Patents Examined by Bennett Celsa
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Patent number: 6875594Abstract: The present invention provides a method of cleaving a recombinantly expressed protein from an intein and ligating the protein to a peptide containing an N-terminal cysteine having an unoxidized sulfhydryl side chain which comprises contacting the protein with the peptide in a reaction solution comprising a conjugated thiophenol, thereby forming a C-terminal thioester of the recombinant protein which spontaneously rearranges intramolecularly to form an amide bond linking the protein to the peptide.Type: GrantFiled: July 12, 2001Date of Patent: April 5, 2005Assignee: The Rockefeller UniversityInventors: Tom W. Muir, Philip A. Cole, Dolan Sondhi, Konstantine Severinov
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Patent number: 6872536Abstract: Radiopharmaceuticals comprising molecules that target to N-formyl-methionyl-leucy-phenylalanine (fMLF) receptor on leukocytes in order to target sites of inflammation for diagnostic imaging are described. The targeting molecules are attached to capping groups that make the entire molecule either antagonists or weak agonists of fMLF receptor and therefore do not elicit a chemotactic response resulting in neutropenia. The preferred targeting molecule is ReO-Gly-lys(Dimethylgly-t-Butylgly-cys-gly)glu-trp-phe-leu-nle-NHCOcyclopropyl (wherein the peptide sequence NLeu-Leu-Phe-Trp-Glu-Lys-Gly is SEQ. ID No. 1.) The invention also relates to the use of combinatorial chemistry to obtain preferred molecules that target sites of inflammation for diagnostic imaging.Type: GrantFiled: November 10, 1999Date of Patent: March 29, 2005Assignee: Bracco Imaging S.p.A.Inventors: David Roe, Christine Maria Ribic, Betty Chee-Ion Lawrence, Catherine Michelle Pollock, Alfred Pollack
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Patent number: 6872535Abstract: A three-dimensional (3D) array of solid-phase supports is adapted to provide parallel synthesis of a library of molecules with 3D diversity. Individual locations in the 3D array may be assigned to selected molecules in the library such that molecules may be synthesized at and retrieved from such locations. Also, the supports include aperture walls in stacked plates; the supports may be suspended within stacked plate apertures; the 3D array include discrete supports arranged in columns in one or more wells; the supports include tube inner walls or be suspended in tubes, the tubes being secured in stacked, two-dimensional (2D) frameworks; or the supports include beads contained in porous enclosures having non-porous side walls and being secured in stacked, 2D frameworks. A support transfer device enables transfer of solid-phase supports used in a 3D array.Type: GrantFiled: May 20, 1998Date of Patent: March 29, 2005Assignee: Aventis Pharmaceuticals Inc.Inventor: Stephen A. Baum
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Patent number: 6872825Abstract: The subject invention involves compounds having structure (I), wherein: (a) R1 is hydrogen or alkyl; and R2 is selected from hydrogen, alkyl, aryl, heterocyclyl, carboxy and its esters and amides; or R1 and R2 are attached and are together alkylene or heteroalkylene; (b) R4 is selected from aryl, heteroaryl, and ?,?-unsaturated conjugated aryl or heteroaryl; and (c) R5 is selected from hydrogen, alkyl, aryl, and heterocyclyl; and an optical isomer, diesteriomer, or enantiomer thereof; a salt, hydrate, ester, amide or imide thereof. The subject invention also includes libraries of such compounds, and processes for making the subject compounds and libraries.Type: GrantFiled: December 20, 2000Date of Patent: March 29, 2005Assignee: The Procter & Gamble CompanyInventors: Adam Golebiowski, Sean Rees Klopfenstein
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Patent number: 6872393Abstract: The present invention relates to the chemical modification of single chain polypeptides by means of covalent attachment of strands of poly(ethylene glycol) PEG and similar poly(alkylene oxides) to single chain polypeptide binding molecules that have the three dimensional folding and, thus, the binding ability and specificity, of the variable region of an antibody. Such preparations of modified single chain polypeptide binding molecules have reduced immugenicity and antigenicity as well as having a longer halflife in the bloodstream as compared to the parent polypeptide. These beneficial properties of the modified single chain polypeptide binding molecules make them very useful in a variety of therapeutic applications. The invention also relates to multivalent antigen-binding molecules capable of PEGylation. Compositions of, genetic constructions for, methods of use, and methods for producing PEGylated antigen-binding proteins are disclosed.Type: GrantFiled: February 26, 2001Date of Patent: March 29, 2005Assignee: Enzon, Inc.Inventors: Marc Whitlow, Robert G. L. Shorr, David R. Filpula, Lihsyng Standford Lee
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Patent number: 6872803Abstract: The present invention provides peptides represented by the following formula (A): wherein Q represents a physiologically active peptide moiety; X each represents the same or different ?-amino acid residue; M represents Gly or Cys; m represents an integer of from 5 to 8; and n represents an integer of from 0 to 3, or their pharmaceutically acceptable salts thereof. The peptides of the present invention have higher stability and/or higher activity than physiologically active linear peptides to which no cyclic peptide is bonded.Type: GrantFiled: July 8, 1999Date of Patent: March 29, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Kenji Shibata, Toshiyuki Suzawa, Motoo Yamasaki, Koji Yamada, Tatsuhiro Ogawa, Takeo Tanaka, Shiro Soga
