Abstract: A detectable molecule of the formulaA.sup.3 --(--X--R.sup.1 --E--Det.sup.b).sub.mwhere A.sup.3 is A.sup.2 or a polymer, where A.sup.3 has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; --X-- is selected from the group consisting of ##STR1## a C.sub.1 -C.sub.10 branched or unbranched alkyl or aralkyl, which may be substituted by --OH; --Y-- is a direct bond to --E--, or --Y-- is --E--R.sup.2 -- where R.sup.2 is a C.sub.1 -C.sub.10 branched or unbranched alkyl; Z.sub.a is chlorine, bromine or iodine; E is O, NH or an acylic divalent sulfur atom; Det.sup.b is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: ##STR2## or the 4-hydroxy or acyloxy derivative thereof, where R.sup.3 is C.sub.1 -C.sub.4 alkyl or CH.sub.

Abstract: A monoclonal antibody specific to the lymphokine, and its production is disclosed. The novel lymphokine LK2 is a glycoprotein with a molecular weight of 20,000.+-.2,000 daltons; isoelectric point pI, 6.2.+-.0.3; electrophoretic mobility Rf, 0.29.+-.0.02; cytotoxic on L 929 cells and substantially not growth-inhibitive on KB cell. The lymphokine significantly inhibits the growth of malignant human tumors in vivo. The monoclonal antibody may be of IgM or IgG class, and neutralizes specifically the cytotoxic activity of the lymphokine. Combined use of LK 2 with chemotherapeutic agents such as alkylating agents, metabolic antagonists, antioncotic antibiotics and plant alkaloids enhances greatly the antioncotic effect of the chemotherapeutics.

Abstract: Compositions and methods are disclosed for multiple simultaneous assays of different analytes using radioactive labeled antibodies to the analytes, at least one portion of the assay being an immunoradiometric assay in which there is employed a metal isotope label, e.g., .sup.57 Co, attached to an antibody to the analyte through a chelator, e.g., ethylenediaminetetraacetic acid. Multiple simultaneous immunoradiometric assays can be performed by this method, as can multiple simultaneous assays in which one portion of the assay is an immunoradiometric assay and another portion or portions involve one or more other radioassay techniques.

Abstract: New echiboline derivatives with opioid properties which are 6-hydroxy-9-methyl-echiboline derivatives having, in the 10-position, a hydrocarbon substituent of up to 4 carbon atoms--preferably a group R.CH.sub.2 -- (wherein R represents a hydrogen atom or a hydrocarbon group of up to 3 carbons) and especially a methyl, cyclopropyl-methyl or allyl group.These are made by dealkylating corresponding 6-alkoxy-9-methyl-10-substituted echibolines, thereby converting the 6-alkoxy group to a 6-hydroxy group, especially using boron tribromide as dealkylating reagent.Specific examples are 6-hydroxy-9,10-dimethyl-echiboline, 6-hydroxy-9-methyl-10-allyl-echiboline and 6-hydroxy-9-methyl-10-cyclopropylmethyl-echiboline.The compounds may be in free base or salt form, and be incorporated as active ingredient into pharmaceutical compositions.

Abstract: The invention relates to a measuring apparatus for analyzing a gas mixture for at least one gas contained therein in a very low concentration. Such a gas can, for example, be anesthesia gas in ambient room air. The invention is directed to a type of apparatus wherein the gas mixture is split up into conversion products with a heated catalyzer element during measurement and wherein the conversion products are detected by means of a gas sensor which takes up at least one of the conversion products. The measuring apparatus makes possible a quasi-continuous monitoring of the ambient room air for an anesthesia gas with the aid of such sensors. In order to make this possible, a sequence of measuring phases and regeneration phases are cyclicly repeated. The measuring phases are very short in relationship to the response time of the sensor.

Abstract: Incubation media intended for solid-phase immunometric assays and containing lactoferrin are described. Compared with the media used hitherto, these incubation media have the advantage that they prevent, especially in the case where heated sample material is used in the assays, false results on determination of antigens or antibodies.

Abstract: An obstruction across the flow chamber creates a one dimensional convergence of a sheath fluid. A passageway in the construction directs flat cells near to the area of one dimensional convergence in the sheath fluid to provide proper orientation of flat cells at fast rates.

Abstract: A bio-reactor system wherein a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are apart of a loop providing a constant or intermittent flow of fluid medium in a closed loop.

Abstract: A process and intermediates useful for the invrsion of 2-deoxy-2,2-difluororibose analogs to provide 2-deoxy-2,2-difluoroxylose analogs is described.

Abstract: A device is disclosed for conducting an assay. The device comprises a housing, means enclosed in the housing for capturing a first member of a specific binding pair (sbp) in a zone and for allowing liquid to be transported by capillary action away from the zone, first means in the housing for introducing the sample into the device, and second means in the housing other than the first means for introducing a liquid reagent other than the sample into the device. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay are also disclosed.

