Patents Examined by Christopher Low
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Patent number: 7727739Abstract: The invention relates to fluorescence methods for measuring enzyme activity, in particular enzyme cleaving and joining activities. The invention also relates to fluorogenic substrates which are useful for measuring enzyme activity and as in vitro and in vivo imaging probes.Type: GrantFiled: April 17, 2003Date of Patent: June 1, 2010Assignee: GE Healthcare UK LimitedInventor: John G. Whateley
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Patent number: 7718579Abstract: A method for electrochemical removal of acid-labile protecting groups on an electrode microarray using an organic solution is disclosed. The solution comprises a hydrazine derivative and a salt in an organic solvent. The hydrazine derivative has at least one hydrazine group having at least one hydrogen. The hydrazine derivative provides acidic reagent when an electrode is active and isolates the acidic reagent to the area around the active electrode. The salt is an organic salt or ionic liquid having a concentration sufficient to provide electrochemical conductivity under an applied voltage. During the applied voltage, acidic reagent is generated, which removes acid-labile protecting groups thereby allowing continued addition of monomers to build a custom microarray of oligonucleotides, peptides, or other polymers.Type: GrantFiled: September 13, 2004Date of Patent: May 18, 2010Assignee: CombiMatrix CorporationInventor: Karl Maurer
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Patent number: 7713745Abstract: The invention relates to immobilization methods, in particular for immobilizing indicator molecules on supports such as sensors and to sensors having those molecules immobilized to their surface. Non-covalent immobilization of macromolecular indicator molecules on those supports via mechanical interlacing with polymers at the surface of a support and via ionic bonding via charged moieties of indicator molecules and ionic groups on the surface of the support are disclosed.Type: GrantFiled: April 13, 2004Date of Patent: May 11, 2010Assignee: Sensors for Medicine and Science, Inc.Inventors: Arthur E. Colvin, Jr., Carrie R. Lorenz
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Patent number: 7704924Abstract: A compound of formula (IV): O is a solid support; L is a linking group or a single bond; X? is selected from CO, NH, S, or O; A is O, S, NH, or a single bond; R2 and R3 are independently selected from: H, R, OH, OR, ?O, ?CH—R, ?CH2, CH2—CO2R, CH2—CO2H, CH2—SO2R, O—SO2R, CO2R, COR, CN and there is optionally a double bond between C1 and C2 or C2 and C3; R6, R7, and R9 are independently selected from H, R, OH, OR, halo, nitro, amino, Me3Sn; R11 is either H or R; Q is S, O or NH; R10 is a nitrogen protecting group; and Y is a divalent group such that HY?R, and other related compounds and collections of compounds.Type: GrantFiled: June 24, 2003Date of Patent: April 27, 2010Assignee: Spirogen LimitedInventors: David Edwin Thurston, Philip Wilson Howard
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Patent number: 7700524Abstract: Activated Supports, support-bound activators, strongly acidic supports, and silylating supports are provided; the activated support having the formula (I) wherein L is a linking group component; X is F, CL, OH, and trisubstituted silyloxy; and the shaded circle represents a solid or semi-solid support. Methods of using the activated supports in solid phase organic sync) thesis are also provided.Type: GrantFiled: January 11, 2002Date of Patent: April 20, 2010Assignee: GE Healthcare LimitedInventors: Christopher Holmes, Yijun Pan
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Patent number: 7696130Abstract: Provided is a PNA (Peptide Nucleic Acid) chip in which a probe PNA containing a desired DNA sequence is immobilized on a plastic substrate coated with an epoxy group-containing polymer. Therefore, single-stranded PNAs can be immobilized on a transparent plastic substrate by means of an epoxy group-containing polymer layer in an efficient and cost-effective manner. Fluorescence signal detection based on PNA/DNA hybridization enables identification of SNP (Single Nucleotide Polymorphism).Type: GrantFiled: June 21, 2005Date of Patent: April 13, 2010Assignee: LG Life Sciences, Ltd.Inventors: Jeongmin Ha, Jae Young Jang, In Soo Kim
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Patent number: 7670556Abstract: Disclosed is a method for fabricating 1-dimensional micro-arrays using parallel micro-fluidic channels on chemically-modified metal, carbon, silicon, and/or germanium surfaces; a ?L detection volume method that uses 2-dimensional nucleic acid micro-arrays formed by employing the 1-dimensional DNA micro-arrays in conjunction with a second set of parallel micro-fluidic channels for solution delivery, and the 1-dimensional and 2-dimensional arrays used in the methods. The methodology allows the rapid creation of 1- and 2-dimensional arrays for SPR imaging and fluorescence imaging of DNA-DNA, DNA-RNA, DNA-protein, and protein-protein binding events. The invention enables very small volumes necessary for a variety of bioassay applications to be analyzed by SPR. Target solution volumes as small as 200 pL can be analyzed.Type: GrantFiled: July 10, 2002Date of Patent: March 2, 2010Assignee: Wisconsin Alumni Research FoundationInventors: Robert M. Corn, Hye Jin Lee, Terry T. Goodrich
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Patent number: 7655602Abstract: Disclosed are D-peptides and libraries of D-peptides comprising aromatic D-amino acids. Also disclosed are methods for identifying small D-peptides comprising aromatic D-amino acids that bind to proteins of interest.Type: GrantFiled: July 2, 2003Date of Patent: February 2, 2010Assignee: Bio Science International, Inc.Inventor: Byron E. Anderson