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Patent number: 6864048Abstract: A library for determining the sequence of monomers in a polymer which is complementary to a receptor includes groups of pooled polymer products; wherein each pool is ordered such that a monomer sequence which binds to a receptor can be identified from the pool order in the library.Type: GrantFiled: April 28, 1993Date of Patent: March 8, 2005Assignee: Affymetrix, Inc.Inventors: Stephen P. A. Fodor, Lubert Stryer
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Patent number: 6864060Abstract: The present invention pertains to novel yeast cells which are useful for the expression of heterologous G protein coupled receptors. The yeast cells of the present invention can be used in screening assays which can be used to screen for modulators of G protein coupled receptors. Specifically, the invention provides novel yeast cells which express a heterologous G protein coupled receptor and mutant and/or chimeric G protein subunit molecules which serve to functionally integrate the heterologous into the pheromone signaling pathway of the yeast cell. The invention also provides for the expression of heterologous G protein coupled receptors which are functionally integrated into the yeast cell membrane using a yeast ? factor leader sequence. Drug discovery assays using the subject yeast cells are also provided.Type: GrantFiled: October 7, 1997Date of Patent: March 8, 2005Assignee: Cadus Technologies, Inc.Inventors: Dana M. Fowlkes, James R. Broach, John P. Manfredi, Jeremy I. Paul, Joshua Trueheart, Christine A. Klein, Andrew J. M. Murphy
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Patent number: 6861533Abstract: This invention provides methods for the solution-phase synthesis of arylbenzoxazoles. The methods involve condensation of aminophenols with benzaldehydes to form a Schiff base. The Schiff base is then induced to undergo oxidative cyclization in the presence of DDQ. The resulting arylbenzoxazoles can be separated from the reduced DDQ byproduct by treatment of reaction mixture with a strongly basic ion exchange resin.Type: GrantFiled: November 19, 2002Date of Patent: March 1, 2005Assignee: IRM LLCInventors: Junbiao Chang, Shifeng Pan
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Patent number: 6861523Abstract: The solid-phase synthesis of individual 1,3-disubstituted and 1,3,5-trisubstituted-1,3,5-triazine-2,4,6-triones and libraries thereof from a resin is described. Reaction of resin-bound amino acids with isocyanates yields resin-bound ureas, which further react with chlorocarbonyl isocyanate to selectively afford the resin-bound 1,3-disubstituted-1,3,5-triazine-2,4,6-triones. Selective alkylation at the N-5 position of the resin-bound 1,3-disubstituted-1,3,5-triazine-2,4,6-triones produces a trisubstituted triazinetrione. The products are cleaved from their solid support and obtained in good yield and purity.Type: GrantFiled: February 8, 2002Date of Patent: March 1, 2005Assignee: Torrey Pines Institute for Molecular StudiesInventors: Yongping Yu, John M. Ostresh, Richard A. Houghten
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Patent number: 6858719Abstract: The invention includes Rh(D) binding proteins, including antibodies, and DNA encoding such proteins. Methods of generating such proteins and DNAs are also included.Type: GrantFiled: May 4, 2001Date of Patent: February 22, 2005Assignee: The Trustees of the University of PennsylvaniaInventor: Donald L. Siegel
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Patent number: 6858423Abstract: Interactions between molecules which are components of self-assembled monolayers and other molecules can be amplified and transduced into an optical signal through the use of a mesogenic layer. The invention provides a device and methods for detecting analytes. The device comprises a substrate onto which a self-assembled monolayer is attached and a mesogenic layer which is anchored by the self-assembled monolayer. The mesogenic layer undergoes a change in conformation in response to the molecular interaction.Type: GrantFiled: August 10, 2000Date of Patent: February 22, 2005Assignee: The Regents of the University of CaliforniaInventors: Nicholas L. Abbott, Justin J. Skaife, Vinay K. Gupta, Timothy B. Dubrovsky, Rahul Shah
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Patent number: 6855691Abstract: Nitric oxide (NO) interacts with hemoglobin (Hb) at its metal centers, whereas S-nitrosothiols (RSNOs) can donate the NO group to ?93 cysteine residues, thereby shielding the NO functionality from heme inactivation. S-nitrosylation of Hb is under the allosteric control of oxygen and the oxidation state of heme. NO group release from S-nitrosohemoglobin (SNO-Hb) is further facilitated by intracellular low molecular weight thiols, forming RSNOs which can be exported from the erythrocyte to regulate blood pressure. Hence, a dynamic cycle is established in which S-nitrosylation of Hb is initiated in the lung following oxygenation of red blood cells and is completed by SNO-Hb metabolism during arterial-venous transit. SNO-Hb can be formed by reaction of Hb with S-nitrosothiol. This procedure avoids oxidation of the heme.Type: GrantFiled: March 15, 1996Date of Patent: February 15, 2005Assignee: Duke UniversityInventor: Jonathan S. Stamler
