Patents Examined by Brian J. Sines
  • Patent number: 10413900
    Abstract: The present disclosure provides microfluidic devices, systems and methods for sample preparation and/or analysis. A microfluidic device can include a first channel having a sequence of (n) chambers each having a first volume (v). The first channel can include one or more valves at opposing ends of the first channel that fluidically isolate the first channel. The microfluidic device can further include a second channel in fluid communication with the first channel. The second channel can include at least one second chamber having a total second volume that is at least equal to the total volume of the first channel (n*v). The second channel can include one or more valves at opposing ends of the second channel that fluidically isolate the second channel from the first channel.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: September 17, 2019
    Assignee: GENAPSYS, INC.
    Inventors: Seth Stern, Hesaam Esfandyarpour, David Eberhart
  • Patent number: 10406519
    Abstract: A flow cell including inlet and outlet ports in fluid communication with each other through a flow channel that extends therebetween. The flow channel includes a diffuser region and a field region that is located downstream from the diffuser region. The field region of the flow channel directs fluid along reaction sites where desired reactions occur. The fluid flows through the diffuser region in a first flow direction and through the field region in a second flow direction. The first and second flow directions being substantially perpendicular.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: September 10, 2019
    Assignee: ILLUMINA, INC.
    Inventors: Helmy A. Eltoukhy, Tarun Khurana, Behnam Javanmardi, Poorya Sabounchi, Majid Aghababazadeh
  • Patent number: 10408824
    Abstract: Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.
    Type: Grant
    Filed: November 24, 2015
    Date of Patent: September 10, 2019
    Assignee: OPKO Diagnostics, LLC
    Inventors: Vincent Linder, David Steinmiller, Samuel K. Sia
  • Patent number: 10401351
    Abstract: A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.
    Type: Grant
    Filed: March 25, 2019
    Date of Patent: September 3, 2019
    Assignees: Sysmex Corporation, Sysmex America, Inc.
    Inventors: Takaaki Nagai, Noriyuki Narisada, Daigo Fukuma, Masanori Imazu
  • Patent number: 10401350
    Abstract: A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.
    Type: Grant
    Filed: December 10, 2018
    Date of Patent: September 3, 2019
    Assignees: Sysmex Corporation, Sysmex America, Inc.
    Inventors: Takaaki Nagai, Noriyuki Narisada, Daigo Fukuma, Masanori Imazu
  • Patent number: 10393690
    Abstract: A flexible chemiresistor (CR) sensor for sensing a molecule of interest in a fluid (liquid or gas) is provided. The flexible CR sensor comprises a flexible chemiresistor (CR) module. The flexible CR module comprises a flexible substrate such polyethylene terephthalate (PET), polyethylene naphthalate (PEN) or polyimide (PI), and a thin film nanoparticle assembly assembled on the flexible substrate. The thin film nanoparticle assembly comprises metal or metal alloy core, ligand-capped nanoparticles and molecular linkers connecting the nanoparticles. The flexible CR sensor and an intelligent pattern recognition engine can be incorporated in a handheld device that can detect a molecule of interest in a fluid (e.g., a liquid or gas) accurately, rapidly, and without false positives. Any sensing array nanomaterial, pattern recognition, and compact/or electronic hardware can be integrated to achieve a desired detection limit and response speed.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: August 27, 2019
    Assignee: The Research Foundation of State University of New York
    Inventors: Chuan-Jian Zhong, Jin Luo, Lingyan Wang
  • Patent number: 10383915
    Abstract: Methods and systems for releasing growth factors are disclosed. In certain embodiments, a blood sample is exposed to a sequence of one or more electric pulses to trigger release of a growth factor in the sample. In certain embodiments, the growth factor release is not accompanied by clotting within the blood sample.