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Patent number: 7645721Abstract: A method for coimmobilizing two or more biomolecules on a substrate in a defined ratio is disclosed. The method uses a copolymer conjugated to a number, N, of different types of oligonucleotides. The copolymer can be adsorbed to the surface of the substrate. N types of oligonucleotides complementary to the copolymer-bound oligonucleotides can be conjugated to N types of biomolecules. The types of the copolymer-bound oligonucleotides can be mixed in a defined ratio then adsorbed to the surface. The biomolecule-bound complementary oligonucletides can be conjugated to the copolymer-oligonucleotides to create a substrate with the biomolecules coimmobilized in a defined ratio. The invention also relates to a substrate prepared by the method of the invention.Type: GrantFiled: February 4, 2002Date of Patent: January 12, 2010Inventors: Karin D. Caldwell, Jennifer A. Neff
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Patent number: 7625844Abstract: The instant invention provides a live labeling microorganism assay for screening a one-bead one-compound library to identify synthetic anti-adherent compounds for blocking biofilm formation. The compounds of libraries may be peptides, small molecules, nucleic acids, other types of molecules or a combination of the foregoing. The live labeling microorganisms are incubated with a one-bead one-compound library for a long period with fresh-labeled bacteria and nutrition replacement in regular intervals. Those beads without bacterial adhesion are transferred into nutritional agar for the culture. The true anti-adherent beads are isolated and the sequences of each isolated compound-bead are determined with sequencer. The anti-adherent compounds identified in this invention are proved to possess long-term effect of blocking bacterial adhesion and biofilm formation.Type: GrantFiled: April 17, 2006Date of Patent: December 1, 2009Assignee: Biopath Laboratory Incorporated (Delaware)Inventors: Fan Yang, Guang Yang
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Patent number: 7595279Abstract: A polyelectrolyte having multiple exposed functional groups, each such group being capable of covalently bonding to a molecule, is immobilized on a surface for the purpose of bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, e.g., an amine functionalized oligonucleotide. The polyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is bound to a functionalized surface using a covalent immobilization strategy, e.g., reaction with the surface of a tosyl-activated microparticle. Following such reaction, exposed reactive functional groups on the protein, such as amine, carboxyl, thiol, hydroxyl groups can further be utilized to covalently couple the oligonucleotide of interest using suitable chemistry.Type: GrantFiled: September 22, 2004Date of Patent: September 29, 2009Assignee: BioArray Solutions Ltd.Inventors: Xinwen Wang, Sukanta Banerjee
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Patent number: 7585929Abstract: The present invention is directed to polyarylates comprising repeating units having the structure: as well as their preparation and use as cell growth substrates.Type: GrantFiled: July 13, 2004Date of Patent: September 8, 2009Assignee: Rutgers, The State University of New JerseyInventors: Ken James, Stephen Brocchini, Varawut Tangpasuthadol, Joachim B. Kohn
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Patent number: 7566765Abstract: The present invention provides heterocyclic compounds having a nine-membered ring of three repeating C—C—N subunits covalently bound to each other through amide bonds, and variable side groups linked to a central C of each subunit. Also described herein are cells containing the cyclic compounds, methods of screening those cells to identify a cyclic compound of interest, and libraries of cyclic compounds and their encoding nucleic acids. The subject compounds may be employed in a variety of research and medical applications, including methods of treating a patient for hepatitis C infection.Type: GrantFiled: January 10, 2005Date of Patent: July 28, 2009Assignee: Rigel Pharmaceuticals, Inc.Inventor: Todd M. Kinsella
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Patent number: 7560417Abstract: Synthesis of chain molecules such as DNA is carried out in a conduit having an interior channel with an inlet end and an outlet end. At least one wall of the conduit is substantially transparent to selected wavelengths of light. Solid carrier particles are contained within the interior channel of the conduit. A plurality of controllable light sources are mounted at spaced locations along the length of the transparent wall of the conduit to allow selective illumination of separated sections of the particles within the conduit. When a light source is turned on, a photodeprotecting group is removed from the carrier particles in the section that is illuminated by the light source. A reagent containing a selected base is flowed through the conduit so that the base will attach to the carrier particles in those sections which have been exposed to light and deprotected.Type: GrantFiled: January 13, 2005Date of Patent: July 14, 2009Assignee: Wisconsin Alumni Research FoundationInventor: Francesco Cerrina
