Patents Examined by Channing S. Mahatan
  • High-secretion heat-resistant yeast genetically engineered strain and application thereof
    Patent number: 10519417
    Abstract: The invention discloses a high-secretion heat-resistant yeast genetically engineered strain and application thereof, belonging to the field of biotechnology. Mutagenesis and domestication are performed to obtain the yeast genetically engineered strain capable of expressing a lipase gene at a high secretion level at high temperature. The strain is collected by China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO: M 2016278. The sequencing analysis shows that the lipase gene and promoter sequence thereof are not mutated, which indicates that the high-secretion expression of the lipase gene by the mutant strain is caused by mutation of other gene sequences in the genome.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: December 31, 2019
    Assignee: Jiangnan University
    Inventors: Xiaowei Yu, Yan Xu
  • Compounds and methods for modulating frataxin expression
    Patent number: 10517877
    Abstract: The present technology relates to compositions and methods for modulating expression of genes, which include a target oligonucleotide sequence, such as repeats of a particular oligonucleotide sequence containing 3 to 10 nucleotides. In particular aspects, the present technology relates to agents having a formula A-L-B, wherein -L- is a linker; A- is a Brd4 binding moiety; and -B is a nucleic acid binding moiety, such as a polyamide or complementary oligonucleotide, that specifically binds to the target oligonucleotide sequence.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: December 31, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Aseem Ansari, Graham Erwin, Matthew Grieshop
  • Discovering and producing conditionally active biologic proteins in the same eukaryotic cell production hosts
    Patent number: 10513699
    Abstract: A method of preparing a conditionally active biologic protein by selecting a wild-type biologic protein, evolving the DNA which encodes the wild-type biologic protein using one or more evolutionary techniques to create mutant DNAs, expressing the mutant DNAs in a eukaryotic cell production host to obtain a mutant protein, subjecting the mutant protein and the wild-type protein to an assay under a normal physiological condition and to an assay under an aberrant condition, selecting a conditionally active mutant protein which exhibits at least one of: (a) a decrease in activity in the assay at the normal physiological condition compared to the wild-type protein, and (b) an increase in activity in the assay under the aberrant condition compared to the wild-type protein; and producing the conditionally active biologic protein in the same eukaryotic cell production host used in the expression step.
    Type: Grant
    Filed: September 3, 2015
    Date of Patent: December 24, 2019
    Assignee: BioAtla, LLC
    Inventor: Jay M. Short
  • Glucose sensors and methods of use thereof
    Patent number: 10501815
    Abstract: Described herein are glucose and insulin sensors. The sensors are composed of host cells with DNA specifically designed to produce fluorescence when the cells come into contact with glucose and/or insulin in the sample. Once the fluorescence has been quantified, it can be correlated with the amount of glucose and/or insulin present in the sample.
    Type: Grant
    Filed: April 2, 2018
    Date of Patent: December 10, 2019
    Assignee: International Park of Creativity
    Inventor: Raul Cuero Rengifo
  • One-pot multiplex gene synthesis
    Patent number: 10487323
    Abstract: The present invention provides methods for generating a library of synthetic polynucleotides. The present invention also provides methods for generating proteins encoded by the library of synthetic polynucleotides. In addition, provided herein are methods for determining the soluble expression of said proteins. This invention is based, in part, on the discovery of a method for selecting optimal oligonucleotides in combination with performing a phosphorylation reaction, ligation reaction and PCR amplification in a single reaction vessel to produce synthetic polynucleotides in a multiplex manner.
    Type: Grant
    Filed: August 3, 2015
    Date of Patent: November 26, 2019
    Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, AGILENT TECHNOLOGIES, INC.
