Patents Examined by Bin Shen
  • Patent number: 11363820
    Abstract: The present invention provides a screening method for selecting plant symbiotic microorganisms enabling plant growth under abiotic stress and/or biotic stress, a microbial mixture of microorganisms which are selected from nature by the method and enables a plant to grow under salinity stress and/or hyperosmotic stress in symbiosis with the plant. Specifically, the present invention provides a screening method for selecting plant symbiotic microorganisms, comprising a first screening step of allowing at least one plant to grow for a predetermined period of time while being partially soaked in a solution containing a microbial mixture under abiotic stress and/or biotic stress, and collecting microorganisms adhering to grown plant to thereby select out the microorganisms as plant symbiotic microorganisms enabling plant growth under the stress.
    Type: Grant
    Filed: December 8, 2017
    Date of Patent: June 21, 2022
    Inventor: Seigo Ono
  • Patent number: 11365388
    Abstract: Provided is a method of producing a novel microorganism having a minimal genome. In particular, provided are a method of producing a novel microorganism having a smaller genome size than a wild-type, the method including the step of culturing the microorganism in a medium containing LB broth with stepwise decreasing concentrations of the LB broth; and a novel microorganism having a smaller genome size than a wild-type, produced by the above method. Despite the small genome size, the novel microorganism of the present disclosure may have a high growth rate, pyruvate production capacity, and protein translation efficiency thereby being usefully applied to industry such as production of recombinant proteins or pyruvate.
    Type: Grant
    Filed: January 24, 2020
    Date of Patent: June 21, 2022
    Inventors: Sun-Chang Kim, Byung-Kwan Cho, Donghui Choe, Bong Hyun Sung, Jun Hyoung Lee
  • Patent number: 11345886
    Abstract: The presently disclosed subject matter relates, in general, to the identification, isolation, and use of a population of stem cells isolated from umbilical cord blood, peripheral blood and/or other sources and that are referred to herein as Small Mobile Stem cells (short: SMS). More particularly, the presently disclosed subject matter relates to isolating said SMS stem cells and employing the same, optionally after in vitro manipulation, to treat tissue and/or organ damage in a subject in need thereof.
    Type: Grant
    Filed: July 18, 2018
    Date of Patent: May 31, 2022
    Assignee: SMSBIOTECH, INC.
    Inventor: Abdulkader Rahmo
  • Patent number: 11332711
    Abstract: Described herein are compositions and methods to cryopreserve sporozoites. In some aspects, the method can include the step of placing harvested salivary glands containing sporozoites into an insect-based medium.
    Type: Grant
    Filed: April 5, 2019
    Date of Patent: May 17, 2022
    Inventors: John H. Adams, Alison Elizabeth Roth
  • Patent number: 11332712
    Abstract: A cell culture medium comprising adenosine triphosphate; a carrier protein; cholesterol, linoleic acid, and lipoic acid; glutathione; at least one nucleotide salvage pathway precursor base; phosphoethanolamine; selenium; transferrin; triiodothyronine; all-trans-retinoic acid (ATRA) and vitamin C; zinc, magnesium, and copper; an agent that increases intracellular cAMP; epidermal growth factor (EGF); hydrocortisone; insulin; and charcoal stripped fetal bovine serum, wherein said cell culture medium is substantially free, if not entirely free, of vitamin D, androgenic hormones, androgenic ligands, estrogenic hormones, estrogenic ligands, and/or androgenic receptors.
    Type: Grant
    Filed: October 10, 2019
    Date of Patent: May 17, 2022
    Inventor: Tan Ince
  • Patent number: 11327030
    Abstract: The present invention addresses the problem of providing a method for observing skin tissue in which x-ray microCT is used. Adnexae such as sweat glands, sebaceous glands, and hair follicles can be identified in skin tissue by using acetone as a pretreatment solution and staining using an iodine-containing solution as a staining solution.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: May 10, 2022
    Assignee: Shiseido Company, Ltd.
    Inventor: Tomonobu Ezure
  • Patent number: 11311008
    Abstract: A cryopreservation composition generally includes a cryoprotective agent and a laser absorber that includes a metal. Rewarming a cryopreserved specimen that includes the cryopreservation composition includes subjecting the cryopreserved specimen to a laser pulse effective to heat the laser absorber sufficiently to thaw the cryopreserved biospecimen.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: April 26, 2022
    Inventors: John Bischof, Kanav Khosla, Zhenpeng Qin, Mary Margaret Hagedorn, Cari Suzanne Dutcher
  • Patent number: 11307146
    Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: April 19, 2022
    Assignees: University of Houston System, Instituto Tecnológico y de Estudios Superiores de Monterrey
    Inventors: Richard C. Willson, Jinsu Kim, Binh V. Vu, Olga Patricia Vázquez Villegas, Federico Augusto Ruiz Ruiz, Marco Antonio Rito Palomares
  • Patent number: 11307201
    Abstract: An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample, and washing any such bound target. The bound target, if not immobilized, may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action of the enzyme upon the substrate frees electrons, creating a potential across an anode and cathode which may be separated by a membrane.
