Abstract: A method of determining one or more interaction parameters for the interaction between an analyte and a ligand using a biosensor, which comprises the steps of: A: providing a sensor surface having the ligand immobilized thereto, B: contacting the sensor surface with a control analyte, C: registering the sensor response from binding of the control analyte to binding sites of the ligand, D: determining the control saturation response (RmaxC) for the interaction between the control analyte and the ligand, E: transforming the control saturation response (RmaxC) to an analyte saturation response (RmaxA) using the relative molar response contribution of the analyte and the control analyte.

Abstract: An immunoassay method in which, by using a sensor chip on which a plurality of capture regions which capture a material to be detected by a first capturing body are arranged separated from each other, the material to be detected captured by the first capturing body is individually detected, wherein the plurality of capture regions are formed by using a different type of first capturing body depending on the type of a material to be detected to be captured, the method having: a detection processing order determination step of determining a detection processing order between the capture regions based on information about a detection processing order between the plurality of the capture regions; and a detection processing step of performing a detection processing for each of the capture regions according to the detection processing order between the plurality of the capture regions, and an immunoassay system using the method.

Abstract: One or more aqueous, near infrared emitting, high yield, highly photoluminescent, stable quantum dots conjugated to one or more biomarkers specific moieties. The conjugated quantum dots have an enhanced detection sensitivity and selectivity and may be formed using a novel and efficient method for conjugating one or more biomarker specific moieties to the quantum dots. The invention is further directed to a method for using the conjugated quantum dots for cancer detection in the margin of excised tissue.

Abstract: An optical biomodule for detecting a disease specific biomarker(s), utilizing enhanced fluorescence emission (due to integration of a three-dimensional (3-D) protruded structure (s)) in a fluidic container/zero-mode waveguide, upon chemical binding of a disease specific biomarker(s) with its corresponding disease specific biomarker binder(s) (e.g., an aptamer(s)) is disclosed.

Abstract: A method for sensing an analyte uses tethered particle motion. A functionalized particle [500] has a first state [504] in which the functionalized particle is bound to the surface and a second state [502] in which the functionalized particle is not bound to the surface, where the functionalized particle switches between the first and second states depending on the presence and absence of the analyte, thereby changing motion characteristics of the functionalized particle depending on the presence of the analyte. A spatial coordinate parameter of the functionalized particle is measured by a detector [516], and a processor [518] determines the presence/concentration of the analyte from changes in the measured spatial coordinate parameter.

Abstract: The present disclosure relates to a device for detecting an analyte in a sample, and especially for detecting antigen/antibody reactions, wherein said device comprises a layer composed of a low-density immiscible compound. The disclosure also relates to a method for detecting an analyte in a sample and especially for detecting antigen/antibody reactions in a device comprising a layer composed of a low-density immiscible compound. The disclosure further relates to the uses of such low-density immiscible compound.

Abstract: The present invention pertains to a surface plasmon enhanced fluorescence analysis device and a surface plasmon enhanced fluorescence measurement method which use GC-SPFS and make it possible to detect a substance to be detected with high sensitivity. This surface plasmon enhanced fluorescence measurement device has: a light source for irradiating the diffraction grating of a chip with excited light; a polarizer for removing linearly polarized light from fluorescent light emitted from a fluorescent substance on the diffraction grating; and a photodetector for detecting the linearly polarized light removed by the polarizer.

Abstract: Devices and methods for the detection of antigens are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.

Abstract: A buoyancy enabled separation method for isolation from a sample including a variety of different cells a sparse subset of cells that is differentiated by a plurality of different cell surface markers. Microbubbles conjugated to antibodies are applied sequentially in a container with the previously used microbubbles disrupted prior to applying the next microbubbles conjugated to a different antibody. A system that includes a syringe-like container with a plunger and closeable opening. Further, a method for activating and expanding isolated T cells by applying antigen presenting microbubbbles having a flexible lipid shell mimicking an antigen presenting cell for generating immunological synapses.

Abstract: There is described a process for improving precision in an analytical test for a target analyte in a sample. The process includes prior to analyzing the sample for a target analyte via a biosensor, introducing to an analytical zone defined by the biosensor a fluid comprising an effective amount of the target analyte.