    Inventors: John Christopher Anderson, Bo Curry, Timothy Hsiau
  • Administration regime
    Patent number: 10487364
    Abstract: A method for determining a folate substance administration regime is disclosed. The method comprises: quantifying, in a sample drawn from a patient, the expression level of at least one of the genes SLC46A1, SLC19A1, FPGS, ABCC3, MTHFD1 L, GGH, MTHFD1, MTFMT, and ATIC; and establishing whether the expression level is high or low. A high expression level of at least one of said genes determines that said folate substance administration regime involves the administration of [6R]-methylenetetrahydrofolate and/or a folate substance upstreams of [6R]-methylenetetrahydrofolate in the metabolic pathway. A low expression level of at least one of said genes determines that said folate substance administration regime involves the administration of [6R]-methylenetetrahydrofolate. Also disclosed is a kit for determining such a folate substance administration regime.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: November 26, 2019
    Assignee: ISOFOL MEDICAL AB
    Inventors: Yvonne Wettergren, Elisabeth Odin
  • Translational dysfunction based therapeutics
    Patent number: 10472677
    Abstract: Provided are methods and compositions for inhibiting eukaryotic translation initiation factor eIF4E. Such methods and compositions may be used alone or in conjunction with other therapies, such as gene therapies, for inhibiting cell proliferation and/or treating cancer.
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: November 12, 2019
    Assignee: Translational Therapeutics, Inc.
    Inventors: Gordon A. Jamieson, Jr., Katherine L. B. Borden, Biljana Culjkovic, Alex Kentsis
  • Multipurpose host system for invasive cardiovascular diagnostic measurement acquisition and display
    Patent number: 10463263
    Abstract: A multifunctional invasive cardiovascular diagnostic measurement host is disclosed that interfaces a variety of sensor devices, such as guide wire-mounted pressure sensors, flow sensors, temperature sensors, etc, and provides a multi-Mode graphical user interface providing a plurality of displays in accordance with the various types of sensors and measurements rendered by the sensors.
    Type: Grant
    Filed: July 18, 2016
    Date of Patent: November 5, 2019
    Assignee: VOLCANO CORPORATION
    Inventors: Howard David Alpert, Paul Michael Hoseit
  • Biopharmaceutical production method
    Patent number: 10457948
    Abstract: The present invention resides in a method for the manufacture of a disulphide-requiring biopharmaceutical having an element of at least tertiary structure using wild type E. coli.
    Type: Grant
    Filed: November 6, 2015
    Date of Patent: October 29, 2019
    Assignee: University of Kent
    Inventor: Colin Robinson
  • Methods and compositions for classification of samples
    Patent number: 10446272
    Abstract: Disclosed herein are kits, compositions, and methods relating to the classification of samples. Methods disclosed herein can be used to identify sample mix-ups. Methods disclosed herein can also be used to diagnose conditions or to support treatment-related decisions.
    Type: Grant
    Filed: September 12, 2017
    Date of Patent: October 15, 2019
    Assignee: Veracyte, Inc.
    Inventors: Jonathan I. Wilde, Darya Chudova, Giulia C. Kennedy
  • Increasing cellular lipid production by increasing the activity of diacylglycerol acyltransferase and decreasing the activity of triacylglycerol lipase
    Patent number: 10443047
    Abstract: Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by up-regulating diacylglycerol acyltransferase and down-regulating triacylglycerol lipase. In some embodiments, a DGA1 protein is expressed and a native TGL3 gene is knocked out, thereby increasing the synthesis of triacylglycerol and decreasing its consumption, respectively.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: October 15, 2019
    Assignee: Novogy, Inc.
    Inventors: Vasiliki Tsakraklides, Elena E. Brevnova
  • Gene expression profiles associated with sub-clinical kidney transplant rejection
    Patent number: 10443100
    Abstract: By a genome-wide gene analysis of expression profiles of over 50,000 known or putative gene sequences in peripheral blood, the present inventors have identified a consensus set of gene expression-based molecular biomarkers associated with subclinical acute rejection (subAR). These genes sets are useful for diagnosis, prognosis, monitoring of subAR.
