Abstract: UO2F2 biosensors, and related U-sensing and/or F-sensing genetic molecular components, genetic circuits, compositions, methods and systems are described, which in several embodiments can be used to detect and/or neutralize uranium and in particular bioavailable UO2F2.
Type:
Grant
Filed:
February 4, 2020
Date of Patent:
February 13, 2024
Assignee:
LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
Abstract: Provided are animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. Provided are polypeptides having lysozyme activity, polynucleotides encoding the polypeptides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Grant
Filed:
December 21, 2017
Date of Patent:
February 13, 2024
Assignee:
Novozymes A/S
Inventors:
Mikkel Klausen, Kirk Matthew Schnorr, Soeren Nymand-Grarup, Peter Bjarke Olsen, Marianne Thorup Cohn, Robert Piotr Olinski, Marc Dominique Morant, Ming Li, Ye Liu, Lars Kobbeeroee Skov, Dominique Aubert Skovlund, Han Bucong
Abstract: Strains of yeasts are provided containing the genes for the production of cannabinoids from fatty acids. The enzymes that mediate cannabinoid production are localized to the cytosol, peroxisome or different compartments within the secretory pathway (e.g., endoplasmic reticulum, Golgi, vacuole) to ensure efficient production. The engineered microorganisms produce cannabinoids in a controlled fermentation process.
Type:
Grant
Filed:
October 4, 2021
Date of Patent:
January 30, 2024
Assignee:
PYRONE SYSTEMS, INC.
Inventors:
Alexander Hutagalung, Jose Miguel Laplaza
Abstract: Control processes are provided for enhancing biological processes for converting gaseous substrates to useful end products such as ethanol. A process includes providing a gaseous substrate comprising one or more of CO and CO2 to a bioreactor at a substrate addition rate. Acetogenic bacteria provided to the bioreactor ferment the gaseous substrate in a fermentation broth. The process includes determining a concentration of carboxylic acids and/or carboxylates in the fermentation broth. A control algorithm uses those concentrations to adjust the gaseous substrate addition rate.
Type:
Grant
Filed:
December 15, 2020
Date of Patent:
January 23, 2024
Inventors:
Michael Neubeck, Trevor Stilwell, Nestor Camargo
Abstract: The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.
Type:
Grant
Filed:
January 10, 2023
Date of Patent:
January 23, 2024
Assignee:
University of Florida Research Foundation, Incorporated
Abstract: The present invention relates to a Candida tropicalis cell line, which comprises a mutant gene, having improved tolerance for cytotoxicity of stromal cells, and a method for producing dicarboxylic acid using the Candida tropicalis cell line. The Candida tropicalis cell line for producing dicarboxylic acid developed according to the present invention has improved tolerance for existing stromal toxicity as well as significantly improved efficiency for producing dicarboxylic acid compared to existing cell lines, thus can be used in biological production of dicarboxylic acid and is expected to have high industrial utility.
Type:
Grant
Filed:
December 4, 2019
Date of Patent:
January 16, 2024
Assignee:
Korea University Research and Business Foundation
Inventors:
Kyoung Heon Kim, Thirumalaisamy Babu, Do Hyoung Kim, Jong Hwa Lee, Jung Moo Lee, Ho Chang Lee, Sung Ho Oh, Su Han Kim, Chang Seok Hyun
Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and/or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
Type:
Grant
Filed:
July 22, 2019
Date of Patent:
January 9, 2024
Assignee:
Novozymes A/S
Inventors:
Michael Glenn Catlett, Monica Tassone, Paul Vincent Harris, Robert Lyle Osborne, Ryoko Kataoka, Shiro Fukuyama, Tomoko Matsui
Abstract: This disclosure provides epimerase enzymes useful for commercial scale production of allulose from fructose. The disclosed enzymes (“epimerase variants”) are variants of Burkholderia multivorans CGD1 xylose isomerase engineered to have improved catalytic activity of about 1.5- to 2-fold compared with the parent enzyme.
Type:
Grant
Filed:
August 7, 2019
Date of Patent:
January 2, 2024
Assignee:
ARCHER DANIELS MIDLAND COMPANY
Inventors:
William Schroeder, Padmesh Venkitasubramanian, Sridhar Govindarajan, Mark Welch
Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
Type:
Grant
Filed:
November 2, 2022
Date of Patent:
December 12, 2023
Assignee:
NOVOZYMES A/S
Inventors:
Randy Deinhammer, Suzanne Clark, Mauricio Quiros, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong, Tomoko Matsui, Shinobu Takagi
Abstract: Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.
Type:
Grant
Filed:
April 19, 2021
Date of Patent:
December 5, 2023
Assignees:
Toyota Motor Corporation, Regents of the University of Minnesota
Abstract: Methods and systems for the enhancement of bioproduct process yields. The present invention provides systems and methods for improvement of the carbon conversion efficiency by yeast of an organic substrate such as a fermentable sugar to a fermentation product such as ethanol. Exemplary yield-increasing/growth-inhibitory compounds include tyrosol (TyrOH), 2-phenylethanol (PheOH), and tryptophol (TrpOH). Immobilization of the yeast, such as in a porous bead or pellet, can further increase yield. Exemplary immobilization included immobilization in calcium alginate beads. The system and methods taught herein demonstrate that product yield such as ethanol yield can be improved by adding yeast yield-increasing/growth-inhibitory molecules to reduce cell growth of yeast species, such as S. cerevisiae, suggesting a strategy to improve the yield of ethanol and other yeast fermentation products by manipulating native biological control systems.
