Abstract: An assay system permits study of cell migration through tissue-relevant extracellular matrices under a chemokine gradient within a modular assay platform. Activated CD3+ T-cells were detected, quantified, and studied as they migrated towards chemokines (CXCL10 and CXCL12) and chemokine mimetics (CXCR3 agonist) using the assay system. Discovery of drugs, and the study of microenvironments, stimuli, and responding cells are facilitated by the assay system.

Abstract: Disclosed is a device for analyzing a single cell using a micropore including an inlet chamber; an outlet chamber provided on an opposite side of the inlet chamber; a pore membrane disposed between the inlet chamber and the outlet chamber; a pressure generating means provided in the inlet chamber or the outlet chamber; and a pair of electrodes respectively disposed in front and rear of the pore membrane, wherein a diameter D of the micropore is larger than a diameter of the target cell, a thickness t of the pore membrane is 0.5 ?m to 1 mm, and a slenderness ratio (t/D) is 0.001 to 5.

Abstract: Disclosed is an approach to differentiating between different particle types in samples flowing through microfluidics chips. A sample may have an initial proportion of a first cell type to a second cell type. An illuminating light source may emit a coherent light at the sample, and light leaving the chip in a first direction may be detected using a first light detector, and light leaving the chip in a second direction (e.g., orthogonal to the first direction) may be detected using a second light detector. The detected light may be fluorescence. An orientational feature of a plurality of cells in the sample may be determined based on the light detected by the detectors. Based on the orientational features and the detected light, a biasing operation may be performed for each cell in the sample to obtain a modified proportion of cell types in the sample.

Abstract: The present invention provides a chemical analyzer with highly reliable agitation performance, said chemical analyzer not only diagnosing the deterioration of a piezoelectric element, but also diagnosing the deformation and displacement of a reaction container and diagnosing the normality of the liquid quantity of a substance to be agitated in the reaction container. This chemical analyzer is characterized by comprising: an agitating mechanism that uses acoustic waves to agitate a sample and a reagent within a reaction container, generates acoustic waves using a piezoelectric element, and has an acoustic wave sensor for detecting the acoustic waves; and a controller that controls the agitating mechanism.

Abstract: The presently disclosed subject matter provides dual-depth thermoplastic microfluidic devices, related kits, microfluidic systems comprising the dual-depth thermoplastic microfluidic device, methods of isolating nucleic acid analytes from a liquid sample, and methods of isolating extracellular vesicles from a liquid sample.

Abstract: In embodiments, a silicon part and a titanium part may be soldered together without breakage or instability. In embodiments, silicon and titanium may be soldered together with a soft solder joint including indium silver, where the temperature excursion between solder solidus and use temperature limits the strain between the two surfaces. In embodiments a silicon micropump surface may be treated to remove its silicon oxide coating, and then Ti—W, Nickel, and gold layers successively sputtered onto it. A corresponding titanium manifold may be ground flat, and plated with electroless nickel. The nickel plated manifold may then be baked, so as to create a transition from pure Ti to Ni—Ti alloy to pure Ni at the surface of the manifold, and for protection of the upper Ni surface, a layer of gold may be added. The two surfaces may then be soldered in forming gas.

Abstract: The disclosure provides white blood cell classifying and counting method, blood analyzer, and computer readable storage medium. The method includes: obtaining a first histogram of white blood cells in a blood sample treated by a first hemolytic agent and a second histogram of white blood cells in the blood sample treated by a second hemolytic agent, wherein a ghost value in the second histogram is smaller than a ghost value in the first histogram, and the ghost value in the second histogram is smaller than a threshold value; determining peak type of the second histogram; when the peak type is double-peak, performing classification and counting of white blood cells using the second histogram; and when the peak type is single-peak, performing classification and counting of white blood cells by combining a classification result and a counting result of the second histogram and a classification result of the first histogram.

Abstract: A microfluidic system configured to focus particles suspended in a fluid. One general aspect includes a microfluidic system comprising one or more substrates and a focusing channel formed in the one or more substrates and spanning a length from an inlet to an outlet for receiving a flow of particles suspended in fluid, wherein the particles have a diameter (a) and the focusing channel has a hydraulic diameter (dh).

Abstract: The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w/3 to about ¾w, and wherein the flow cell has a height of between about 1/12w to about ?w.

