Abstract: Process for the production of a protein with citrate lyase activity by expressing a suitable plasmid in a host organism and isolating the protein in an active form, wherein the plasmid contains the information from a gene cluster composed of at least six genes and an inducible promoter. Furthermore the invention concerns the use of the recombinant enzyme and a corresponding test kit for the determination of citric acid.
Type:
Grant
Filed:
September 28, 2000
Date of Patent:
November 2, 2004
Assignee:
Roche Diagnostics GmbH
Inventors:
Michael Bott, Peter Dimroth, Karin Schneider
Abstract: The present invention provides isolated I&kgr;B kinases that regulate NF&kgr;B gene transcription that lack both a leucine zipper like &agr;-helix domain and helix-loop-helix domain. Also provided are the amino acid sequences of these kinases and the nucleotide sequence encoding these kinases, and other related protein and nucleic acid molecules.
Type:
Grant
Filed:
July 3, 2002
Date of Patent:
October 12, 2004
Assignee:
The Research Foundation of State University of New
York
Abstract: The present invention provides methods of producing a pro-N-acetylglucosamine-1-phosphodiester &agr; N-acetyl glucosimanidase (phosphodiester &agr;-GlcNAcase), in mammalian cells deficient in the furin proteolytic enzyme and methods of making lysosomal hydrolases having an N-acetylglucosamine-1-phosphate.
Type:
Grant
Filed:
December 21, 2001
Date of Patent:
October 5, 2004
Assignee:
Genzyme Glycobiology Research Institute, Inc.
Abstract: The present invention relates to a novel D-hydantoinase from Ochrobactrum anthropi that enantio-selectively hydrolyzes D-hydantoins to their corresponding D-N-carbamoyl-amino acids; nucleic acids that encode for the enzyme; expression vectors including the nucleic acids; and host cells capable of expressing the enzyme.
Type:
Grant
Filed:
April 3, 2002
Date of Patent:
October 5, 2004
Assignee:
Bristol-Myers Squibb Company
Inventors:
Michael Politino, Sean M. Tonzi, Guna Romancik, John J. Usher, David A. Lowe
Abstract: The present invention relates to a trehalose-producing microorganism and a process for producing trehalose. It also to a novel trehalose synthase protein, a trehalose synthase gene, recombinant plasmids carrying said trehalose synthase gene, and transformed microorganism with said recombinant plasmids.
Type:
Grant
Filed:
September 24, 2001
Date of Patent:
October 5, 2004
Assignee:
Cheil Jedang Corporation
Inventors:
Se Yong Lee, Eun Kyung Song, Yearn Hung Park, Sang Ho Kwon, Kwang Ho Lee, Chang Gyeom Kim, Jin Ho Lee, Sung Oh Chung, Yeong Joong Jeon
Abstract: The present invention provides amino acid sequences of polypeptides that are encoded by genes within the human genome, the dehydrogenase polypeptides of the present invention. The present invention specifically provides isolated polypeptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the dehydrogenase polypeptides, and methods of identifying modulators of the dehydrogenase polypeptides.
Type:
Grant
Filed:
April 9, 2002
Date of Patent:
September 28, 2004
Assignee:
Applera Corporation
Inventors:
Fangcheng Gong, Chunhua Yan, Valentina Di Francesco, Ellen M. Beasley
Abstract: The present invention relates to recombinant DNA encoding the PpuMI restriction endonuclease as well as PpuMI methylase, expression of PpuMI restriction endonuclease and PpuMI methylase in E. coli cells containing the recombinant DNA.
Abstract: The present invention relates, in general, to a kinase which in its activated state is capable of site-specific phosphorylation of I&kgr;B&agr;, I&kgr;B&agr; kinase. In particular, the present invention relates to the purified kinase, antibodies having binding affinity specifically to the kinase, and hybridomas containing the antibodies. This invention further relates to bioassays using protein or antibodies of this invention to diagnose, assess, or prognose a mammal afflicted with an undesired activation of NF-&kgr;B. This invention also relates to ligands, agonists, and antagonists of the kinase, and diagnostic and therapeutic uses thereof. This invention also relates to bioassays using the kinase to identify ligands, agonists, and antagonists. More specifically, this invention relates to selective inhibitors of the kinase.
Abstract: The invention relates to the use of the N-acetyl amino acid racemase from Amycolatopsis orientalis subspecies lurida for the racemiszation of N-carbamoyl amino acids. This use permits the 100% preparation of optically pure amino acids starting from racemic hydantoins in an enzymatic overall process.
Type:
Grant
Filed:
October 11, 2001
Date of Patent:
July 27, 2004
Assignee:
Degussa AG
Inventors:
Andreas Bommarius, Karlheinz Drauz, Stefan Verseck, Maria-Regina Kula
Abstract: The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.
Type:
Grant
Filed:
September 28, 2001
Date of Patent:
July 20, 2004
Assignee:
New England Biolabs, Inc.
Inventors:
Shuang-yong Xu, Andrew Dore, Adam Hume, John Pelletier, Jing Zhou
Abstract: The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.
Type:
Grant
Filed:
May 24, 2000
Date of Patent:
July 6, 2004
Assignee:
Third Wave Technologies, Inc.
