Patents Examined by Bin Shen
  • Patent number: 10416144
    Abstract: Methods for detecting biomarkers of inflammation, infection, and/or bacterial activity in dairy production, which indicate issues with the milk itself or issues related to the health of the cow. The methods generally comprise contacting a milk sample with a nanoplatform assembly to create an assay solution, and detecting spectral changes in the assay solution that are triggered by enzymatic activity (when present) in the sample. The nanoplatform assembly comprises a first particle, a second particle, and a linkage therebetween, wherein the linkage comprises a protease consensus sequence (the sequence of amino acids cleaved by the protease), or an ester linkage (cleaved by a protease or lipase). A plurality of second particles can also be linked to the first particle. Test strips are also described, which undergo a visual color change in the presence of the target enzyme in the milk sample.
    Type: Grant
    Filed: July 27, 2015
    Date of Patent: September 17, 2019
    Assignee: Kansas State University Research Foundation
    Inventors: Deryl L. Troyer, Stefan H. Bossmann, Aruni P. Malalasekera, Thilani N. Samarakoon, Hongwang Wang, Madumali Kalubowilage
  • Patent number: 10415077
    Abstract: Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.
    Type: Grant
    Filed: September 11, 2008
    Date of Patent: September 17, 2019
    Assignee: DENKA SEIKEN CO., LTD.
    Inventor: Yuhko Hirao
  • Patent number: 10393731
    Abstract: The present invention relates to cell-based methods for determining the biological activity of defibrotide. In particular, the invention provides a method for assessing the potency of defibrotide by assessing the viability of mammalian cells in the presence of at least one cytotoxic agent and one or more concentrations of defibrotide. Such methods are particularly useful for standardizing pharmaceutical compositions comprising defibrotide.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: August 27, 2019
    Assignee: GENTIUM S.R.L.
    Inventors: Terenzio Ignoni, Vijay Kumar, Claudio Verga
  • Patent number: 10392650
    Abstract: Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: August 27, 2019
    Assignee: Theranos IP Company, LLC
    Inventors: Douglas Matje, Ian Gibbons, Paul Patel, Elizabeth A. Holmes
  • Patent number: 10385306
    Abstract: A device for single-cell analysis according to an embodiment of the present invention comprises: a substrate; a gap between the substrate and porous membrane which is a space for culture medium; and a porous membrane formed on having a pore capable of isolating a second cell into single cell units. A method for single-cell analysis according to an embodiment of the present invention comprises: Culturing a first cell in a culture medium on a bottom side of porous membrane; Applying a sample including a second cell on a porous membrane in a culture medium; Isolating the second cell into single cell units in a pore existing in the porous membrane with a external force such as agitation and gravitational force; Generating an interaction situation between the first cells and the single cell-level second cell; Analyzing a cellular phenomena of the first cell or the second cell.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: August 20, 2019
    Inventors: Yoon Kyoung Cho, Cedric Bathany, Jun Young Kim, Devrim Gozuacik
  • Patent number: 10383978
    Abstract: A dry animal-derived collagen fiber tissue material and preparation method and bioprosthesis thereof are disclosed. The preparation method includes: 1) rinsing of an animal-derived collagen fiber tissue material that has been treated with a crosslinking agent; 2) immersion of the rinsed tissue material in a non-aqueous alcoholic solution for dehydration; 3) successive immersion of the tissue material that has been dehydrated with the non-aqueous alcoholic solution in saccharide solutions of different gradients of concentrations for gradient dehydration; 4) taking out and drying of the gradient dehydrated tissue material; and 5) hermetic packaging of the dried tissue material and sterilization. The preparation method is simple and allows the use of easily-available low-cost materials, resulting in lower costs. In addition, it can reduce the toxicity caused by a residue of the aldehyde crosslinking agent.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: August 20, 2019
    Inventors: Jiaoming Dong, Yemeng Chen, Guanbiao Xu, Xiulan Cheng, Guoming Chen, Yu Li, Qiyi Luo
  • Patent number: 10376601
    Abstract: An in-vivo intravascular blood replacing liquid of the present invention is injected into a blood vessel to replace blood at an intravascular portion to be inspected therewith in making an in-vivo intravascular inspection. The blood replacing liquid comprises an aqueous medium unharmful for a living body and a gelling property imparting substance, unharmful for the living body, which is added to the aqueous medium to impart a gelling property to the blood replacing liquid. The blood replacing liquid has a viscosity of not more than 3 mPa·s when the blood replacing liquid is injected into the blood vessel.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: August 13, 2019
    Inventors: Koji Nakamura, Isao Mori, Masanori Tokida
  • Patent number: 10370685
    Abstract: Processes are disclosed for economically and effectively removing co-produced oxygenated organic compound from an anaerobic, aqueous fermentation broth used for the bioconversion of syngas to product oxygenated organic compound. The processes involve subjecting a portion of the aqueous fermentation broth after recovery of the product oxygenated organic compound to anaerobic organic bioconversion, and recycling the broth for use in the bioconversion of syngas.
