Abstract: A method of determining a number of a solution constituent includes introducing a first number of solution constituents to a first test location, establishing a first binding environment for the introduced first number of solution constituents, creating a first residual number of solution constituents by binding a first plurality of solution constituents, establishing a second binding environment for the first residual number of solution constituents, creating a second residual number of solution constituents by binding a second plurality of solution constituents from the first residual number of solution constituents, obtaining a first signal associated with the bound first plurality of solution constituents, obtaining a second signal associated with the bound second plurality of solution constituents, and determining a second number of a constituent of interest based upon the obtained first signal and the obtained second signal.
Type:
Grant
Filed:
January 15, 2010
Date of Patent:
March 15, 2022
Assignees:
Stanford University, Robert Bosch GmbH
Inventors:
Sam Kavusi, Daniel Roser, Christoph Lang, AmirAli Haj Hossein Talasaz
Abstract: Compositions comprising activated topoisomerase adaptors (TOPO-adaptors) and methods of using the activated TOPO-adaptors are provided for preparing a library of target DNA duplexes derived from sample polynucleotides (e.g., DNA, RNA) for the streamlined preparation of a large number of samples. Such libraries may be used for Next Generation Sequencing (NGS).
Abstract: The present application relates to a system for designing promoters for selective expression of genes. Thereby identified transcription regulatory elements are selected according to a specific methodology and used to create a library of transcription regulatory elements, which are then used to construct specific promoters, especially tissue-specific promoters.
Abstract: A nucleic acid probe, a method of immobilizing the nucleic acid probe to a solid support and the solid support including the immobilized probes using UV light. The nucleic acid probe includes a terminus anchor chain portion, and a capture portion wherein the terminus anchor chain portion includes a sequence of at least 18 nucleotides composed of stretches of up to 5 nucleotides of base type X with intermediate nucleotide(s) of base type Cytosine (C) and optionally one nucleotide of base type Guanine (G) or a sequence with at least 90% similarity thereto, wherein each base type X independently of each other designate base type Thymine (T) or base type Uracil (U).
Type:
Grant
Filed:
August 24, 2018
Date of Patent:
February 15, 2022
Assignee:
ZOETIS SERVICES LLC
Inventors:
Johan Eriksen, Wai Hoe Chin, Martin Jensen Søe, Martin Heller
Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.
Abstract: The present invention includes a multivalent glycan microarray for detection of glycan-binding proteins. The multivalent glycan microarray allows a multivalent presentation of glycan on a microarray substrate, which can enhance binding of the glycan binding protein to the glycan microarray. The multivalent microarray includes a solid substrate having one or more branched polymers bonded to it via one or more silane-based linker reagents. The branched polymer in turn is bonded to a glycan, via one or more bifunctional linkers to form the multivalent glycan microarray. Nonspecific binding of glycan binding proteins to the multivalent glycan microarray can be reduced by using a blocking reagent coated on to the microarray substrate, which includes a polyethylene glycol surfactant attached to the solid substrate via a self-crosslinking azido-functionalized silane. Methods for making multivalent glycan microarrays and methods for using same to detect glycan-binding proteins are also disclosed.
Abstract: The present invention is directed to a method for the synthesis of a bi-functional complex comprising a molecule part and an identifier oligonucleotide part identifying the molecule part. A part of the synthesis method according to the present invention is preferably conducted in one or more organic solvents when a nascent bi-functional complex comprising an optionally protected tag or oligonucleotide identifier is linked to a solid support, and another part of the synthesis method is preferably conducted under conditions suitable for enzymatic addition of an oligonucleotide tag to a nascent bi-functional complex in solution.
Type:
Grant
Filed:
April 16, 2011
Date of Patent:
January 18, 2022
Assignee:
NUEVOLUTION A/S
Inventors:
Alex Haahr Gouliaev, Thomas Franch, Michael Anders Godskesen, Kim Birkebæk Jensen
Abstract: Provided are populations of polypeptides, wherein each member of the population of polypeptides includes or is an amino sequence as set forth in SEQ ID NO: 5 or SEQ ID NO: 6. Also provided are methods for identifying polypeptides that bind to pre-selected target molecules, which in some embodiments can include providing a population of polypeptides as described herein, contacting the population of polypeptides with a pre-selected target molecule, and identifying a complex comprising at least one member of the population of polypeptides bound to the pre-selected target molecule; and populations of nucleic acid molecules that encode the presently disclosed populations of polypeptides.
