Abstract: A subtractive immunoassay for detecting the presence or absence of first and second analytes in a sample includes in the following order the steps of: a) conjugating a first detector antibody to the first analyte to tag the first analyte with a first detector tag; b) conjugating a first capture antibody to the first analyte to capture the first analyte to completely deplete the first analyte from the sample; c) conjugating a second detector antibody to the second analyte in the depleted sample to tag the second analyte with a second detector tag; d) conjugating a second capture antibody to the second analyte to capture the second analyte from the sample; e) detecting presence or absence of the first and second detector tags at the site of the first and second capture antibody, respectively, and thereby detecting the presence or absence of the first and second analytes in the sample.

Abstract: The invention relates to a method for quantitatively determining biological analytes in an aqueous solution in the presence of one or more functionalised surfaces, wherein the aqueous solution comprises at least one type of biological analyte and at least one type of fluorescence marker, characterised in that the quantity and/or concentration of the biological analyte or analytes is determined by measuring the fluorescence emission of the unbound fluorescence markers, as well as to a devices for carrying out said method.

Abstract: The present invention generally relates to the field of analysis for example biological analysis. More specifically, the present invention relates to a process of detecting at least one microorganism present in a sample placed in a closed container, said method comprising essentially the following steps: a) Place said sample in contact in the container with at least one culture medium and a support capable of capturing the microorganism(s) to be detected, b) Close the container, c) Place the container under conditions capable of allowing the growth of the microorganism(s), d) Detect, inside said closed container, using detection means, the presence of the microorganism(s) fixed onto the capture support.

Abstract: A method of capturing a Circulating Tumor Cell (CTC) and Circulating Cancer Cells (CSC) from a sample includes introducing a sample into a flow based multichannel device having a cell capture surface and a flow modification surface under conditions that allow a CTC to bind to a cell rolling-inducing agent and a capturing agent disposed on the cell capture surface. The invention also provides for flow based multichannel devices to capture CTCs and CSCs from a sample.

Abstract: An object of the present invention is to improve visibility by reducing background noise in order to accurately detect the signal from a target substance in an immunochromatographic device. The immunochromatographic device comprises a membrane having a detection region, to which an antibody or antigen serving as a capturing substance capable of capturing a target substance is immobilized, for detecting the target substance by using an antigen or antibody labeled with a labeling carrier that is a colored particle to form a complex of the capturing substance-target substance-labeled antigen or antibody in the capturing substance-immobilized detection region on the device, based on color of the labeling carrier, wherein a colorant having a color complementary to the color of the labeling carrier is allowed to be contained in a dry state in a constituent member of the device such that the colorant is developed together with a specimen when the specimen is developed on the device.

Abstract: The invention relates to a method for determining the result of an agglutination reaction and a microplate used in such a method. Agglutinated and non-agglutinated sample material is separated by means of a separation material such as gel material or a bead matrix in a centrifugation step. Whether an agglutination reaction took place or not is determined by comparing the color intensities and/or the gray levels of images of the top side and the bottom side of the respective reaction vessel. Comparing the color intensities and/or the gray levels of the two images makes the automatic determination reliable and stable. Furthermore, the reaction wells are arranged in a two-dimensional array which provides a high throughput.

Abstract: An apparatus for pretreatment of a sample of whole blood in a discrete fluid analyzing instrument comprises automated means for handling and analyzing the sample and means for performing a pretreatment step on the sample or a sub-sample of the sample. The means for pretreatment are used for immobilizing at least one substance or analyte from the sample or sub-sample wherein the substance or analyte is reversibly immobilized. Usually, the apparatus further comprises means for eluting the substance or analyte from the capture means prior to analysis.

Abstract: Provided are methods for detecting a target molecule or particle suspected to be present in a sample, comprising (a) contacting the sample with (i) a fusion molecule comprising a ligand capable of binding to the target molecule or particle and a binding domain, and (ii) a polymer scaffold comprising at least one binding motif to which the binding domain is capable of binding, under conditions that allow the target molecule or particle to bind to the ligand and the binding domain to bind to the binding motif; (b) loading the polymer into a device comprising a pore that separates an interior space of the device into two volumes, and configuring the device to pass the polymer through the pore from one volume to the other volume, wherein the device further comprises a sensor adjacent to the pore configured to identify objects passing through the pore; and (c) determining, with the sensor, whether the fusion molecule or particle bound to the binding motif is bound to the target molecule or particle, thereby detect

Abstract: Disclosed is a station, for testing an analyte in a sample, enabling accurate and quick reaction and analysis of the sample and a reagent in one apparatus. To this end, the present disclosure provides a station, which is for testing a sample by means of inserting a cuvette, having a standby chamber on which a collecting member is placed, a sample chamber, a reagent chamber and a detection unit. The station comprises: a housing which has an input/output part into which a cuvette is inserted; a driving unit which is provided inside the housing, horizontally moves the cuvette, vertically moves a collecting member, reacts a sample in a sample chamber and a reagent in a reagent chamber, and injects a reaction result thereof into a detection unit; and an optical reader which is provided on the horizontal movement path of the cuvette and is for analyzing the reaction result.

