Patents Examined by Christopher M. Gross
  • Method for synthesising templated molecules
    Patent number: 10731151
    Abstract: The invention relates to a method for synthesising templated molecules attached to the templated which directed the synthesis thereof. The method involves a template, a scaffold functional entity and a functional entity attached to a building block, which, in turn, is attached the template. The scaffold functional entity and the functional entity of the building block are both provided with complementary dimerization domains allowing the functional entities to come into close proximity when the complementary domains interact with to each other. The method may be used for generating libraries of templated molecules which may be selected for biological activity.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: August 4, 2020
    Assignee: NUEVOLUTION A/S
    Inventors: Henrik Pedersen, Anette Holtmann, Thomas Franch, Alex Haahr Gouliaev, Jakob Felding
  • Method for producing peptide library, peptide library, and screening method
    Patent number: 10711268
    Abstract: An object of the present invention is to provide a method for producing a peptide library capable of incorporating an arbitrary number of arbitrary proteinogenic and/or non-proteinogenic amino acids in an arbitrary site. The invention provides a method for producing a peptide library including 1×106 or more kinds of peptides containing amino acids encoded by N1N2N3, including a step of preparing an mRNA library including mRNAs which encode peptides of the peptide library and each contain at least one N1N2N3; and a step of translating each mRNA of the mRNA library in a cell-free translation system added with tRNA containing an anticodon to any one of N1N2N3 codons and charged with an amino acid corresponding to the codon (wherein, N1, N2, and N3 are each independently selected from adenine (A), guanine (G), cytosine (C), and uracil (U) and an arbitrary amino acid is reassigned to each N1N2N3).
    Type: Grant
    Filed: May 12, 2014
    Date of Patent: July 14, 2020
    Assignees: The University of Tokyo, PeptiDream Inc.
    Inventors: Hiroshi Murakami, Takashi Kawakami, Patrick Reid, Toru Sasaki
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10711374
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: July 14, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Size selection of RNA using poly(A) polymerase
    Patent number: 10696994
    Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise adding a polyA tail to the longer RNA fragments but not the shorter RNA fragments in a sample by incubating the population of RNA fragments with a polyA polymerase, wherein the reaction conditions used preferentially tail only the longer fragments but not the shorter fragments.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: June 30, 2020
    Assignee: BIOO SCIENTIFIC CORPORATION
    Inventor: Kevin Allen
  • Selective optimization of a ribosome binding site for protein production
    Patent number: 10696963
    Abstract: A method for optimising a nucleotide sequence for protein expression in a host cell, comprising the steps of: (a) constructing an expression library comprising a large number of variants of the sequence to be expressed operatively cloned in an expression vector, wherein (i) the sequence of the 6 nucleotides immediately upstream (5?-direction) of the first codon of the coding sequence to be expressed is completely or partially randomized; (ii) the sequence of the second and third codons of the coding sequence to be expressed is randomized, wherein the randomization of the second and third codons is limited to changes not altering the amino-acids encoded by said codons; (b) screening the library with regard to efficiency of protein expression in the host cell; and (c) selecting a sequence resulting in a desired level of efficiency of protein expression.
    Type: Grant
    Filed: December 15, 2015
    Date of Patent: June 30, 2020
    Assignee: CloneOpt AB
    Inventors: Daniel Daley, Kiavash Mirzadeh, Stephen Toddo, Suchithra Guntur
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10676842
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: October 31, 2019
    Date of Patent: June 9, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Capture ligand controls, blocking probes, masking probes and methods of using the same
    Patent number: 10670591
    Abstract: The invention, depending on aspect and embodiment, relates to capture probe controls, and capture and signal probe configurations and combinations of configurations that can facilitate accurate and efficient multiplex analyte detection, especially in electrochemical detection schemes.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: June 2, 2020
    Assignee: GenMark Diagnostics, Inc.
    Inventors: Michael Leonard Wood, Aviva Ruth Jacobs
  • Articles having localized molecules disposed thereon and methods of producing same
    Patent number: 10655172
    Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: May 19, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: David R. Rank, Jeffrey Wegener, Jonas Korlach, Daniel Roitman, Yue Xu, John Lyle, Stephen Turner, Paul Peluso, Geoff Otto, Ronald L. Cicero
  • Polypeptide library
    Patent number: 10655125
    Abstract: The invention relates to novel polypeptide libraries that are conformationally constrained in an anti-parallel, helix-turn-helix arrangement. The invention further relates to methods of generating and screening such libraries for biological, pharmaceutical and other uses.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: May 19, 2020
    Assignee: MOR0059US.NP
    Inventors: Roger Müller, Andreas Bültmann, Josef Prassler, Markus Moosmeier
  • Methods for sequencing nucleic acids
    Patent number: 10597715
    Abstract: Provided herein are methods and compositions for the sequencing of long nucleic acids, such as DNA. The methods and compositions are suited for the spatial labeling and sequencing of long nucleic acid molecules.