Abstract: A unique method and apparatus for dispensing tablets having particular application in the clinical analysis of biological samples. A series of containers are advanced past a plurality of processing stations, one of which is a table dispenser in turn. The processing stations are selectively activated by a controller. A sensor is associated with the tablet dispenser and is arranged to detect whether a tablet has actually entered a container. The sensor is activated only when the tablet dispenser is activated. The controller is responsive to the sensor in dependence upon the condition sensed by the sensor.

Abstract: Liquid containers or receptacles are disclosed with cooperating means for rupturing the receptacles to release contained fluid to rain upon test specimens. Structure is disclosed for rupturing receptacles in response to relative motion occurring between receptacles and test specimen support members or in response to punch means formed integrally with a receptacle. A method of molding a liquid receptacle with an integrally formed punch means for rupturing the receptacle is also disclosed.

Abstract: Picogram amounts of DNA can be detected in a sample by the use of high affinity single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

Abstract: The present invention relates to monoclonal antibodies having high affinity to human pancreatic PLA.sub.2, the production thereof, hybridomas producing them and an immunoassay for human pancreatic PLA.sub.2 using them.

Abstract: A reagent for measuring immune complexes fixed with an anti-C3 antibody Facb fragment. By using the reagent, immune complexes existing in the blood serum or body fluid are quantitatively measured.

Abstract: Murine monoclonal antibodies specific to unique antigenic determinants on mammalian terminal deoxynucleotidyl transferases (TdT). The monoclonal antibodies specifically bind to TdT in a wide variety of mammalian cells including human, mouse, rat, rabbit and bovine origin. The monoclonal antibodies are secreted by hybridoma cell lines derived from fusion of murine plasmacytoma cells with splenocytes from mice immunized with TdT from bovine thymus cells. The monoclonal antibodies can detect small numbers of TdT-positive cells from monitoring of TdT-positive leukemias and lymphomas in multple species, including human.

Abstract: Rabbit-mouse hybridomas are cultured immediately after fusion in a suspension medium supplemented with normal rabbit serum. This results in stable hybridomas and provides a source of intact rabbit monoclonal antibody immunoglobulin of defined specificity. A hybridoma which secretes intact rabbit antibody specific for Group A Streptococcus is exemplified. Diagnostic compositions and test kits containing rabbit monoclonal antibodies produced from such hybridomas are described.This application is a continuation of application Ser. No. 07/189,754, filed May 3, 1988, which in turn is a continuation-in-part of application Ser. No.: 046,439, filed May 4, 1987, now abandoned.

Abstract: Thermochemically induced luminescence is generated in a fluorescent labeled organic compound containing a covalently bonded fluorescent label which is a polycyclic aromatic radical having at least three linearly fused benzene rings and capable of being excited to a fluorescent electronic excited state by energy transfer from an energy donor molecule or radical having an electronic excited state, by a process comprising generating an energy donor radical or molecule by a thermochemical reaction in the presence of the fluorescent label. The energy donor radical may be generated by a chemical reaction in the presence of the fluorescent labeled organic compound or the fluorescent labeled compound itself may be additionally labeled with a radical capable of being excited to an electronic excited state by a thermochemical reaction, i.e., by a process initiated by adsorption of thermal energy.

Abstract: Oligonucleotide probes for diagnosing predisposition to rheumatoid arthritis, capable of specifically hybridizing with5'-TACGGGGTTGR.sub.1 GAGAGCTT-3'or3'-AR.sub.2 GCCCCAACR.sub.3 CR.sub.2 CR.sub.2 CGAA-5'wherein A is adenine, C is cytosine, G is guanine, T is thymine, R.sub.1 is GT or TG, R.sub.2 is T or uracil, and R.sub.3 is CA or AC. Or, capable of specifically hybridizing with5'-GGAGCAGAR.sub.2 GCGGGCCGCGG-3'or3'-CCR.sub.2 CGR.sub.2 CR.sub.2 R.sub.3 CGCCCGGCGCC-5'wherein R.sub.1 is A or G, R.sub.2 is T or uracil, and R.sub.3 is R.sub.2 or C.

Abstract: Assay methods and test kits for detection of antibodies having specificities for a variety of determinants in patient samples are disclosed. The assay is especially suitable for use in evaluating patients for their suitability to receive diagnostic and therapeutic immunoconjugates comprising immunoglobulin constituents derived, at least in part, from a non-human source. The assay methods and test kits utilize a target substance (e.g., immunoglobulin) which has substantially the same binding characteristics as a portion of the immunoconjugate proposed for in vivo administration. A plurality of target substance densities are used for assaying each patient specimen to provide an indication of both the level and relative affinity of circulating antibody capable of binding to the immunoconjugate proposed for treatment.