Patents Examined by Channing S. Mahatan
  • Digital counting of individual molecules by stochastic attachment of diverse labels
    Patent number: 9816137
    Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: November 14, 2017
    Assignee: Cellular Research, Inc.
    Inventors: Stephen P. A. Fodor, Glenn K. Fu
  • Method of using salmon thrombin to alleviate central nervous system-mediated pain
    Patent number: 9808514
    Abstract: A method of alleviating central nervous system-mediated pain includes applying salmon thrombin at a neural injury site. Applying salmon thrombin can include applying a gel that includes salmon thrombin. The gel can also include fibrinogen, for example, salmon fibrinogen, human fibrinogen, or bovine fibrinogen. The salmon thrombin can be obtained from salmon plasma, or using recombinant technology, or by fractionation. A pain relief substance includes a gel that includes salmon thrombin.
    Type: Grant
    Filed: October 20, 2009
    Date of Patent: November 7, 2017
    Assignee: Sea Run Holdings, Inc.
    Inventors: Beth A. Winkelstein, Paul A. Janmey, Evelyn S. Sawyer
  • Methods and kits for diagnosing osteoarthritis and predicting progression
    Patent number: 9804175
    Abstract: This disclosure relates to methods and kits for diagnosing osteoarthritis and for determining the progression of osteoarthritis in a subject.
    Type: Grant
    Filed: November 17, 2014
    Date of Patent: October 31, 2017
    Assignee: New York University
    Inventors: Mukundan Attur, Steven B. Abramson
  • Biomarkers for hypertensive disorders of pregnancy
    Patent number: 9791457
    Abstract: The application discloses new biomarkers for hypertensive disorders of pregnancy and particularly preeclampsia; methods for the diagnosis, prediction, prognosis and/or monitoring said disorders based on measuring said biomarkers; and kits and devices for measuring said biomarker and/or performing said methods.
    Type: Grant
    Filed: November 3, 2015
    Date of Patent: October 17, 2017
    Assignee: MYCARTIS NV
    Inventor: Koen Kas
  • Efficient biomolecule recycling method and system
    Patent number: 9765396
    Abstract: A technique is disclosed for recapturing and recycling biomolecule reagents. The technique may be applied in a range of settings, including biopolymer synthesis, sequencing, and so forth. Biomolecule reagents such as nucleotides and oligonucleotides used to process nucleic acids, which may be marked with fluorescent tags, carry blocking agents, and so forth, are introduced to samples in a sample container. After the desired reaction occurs with some of the biomolecule reagents, such as some of the nucleotides or oligonucleotides, the effluent stream is processed to recapture unreacted biomolecule reagents. These may be separated from other reaction components, and recycled into the same or a different sample container. The recaptured biomolecule reagents may be mixed with additional biomolecule reagents prior to reintroduction to the same or different samples.
    Type: Grant
    Filed: March 22, 2016
    Date of Patent: September 19, 2017
    Assignee: Illumina, Inc.
    Inventor: Chanfeng Zhao
  • Methods and compositions for the diagnosis and prognosis of Alzheimer's disease
    Patent number: 9726678
    Abstract: The invention relates to methods of diagnosing Alzheimer's disease (AD) in a subject and methods of determining the prognosis in patients with AD. The adhesion molecules P-selectin and L-selectin are described for the first time for use as biomarkers to aid in the diagnosis of AD. The invention further describes the use of one or more of L-selectin, MCP-1, IL-1?, IL-8 and IFN-? to aid in the prognosis of either accelerated cognitive decline (ACD) or slow cognitive decline (SCD) in patients with AD.
    Type: Grant
    Filed: April 15, 2010
    Date of Patent: August 8, 2017
    Assignee: RANDOX LABORATORIES LTD.
    Inventors: Massimiliano Corsi, Stephen Peter Fitzgerald, John V. Lamont, Paul Innocenzi
  • Digital counting of individual molecules by stochastic attachment of diverse labels
    Patent number: 9708659
    Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
    Type: Grant
    Filed: November 12, 2014
    Date of Patent: July 18, 2017
    Assignee: Cellular Research, Inc.
