Patents Examined by Christine Nucker
  • Patent number: 5100805
    Abstract: An improved, copolymer-based immunoassay system, method, and apparatus is highly sensitive and effective in accurately measuring extremely low concentrations, such as 10.sup.-6 to 10.sup.-12 grams per milliliter, of biologically active substances, such as monoclonal or polyclonal antibodies and antigens, cancer markers, proteins, bacteria, viruses, therapeutic drugs, drugs of abuse, and food and water contaminants in fluid samples. The system requires no sample preparation, and is reliable, easy to use, sufficiently low in cost to be readily usable in doctors' offices, small labs, and other low-volume facilities, but is also readily adaptable to hospital and high-volume use. The new system, method, and apparatus for agglutination immunoassay systems includes novel and coordinated carrier particle technology, light application and measurement and chemistry, coacting to provide the multiple advantages of simplicity and accuracy in use, and extreme sensitivity in application, in quantifying immunoassays.
    Type: Grant
    Filed: January 26, 1989
    Date of Patent: March 31, 1992
    Assignee: Seradyn, Inc.
    Inventors: Garth E. Ziege, Patricia C. Andrews, Andris Indriksons, Lawrence E. Kay, Jeffrey E. Wright, Daniel A. Maude
  • Patent number: 5098832
    Abstract: The present invention relates to an isolating medium for the identification of the Salmonella bacterium, wherein a polyol metabolizable by Salmonella and a pH indicator reacting to acidification are added to a culture support containing peptones.The present invention also relates to a process for identifying the Salmonella bacterium from the said medium.
    Type: Grant
    Filed: April 19, 1990
    Date of Patent: March 24, 1992
    Assignee: Technogram
    Inventor: Alain Rambach
  • Patent number: 5096809
    Abstract: Disclosed is a method for detecting analytes in samples of whole blood using solid-phase, permeable support assay devices. The whole blood assays require no steps or reagents other than those necessary to carry out the same analysis on plasma or serum using the devices. The color visualization of the analytical field on which the interpretation of the assay depends is not affected by the presence of intact erythrocytes or any degree of hemolysis in the blood sample.
    Type: Grant
    Filed: July 25, 1988
    Date of Patent: March 17, 1992
    Assignee: Pacific Biotech, Inc.
    Inventors: Fon-Chiu M. Chen, Eugene Fan
  • Patent number: 5097020
    Abstract: An immunogenic conjugate which is the reductive amination product of an immunogenic capsular polymer fragment having at least one reducing group and derived from a bacterial capsular polymer of a bacterial pathogen, and a bacterial toxin or toxoid. The invention also relates to methods for the preparation of the conjugates, a vaccine containing the conjugates which elicits effective levels of anti-capsular polymer antibodies in humans. Also disclosed are methods for inducing active immunization against systemic infection in young mammals caused by bacterial pathogens comprising the administration of an immunogenic amount of the abovedescribed conjugate. In a preferred embodiment, the capsular polymer fragment prior to conjugation has at least one aldehyde group at each end of the fragment. The final conjugate made with such capsular polymers has a lattice or network structure, and provides extremely high levels of anti-capsular polymer antibodies in infants.
    Type: Grant
    Filed: October 18, 1989
    Date of Patent: March 17, 1992
    Assignee: The University of Rochester
    Inventors: Porter W. Anderson, Ronald J. Eby
  • Patent number: 5094943
    Abstract: The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution.The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.
    Type: Grant
    Filed: January 19, 1988
    Date of Patent: March 10, 1992
    Assignee: Boehringer Mannheim GmbH
    Inventors: Joachim Siedel, Albert Roder, Joachim Ziegenhorn
  • Patent number: 5095097
    Abstract: The invention relates to magnetic protein conjugates of the formula IM--NH--CO--(CH.sub.2).sub.n --S--P' Iwith n=1-6, preferably with a n=1 or 2 and particularly preferably with n=1, in which M is a dispersible, magnetically reacting material or particle which carries amino groups, and P' is a protein, to a process for the preparation thereof and to the use thereof for the specific removal of cells or soluble antigens, receptors, substrates, cofactors or carbohydrate determinants from aqueous salt solutions or body fluids or as part of a diagnostic method or as a diagnostic aid, preferably for the removal of cells, preferably for bone marrow depletion or for HLA typing.
    Type: Grant
    Filed: June 15, 1990
    Date of Patent: March 10, 1992
    Assignee: Behringwerke Aktiengesellschaft
    Inventors: Peter Hermentin, Reiner Donges, Udo Franssen, Karlheinz Enssle, Heinz-Jurgen Friesen
  • Patent number: 5093081
    Abstract: A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-insoluble macromolecular substrate, and(B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.The above analytical substance further comprises,(C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct a highly sensitive and simple enzyme immunoassay.