    Type: Grant
    Filed: November 22, 2016
    Date of Patent: October 15, 2019
    Assignee: The Scripps Research Institute
    Inventors: Daniel R. Salomon, John Friedewald, Sunil Kurian, Michael M. Abecassis, Steve Head, Phillip Ordoukhanian
  • Methods of classifying periorbital dyschromia and systems therefor
    Patent number: 10405795
    Abstract: A method and system for classifying different types of periorbital dyschromia is disclosed. The method includes providing at least one of a predetermined imaging characteristic and a biological characteristic for each of three different types of periorbital dyschromia, and then measuring the appropriate characteristic on a person exhibiting periorbital dyschromia. The type of periorbital dyschromia exhibited by a person can then be determined by comparing the measured value to the predetermined value and selecting the corresponding type of periorbital dyschromia.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: September 10, 2019
    Assignee: The Procter & Gamble Company
    Inventors: Karen Marie Osorio, Karen Kay Kalla, Wenzhu Zhao, Bradley Bryan Jarrold
  • Oligonucleotide sequence for use in pathway engineering
    Patent number: 10400244
    Abstract: The present invention comprises a novel artificial oligonucleotide sequence which can initiate the transcription of a gene under various conditions at a high level. Further the invention relates to a recombinant DNA fragment comprising the artificial oligonucleotide sequence, an expression plasmid comprising the recombinant DNA fragment and a host cell transformed with the recombinant DNA fragment.
    Type: Grant
    Filed: November 16, 2015
    Date of Patent: September 3, 2019
    Assignee: Clariant International Ltd.
    Inventors: Zdravko Dragovic, Christoph Reisinger, Heiko Dietz
  • Digital counting of individual molecules by stochastic attachment of diverse labels
    Patent number: 10392661
    Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: August 27, 2019
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Stephen P. A. Fodor, Glenn K. Fu
  • CRISPR RNA targeting enzymes and systems and uses thereof
    Patent number: 10392616
    Abstract: The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of a nucleic acid.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: August 27, 2019
    Assignee: Arbor Biotechnologies, Inc.
    Inventors: David R. Cheng, David A. Scott, Winston X. Yan, Shaorong Chong
  • Method for determining clinically relevant hypoxia in cancer
    Patent number: 10385399
    Abstract: The present invention provides a method for determining the oxygen status of a cancer of an individual. The method comprise determining the transcriptional expression level of ADM (SEQ ID No:1), and/or at least one gene selected from ANKRD37 (SEQ ID NO.: 3), P4HA2 (SEQ ID NO.: 12), NDRG1 (SEQ ID NO: 10), SLC2A1 (SEQ ID NO:15), P4HA1 (SEQ ID NO.: 11), LOX (SEQ ID NO.: 9), C3orf28 (SEQ ID NO.: 6), BNIP3L (SEQ ID NO.: 5), BNIP3 (SEQ ID NO.:4), EGLN3 (SEQ ID NO.: 7), PDK1 (SEQ ID NO.: 13), PFKFB3 (SEQ ID NO.: 14), KCTD11 (SEQ ID NO.: 8), and/or ALDOA (SEQ ID NO.: 2), in a cancer sample. The transcriptional level is then correlated to the transcriptional level to at least one reference gene, and oxygen status 10 is then evaluated by comparing the correlated transcription level with a predetermined reference sample comprising cancer cells characterized by a high oxygen level.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: August 20, 2019
    Assignee: AARHUS UNIVERSITET
    Inventors: Jens Overgaard, Brita Singers Sorensen, Jan Alsner, Carsten Wiuf, Marianne Nordsmark, Kasper Toustrup
  • Transcript optimized expression enhancement for high-level production of proteins and protein domains
    Patent number: 10385350
    Abstract: The present invention relates to a system for high-level production of recombinant proteins and protein domains.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: August 20, 2019
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Thomas B. Acton, Stephen Anderson, Yuanpeng Janet Huang, Gaetano Montelione
  • Synthesis of transcripts using Syn5 RNA polymerase
    Patent number: 10378035
    Abstract: Methods of in vitro transcription using cyanophage Syn5 RNA polymerase (RNAP) or mutants thereof and transcription conditions are provided.
    Type: Grant
    Filed: April 19, 2016
    Date of Patent: August 13, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Bin Zhu, Charles C. Richardson, Stanley Tabor
  • Method for preparing recombinant proteins through reduction of rnpA gene expression
    Patent number: 10351890
    Abstract: The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: July 16, 2019
    Assignee: MEDICOSBIOTECH, INC.
    Inventors: Sang Yup Lee, Hannah Chung