Abstract: The present invention relates to detecting cleavage activity of an enzyme. The various aspects of the invention include an enzyme detection device, kit, method and use for detecting or measuring the presence in a test sample of the activity of an enzyme capable of cleaving a substrate. The invention also relates to indicator and binding molecules useful for carrying out the invention. The enzyme substrate contains a hidden binding site which is only revealed upon cleavage by the enzyme.

Abstract: The present invention relates to an analyte detection device comprising: a sample chamber for storing a mixture solution of a sample comprising an analyte and a reactant comprising particles; a detection chamber for storing a detection solution; and a channel placed between the sample chamber and the detection chamber to prevent the mixture solution and the detection solution from being mixed with each other, the analyte detection device characterized by detecting the analyte by moving the particles from the sample chamber to the detection chamber using moving means.

Abstract: This invention relates generally to devices and methods for performing optical and electrochemical assays and, more particularly, to test devices, e.g., cartridges, methods and systems, wherein the test devices have an entry port configured to receive a test sample into a holding chamber; a first conduit having at least one lateral flow test strip; and a displacement device, such as a pneumatic pump, configured to move a portion of said test sample from said holding chamber into said first conduit. The present invention is particularly useful for performing immunoassays and/or electrochemical assays at the point-of-care.

Abstract: Natural and/or synthetic antibodies for specific proteins are adhered to nanoparticles. The nanoparticles are adhered to a substrate and the substrate is exposed to a sample that may contain the specific proteins. The substrates are then tested with surface enhanced Raman scattering techniques and/or localized surface plasmon resonance techniques to quantify the amount of the specific protein in the sample.

Abstract: A lateral flow immunoassay for the detection of anti-drug antibodies against a biological drug includes a membrane having a capture area, a sample application area, a flow path from the sample application area to the capture area, and a conjugate area located in the flow path. The conjugate area has said biological drug detectably labeled and the capture area has said biological drug immobilized thereto.

Abstract: A cassette is disclosed that permits, with an instrument, quantitative analysis to be performed at the moment of sample testing without additional steps to the end user. The cassette includes a calibration strip and a sample strip. The calibration strip contains known quantities of analyte, from which a calibration curve can be created and applied to the analysis of the sample strip. The disclosed cassette can utilize light transmission or light reflectance techniques. The cassette may include a separate wash port for rapid washing of a high background sample. The disclosed cassette may perform diagnostic tests for humans, animals, environmental sample, and/or food samples. In some cases, the disclosed devices and techniques may be used to monitor efficacy of drug therapy and patient compliance with respect to physician-prescribed medication in a point of care setting.

Abstract: Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

Abstract: The purpose of the present invention is to provide a simple measurement tool which can rapidly measure various chemical substances contained in environments or from inside a living body by a simple or inexpensive means/method.

Abstract: The present invention is directed to methods for increasing sensitivities of immunoassays. The invention utilizes an acid elution condition that preferentially elute specifically bound immune complexes over non-specifically bound complexes from a solid phase, and designs immunoassay protocols that improve the ratio of specific binding to non-specific binding and thereby improving assay sensitivity. The protocol determines the signal of the labeled immunocomplexes eluted from a solid phase.

Abstract: Methods and systems are provided for isolating circulating tumor cells from a peripheral blood supply in order to diagnose early stage cancer and/or evaluate tumor status. In one example, a system for capturing circulating tumor cells includes a substrate having a cell-capturing region, the cell-capturing region having a curved, switchback-like shape and including an array of micropillar structures within the curved, switchback-like shape.

Abstract: Methods, kits and devices for detecting antineoplastic drug contamination of a surface are provided according to aspects of the present invention. According to aspects of the invention, methods for detecting antineoplastic drug contamination of a surface include providing a wetting solution compatible with the antineoplastic drug and formulated to promote release of the drug from the surface to be assayed; providing a solid matrix for reversible absorption of the antineoplastic drug; contacting the solid matrix with the wetting solution, generating an assay matrix; contacting the assay matrix and the surface, generating a surface sample; contacting the surface sample with a volume of wetting solution, generating a fluid test sample; and quantifying the antineoplastic drug in the fluid test sample by lateral flow assay to produce an assay result, thereby detecting antineoplastic drug contamination of the surface.