction-quantification cavities are distributed on two sides of the fluid path distribution cavity in rows to form the first and second row of reaction-quantification cavities respectively; and they are communicated with a fluid outlet of the fluid path distribution cavity through fluid path branches, and a fluid inlet of the fluid path distribution cavity through fluid path branches, and a fluid inlet of the fluid path distribution cavity is communicated with a fluid outlet of the fluid inflow cavity and an external fluid path, which making it possible to detect multiple items simultaneously and greatly improving the flux of the micro-fluidic chip.

Abstract: A device for delivery of material or stimulus to targets within a body to produce a desired response, the targets being at least one of cells of interest, cell organelles of interest and cell nuclei of interest. The device includes a number of projections for penetrating a body surface, with the number of projections being selected to produce a desired response, and the number being at least 500. A spacing between projections is also at least partially determined based on an arrangement of the targets within the body.

Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Abstract: The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise. The present invention is also directed to an electrochemiluminescent detection system for measuring a chemiluminescent signal on a probe tip.

Abstract: Contemplated herein is an automated microscope slide antigen recovery and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments for individually and independently processing a plurality of individual microscope slides. The apparatus preferably features independently movable slide support elements each having an individually heatable heating plate. Each slide support element may support a microscope slide. Each microscope slide can be enclosed within an individual pressurizable reaction compartment. Pressures exceeding 1 atm or below 1 atm can be created and maintained in the reaction compartment prior to, during or after heating of the slide begins.

Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Abstract: Devices, systems and methods for detecting molecules in a solid state using UV lights in a dry condition are provided. Interaction between a plurality of molecules, a conformational change of a molecule, a size difference between a plurality of molecules and a presence of a molecule can be detected in a dry environment by measuring a light absorption in at least one of light transmission mode and light reflection mode. In a preferred embodiment, the measurement of a light absorption is performed at a wavelength of ultraviolet light between 260 nm and 285 nm.

Abstract: Provided is a single-channel chemiluminescent micro-fluidic chip, including a chip body with a quantification-reaction cavity and a waste liquid cavity. The quantification-reaction cavity is composed of a quantification-reaction pool on a lower portion and a reaction pool cover plate, the quantification-reaction pool is equally divided into three cavities by two partition plates, a labeled antibody is placed in the middle cavity, coated antibodies are placed in the others, a surface, facing the quantification-reaction pool, of the reaction pool cover plate is equally divided into two parts. In result, the coated antibodies and the labeled antibodies are physically separated to effectively avoid nonspecific binding. Due to a special structure of the reaction tank, wavy fluid flow is generated to fully mix the coated antibodies and the labeled antibodies, which improves testing efficiency and sensitivity.

Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Abstract: In one aspect, nanoplasmonic devices are described herein. In some embodiments, a nanoplasmonic device comprises a radiation transmissive substrate, a metal layer positioned on the substrate and at least one aperture extending through the metal layer to the radiation transmissive substrate, wherein width of the aperture decreases with increasing depth of the aperture.

Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Abstract: Disclosed is an analysis method including: moving a complex, by means of a magnet unit, in a flow path of a specimen cartridge which includes the flow path and a detection vessel, the complex containing a test substance formed on magnetic particles and a labeled substance binding to the test substance; taking an image of the magnetic particles in the specimen cartridge; and measuring a label of the labeled substance contained in the complex in the detection vessel.

Abstract: The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications.

Abstract: Disclosed is a solid support suitable for improving the detection of how different species interact with each other. The solid support has multiple defined areas divided by low dividers, and optionally independent containers divided by high dividers, each container having at least two defined areas. The solid support is typically used in measurements where the total amount of liquid is temporarily reduced during measurement. The solid support extends the possibilities of the measurement system.

Abstract: The invention relates to methods, reagents and devices for detecting an analyte in a sample.

Abstract: Techniques for metal enhanced fluorescence include determining a calibration curve that relates concentration of a particular analyte to at least one of intensity or lifetime of fluorescent emissions at a plasmonic substrate in response to incident light, for a plurality of known concentrations of the particular analyte mixed with a reagent. The reagent comprises a detection molecule. A concentration of the particular analyte in a vicinity of a cell in a sample is determined directly from the calibration curve and measurements, in response to the incident light, of at least one of intensity or lifetime of fluorescent emissions at the plasmonic substrate in contact with the cell and reagent.