Abstract: The present disclosure provides a carbon-based oxygen-enriched combustion method for recirculation of flue gas from a cement kiln. The combustion method includes recirculating the flue gas generated by the cement kiln to a certain degree to concentrate and enrich carbon dioxide in the flue gas of the cement kiln, mixing the carbon dioxide-rich recirculating flue gas of the cement kiln with the pressurized oxygen to obtain the carbon-based oxygen-enriched products, and mixing the carbon-based oxygen-enriched products with the atmospheric-pressure oxygen to obtain carbon-based air which serves as combustion-supporting gas of the cement kiln.

Abstract: In one example in accordance with the present disclosure, a fluid ejection die is described. The fluid ejection die includes a fluid feed slot to deliver fluid from a reservoir to an array of ejection chambers fluid connected to the fluid feed slot. Each ejection chamber includes at least one fluid actuator and an opening through which fluid is to be ejected. The fluid ejection die also includes a number of antechambers. An antechamber includes sidewalls that curve inward.

Abstract: The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

Abstract: Provided is an automatic analyzer to control a temperature of a reagent supplied to a magnetic separator with high accuracy. The automatic analyzer includes a magnetic separator that separates a magnetic component and a non-magnetic component from liquid obtained by reacting a sample with a first reagent, a storage unit that accommodates a second reagent, a pipe used to supply the second reagent to the magnetic separator, and a heat exchanger that adjusts a temperature around the pipe. It is preferable that the heat exchanger includes a heat absorption and dissipation unit and a fan that blows air heat-exchanged by the heat absorption and dissipation unit, and the air blown from the fan is blown onto the pipe.

Abstract: The techniques relate to methods and apparatus for sealed fluid chambers. The device includes a sensor chip comprising a set of sensor elements at least partially disposed on a first side of a substrate, wherein each sensor element of the set of sensor elements is configured to sense an analyte and comprises an associated set of through silicon vias (TSVs), each TSV of the set of TSVs extending from an associated portion of the sensor element through the substrate to a second side of the substrate that is opposite the first side. The device includes a fluid chamber proximate to the first side of the substrate and comprising an inner portion in fluid communication with the set of sensor elements, and a sealing member between the fluid chamber and the first side of the substrate.

Abstract: The compounds disclosed herein (e.g., compounds having a structure according to Formula (I/(II)/(III)/(IV), and (V)) are polymers wherein an organic polymeric segment (e.g., a polyethylene glycol (PEG) group) comprises covalent attachments to two or more lipid substructures, and each lipid substructure independently comprises a hydrophobic moiety and a hydrophilic moiety. The compounds provided herein can be useful for delivery and expression of mRNA and encoded protein, e.g., as a component of liposomal delivery vehicle, and accordingly can be useful for treating various diseases, disorders and conditions, such as those associated with deficiency of one or more proteins.

Abstract: Provided are methods, devices, and systems for identifying the concentration of delta-9-tetrahydrocannabinol (?-9 THC) in a given sample. The system allows for in situ formation of a chromophore eliminating the use of chloroform, wherein said chromophore absorbance spectra is measured using a spectroscopy method within a light wavelength range. The system allows for calculating the concentration of ?-9 THC using the linear relationship between the ?-9 THC level and the intensity of the color change in the present tester. Said linear relationship is obtained because the tester of the present disclosure allows the chromophore formation reaction go to completion.

Abstract: The present disclosure provides nanoparticle transducers and methods of use thereof for the detection of analyte concentrations in a fluid. Nanoparticle transducers can comprise a nanoparticle, such as a Pdot, coupled to an enzyme that catalyzes a reaction with the analyte. The nanoparticle transducers further comprise chromophores that emit fluorescence that varies as a function of the concentration of one of the elements of the reaction. The nanoparticle transducer thus changes fluorescence as the analyte concentration changes, transforming analyte concentration values into fluorescence intensities. The measurement of these intensities provides a measurement of the analyte concentration. The nanoparticle transducers are biocompatible, allowing for use in vivo, for the monitoring of analyte blood concentrations such as blood glucose concentrations.

Abstract: Provided are methods for determining the apolipoprotein E (ApoE) phenotype in a sample by mass spectrometry; wherein the ApoE allele(s) present in the sample is determined from the identity of the ions detected by mass spectrometry. In another aspect, provided herein are methods for diagnosis or prognosis of Alzheimer's disease or dementia.

Abstract: An integrated testing device and fluid module are disclosed, as well as a method of manufacture. Fluid module contains a reservoir containing a test fluid, and a control vessel. The reservoir discharges test fluid into the control vessel, which discharges the test fluid in a controlled way to a test component.

Abstract: An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.