Patents Examined by Christopher M. Gross
  • Patent number: 10352931
    Abstract: The present invention provides a diagnostic device allowing highly specific and efficient in vivo and/or in vitro detection of a bio marker in a broad range of bodily fluids or tissues. The diagnostic device is composed of a binding agent that specifically binds a bio marker present in the bodily fluid linked by a linker compound or layer to the substrate, which includes a metallic, a semiconductor, or a polymeric carrier. The present invention further provides methods using said device for the detection of bio markers, as well as kits comprising said device and suitable ingredients for the detections of bio markers in a bodily fluid. Furthermore, the invention provides suitable in vivo and in vitro applications of said binding agent for the detection of specific disease-related target structures.
    Type: Grant
    Filed: August 27, 2014
    Date of Patent: July 16, 2019
    Assignee: GILUPI GMBH
    Inventor: Björn Nowack
  • Patent number: 10351847
    Abstract: A carrier to which is attached a peptide and DNA encoding the peptide, wherein the peptide includes at least one non-natural amino acid and/or wherein the peptide has a constrained secondary structure; or a carrier to which is attached ?2 microglobulin, a peptide, and DNA encoding the peptide, said carrier not bearing an MHC or MHC-like molecule.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: July 16, 2019
    Inventors: Graham Ogg, Li-Chieh Huang, Terence Rabbitts
  • Patent number: 10345313
    Abstract: Disclosed are methods for identifying one or more amino acid molecules and nucleic acid molecules encoding such amino acid molecules of at least two proteomes that are conserved, unique or express at higher or lower levels in at least one of the proteomes. Expression libraries are used that produce the proteome, and in one embodiment, may produce the proteome from at least one cDNA expression library in one to five reactions. Anti-proteome antibodies are prepared that selectively bind to one of the proteomes and binding with at least one second proteome compared.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: July 9, 2019
    Assignee: Pathovacs, Incorporated
    Inventor: Manohar John
  • Patent number: 10329558
    Abstract: An object of the present invention is to provide a method of stably introducing a heterocycle into a substrate peptide by using an azoline backbone introducing enzyme. The present invention provides a method of introducing a heterocycle into a leader-sequence-free substrate peptide by using an azoline backbone introducing enzyme to which a leader sequence of the substrate has been bound.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: June 25, 2019
    Assignee: The University of Tokyo
    Inventors: Hiroaki Suga, Yuki Goto, Shotaro Tsunoda
  • Patent number: 10302546
    Abstract: Multiplexing microparticles by measurement of particle length and dye. Utilization of length-based measurements in combination with dye concentrations to attain multiplexing for bioassays.
    Type: Grant
    Filed: April 8, 2015
    Date of Patent: May 28, 2019
    Inventor: Eugene Y. Chan
  • Patent number: 10300451
    Abstract: The present disclosure provides a method for generating an affinity reagent library against a target protein which interacts with a ligand, which comprises the following steps; •i) determining one or more structural element(s) of the ligand which are involved in ligand: target protein interaction; •ii) producing a library of peptides which retain these structural element(s); and •iii) grafting each peptide from the library of peptides into a portion of the affinity reagent molecule such that it may interact with the target protein, in order to produce an affinity reagent library.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: May 28, 2019
    Assignees: Queen Mary University of London, University of Kent
    Inventors: Ahuva Nissim, John Marshall, Mark Howard
  • Patent number: 10301664
    Abstract: Disclosed herewith is drug repurposing efforts that lead to the discovery of prior non-antibiotic drugs can be used in clinical applicable ranges to treat patients of bacterial infection. These repurposed drug can be used either alone or in combination with traditional antibiotic drugs to treat bacterial strains that may develop or already have developed drug resistance.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: May 28, 2019
    Assignee: Purdue Research Foundation
    Inventor: Mohamed Seleem
  • Patent number: 10294519
    Abstract: A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
    Type: Grant
    Filed: May 16, 2012
    Date of Patent: May 21, 2019
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Toshiro Saito, Koshin Hamasaki, Satoshi Takahashi, Muneo Maeshima, Kyoko Imai, Kazumichi Imai, Ryuji Tao
  • Patent number: 10288608
    Abstract: The present disclosure provides polynucleotide conjugates, methods, and assay systems for use in detecting the presence, absence, and/or amount of an analyte in a sample. Various polynucleotide conjugates, conjugate pairs, sets, libraries, and assay systems comprising the same are disclosed. In particular, methods and assay systems for antibody detection and analysis are provided. For example, assays capable of high levels of multiplexing are used for antibody detection and analysis in a biological sample, e.g., Lyme disease patient samples. The presently disclosed polynucleotide conjugates, methods, and assay systems can be used to provide sensitive and reliable diagnosis, even at early stages of a disease or condition. Use for monitoring disease progression and prognosis is also disclosed.
