Patents Examined by Catherine S Hibbert
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Patent number: 11701391Abstract: Methods of treating an ischemic disease in a subject are provided. Accordingly there is provided a method comprising administering to the subject a therapeutically effective amount of cells with reduced level of expression and/or activity of TNFR1, thereby treating the ischemic disease in the subject. Also provided is a method comprising treating with TNFalpha cells with reduced expression and/or activity of TNFR1 and administering to the subject a therapeutically effective amount of said cells, thereby treating the ischemic disease in the subject.Type: GrantFiled: October 24, 2018Date of Patent: July 18, 2023Inventor: Dalia Elani
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Patent number: 11697827Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.Type: GrantFiled: December 9, 2020Date of Patent: July 11, 2023Assignee: Synthego CorporationInventors: Richard Stoner, Travis Maures, David Conant
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Patent number: 11692213Abstract: Provided herein are methods and compositions for depleting targeted nucleic acid sequences from a sample, enriching for sequences of interest from a sample, and/or partitioning of sequences from a sample. The methods and compositions are applicable to biological, clinical, forensic, and environmental samples.Type: GrantFiled: August 3, 2020Date of Patent: July 4, 2023Assignee: ARC BIO, LLCInventors: Meredith L. Carpenter, Carlos D. Bustamante, Stephane B. Gourguechon
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Patent number: 11692193Abstract: Recombinant expression vectors are disclosed that include a control sequence for recombinant expression of proteins of interest; the control sequence combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Some of the vectors are useful for tetracycline-inducible expression. Some of the vectors contain a 5? PiggyBac ITR and a 3? PiggyBac ITR to promote genomic integration into a host cell chromosome. A method of selecting a stable production cell line for manufacturing a protein of interest is also disclosed. Also disclosed are mammalian host cells comprising the inventive recombinant expression vectors and a method of producing a protein of interest, in vitro, involving the mammalian host cell.Type: GrantFiled: March 3, 2021Date of Patent: July 4, 2023Assignee: Just-Evotec Biologies, Inc.Inventors: Jeffrey T. McGrew, Pauline S. Smidt, E-Ching Ong
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Patent number: 11685943Abstract: Provided herein are methods for selecting a population of cells expressing a target polypeptide. In some aspects, the disclosure provides methods for sorting and selecting populations of transfected host cells based on their early expression of a selectable polypeptide. In certain embodiments, the sorting is performed using fluorescence-activated cell sorting or magnetic-activated cell sorting based on the selectable polypeptide. Such selection methods can be further utilized to generate clonal populations of producer cells, e.g. for large-scale manufacturing of a target polypeptide of interest.Type: GrantFiled: November 14, 2019Date of Patent: June 27, 2023Assignee: GENZYME CORPORATIONInventors: Victor R. Cairns, Christine DeMaria, Jason Vitko
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Patent number: 11684679Abstract: An isolated recombinant parvovirus vector comprising a synthetic enhancer comprising plurality of enhancer sequences operably linked to a promoter, and methods of using the vector, are provided.Type: GrantFiled: March 7, 2017Date of Patent: June 27, 2023Assignee: University of Iowa Research FoundationInventors: John F. Engelhardt, Ziying Yan
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Patent number: 11685933Abstract: Recombinant expression vectors are disclosed that include a control sequence for recombinant expression of proteins of interest; the control sequence combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Some of the vectors are useful for tetracycline-inducible expression. Some of the vectors contain a 5? PiggyBac ITR and a 3? PiggyBac ITR to promote genomic integration into a host cell chromosome. A method of selecting a stable production cell line for manufacturing a protein of interest is also disclosed. Also disclosed are mammalian host cells comprising the inventive recombinant expression vectors and a method of producing a protein of interest, in vitro, involving the mammalian host cell.Type: GrantFiled: February 1, 2022Date of Patent: June 27, 2023Assignee: Just-Evotec Biologics, Inc.Inventors: Jeffrey T. McGrew, Pauline S. Smidt, E-Ching Ong