    Inventors: Stephen P. A. Fodor, Glenn K. Fu
  • Systems, methods and compositions for optimizing tissue and cell enriched grafts
    Patent number: 9695398
    Abstract: Disclosed herein are methods and systems for the concentration of cells from a cell suspension into unprocessed tissue, such as adipose tissue. Also disclosed herein are systems for optimizing hydration of tissue and cell enriched grafts.
    Type: Grant
    Filed: August 18, 2015
    Date of Patent: July 4, 2017
    Assignee: BIMINI TECHNOLOGIES LLC
    Inventors: Alvin Peterson, Lucas Fornace
  • Specific and high affinity binding proteins comprising modified SH3 domains of Fyn kinase
    Patent number: 9689879
    Abstract: The present invention relates to a method for the production of a library comprising recombinant derivatives of the SH3 domain of the Fyn kinase of SEQ ID NO: 1 as well as a method for selecting from a library comprising recombinant derivatives of the SH3 domain of the Fyn kinase of SEQ ID NO: 1 one or more of said derivatives having a specific binding affinity to a protein or peptide.
    Type: Grant
    Filed: August 31, 2016
    Date of Patent: June 27, 2017
    Assignee: EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZURICH
    Inventors: Dragan Grabulovski, Dario Neri
  • Artificial nucleic acid molecules for improved protein or peptide expression
    Patent number: 9683233
    Abstract: The invention relates to an artificial nucleic acid molecule comprising at least one 5?UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and/or a 3?UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: June 20, 2017
    Assignee: CureVac AG
    Inventor: Andreas Thess
  • Glucose and insulin sensors and methods of use thereof
    Patent number: 9683266
    Abstract: Described herein are glucose and insulin sensors. The sensors are composed of host cells with DNA specifically designed to produce fluorescence when the cells come into contact with glucose and/or insulin in the sample. Once the fluorescence has been quantified, it can be correlated with the amount of glucose and/or insulin present in the sample.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: June 20, 2017
    Assignee: INTERNATIONAL PARK OF CREATIVITY
    Inventors: Raul Cuero Rengifo, Juliana Maria Navia
  • Multiple interferon and virus response element cell-based fluorescence system
    Patent number: 9677071
    Abstract: The present disclosure refers to a method for a specific, versatile and sensitive detection of IFN-/virus-induced genes, a method for quantifying IFN potency and activity in a pharmaceutical preparation or biological sample, a method for distinguishing between IFN- and viral induction, and/or for distinguishing between different viruses, and a method for the quantification of virus activity. Also, the invention provides the necessary molecular tools like expression active response constructs, suitable cell lines, an array to perform the method and a kit.
    Type: Grant
    Filed: April 20, 2011
    Date of Patent: June 13, 2017
    Assignees: KING FAISAL SPECIALIST HOSPITAL AND RESEARCH CENTRE, KING SAUD UNIVERSITY
    Inventors: Khalid S. A. Khabar, Fahad Nasser Al-Majhdi
  • Method of eliminating dependence of methanol induced promoter on single methanol carbon source
    Patent number: 9677080
    Abstract: Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.
    Type: Grant
    Filed: November 15, 2012
    Date of Patent: June 13, 2017
    Assignee: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Xiangshan Zhou, Yuanxing Zhang, Jinjia Wang, Xiaolong Wang, Peng Bai, Ping Zhang
  • strains producing greatly increased yield of PHB-deficient sphingan (diutan)
    Patent number: 9677100
    Abstract: PHB-deficient Sphingomonas strains having improved sphingan yield are provided. Certain of the Sphingomonas strains are diutan-producing strains that exhibit a dramatic improvement in productivity and yield due to a combination of certain genetic modifications that affect PHB and sphingan synthesis. Moreover, the sphingans produced from such strains have superior characteristics including improved filterability, clarity, and improved rheology-modifying characteristics. The sphingans provided are, thus, highly desirable in a variety of commercial and industrial uses, including personal care items, cement applications, and oilfield applications.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: June 13, 2017
    Assignee: CP Kelco U.S., Inc.