Inventors:
Wu-Po Ma, Victor I. Lyamichev, Michael W. Kaiser, Natalie E. Lyamicheva, Hatim Taysir Allawi, James J. Schaefer, Bruce P. Neri
Abstract: The invention is directed to nucleic acid sequences which encode polypeptides having PhzO activity, namely, the ability to convert phenazine-1-carboxylic acid to a 2-hydroxylated phenazine, and isolated polypeptides having this activity. The invention is also directed to recombinant nucleic acid molecules, vectors, and host cells including the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in bacterial or plant cells to inhibit fungal pathogens.
Type:
Grant
Filed:
September 27, 2001
Date of Patent:
May 18, 2004
Assignees:
The United States of America, as represented by the Secretary
of Agriculture, Washington State University Research Foundation
Inventors:
Linda S. Thomashow, Shannon M. Delaney, Dmitri V. Mavrodi, David M. Weller
Abstract: The invention uses the existence of tissue-specific telopeptide lysyl hydroxylases, enzymes that hydroxylate lysine residues of the telopeptides of collagen, and the discovery that collagen molecules cross-linked by hydroxyallysine (derived from telopeptide hydroxylysine) are less susceptible to degradation/turnover than collagen molecules cross-linked by means of allysine (derived from telopeptide lysine) due to a different intrafibrillar packing of said collagen molecules. Further, the invention contemplates that one of the characteristics of collagen in scars and other fibrotic tissues is an increase in hydroxyallysine derived cross-links (making the collagen less susceptible to proteolytic breakdown), that this increase in hydroxyallysine cross-links is due to increased levels of telopeptide lysyl hydroxylase, and that telopeptide lysyl hydroxylase plays an important role in the excessive accumulation of collagen in fibrosis/scarring.
Type:
Grant
Filed:
November 29, 1999
Date of Patent:
May 11, 2004
Assignee:
Nederlandse Organisatie voor toegepast-natuurwetenschappelijk
Onderzoek TNO
Inventors:
Johannes Maria TeKoppele, Rudolf Antonius Bank
Abstract: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the drug-metabolizing enzyme peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the drug-metabolizing enzyme peptides, and methods of identifying modulators of the drug-metabolizing enzyme peptides.
Type:
Grant
Filed:
June 6, 2002
Date of Patent:
May 4, 2004
Assignee:
Applera Corporation
Inventors:
Karl Guegler, Karen A. Ketchum, Valentina Di Francesco, Ellen M. Beasley
Abstract: The present invention relates to recombinant DNA which encodes the BsaI restriction endonuclease as well as BsaI methylase, expression of BsaI restriction endonuclease and BsaI methylase in E. coli cells containing the recombinant DNA, and purification of BsaI restriction endonuclease to near homogeneity.
Abstract: Genes encoding novel cellulases, and a gene encoding a protein that facilitates the action of such novel cellulases, the novel cellulases and a protein that facilitates the action of such cellulases, and enzyme preparations containing such proteins are described. The native hosts and the culture medium of said hosts containing said novel cellulases are also disclosed. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
Type:
Grant
Filed:
April 30, 1997
Date of Patent:
April 20, 2004
Assignee:
AB Enzymes Oy
Inventors:
Arja Miettinen-Oinonen, John Londesborough, Jari Vehmaanperä, Heli Haakana, Arja Mäntylä, Raija Lanto, Minna Elovainio, Vesa Joutsjoki, Marja Paloheimo, Pirkko Suominen
Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.
Type:
Grant
Filed:
August 3, 2001
Date of Patent:
April 13, 2004
Assignee:
Genencor International, Inc.
Inventors:
Veronique A. Dartois, James A. Hoch, Fernando Valle, Manoj Kumar
Abstract: &agr;-Hydroxy-&ggr;-carboxymuconic acid-&egr;-semialdehyde dehydrogenase, a gene encoding the enzyme, a recombinant vector containing the gene and a transformant carrying the gene, and processes for producing the enzyme and 2-pyrone-dicarboxylic acid by using the transformant.
Use of the transformant can produce &agr;-hydroxy-&ggr;-carboxymuconic acid-&egr;-semialdehyde dehydrogenase in a large amount, thereby permitting industrial production of 2-pyrone-4,6-dicarboxylic acid.
Type:
Grant
Filed:
October 24, 2001
Date of Patent:
April 6, 2004
Assignees:
Cosmo Research Institute, Cosmo Oil Co., Ltd.
Abstract: This invention provides a novel isolated polynucleotide sequence, called ADARP1 (adenosine deaminase related protein 1), that displays significant homology to the adenosine deaminase (ADA) gene. Also provided is the amino acid sequence of the ADARP1 polypeptide encoded by the polynucleotide of the invention. The RNA for this novel gene is found in variety of tissues, with higher levels observed in the heart, testes, and skeletal muscle compared with others tested. Based on amino acid sequence homology, the ADARP1 protein will likely display the catalytic activity characteristic of ADA. This newly found ADARP1 gene and its encoded product may be useful in the treatment of immunodeficiencies, including severe combined immunodeficiency disease (SCID) and other ADA deficiencies, treatment of male reproductive disorders, testicular disorders and musclo-skeletal disorders.
Type:
Grant
Filed:
August 20, 2001
Date of Patent:
March 16, 2004
Assignee:
Bristol-Myers Squibb Company
Inventors:
John N. Feder, Chandra S. Ramanathan, Gabe Mintier
Abstract: An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of detection of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.
Type:
Grant
Filed:
May 18, 2001
Date of Patent:
March 16, 2004
Assignee:
The Regents of the University of California
Inventors:
Michael Karin, Masahiko Hibi, Anning Lin