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Patent number: 11680253Abstract: Methods for labeling and imaging the accessible genome using a transposase are disclosed. In some embodiments, a bifunctional transposase complex or transposome is used to insert adaptors comprising chemical tags selectively at accessible sites in the genome where active regulatory DNA is located. Various chemical tags can be used for labeling DNA at insertion sites, including, for example, fluorescent dyes for fluorescence imaging, metal particles for electron microscopy or magnetic manipulation of DNA, isotopic labels, or biotin or other ligands, haptens, substrates, or inhibitors that are recognized by streptavidin, antibodies, enzymes, or receptors. Labeling DNA in this manner can be used to provide spatial information regarding the positioning of regulatory DNA in the genome and makes possible the imaging and sorting of cells based on the status of their regulatory DNA.Type: GrantFiled: March 9, 2017Date of Patent: June 20, 2023Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Howard Y. Chang, William J. Greenleaf, Xingqi Chen, Jason Buenrostro
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Patent number: 11680274Abstract: A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences.Type: GrantFiled: March 28, 2019Date of Patent: June 20, 2023Assignee: The Trustees of the University of PennsylvaniaInventors: Luk Vandenberghe, Guangping Gao, James M. Wilson
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Patent number: 11672855Abstract: Disclosed is an adapted Madin-Darby canine kidney cell line capable of suspension culture in the absence of serum, and a chemically-defined medium for culture of the adapted MDCK cell line. Further disclosed are culture methods for growing the adapted MDCK cell line and methods for producing a vaccine from the adapted MDCK cell line grown in the chemically-defined medium.Type: GrantFiled: September 28, 2020Date of Patent: June 13, 2023Assignees: NATIONAL HEALTH RESEARCH INSTITUTES, FUJIFILM IRVINE SCIENTIFIC, INC.Inventors: Jenny Bang, Hsiao-Tzu Ni, Alan Yung-Chih Hu, Tsai-Chuan Weng
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Patent number: 11649469Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.Type: GrantFiled: May 14, 2019Date of Patent: May 16, 2023Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, Kevin M. Esvelt, Prashant G. Mali
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Patent number: 11640849Abstract: The present disclosure provides a diagnostic method based on pairwise comparison of cancers using transcriptome expression data. In one embodiment, the method comprises the steps of: obtaining a first gene expression profile of a first cancer sample having a first cancer type; obtaining a second gene expression profile of a second cancer sample having a second cancer type, wherein the second cancer type is different from the first cancer type; comparing said first gene expression profile with said second gene expression profile; and selecting N genes that are most differentially expressed in the first and the second gene expression profiles to generate pairwise differentially expressed genes (DEGs), wherein N is an integer between 10 and 100.Type: GrantFiled: September 9, 2016Date of Patent: May 2, 2023Assignee: CROWN BIOSCIENCE, INC. (TAICANG)Inventors: Sheng Guo, Henry Qixiang Li
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Patent number: 11617711Abstract: Disclosed herein are compositions and methods for their use in treating dental disorders, whitening teeth, restoring and retaining the tooth structure and restoring the mineral content of the tooth-mineral loss due to demineralization, wherein the compositions include a first formulation that includes a polypeptide effective to treat a dental disorder and/or whiten teeth, and one or more further formulations including at least one calcium ion source and/or at least one phosphate ion source, wherein the first formulation is configured to release the polypeptide more rapidly than Ca2+ is released from the at least one calcium ion source and PO43? is released from the at least one phosphate ion source.Type: GrantFiled: August 30, 2019Date of Patent: April 4, 2023Assignee: UNIVERSITY OF WASHINGTONInventors: Mehmet Sarikaya, Deniz Tanil Yucesoy, Hanson Kwok Fong, Sami Dogan
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Patent number: 11603527Abstract: The present invention relates to a method for purification of viral vectors, more closely it relates to purification of viral vectors from producer cells by using a single automated process. The method comprises the following steps: a) adding producer cells and cell lysis buffer to a processing container; b) mixing said producer cells and cell lysis buffer in said processing container to obtain a mixture; c) flowing said mixture through a chromatography column for purification of viral vectors, wherein the viral vectors are adsorbed on said chromatography column; and d) eluting viral vectors from the chromatography column into a product container.Type: GrantFiled: December 27, 2017Date of Patent: March 14, 2023Assignee: Global Life Sciences Solutions USA LLCInventor: Trevor Smith