    Type: Grant
    Filed: November 7, 2014
    Date of Patent: May 14, 2019
    Assignee: Prognosys Biosciences, Inc.
    Inventors: Igor A. Kozlov, John Andrew Altin, Petr Capek, Mark S. Chee
  • Patent number: 10287573
    Abstract: DNA taggants in which the nucleotide sequences are defined according to combinatorial mathematical principles. Methods of defining nucleotide sequences of the combinatorial DNA taggants, and using such taggants for authentication and tracking and tracing an object or process are also disclosed.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: May 14, 2019
    Assignee: JEANSEE LLC
    Inventor: Anthony J. Macula
  • Patent number: 10287576
    Abstract: Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: May 14, 2019
    Assignee: NUEVOLUTION A/S
    Inventors: Thomas Franch, Mikkel Dybro Lundorf, Søren Nyboe Jakobsen, Eva Kampmann Olsen, Anne Lee Andersen, Anette Holtmann, Anders Holm Hansen, Anders Malling Sørensen, Anne Goldbech, Daen de Leon, Ditte Kievsmose Kaldor, Frank Abildgaard Sløk, Gitte Nystrup Husemoen, Johannes Dolberg, Kim Birkebæk Jensen, Lene Petersen, Mads Nørregaard-Madsen, Michael Anders Godskesen, Sanne Schrøder Glad, Søren Neve, Thomas Thisted, Tine Titilola Akinleminu Kronborg, Christian Klarner Sams, Jakob Felding, Per-Ola Freskgard, Alex Haahr Gouliaev, Henrik Pedersen
  • Patent number: 10266894
    Abstract: This application discloses methods for cDN?A synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (INA) at the TSO 3? end, using a methyl group donor, and/or a MgCb concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3?-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: April 23, 2019
    Inventors: Rickard Sandberg, Simone Picelli, Omid R. Faridani
  • Patent number: 10261075
    Abstract: There is disclosed a microarray having base cleavable linkers and a process of making the microarray. The microarray has a solid surface with known locations, each having reactive hydroxyl groups. The density of the known locations is greater than approximately 100 locations per square centimeter. Optionally, oligomers are synthesized in situ onto the cleavable linkers and subsequently cleaved using a cleaving base. Optionally, the oligomers are cleaved and recovered as a pool of oligomers.
    Type: Grant
    Filed: May 25, 2018
    Date of Patent: April 16, 2019
    Assignee: CustomArray, Inc.
    Inventors: Karl Maurer, Dominic Suciu, Hetian Gao
  • Patent number: 10234460
    Abstract: An object of the present invention is to provide a peptide excellent in resistance against metabolism, having a stable structure in vivo, and capable of penetrating a cell membrane and reaching in cells. The present invention provides a macrocyclic peptide having a macrocyclic structure comprised of four or more amino acids. At least two amino acids not adjacent to each other have a hydrophobic side chain and the hydrophobic side chains interact with each other inside the ring of the macrocyclic peptide in a hydrophilic environment.