    Type: Grant
    Filed: July 9, 2014
    Date of Patent: August 6, 2019
    Assignee: Synata Bio, Inc.
    Inventors: Chad Hatten, Robert Hickey
  • Patent number: 10364415
    Abstract: To provide an effective 1,4-dioxane-degrading bacteria culture method. Provided is a 1,4-dioxane-degrading bacteria culture method in which 1,4-dioxane-degrading bacteria are propagated using a medium containing diethylene glycol.
    Type: Grant
    Filed: October 7, 2015
    Date of Patent: July 30, 2019
    Inventors: Norifumi Yamamoto, Yuji Saito, Michihiko Ike, Masashi Kuroda, Kazunari Sei, Daisuke Inoue
  • Patent number: 10357532
    Abstract: A protein transduction method for efficiently delivery of exogenous proteins into mammalian cells is invented, which has the capability of targeting different cellular compartments and protection from degradation of the delivered proteins from cellular proteases. A composition for treat proteins has cation reagents, lipids and enhancers in a carrier. The method can be used in a number of ways including: production of large quantities of properly folded, post-translationally modified proteins using mammalian cell machinery, a in-cell fluorescence spectroscopy and imaging using small molecule fluorophores and a in-cell NMR spectroscopy using living mammalian cells. The method permits cell biology at atomic resolution that is physiologically and pathological relevant and permits protein therapy to treat human diseases. The method can also be used to deliver exogenous protein inside mammalian cells, wherein the exogenous proteins follow a similar secretion pathway as that of the endogenous protein.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: July 23, 2019
    Assignee: Wayne State University
    Inventors: Jianjun Wang, Qianqian Li
  • Patent number: 10357780
    Abstract: Disclosed herein is an improved method for magnetic capture of target molecules (e.g., microbes) in a fluid. Kits and solid substrates for carrying the method described herein are also provided. In some embodiments, the methods, kits, and solid substrates described herein are optimized for separation and/or detection of microbes and microbe-associated molecular pattern (MAMP) (including, e.g., but not limited to, a cell component of microbes, lipopolysaccharides (LPS), and/or endotoxin).
    Type: Grant
    Filed: October 27, 2015
    Date of Patent: July 23, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Joo Hun Kang, Donald E. Ingber, Michael Super
  • Patent number: 10357548
    Abstract: The present application provides for compositions comprising ?-galactosidase A in combination with an active site-specific chaperone for the ?-galactosidase A, and methods for treating Fabry disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an ?-galactosidase A enzyme formulation.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: July 23, 2019
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Richie Khanna, Kenneth Valenzano, Susan Elizabeth Fowles
  • Patent number: 10359345
    Abstract: Provided are a method for preparing a liquid-state dripping or coating pathological quality control product, and uses thereof. The method comprises: selecting and determining a control with a control value, and processing the control; adding an ethanol solution to the processed control for preserving for standby use, with the amount of the ethanol solution added depending on the amount of a precipitate; and performing setting of a positive or negative control by means of dripping or smearing. The pathological quality control product is a suspension or homogenate of micro tissue sections, cell/cultured cell sections, or cultured cells. Another aspect of the present invention provides a use of the liquid-state dripping or coating pathological quality control product as a positive or negative control in immunohistochemistry, in situ hybridization, special staining and other tissue staining detection, or as a standard quality control product for pathological internal quality control and external quality control.