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Patent number: 11591382Abstract: The present invention provides compositions comprising a Fc-containing protein wherein substantially all the Fc domains have a C-terminal lysine. Further provided are host cell for producing said compositions, methods of making said host cells and compositions, and method of use thereof.Type: GrantFiled: September 21, 2016Date of Patent: February 28, 2023Assignee: Genentech, Inc.Inventors: Benjamin Haley, Zhilan Hu, John C. Joly, Amy Y. Shen, Bradley Richard Snedecor
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Patent number: 11571483Abstract: A modified melanoma cell line capable of quantification of the effects of MEK inhibitors and CDK4/6 inhibitors in a quantitative, temporal and non-invasive manner both in vitro and in vivo.Type: GrantFiled: September 3, 2020Date of Patent: February 7, 2023Assignee: THOMAS JEFFERSON UNIVERSITYInventors: Andrew Aplin, Jessica Teh
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Patent number: 11566052Abstract: The invention relates to methods of reducing the immunogenicity of CRISPR-associated (Cas) proteins and the modified Cas proteins produced therefrom. In addition, the invention relates to methods for cell and gene therapy, including any and all genetic modifications and alterations of gene expression (and/or genetic elements) made in-vivo or ex-vivo using Cas proteins with reduced immunogenicity.Type: GrantFiled: November 11, 2016Date of Patent: January 31, 2023Assignee: LONZA LTD.Inventors: Thomas Payne, Jesús Zurdo, Noel Hillier Smith
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Patent number: 11555181Abstract: The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.Type: GrantFiled: September 21, 2020Date of Patent: January 17, 2023Assignee: Caribou Biosciences, Inc.Inventors: Peter Sean Cameron, Sanne Eveline Klompe, Samuel Henry Sternberg
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Patent number: 11549119Abstract: The present disclosure relates to vectors for cloning and expressing genetic material including but not limiting to antibody gene or parts thereof and methods of generating said vectors. Said vectors express the antibody genes in different formats such as Fab or scFv as a part of intertransfer system, intratransfer system or direct cloning and expression in individual display systems. In particular, phage display technology is used to clone and screen potential antibody genes in phagemid which is followed by the transfer of said genes to yeast vector for further screening and identification of lead molecules against antigens. The present vectors have numerous advantages including uniquely designed inserts/expression cassettes resulting in efficient and smooth transfer of clonal population from phage to yeast vectors resulting in efficient library preparation and identification of lead molecules.Type: GrantFiled: April 6, 2017Date of Patent: January 10, 2023Assignee: ZUMUTOR BIOLOGICS, INC.Inventors: Sohang Chatterjee, Kavitha Iyer Rodrigues, Maloy Ghosh, Sunit Maity, Divya Unnikrishnan, Yogendra Manjunath Bangalore Muniraju, Sathyabalan Murugesan, Pavithra Mukunda, Bhargav Prasad, Veeresha Kamanagowda, Sanghamitra Bhattacharjee, Pravin Kumar Dakshinamurthy, Vivek Halan, Sankaranarayanan Srinivasan, Anuradha Hora, Bairavabalakumar Natarajan, Karthika Nair, Aswini Thanigaivel, Amol Maliwalave, Bharath Ravindra Shenoy, Sahana Bhima Rao, Subhra Prakash Chakrabarty, Ashvini Kumar Dubey, Amir Khan, Ankurina Sharma, Rashmi Sharma, Anurag Tiwari, Santosh Kumar, Shivani Patel, Nikitha M
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Patent number: 11548937Abstract: The present disclosure relates to a method of obtaining a cell where fucosylation pathways are modified, leading to production of partially fucosylated and non-fucosylated protein products, specifically antibodies from the cell. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology. The method of the present disclosure targets the Fut8 gene and GMD gene in a cell. Such products are used in developing therapeutics and biomarkers, and in diagnosis and prognosis of diseases.Type: GrantFiled: July 6, 2020Date of Patent: January 10, 2023Assignee: Zumutor Biologics Inc.Inventors: Bhargav Prasad, Divya Unnikrishnan, Jahnabi Hazarika, Kavitha Iyer Rodrigues, Maloy Ghosh, Pavithra M, Pravin Kumar D, Sanghamitra Bhattacharjee, Sathyabalan M, Sankaranarayanan Srinivasan, Sohang Chatterjee, Sunit Maity, Veeresha K, Vivek Halan, Yogendra Manjunath B. M., Anuradha Hora, Bairavabalakumar N, Karthika Nair, Aswini Thanigaivel, Amol Maliwalave, Bharath R Shenoy, Rajeshwari Pendse, Prabhat Kumar Pathak, Anisha Kurup, Sahana Bhima Rao