Type:
Grant
Filed:
November 12, 2019
Date of Patent:
December 28, 2021
Assignee:
Navigo Proteins GmbH
Inventors:
Erik Fiedler, Ulrich Haupts, Madlen Zwarg
Abstract: Techniques regarding measuring a position and transcriptome of one or more cells from a biological sample are provided. For example, one or more embodiments described herein can comprise a method, which can comprise covalently bonding a probe to a molecular structure located on a cell to label the cell according to a position of the cell with regards to a biological sample comprising the cell. The probe comprises an oligonucleotide sequence that can be indicative of the position.
Type:
Grant
Filed:
November 16, 2018
Date of Patent:
December 28, 2021
Assignee:
INTERNATIONAL BUSINESS MACHINES CORPORATION
Inventors:
Robert Vogel, Navneet Dogra, Lena Voith von Voithenberg, Aditya Kashyap, Govind V. Kaigala, Gustavo Alejandro Stolovitzky
Abstract: The invention provides methods, kits, compositions, and devices useful for detection of antibodies that bind to Ehrlichia antigens and/or for differentiation of certain Ehrlichia species from others. In particular, the invention provides methods and kits useful for identifying species of Ehrlichia using populations of isolated peptides.
Type:
Grant
Filed:
November 14, 2017
Date of Patent:
December 21, 2021
Assignee:
ZOETIS SERVICES LLC
Inventors:
Rajesh K. Mehra, Jeremy D. Walker, Kenneth P. Aron, Dennis M. Bleile, Cristina R. Cuesico, Timothy P. Forsyth
Abstract: Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.
Abstract: The invention relates to methods for preparing a compound comprising a peptide attached to a molecular scaffold whereby multiple peptide loops are formed, to compounds that can be obtained with such methods and uses thereof.
Type:
Grant
Filed:
December 6, 2017
Date of Patent:
December 7, 2021
Assignees:
Stichting voor de Technische Wetenschappen, Universiteit van Amsterdam
Inventors:
Gaston Julia Johannes Richelle, Dieuwertje Emma Streefkerk, Jan Herman van Maarseveen, Peter Timmerman
Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
Type:
Grant
Filed:
April 14, 2020
Date of Patent:
November 30, 2021
Assignee:
Pacific Biosciences of California, Inc.
Inventors:
David R. Rank, Jeffery Wegener, Jonas Korlach, Daniel Roitman, Yue Xu, John Lyle, Stephen Turner, Paul Peluso, Geoff Otto, Ron L. Cicero
Abstract: Compositions and methods, systems, and kits for detecting and quantifying variations in numbers of molecules, particularly variations in gene dosage, e.g., due to gene duplication, or to variations from the normal euploid complement of chromosomes, e.g., trisomy of one or more chromosomes that are normally found in diploid pairs, without digital sequencing.
Type:
Grant
Filed:
January 15, 2021
Date of Patent:
November 30, 2021
Assignee:
Progenity, Inc.
Inventors:
Matthew Sekedat, Jeffrey Buis, Ronald David Beaubien, Jr., Sharat Singh, Jeff Perry, Steven Lockton, Chandni Jain, Natalie Mae Jameson-Kiesling
Abstract: The invention, depending on aspect and embodiment, relates to capture probe controls, and capture and signal probe configurations and combinations of configurations that can facilitate accurate and efficient multiplex analyte detection, especially in electrochemical detection schemes.
Type:
Grant
Filed:
April 28, 2020
Date of Patent:
October 26, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Michael Leonard Wood, Aviva Ruth Jacobs
Abstract: Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.
Abstract: The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.
Type:
Grant
Filed:
November 6, 2020
Date of Patent:
October 5, 2021
Assignee:
University of Washington
Inventors:
David Baker, David Younger, Eric Klavins
Abstract: Compositions and methods are provided for the identification of peptide sequences that are ligands for a T cell receptor (TCR) of interest, in a given MHC context.
Type:
Grant
Filed:
September 3, 2020
Date of Patent:
September 21, 2021
Assignees:
The Board of Trustees of the Leland Stanford Junior University, CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventors:
Michael Edward Birnbaum, Juan Luis Mendoza, Michael Thomas Bethune, David Baltimore, Kenan Christopher Garcia
Abstract: Compositions and methods are provided for the identification of peptide sequences that are ligands for a T cell receptor (TCR) of interest, in a given MHC context.
Type:
Grant
Filed:
September 3, 2020
Date of Patent:
September 21, 2021
Assignees:
CALIFORNIA INSTITUTE OF TECHNOLOGY, THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Inventors:
Michael Edward Birnbaum, Juan Luis Mendoza, Michael Thomas Bethune, David Baltimore, Kenan Christopher Garcia
Abstract: The present disclosure provides for compositions of ?PNA probes. Additionally, the present disclosure provide for methods and kits using ?PNA probes for the diagnosis of sepsis.