    Inventors: Nancy E. Harding, Todd A. Talashek, Yamini N. Patel
  • Low-phosphate repressible promoter
    Patent number: 9670493
    Abstract: The present invention provides compositions and methods comprising a low-phosphate repressible promoter. In particular, the present invention provides a low-phosphate repressible promoter from E. coli.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: June 6, 2017
    Assignee: Codexis, Inc.
    Inventors: Fernando Valle, Patricia Choudhary, Robert Osborne, Saul Nitsche Rocha, Monica Bhatia, U Loi Lao, Yihui Zhu
  • Genome-wide construction of heterozygous deletion mutants containing gene-specific barcodes by the methods of 4-round serial or block PCR, or total gene synthesis thereof
    Patent number: 9670480
    Abstract: A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: June 6, 2017
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Kwang Lae Hoe, Dong Uk Kim, Mi Sun Won, Hyang Sook Yoo, Dong Sup Kim, Han Oh Park, Kyung Sook Chung, Young Joo Jang, Mi Young Nam, Sang Jo Han, Shin Jung Choi, Seung Tae Baek, Hyong Bai Kim, Kyung Sun Heo, Hye Mi Lee, Min Ho Lee, Jo Young Park
  • Nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen
    Patent number: 9669089
    Abstract: The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a pathogenic antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of infectious diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising a pathogenic antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.
    Type: Grant
    Filed: February 15, 2013
    Date of Patent: June 6, 2017
    Assignee: CureVac AG
    Inventors: Andreas Thess, Thomas Schlake, Jochen Probst
  • Mitochondrial expression vector and method for the transformation of mitochondria
    Patent number: 9670505
    Abstract: The invention relates to a mitochondrial expression vector which may comprise a gene to be expressed and/or a selection marker and a mitochondrial region, and a method for inserting a DNA to be expressed into mitochondria of mammalian cells, wherein the method may comprise the steps: (i) construction of a mitochondrial expression vector or a mitochondrial genome, (ii) reversible induction of megamitochondria and (iii) transfection of the megamitochondria by means of a physical transfection method.
    Type: Grant
    Filed: March 26, 2015
    Date of Patent: June 6, 2017
    Assignee: UNIVERSITAT LEIPZIG
    Inventor: Peter Seibel
  • Pan-yeast autonomously replicating sequence
    Patent number: 9670495
    Abstract: A DNA sequence that functions as an origin in many different yeast species. From 1 to 17 mutations can be introduced into this sequence to improve its function across multiple yeasts. The resulting synthetic DNA sequence confers stable plasmid replication function in all yeast species tested, including but not limited to Saccharomyces cerevisiae, Lachancea kluyveri, Kluyveromyces lactis, Kluyveromyces wickerhammii, Hansenula polymorpha, and Pichia pastoris. Also provided are sequences that function as an optimal origin in the industrially useful Pichia pastoris.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: June 6, 2017
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Maitreya Dunham, Ivan Liachko
  • Method for the production of recombinant polyomaviral vector particles
    Patent number: 9587226
    Abstract: The present invention relates to improved methods for the production of viral particles, viral vector particles and recombinant proteins. In particular, the invention relates to improved methods for the production of recombinant polyomaviral vector particles and polyomaviral vector production cell lines. More in particular, the invention relates to methods for the production of simian polyomaviral vector particles such as simian virus 40 (SV40) viral vector particles. The invention also relates to compositions comprising viral vectors and uses thereof and viral vector particles to treat genetic disorders, transplant rejection, autoimmune diseases, infectious diseases, allergies or cancer. The invention also relates to methods for the production of recombinant proteins in mammalian cells and methods to enhance the production of recombinant proteins in mammalian cells.
    Type: Grant
    Filed: April 22, 2010
    Date of Patent: March 7, 2017
    Assignee: Amarna Holding B.V.
    Inventor: Walter Gerhardus De Vries