    Type: Grant
    Filed: September 17, 2008
    Date of Patent: April 19, 2022
    Assignee: RED IVORY LLC
    Inventors: Marc Robert Labgold, George G Jokhadze
  • Patent number: 11293039
    Abstract: The invention relates to a method for producing salts of butyric acid, lactic acid, propionic acid, valeric acid, acetic acid, glycolic acid, sorbic acid, fumaric acid, formic acid, malic acid, tartaric acid, citric acid, derivatives of said organic acids and mixtures thereof, comprising at least the successive steps of: •i) culturing a microorganism in an appropriate culture medium comprising a carbon source and a nitrogen source, during a sufficient time to obtain a fermentation broth comprising the organic acid(s); •ii) pre-treating said fermentation broth comprising at least clarification of the fermentation broth and separation of organic acids by evaporation of the clarified fermentation broth and then condensation of the volatile fraction containing organic acids (CVAF); •v) adding inorganic bases to said CVAF •vii) eliminating the remaining water of the CVAF, and recovering the organic acid salts.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: April 5, 2022
    Inventors: Antoine Sevenier, Cédric Colomb, Benjamin Martin
  • Patent number: 11280711
    Abstract: A method including placing a biological sample taken from a body into a chamber; adding a composition including an acetal solvent to the chamber; and fixating the biological sample. A method including placing a tissue from a body into a chamber; and contacting the tissue with a composition including an acetal solvent as a fixating process.
    Type: Grant
    Filed: October 8, 2018
    Date of Patent: March 22, 2022
    Assignee: Sakura Finetek U.S.A., Inc.
    Inventor: Amit D. Shah
  • Patent number: 11280707
    Abstract: A process for making and using a tissue preservation gel. The gel is comprised of water, reticulated acrylic acid, water soluble short-chain paraben, isopropyl alcohol and either triethanolamine or propylene glycol. To use the gel, a tissue to be preserved is first bathed in hypertonic sodium chloride, then soaked in a wash in multiple increasing concentrations of isopropyl alcohol and then preserved indefinitely in the tissue preservation gel.
    Type: Grant
    Filed: May 16, 2021
    Date of Patent: March 22, 2022
    Inventor: Clorinda V. Contreras Villasmil
  • Patent number: 11268130
    Abstract: Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed.
    Type: Grant
    Filed: August 2, 2019
    Date of Patent: March 8, 2022
    Assignee: Labrador Diagnostics LLC
    Inventors: Douglas Matje, Ian Gibbons, Paul Patel, Elizabeth A. Holmes
  • Patent number: 11266085
    Abstract: The method of growing a biopolymer material employs incubation of a growth media comprised of nutritive substrate and a fungus in containers that are placed in a closed incubation chamber with air flows passed over each container while the chamber is maintained with a predetermined environment of humidity, temperature, carbon dioxide and oxygen. The air flows may be directed parallel or perpendicularly to the surfaces of the growth media.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: March 8, 2022
    Inventors: Jessie Hannah Kaplan-Bie, Lucy Elaine Greetham, Ian Thomas Bonesteel, Gavin Reim McIntyre
  • Patent number: 11255786
    Abstract: The stiffness and topology of ultra-small circular DNAs and DNA/peptide hybrids are exploited to create a transducer of enzyme activity with low error rates. The modularity and flexibility of the concept are illustrated by demonstrating various transducers that respond to either specific restriction endonucleases or to specific proteases. In all cases the output is a DNA oligo signal that, as we show, can readily be converted directly to an optical readout, or can serve as input for further processing, for example, using DNA logic or amplification. By exploiting the DNA hairpin (or stem-loop) structure and the phenomenon of strand displacement, an enzyme signal is converted into a DNA signal, in the manner of a transducer. This is valuable because a DNA signal can be readily amplified, combined, and processed as information.
    Type: Grant
    Filed: June 16, 2021
    Date of Patent: February 22, 2022
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Mario Ancona, Hieu Bui
  • Patent number: 11241499
    Abstract: Drug formulations and methods for removing, reducing, or preventing the formation of fatty acid particles in drug formulations are provided.
    Type: Grant
    Filed: March 3, 2020
    Date of Patent: February 8, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Dorothy Kim, Michael Marlow
  • Patent number: 11237167
    Abstract: Provided herein are methods, systems, and compositions for Lp-PLA2 detection assays that employ amounts of detergent to liberate all or nearly all of the Lp-PLA2 molecules from associated lipoprotein particles. In this regard, the true Lp-PLA2 concentration can be detected in a sample, which correlates better with known Lp-PLA2 activity assays.
    Type: Grant
    Filed: January 18, 2018
    Date of Patent: February 1, 2022
    Assignee: Cleveland Heartlab, Inc.
    Inventors: Celalettin Topbas, Cory Bystrom
  • Patent number: 11220704
    Abstract: Provided is a method of avoiding the influence of a coexisting substance in the measurement of HbA1c % for a whole blood sample by an enzymatic method. Specifically, provided is a method of measuring a ratio of a hemoglobin A1c concentration to a hemoglobin concentration in a sample by an enzymatic method, the method including: a first step of optically measuring the hemoglobin concentration; and a second step of optically measuring the hemoglobin A1c concentration, wherein, when HbA1c % is calculated by dividing the hemoglobin A1c concentration measured in the second step by the hemoglobin concentration measured in the first step, the hemoglobin concentration serving as a denominator, which is measured at a first wavelength, is corrected using a result measured at a second wavelength.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: January 11, 2022
    Inventors: Satoshi Machida, Tomohisa Nishio, Kazuo Nakanishi
  • Patent number: 11220668
    Abstract: The present invention relates to the use of sulfocysteine and derivatives thereof as cell culture additives to reduce the trisulfide levels in proteins produced in cell culture.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: January 11, 2022
    Assignee: Merck Patent GmbH
    Inventor: Aline Zimmer
  • Patent number: 11213607
    Abstract: The invention provides a cell binding composition comprising a shear thinning gel wherein the shear thinning gel having attached to it one or more cell selective binding agents, or the shear thinning gel having dispersed therein a plurality of gel beads, the gel beads having attached to them one or more cell selective binding agents. Methods of enriching cells using the compositions and using the cells to treat injury or disease are also provided.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: January 4, 2022
    Inventors: Liam Grover, Paula Mendes, Richard Williams