    Type: Grant
    Filed: August 26, 2014
    Date of Patent: March 19, 2019
    Assignee: The University of Tokyo
    Inventor: Hiroaki Suga
  • Patent number: 10227583
    Abstract: High-throughput methods for screening large populations of variant proteins are provided. The methods utilize large-scale arrays of microcapillaries, where each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be isolated, and cells expressing the variant proteins of interest can be characterized. Also provided are systems for performing the disclosed screening methods.
    Type: Grant
    Filed: April 17, 2017
    Date of Patent: March 12, 2019
    Inventors: Jennifer R. Cochran, Bob Chen, Spencer Caleb Alford
  • Patent number: 10221436
    Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.
    Type: Grant
    Filed: January 7, 2016
    Date of Patent: March 5, 2019
    Assignee: 10X GENOMICS, INC.
    Inventors: Paul Hardenbol, Pranav Patel, Benjamin Hindson, Paul William Wyatt, Keith Bjornson, Indira Wu, Kamila Belhocine
  • Patent number: 10221444
    Abstract: Disclosed are methods and compositions for preparing a DNA library from slide-mounted FFPE tissue samples for downstream next-generation sequencing. In one embodiment, a method of preparing the DNA library is disclosed. The method can comprise applying a droplet of a reagent mixture onto a slide-mounted FFPE tissue sample. The reagent mixture can comprise one or more buffer solutions, a cofactor, a nonionic surfactant, a glycerol solution, a gelatin solution, dNTPs, a DNA polymerase, and a primer pool comprising a plurality of forward primers and reverse primers. The method can also comprise stirring the droplet in a circular motion on the slide while scraping portions of the FFPE tissue sample mounted on the slide to yield a reaction mixture. The method can further comprise aspirating the reaction mixture from the slide directly into a pipette tip and dispensing the reaction mixture into a reaction vessel for further amplification.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: March 5, 2019
    Assignee: URIT Medical Electronic Co., Ltd.
    Inventor: Tom Cheng Xu
  • Patent number: 10197567
    Abstract: An object of the present invention is to provide a method of efficiently constructing a library abundant in diversity and also usable for screening of a compound that binds to a target substance having protease activity. The present invention provides a method of constructing an azoline compound library containing two or more azoline compounds having an azoline backbone introduced into at least one of Cys, Ser, Thr, and 2,3-diamino acid, and analogs thereof of Xaa0 of a peptide represented by the following formula (I): A-(Xaa0)n-B??(I) [wherein, m numbers of Xaa0s respectively represent arbitrary amino acids, at least one of which is an amino acid selected from the group consisting of Cys, Ser, Thr, and 2,3-diamino acid, and analogs thereof, m represents an inter selected from 2 to 40, and A and B each independently represent a peptide composed of from 0 to 100 amino acids].
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: February 5, 2019
    Assignee: The University of Tokyo
    Inventors: Hiroaki Suga, Yuki Goto, Yumi Ito
  • Patent number: 10195580
    Abstract: A method of creating a mask on a surface of a substrate is disclosed. The substrate comprises a plurality of spaced heating elements on or proximal to the surface. The method comprises applying a layer of masking material to the surface and employing the heating elements to apply energy to a phase change in the masking material at the selected sites such that it adheres to the surface or can be displaced from the surface to mask or unmask the selected sites respectively. A method of synthesising an array of molecules, an apparatus for selectively masking one or more sites on a surface and a semi-conductor chip that uses micro-heaters to modulate a masking layer on areas of the chip surface.
    Type: Grant
    Filed: August 15, 2014
    Date of Patent: February 5, 2019
    Assignee: EVONETIX LTD.
    Inventors: Daniel Arthur Hall, Christopher John Favero Jordan
  • Patent number: 10197575
    Abstract: The current invention pertains to stabilized peptoids or peptoid-peptide hybrids. The peptoids or peptoid-peptide hybrids are stabilized by side chain-side to side chain linkages and/or backbone cyclization. The current invention also provides a positional library scanning method for identification of peptoids or peptoid-peptide hybrids having a desired biological activity.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: February 5, 2019
    Inventors: Mark McLaughlin, Amod A. Sarnaik