    Type: Grant
    Filed: August 30, 2016
    Date of Patent: August 20, 2019
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Vasile Bogdan Neculaes, Andrew Soliz Torres, Antonio Caiafa, Brian Dun-Lan Lee, Allen Lawrence Garner
  • Patent number: 10385893
    Abstract: An active microfluidic droplet generation device includes a droplet generation junction joining at least one continuous phase channel for carrying a ferrofluid, and a dispersed phase channel for carrying a dispersed phase (e.g., aqueous) flow. A miniature electropermanent magnet (EPM) upstream from the junction generates a magnetic field to modulate a flow rate of a ferrofluid in the continuous phase channel so that dispersed phase droplets are generated with volumes actively controlled on-demand and under continuous flow.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: August 20, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Jose I. Padovani Blanco, Ali Mohamed Ibrahim, Yasser Hussein Anis, Stefanie S. Jeffrey, Roger T. Howe
  • Patent number: 10386327
    Abstract: A bioFET cell for measuring a time dependent characteristic of an analyte bearing fluid includes a source, a drain, a semiconductive single wall carbon nanotube network layer extending between the source and drain electrodes and electrically coupled there between, a gate insulatively spaced from and disposed over and extending between the source and drain electrodes, a layer of at least one selected antibody disposed on and linked to the polymer layer to functionalize the semiconductive single wall carbon nanotube network layer to a selected target biomarker corresponding to the at least one selected antibody so that electron transport into the semiconductive single wall carbon nanotube network layer is facilitated, where the source, drain and gate electrodes with the carbon nanotube network layer form a defined channel through which the analyte bearing fluid may flow, and a high impedance source follower amplifier coupled to the source electrode.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: August 20, 2019
    Assignee: Sensor Kinesis Corporation
    Inventor: Josh Shachar
  • Patent number: 10379083
    Abstract: This invention relates to identification of organic or nonorganic molecules dissolved in liquid solutions based on their internal dipole moment. These molecules include and are not limited to viruses, microbes, bacteria, and in general pathogens. The liquid solution provides a specific dielectric constant, which is directly related to the internal dipole moment of the dissolved pathogen. An electronic device namely PtSi-Porous Si schottky junction is proposed as the pathogen detector. This device, which is made of PtSi alloy covering the pores of an n-type Silicon substrate, is a sensitive indicator of the dielectric constant of the material filling its pores. In particular, such a device has a unique reverse biased current-voltage (IV) relation that is sensitive to changes in electric fields around its surface, which change its breakdown voltage.
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: August 13, 2019
    Inventor: Farshid Raissi
  • Patent number: 10379072
    Abstract: According to one aspect, a monitoring device for detecting when an object may be subjected to a condition includes a processor, a first sensor, a second sensor, and a configuration circuit. A first sensor is polymer monolayer adapted to detect if the object is subjected to a magnitude of a first condition. A second sensor is a polymer bilayer adapted to detect if the object is subjected to a magnitude of a second condition. The resistance across the first sensor and second sensor are compared to determine whether an ambient/environmental condition has been detected. Indication of detection of an ambient/environmental condition, magnitude of the condition, and the time may be stored.
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: August 13, 2019
    Assignee: Cryovac, LLC
    Inventors: James W. Blease, Theodore F. Cyman, Jr., Alan R. Murzynowski, Kevin J. Hook
  • Patent number: 10371762
    Abstract: The inventive concepts presented herein relate to methods of identifying molecules identification of molecules using apparatuses including: electromagnetic write-head(s); magneto-resistive read sensor(s), and processor(s). An exemplary method includes magnetically exciting a molecule to be identified using an alternating magnetic field generated by an electromagnetic write-head, measuring a resonant response of the molecule to be identified using a magneto-resistive read sensor; and comparing, using a processor, the resonant response of the molecule to be identified with a table of known resonant responses to identify a chemical composition of the molecule to be identified. The molecule to be identified may optionally be disposed on a biosample substrate which comprises, or is coupled to, a plurality of servo-alignment marks; and the plurality of servo-alignment marks are configured to facilitate alignment of the electromagnetic write-head with the biosample tracks of the biosample substrate.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: August 6, 2019
    Assignee: International Business Machines Corporation
    Inventors: Allen K. Bates, Anna W. Topol, Daniel J. Winarski
  • Patent number: 10369200
    Abstract: Methods and systems for releasing growth factors are disclosed. In certain embodiments, a blood sample is exposed to a sequence of one or more electric pulses to trigger release of a growth factor in the sample. In certain embodiments, the growth factor release is not accompanied by clotting within the blood sample.