    Type: Grant
    Filed: June 21, 1989
    Date of Patent: March 3, 1992
    Assignees: Fuji Photo Film Co., Ltd., Fujirebio Kabushiki Kaisha
    Inventors: Yukio Sudo, Yoshihiro Ashihara, Toshikage Hiraoka, Isao Nishizono, Shigeki Kageyama, Tetsuji Tanimoto
  • Patent number: 5093082
    Abstract: A purified guaiacum resin which is obtained by isolation from a natural guaiacum resin with chromatography using a gel for reversed chromatography as a stationary phase and a polar solvent as a mobile phase. The purified guaiacum resin does not contain substances causing the repellency of a specimen or constituents exhibiting unstable color development when it is used as a body fluid inspection agent and is subjected to color reaction in the presence of a peroxidase and hydrogen peroxide.
    Type: Grant
    Filed: September 9, 1988
    Date of Patent: March 3, 1992
    Assignee: Dai Nippon Insatsu Kabushiki Kaisha
    Inventors: Masanao Watanabe, Kohji Kuroda, Yoshiko Fujita
  • Patent number: 5091364
    Abstract: A process for the preparation of glycoproteins and proteins from cultures of archaebacteria, comprising culturing archaebacteria, isolating glycoprotein and/or protein from the archaebacteria or their cell walls, enzymatically degrading the glycoprotein and/or protein, purifying and isolating the degradation products, and separating those fractions in the individual purification steps based on the biological activity of the fraction. The products increase the body's defenses against infection.
    Type: Grant
    Filed: July 25, 1990
    Date of Patent: February 25, 1992
    Assignee: Bayer Aktiengesellschaft
    Inventors: Jorg Baumgarten, Helmut Brunner, Inge Flesch, Heinz Hildebrand, Norbert Piel, Michael Sperzel
  • Patent number: 5091318
    Abstract: A method for producing a binding assay device composed of antigens on a cellulose nitrate, cellulose nitrate/acetate or similar solid phase is described. The method involves applying to a solid phase a small amount of an allergen composition, or a pretreated allergen composition, containing a certain concentration of allergen and drying the solution. The device is used by contacting a patient test sample to the immobilized allergen and determining whether or not the test sample contains IgE antibodies for the allergen.
    Type: Grant
    Filed: April 13, 1990
    Date of Patent: February 25, 1992
    Assignee: Abbott Laboratories
    Inventors: Mark A. Anawis, Roger E. Lindberg
  • Patent number: 5089393
    Abstract: Ethanolamine in a sample can be assayed by treating the sample with ethanolamine oxidase, thereby to catalyze a reaction-consuming ethanolamine, oxygen and water, and forming glycolaldehyde, ammonia and hydrogen peroxide. The amount of consumed oxygen or the amount of generated ammonia or hydrogen peroxide is then determined, as a measure of the ethanolamine that was originally the sample. The ethanolamine can appear in the sample as such, or can be liberated simultaneously with or prior to the catalysis reaction, from an ethanolamine derivative, e.g. phosphatidyl ethanolamine by the action of phospholipase D. Ethanolamine oxidase can be produced from Bacillus sp. B-0783 FERM-P No. 5798 in a conventional culture medium, preferably by submerged aeration liquid culturation.
    Type: Grant
    Filed: March 31, 1988
    Date of Patent: February 18, 1992
    Assignee: Toyo Jozo Kabushiki Kaisha
    Inventors: Shigeyuki Imamura, Hideo Misaki
  • Patent number: 5087557
    Abstract: Human monoclonal antibodies capable of specifically reacting with an antigenic determinant of LAV/HTLV-III and cell lines producing those monoclonal antibodies are disclosed. The human monoclonal antibodies may be utilized in a method for determining the presence of LAV/HTLV-III in biological samples, or in a method for separating specific antigenic determinants of LAV/HTLV-III from a mixture. Pharmaceutical compositions containing such a human monoclonal antibody, and a method for significantly reducing the infectivity of LAV/HTLV-III in animals using the composition are also disclosed.
    Type: Grant
    Filed: March 19, 1990
    Date of Patent: February 11, 1992
    Assignee: Genetic Systems Corporation
    Inventor: Janela McClure
  • Patent number: 5082768
    Abstract: An attenuator is included in a reagent medium of a luminescent specific-binding assay to suppress undesirable extraneous light. In one such assay, an analyte in a sample is reacted with a specific binding partner attached to a solid surface, forming an immobilized pair at the surface. One member of the immobilized pair is then allowed to react with a specific binding partner previously conjugated with one component of a luminescent reaction system, and the remaining components are provided in the reagent medium. The resulting light emitted in the luminescent reaction is recorded on photographic film or other photodetector as a measure of the presence and quantity of the analyte in the sample.