Type:
Grant
Filed:
April 26, 2021
Date of Patent:
November 28, 2023
Assignee:
Colorado State University Research Foundation
Abstract: The present invention relates to chimeric endolysin Lys109 which effectively controls Staphylococcus aureus, wherein the endolysin Lys109 of the present invention has a novel amino acid sequence that has not been conventionally studied. Endolysin Lys109 of the present invention can be used as a biological regulator capable of effectively inhibiting a wide range of Staphylococcus aureus and a biofilm produced thereby. In addition, endolysin Lys109 can kill Staphylococcus aureus without regard for resistance to conventional antibiotics, and thus can be widely used for treatment of diseases caused by Staphylococcus aureus infection. Moreover, endolysin Lys109 can also be used to resolve medical problems caused by Staphylococcus aureus which has antibiotic resistance.
Type:
Grant
Filed:
April 21, 2020
Date of Patent:
November 28, 2023
Assignee:
SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION
Abstract: Peroxyhydroxycarboxylic acid forming compositions and methods for forming peroxyhydroxycarboxylic acids, preferably in situ, using the peroxyhydroxycarboxylic acid forming compositions are disclosed. Methods of using the peroxyhydroxycarboxylic acids, including for treating a surface or a target in need of antimicrobial or sanitizing treatment are also disclosed. Particular applications of using odor-free, low volatility peroxyhydroxycarboxylic acid sanitizers for direct food contact are disclosed.
Type:
Grant
Filed:
January 29, 2021
Date of Patent:
November 21, 2023
Assignee:
Ecolab USA Inc.
Inventors:
Junzhong Li, Allison Prideaux, Richard Staub
Abstract: The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof. Further contemplated are cultured recombinant host cells as well as methods of producing fatty amines by employing these host cells.
Abstract: Blends of enzymes of the same enzyme class (same EC number), such as beta-glucuronidase enzyme blends, are provided that exhibit synergistic levels of activity across a range of substrates as compared to the activity levels of each enzyme in the blend individually. The blends also may exhibit a greater effective substrate range, as well as greater effective pH and/or temperature ranges as compared to single enzyme preparations. Predictive methods for determining optimal enzyme blends, methods for preparing enzyme blends, enzyme blend compositions, enzyme blend formulations and methods of using such enzyme blends are provided.
Type:
Grant
Filed:
December 10, 2021
Date of Patent:
November 7, 2023
Assignee:
INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS, INC.
Inventors:
John Tomashek, Caleb Reece Schlachter, Pongkwan Sitasuwan, Lim Andrew Lee
Abstract: The present invention relates to processes for the formation of pyridinedicarboxylic acid (PDCA), in particular, 2,4-pyridinedicarboxylic acid (2,4-PDCA) and 2,5-pyridinedicarboxylic acid (2,5-PDCA), and mono- and diester derivatives thereof, from 3,4-dihydroxybenzoic acid, via a biocatalytic reaction using, for example, a protocatechuate dioxygenase such as protocatechuate 4,5-dioxygenase or protocatechuate 2,3-dioxygenase, and a nitrogen source. The invention also relates to copolymers that comprise the pyridinedicarboxylic acid monomers and derivatives thereof, processes for the formation of the copolymers and uses for the copolymers.
Abstract: The present invention discloses a recombinant modified extracellular chitinase having an amino acid sequence set forth in SEQ ID NO. 4 or SEQ ID NO. 5 prepared by substituting two tyrosine (Y) residues with histidine (H) in the native chitinase of Brevibacillus laterosporus LAK 1210. The modified chitinase represents an advancement as it has improved thermal stability, wider optimum pH range, high efficacy, improved solubility and low toxicity. The present invention also provides compositions and improved methods for producing and purifying the recombinant modified chitinase by chitin affinity chromatography and chitin adsorption affinity chromatography using shrimp shell and crab shell, for large scale production at low cost. The modified chitinase has wide range of applications including prevention, treatment or modulation of phytopathogenic infection in a plant or a plant part.
Type:
Grant
Filed:
September 17, 2021
Date of Patent:
November 7, 2023
Assignee:
Council of Scientific & Industrial Research
Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
Type:
Grant
Filed:
October 7, 2019
Date of Patent:
November 7, 2023
Assignee:
Novozymes A/S
Inventors:
Monica Tassone, James Ron Huffman, Chee-Leong Soong, Jung Yi
Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.
Type:
Grant
Filed:
November 29, 2022
Date of Patent:
November 7, 2023
Assignee:
Cytiva BioProcess R&D AB
Inventors:
Martin Hall, Karolina Busson, Jean-Luc Maloisel, Helena Skoglar