    Type: Grant
    Filed: August 30, 2016
    Date of Patent: August 6, 2019
    Assignee: GENERAL ELECTRIC COMPANY
    Inventors: Vasile Bogdan Neculaes, Andrew Soliz Torres, Antonio Caiafa, Brian Dun-Lan Lee, Allen Lawrence Garner
  • Patent number: 10357769
    Abstract: A sample testing cartridge is usable to perform a variety of tests on a viscoelastic sample, such hemostasis testing on a whole blood or blood component sample. The cartridge includes a sample processing portion that is in fluid communication with a sample retention structure. A suspension, such as a beam, arm, cantilever or similar structure supports or suspends the sample retention portion relative to the sample processing portion in a unitary structure. In this manner, the sample retention portion may be placed into dynamic excitation responsive to excitation of the cartridge and correspondingly dynamic, resonant excitation of the sample contained within the sample retention portion, while the sample processing portion remains fixed. Observation of the excited sample yields data indicative of hemostasis. The data may correspond to hemostasis parameters such as time to initial clot formation, rate of clot formation, maximum clot strength and degree of clot lysis.
    Type: Grant
    Filed: March 21, 2017
    Date of Patent: July 23, 2019
    Assignee: Coramed Technologies, LLC
    Inventors: Peter Delmenico, Carlos G. Lopez-Espina, Gabriel Raviv
  • Patent number: 10350594
    Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
    Type: Grant
    Filed: March 5, 2018
    Date of Patent: July 16, 2019
    Assignee: Berkeley Lights, Inc.
    Inventors: Eric D. Hobbs, Justin K. Valley
  • Patent number: 10350593
    Abstract: A device for monitoring fluid media, and methods of manufacture thereof are disclosed. The monitoring device may be implemented as a single monolithic unit made from a certain type of material without a separate packaging, and having a base element comprising at least one fluid port and at least one cavity or fluid flow path fluidly coupled to the at least one fluid port for enabling fluid exchange of fluid media therewith. The device comprises at least one sensing element associated with the cavity or fluid flow path and configured and operable for measuring at least one property or condition of fluid media introduced thereinto, and generate measurement data or signals indicative thereof. Electrical contacts disposed on the base element of the device, and electrically coupled to the at least one sensing element are used for establishing electrical connection with the at least one sensing element.
    Type: Grant
    Filed: August 3, 2016
    Date of Patent: July 16, 2019
    Assignee: EZMEMS LTD.
    Inventors: Tsvi Shmilovich, Nicola Molinazzi
  • Patent number: 10343165
    Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.
    Type: Grant
    Filed: December 30, 2016
    Date of Patent: July 9, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Amir Sadri, Kun Guo, Daniel Y. Chu, Nenad Kircanski, Paul J. Patt, Tal Rosenzweig
  • Patent number: 10345317
    Abstract: A sensor including a surface plasmon resonance detector with a reservoir for containing a liquid sample. The sensor further includes a sensing metallic film positioned within the reservoir so that at least a majority of a surface of the sensing metallic film is to be in contact with the liquid sample being housed within the reservoir. The sensory also includes a semiconductor device having a contact in electrical communication with the sensing metal containing film that is positioned within the reservoir. The semiconductor device measures the net charges of molecules within the liquid sample within a Debye length from the sensing metallic film.
    Type: Grant
    Filed: April 26, 2017
    Date of Patent: July 9, 2019
    Assignee: International Business Machines Corporation
    Inventors: Bobby E. Feller, Jianqiang Lin, Robert D. Miller, Ramachandran Muralidhar, Tak H. Ning, Sufi Zafar
  • Patent number: 10335788
    Abstract: Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: July 2, 2019
    Assignee: Emulate, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Joshua Gomes, Kyung Jin Jang
  • Patent number: 10338071
    Abstract: A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5? and 3? ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3? ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample. Because the system is non-lytic, the testing can be repeated on the cells which remain viable in culture.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: July 2, 2019
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Stephen M. Hahn, Jay F. Dorsey, Gary D. Kao, Emigdio Reyes