    Type: Grant
    Filed: December 28, 1990
    Date of Patent: January 21, 1992
    Assignee: Mast Immunosystems, Inc.
    Inventors: John F. Burd, John W. Dyminski, Vincent A. Marinkovich
  • Patent number: 5079342
    Abstract: A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.
    Type: Grant
    Filed: February 11, 1987
    Date of Patent: January 7, 1992
    Assignee: Institut Pasteur
    Inventors: Marc Alizon, Luc Montagnier, Denise Geutard, Francois Clavel, Pierre Sonigo, Mireille Guyader
  • Patent number: 5079351
    Abstract: The presence or absence of a nucleic acid sequence of an isolate of HTLVI and/or HTLVII in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hydridization probe to the amplified product, either free in solution or after immobilization on a solid support.
    Type: Grant
    Filed: November 26, 1986
    Date of Patent: January 7, 1992
    Assignee: Cetus Corporation
    Inventors: John J. Sninsky, Shirley Y. Kwok, Bernard Poiesz
  • Patent number: 5077044
    Abstract: Live vaccines are provided and methods for preparing the live vaccines for protection of a host from a pathogenic microorganism. The vaccines are prepared by introducing at least one modification in a gene involved in at least one, normally at least two, biosynthetic pathways involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step, e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxotrophic mutants require aromatic amino acids, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid or a highly concentrated source of absorbable iron. The auxotrophic mutations have substantially reduced or nonexistent virulence while retaining the desired immunogenicity to initiate the immunogenic response. Various techniques can be employed for providing the desired change.
    Type: Grant
    Filed: March 21, 1988
    Date of Patent: December 31, 1991
    Assignee: The Board of Trustees of the Leland Stanford Jr. University
    Inventor: Bruce A. D. Stocker
  • Patent number: 5077192
    Abstract: A method for the detection of nucleic acid-containing moieties is described which combines affinity capture of the moiety with detection and identification of the moiety's nucleic acid.
    Type: Grant
    Filed: October 25, 1988
    Date of Patent: December 31, 1991
    Assignee: The General Hospital Corporation
    Inventors: Tsanyang Liang, Jack R. Wands
  • Patent number: 5075213
    Abstract: A DNA probe has been isolated which is capable of hybridizing to an oligonucleotide sequence coding for a polypeptide from a major 64 Kilodalton protein of human cytomegalovirus (HCMVgp64). The probe has a sequence of at least seventeen (17) to as many as seven hundred twenty-one 721) nucleotides. The probe may be labelled as by radioactivity. The probe has been used to screen DNA fragments constituting a subgenomic library of human cytomegalovirus DNA to obtain DNA fragments coding for the major late protein of human cytomegalovirus. The DNA fragments coding for the major late protein of human cytomegalovirus (HCMVgp64) may be hybridized to DNA fragments of HCMV DNA from an individual having human cytomegalovirus infection. The viral DNA can be used as whole HCMV DNA or as fragments formed by digesting the human cytomegalovirus DNA with a restriction endonuclease such as one of the restriction endonucleases EcoRI, BamHI, XbaI, HindIII and PrtI.
    Type: Grant
    Filed: June 20, 1990
    Date of Patent: December 24, 1991
    Assignee: City of Hope
    Inventors: Hema Pande, Arthur D. Riggs, John A. Zaia
  • Patent number: 5075211
    Abstract: Novel peptides are provided having substantially the same sequence as immunologically significant fragments of AIDS-related viruses. The polypeptides can be used as reagents in the determination of exposure of a human host to the virus. Of particular interest is the use of polypeptides in screening blood products.
    Type: Grant
    Filed: November 14, 1986
    Date of Patent: December 24, 1991
    Assignee: Genetic Systems Corporation
    Inventors: Wesley L. Cosand, Linda J. Harris, Raymond L. Houghton
  • Patent number: 5073485
    Abstract: An immunoassay method which measures test samples at high concentration without dilution is disclosed. Said immunoassay comprises the steps of: (1) reacting a material (A) to be measured, an immobilized component (B) which binds specifically to the material (A), and a labelled component selected from the group consisting of (i) a labelled component (C) which binds specifically to the material (A) and (ii) a labelled component (A) thereby obtaining an immuno-complex and (2) detecting or measuring the labelling fragment, wherein the reaction of the material (A) with the immobilized (B) or the reaction of the material (A) with the immobilized (B) and the labelled component is carried out at a pH of 2-4.5.
    Type: Grant
    Filed: November 7, 1989
    Date of Patent: December 17, 1991
    Assignee: Sanyo Chemical Industries, Ltd.
    Inventors: Yoshiyuki Amano, Jun-ichiro Kikutake, Masakazu Sugiura