    Type: Grant
    Filed: April 23, 2015
    Date of Patent: July 23, 2019
    Inventor: Wei Ding
  • Patent number: 10344313
    Abstract: The invention relates to a method for detecting Serpula lacrymans contamination in an internal environment, taking into account the absence and presence of VOCs produced by the metabolism of Serpula lacrymans, especially by means of the calculation of a contamination index.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: July 9, 2019
    Inventors: Stéphane Moularat, Enric Robine
  • Patent number: 10344254
    Abstract: Reactor plant and process for culturing phototropic microorganisms with a reactor vessel and a photosynthetic module, which are connected with one another in a circulation circuit by a pump and can be controlled via a control unit, where in the circulation circuit or in the reactor vessel, a homogenizer is provided, where a light-intensity control of the photosynthetic module is provided, and where a bypass of the circulation circuit or of the reactor vessel with a filter device which is arranged externally of the reactor vessel for separating off extracellular products during the fermentation is provided.
    Type: Grant
    Filed: March 6, 2007
    Date of Patent: July 9, 2019
    Assignee: Sartorius AG
    Inventors: Wolfgang Kahlert, Bernd-Ulrich Wilhelm, Wolf-Dietrich Linke
  • Patent number: 10344253
    Abstract: Devices for the propagation or storage of microorganisms are provided including a first layer that has a first portion of a surface of the first layer, to which a first water-swellable gelling agent comprising a first clay is affixed. The devices further include a second layer that is separable from the first layer and has a first portion of a surface of the second layer, to which a second water-swellable gelling agent is affixed. Methods for detecting and enumerating at least one microorganism in a sample are provided. The methods include providing a device, separating the first layer from the second layer, adding an aliquot of a sample containing at least one microorganism onto the first or second water-swellable gelling agent to form an inoculated device, laminating the first layer back to the second layer, and incubating the inoculated device. Kits and methods of making the devices are also provided.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: July 9, 2019
    Assignee: 3M Innovative Properties Company
    Inventors: Evan D. Brutinel, Alexi J. Young, Adam J. Stanenas
  • Patent number: 10329631
    Abstract: An object of the present invention is to provide a constitutive 1,4-dioxane-degrading bacteria offering excellent maximum relative rates of degradation of 1,4-dioxane. As a means for achieving the object, the present invention provides a constitutive 1,4-dioxane-degrading bacteria being strain N23 deposited as Accession No. NITE BP-02032.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: June 25, 2019
    Inventors: Norifumi Yamamoto, Yuji Saito, Hironori Taki
  • Patent number: 10323265
    Abstract: An improved method and related apparatus for detecting bacteria viability and drug effects using metabolic monitoring. A fluorescent material which is quenched by oxygen is co-localized with the target bacteria, and fluorescence signal is detected at the co-localized places. In some embodiments, the fluorescent material is a fluorescent nanoparticle mixed with the target bacteria in the sample, and co-localization is enhanced using centrifugation, electrophoresis, microflow path modified with antibodies, magnetic force, etc. In some other embodiments, the fluorescent material is a fluorescent film or 3-D matrix immobilized in the bacterial culture chamber, and bacteria in the sample is gathered into localized regions of the bacteria culture chamber where the fluorescent film or 3-D matrix is present by ways of centrifugation, electrophoresis or microflow path. Plasmonic nanoparticles with a metal core and plasmonic film with a metal film may be used as the fluorescent nanoparticles and fluorescent film.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: June 18, 2019
    Inventor: Noriaki Yamamoto
  • Patent number: 10316052
    Abstract: A fidaxomicin purification method, comprising: fermenting Actinoplanes sp. HS-16-20 to generate fermented liquid; conducting solid/liquid separation on the fermented liquid, soaking mycelium in an organic solvent, and filtering to obtain a solution containing fidaxomicin; treating the solution with nanofiltration concentrate, and separating to obtain fidaxomicin crude product; conducting preparative column chromatography on the fidaxomicin crude product, eluting with an acid aqueous solution containing an organic solvent, and separating to obtain the refined fidaxomicin product.
    Type: Grant
    Filed: July 7, 2015
    Date of Patent: June 11, 2019
    Inventors: Huijun Ren, Daochao Li, Xuexiao Ying, Feng Chen, Linghui Zheng, Lingping Wang, Hua Bai
  • Patent number: 10300034
    Abstract: The present invention provides dietary formulations comprising polyunsaturated fatty acids and vitamin E. The present invention further provides methods of treating various conditions, generally involving administering to an individual in need thereof an effective amount of a subject dietary formulation.
    Type: Grant
    Filed: February 19, 2008
    Date of Patent: May 28, 2019
    Inventor: Claudia R. Morris