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Patent number: 6852690Abstract: A composition and method for improved parenteral administration of nutrients is provided. The method comprises co-administration of nutrients, especially carbohydrates, and an insulinotropic peptide, or its derivatives, analogs, and fragments. The method and composition provide high carbohydrate nutrition while avoiding hyper- and hypoglycemia and their attendant deleterious effects.Type: GrantFiled: August 22, 1996Date of Patent: February 8, 2005Assignee: Amylin Pharmaceuticals, Inc.Inventors: Michael A. Nauck, Fred A. Wagner
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Patent number: 6852285Abstract: Interactions between molecules which are components of self-assembled monolayers and other molecules can be amplified and transduced into an optical signal through the use of a mesogenic layer. The invention provides a device and methods for detecting analytes. The device comprises a substrate onto which a self-assembled monolayer is attached and a mesogenic layer which is anchored by the self-assembled monolayer. The mesogenic layer undergoes a change in conformation in response to the molecular interaction.Type: GrantFiled: July 3, 2001Date of Patent: February 8, 2005Assignee: The Regents of the University of CaliforniaInventors: Nicholas L. Abbott, Justin J. Skaife, Vinay K. Gupta, Timothy B. Dubrovsky, Rahul Shah
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Patent number: 6844161Abstract: Novel proteins and protein libraries are disclosed. The proteins possess one or more functional protein modules from different parent protein molecules. The proteins and protein libraries are exemplified by the preparation of cross-over chemokines that contain various combinations of peptide segments derived from RANTES, SDS-1 and vMIP-I and to vMIP-II. The proteins and libraries are extremely pure and can be provided in non-limiting high yields suitable for diagnostic and high-throughput screening assays.Type: GrantFiled: August 31, 1998Date of Patent: January 18, 2005Assignee: Gryphon Therapeutics, Inc.Inventors: Michael A. Siani, Jill Wilken, Reyna Simon, Stephen B. H. Kent
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Patent number: 6843993Abstract: A non-food anti-microbial-adhesion and aggregation composition comprising a suitable carrier and an effective amount of an adhesion inhibitory fraction isolated from juice from berries of the Vaccinium plant genus. In an embodiment the anti-aggregation and adhesion fraction is isolated from cranberry juice. It is characterized as being polymeric and having a molecular weight ?14,000; an elemental analysis of carbon 43-51%, hydrogen 4-5%, no nitrogen, no sulfur and no chlorine; a nuclear magnetic resonance (NMR) line spectrum as set forth in FIGS. 2A and 2B; and an ultraviolet spectrum with an absorption peak at 280 nm in neutral or acidic pH solution which is absent in alkali solutions. This fraction exhibits adhesion inhibitory activity against P fimbriated bacteria, oral bacteria and Helicobacter pylori.Type: GrantFiled: July 27, 2001Date of Patent: January 18, 2005Assignee: Ramot at Tel-Aviv University, Ltd.Inventors: Itzhak Ofek, Ervin Weiss, Yoel Kashman, Janina Goldhar, Nathan Sharon
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Patent number: 6841657Abstract: Inhibitors of HIV membrane fusion and a method of identifying drugs or agents which inhibit binding of the N-helix coiled-coil and the C helix of HIV gp41 envelope protein.Type: GrantFiled: December 21, 2000Date of Patent: January 11, 2005Assignee: Whitehead Institute for Biomedical ResearchInventors: Debra M. Eckert, David C. Chan, Vladimir Malashkevich, Peter A. Carr, Peter S. Kim
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Patent number: 6841530Abstract: Disclosed are semi-synthetic methods for the preparation of Didemnin Analogs. The compounds of this type are illustrated in Formula (I).Type: GrantFiled: September 10, 2001Date of Patent: January 11, 2005Assignee: The Board of Trustees of the University of IllinoisInventors: Kenneth L. Rinehart, Alexandra J. Katauskas
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Patent number: 6838238Abstract: This invention provides a method for identifying one or more complexes from a library of complexes, wherein said complex or complexes are selected for their ability to perform a preselected or desired function on a target molecule or by having a pre-selected structure, each complex being designated a morphatide, said method comprising: (a) preparing a library of morphatides, comprised of: (i) a scaffolding component selected from the group consisting of nucleic acid, nucleic acid like molecule or nucleic acid analog having one or more regions of randomized sequence; (ii) one or more linker components; and (iii) one or more agent molecules or type of agent molecules, linked to the scaffolding component by one or more type of linker components; and (b)screening the library of morphatides prepared in step (a) by contacting, binding, or associating the morphatides with one or more suitable target molecules upon which a morphatide performs a preselected or desired function or to which a morphatide binds or associaType: GrantFiled: April 14, 1997Date of Patent: January 4, 2005Assignee: Invitrogen CorporationInventor: Jay M. Short