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Patent number: 7550410Abstract: A method and apparatus are provided for performing light-directed reactions in spatially addressable channels within a plurality of channels. One aspect of the invention employs photoactivatable reagents in solutions disposed into spatially addressable flow streams to control the parallel synthesis of molecules immobilized within the channels. The reagents may be photoactivated within a subset of channels at the site of immobilized substrate molecules or at a light-addressable site upstream from the substrate molecules. The method and apparatus of the invention find particularly utility in the synthesis of biopolymer arrays, e.g., oligonucleotides, peptides and carbohydrates, and in the combinatorial synthesis of small molecule arrays for drug discovery.Type: GrantFiled: November 14, 2006Date of Patent: June 23, 2009Inventor: Robert S. Foote
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Patent number: 7541003Abstract: The present invention provides an adsorbent chip, which includes three components, a substrate, an intermediate layer of linker arms and an adsorbent film, which is attached to the linker arms. The adsorbent film is made up of a plurality of adsorbent particles, each of which includes a binding functionality. The invention also provides a method of making the chips of the invention in which the substrate-intermediate film cassette is formed and the adsorbent film is subsequently immobilized thereon. When the adsorbent film is from the same preparation across a particular batch of chips, the chips provide for the acquisition of data that are highly reproducible from one chip to the next throughout the particular batch of chips. Additionally, the invention provides methods for using the chips to perform assays.Type: GrantFiled: July 16, 2002Date of Patent: June 2, 2009Assignee: Bio-Rad Laboratories, Inc.Inventors: Christopher A. Pohl, Steven C. Papanu
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Patent number: 7534563Abstract: Methods for producing ligand substrates and arrays, e.g., peptide and nucleic acid arrays, as well as the arrays produced thereby, methods for use of the arrays and kits that include the same are provided. In the subject methods, a substrate having a surface displaying photocleavable functional groups that produce surface bound photocleavage produced functional groups upon irradiation is first provided, where the photocleavable groups are then cleaved to produce a surface that displays desired functional groups. The resultant substrates, optionally after an additional functionalization step, are then contacted with ligands or monomeric precursors thereof, e.g., via deposition of each different ligand onto a different region of the surface, resulting in covalent attachment of the contacted ligands to the surface.Type: GrantFiled: June 30, 2003Date of Patent: May 19, 2009Assignee: Agilent Technologies, Inc.Inventor: John S. Hargreaves
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Patent number: 7534742Abstract: The present invention relates to enzymatic activity involved in isoprenoid biosynthesis as well as to inhibitors, notably herbicides, for enzymes in the biosynthesis of isoprenoids. More specifically, the present invention relates to screening methods for detecting such inhibitors, and to enzymatically active proteins for performing said methods as well as purified isolated DNA coding for such proteins. Moreover, the present invention relates to novel inhibitors detectable by said screening methods as well as compositions and processes for inhibiting the synthesis of isoprenoids and for controlling the growth of organisms based on said inhibitors. The invention relates also to the development of inhibitor-resistant plant enzymes and plants, plant tissues, plant seeds and plant cells.Type: GrantFiled: September 25, 2006Date of Patent: May 19, 2009Inventors: Wolfgang Eisenreich, Monika Fellermeier, Markus Fischer, Stefan Hecht, Stefan Herz, Klaus Kis, Holger Lüttgen, Felix Rohdich, Silvia Sagner, Christoph A. Schuhr, Jurathip Wungsintaweekul, Adelbert Bacher, Meinhart H. Zenk
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Patent number: 6528652Abstract: Thiazole esters are suitable for detecting the presence of leukocytes in urine. Such thiazole esters are suitable for use in compositions, diagnostic devices, and methods for detecting the presence of leukocytes. A thiazole ester of the invention is of the formula: or a salt or solvated salt thereof, in which A is an N-blocked amino acid residue or N-blocked peptide chain, preferably an alanine residue or polyalanine chain; and R1 and R2 are each independently hydrogen, unsubstituted or substituted aryl, unsubstituted or substituted heteroaryl, unsubstituted or substituted alkyl, unsubstituted or substituted alkenyl, unsubstituted or substituted alkoxy, amino, unsubstituted or substituted acyl, halo, nitro, cyano, —SO3H, or hydroxy, with the proviso that R1 and R2 are not both hydrogen. In one embodiment, at least one of R1 and R2 is methoxy, ethoxy, propoxy, or butoxy. In still another embodiment, R1 is hydrogen and R2 is methoxy, ethoxy, propoxy, or butoxy.Type: GrantFiled: July 30, 1999Date of Patent: March 4, 2003Assignee: ChronimedInventor: Nam-Won Huh
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Patent number: 5183950Abstract: Methods and materials for inducing anhydrobiosis in entomogenous nematode infective juveniles and then maintaining and storing them in an apparently anhydrobiotic state are described. Infective juveniles are induced into an anhydrobiotic state at relatively high relative humidity prior to optional lowering of the ambient relative humidity for storage and shipment. Suitable containers are also disclosed.Type: GrantFiled: September 28, 1987Date of Patent: February 2, 1993Assignee: BiosysInventors: Irene Popiel, Karen D. Holtemann, Itamar Glazer, Christopher Womersley