Abstract: During an assay, optical measurements are made at a sensing surface (112), and at least one “homogeneity-image” of the sensing surface (112) is generated. From this image, an “homogeneity-indicator” is determined for at least one region of interest, and the optical measurements are then evaluated in dependence on said indicator. The homogeneity-indicator may for example be a binary value which indicates if an inhomogeneity was detected or not. If an inhomogeneity was detected, all optical measurements may be rejected, only measurements for the involved region of interest may be rejected, or measurements for a selected sub-area of the involved region of interest (ROI) may be rejected.

Abstract: The subject invention pertains to devices and methods of isolating target cells from a population of cells. The devices comprise of microfluidic channels and aptamers and antibodies attached to the inner surface of the microfluidic channels, wherein the aptamers and the antibodies are capable of specific binding to one or more biomolecules present on the surface of the target cell. The methods of the current invention comprise passing the population of cells through the microfluidic channels to facilitate interaction and capture of the target cells by the aptamers and the antibodies attached to the inner surface of the microfluidic channels, washing to microfluidic channels by a washing solution to remove the cells non-specifically bound to the aptamers and the antibodies attached to the inner surface of the microfluidic channels, releasing the captured target cells from the microfluidic channels, and collecting the released target cells.

Abstract: A system and method are provided for utilizing radiometric fluorescence detection to determine a glucose independent concentration value when measuring frequency bands that do not contain the system isosbestic point. Preferably two bands are chosen such that a first band is below the system isosbestic point, and a second band is above the system isosbestic point, and both points are sufficiently far from the frequency endpoints to maximize the signal to noise ratio.

Abstract: A method for screening an anticancer candidate is disclosed. The method includes immobilizing gold nanoparticles onto a substrate; binding a protein involved in carcinogenesis and metastasis to the immobilized gold nanoparticles, recording a spectrum of the protein conjugate, and analyzing the spectrum to obtain reference data; adding a candidate inhibiting the activity of the protein to the protein conjugate, recording a spectrum of the mixture, and analyzing the spectrum to obtain comparative data; and comparing the reference data with the comparative data to determine whether the candidate inhibits the activity of the protein. The method enables screening of a candidate inhibiting the activity of a protein involved in carcinogenesis and metastasis in an accurate and convenient manner.

Abstract: A method for performing a high dynamic range immunoassay includes (a) measuring a first signal from a sandwich immunoassay to detect a target analyte in a fluidic sample in a fluidic channel and a second signal from a competitive immunoassay associated with the target analyte in the same fluidic sample in the fluidic channel, and (b) determining ratio of the first signal to the second signal to provide a measure of concentration of the target analyte in the fluidic sample, wherein the measure is applicable to a high dynamic range of concentrations of the target analyte.

Abstract: The present invention relates to a diagnostic method for the detection of small quantities of amniotic fluid in the vagina. More specifically, the invention relates to the detection of PAMG-1 in the vagina using anti-PAMG-1 antibodies.

Abstract: The present invention relates generally to an assay for detecting and differentiating multiple analytes, if present, in a single fluid sample, including devices and methods therefore.

Abstract: Disclosed is a biosensor, comprising: a sensor part including a capillary chip configured to detect a target material; and a pre-treatment part connected to the sensor part and configured to provide a mixed solution including a substrate to the sensor part, in which the capillary chip includes a capillary, at least one of antibody for antigen-antibody reaction with the target material inside the capillary, and a photo sensor detecting a color of the substrate.

Abstract: The present disclosure generally relates to lateral flow immunoassay systems, devices and methods, for detecting analytes in biological samples. More specifically, the present disclosure relates to synthetic thread based lateral flow immunofluorescent assay systems, devices and methods.

Abstract: The present invention provides for excitable molecules positioned near metallic structures, wherein the metallic structures have a particles size from about 1 nm to 1000 nm and wherein the excitable molecules have fluorescence, phosphorescence or alpha-fluorescence emissions that are altered due to positioning near the metal structures. The emission spectra are distorted on either the blue or red edges in a range from 1 to 10 nm thereby changing the color of emissions. Further, the width of the emission spectrum is modified either by narrowing or broadening depending on the material of the metallic structures and type of excitable molecule.