    Type: Grant
    Filed: December 5, 2014
    Date of Patent: March 24, 2020
    Assignee: Centrillion Technology Holdings
    Inventors: Wei Zhou, Filip Crnogorac, Glenn McGall, Jian Cao
  • Method of drug design and optimisation utilizing stereochemical mimicry
    Patent number: 10591461
    Abstract: The invention relates to a method of designing or optimizing a drug candidate by making stereodynamic derivatives as well as novel derivatives of Glyx-13 with improved biological and pharmacological properties.
    Type: Grant
    Filed: April 27, 2017
    Date of Patent: March 17, 2020
    Assignee: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventor: David M. Chenoweth
  • Peptide array quality control
    Patent number: 10578623
    Abstract: The present application provides arrays for use in immunosignaturing and quality control of such arrays. Also disclosed are peptide arrays and uses thereof for diagnostics, therapeutics and research.
    Type: Grant
    Filed: May 29, 2018
    Date of Patent: March 3, 2020
    Assignee: ARIZONA BOARD OF REGENTS OF BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Neal Woodbury, Stephen Johnston, Phillip Stafford
  • Method for detecting, capturing and/or releasing chemical elements
    Patent number: 10571449
    Abstract: The invention relates to a method suitable for detecting, capturing and/or selectively releasing chemical elements selected from poor metals, alkalines, alkaline earths, actinides and rare earths. Said method involves a molecular assembly formed by at least one amine, and at least one aldehyde and/or an imine and/or CO2, or an adduct formed by the contact between an amine and CO2, and at least one of said chemical elements. The invention also relates to a kit for implementing said method for detection, capture and/or release.
    Type: Grant
    Filed: May 20, 2014
    Date of Patent: February 25, 2020
    Assignees: UNIVERSITE D'AIX-MARSEILLE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, ECOLE CENTRALE DE MARSEILLE, UNIVERSITE CLAUDE BERNARD LYON 1 (UCBL)
    Inventors: Julien Leclaire, Gabriel Canard, Frédéric Fotiadu, Guillaume Poisson
  • Express humanization of antibodies
    Patent number: 10562981
    Abstract: The disclosure provides a method for generation of humanized full length antibodies in mammalian cells. A library of humanized variants is provided with high, validated human framework diversity without requiring back-mutations to retain original affinity. Synthetic CDR encoding fragment libraries derived from a template antibody are ligated to human framework region encoding fragments from a human framework pool limited only to germline sequences from a functionally expressed antibodies. The vector comprises a nucleic acid sequence encoding HC framework region 4. No CDR grafting or phage display is required.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: February 18, 2020
    Assignee: BioAtla, LLC
    Inventor: Jay M. Short
  • Nanopatterned extracellular matrices enable cell-based assays with a mass spectrometric readout
    Patent number: 10563244
    Abstract: The present disclosure provides methods in which adherent cells are treated with small molecules, cultured, lysed, and then analyzed by mass spectrometry to measure the activities of endogenous enzymes. The implementation of this method relies on the use of surfaces that are nanopatterned with cell adhesion ligands to mediate cell attachment and a peptide that is a substrate for the desired enzyme activity in the lysate.
    Type: Grant
    Filed: September 20, 2017
    Date of Patent: February 18, 2020
    Assignee: NORTHWESTERN UNIVERSITY
    Inventors: Milan Mrksich, Maria D. Cebezas, Chad A. Mirkin
  • Nanoscale apertures having islands of functionality
    Patent number: 10562766
    Abstract: Methods, compositions and arrays for non-random loading of single analyte molecules into array structures are provided. Arrays of confined regions are produced wherein each confined region comprises a single island within the confined region. The island can be selectively functionalized with a coupling agent to couple a single molecule of interest within the confined region.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: February 18, 2020
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Stephen Turner, Ron Kuse, Gregory Kearns, Pezhman Monadgemi, Mathieu Foquet, Drew Martinez
  • Stabilized peptoid-peptide hybrids and uses thereof
    Patent number: 10556928
    Abstract: The current invention pertains to stabilized peptoids or peptoid-peptide hybrids. The peptoids or peptoid-peptide hybrids are stabilized by side chain-side to side chain linkages and/or backbone cyclization. The current invention also provides a positional library scanning method for identification of peptoids or peptoid-peptide hybrids having a desired biological activity.
    Type: Grant
    Filed: October 11, 2018
    Date of Patent: February 11, 2020
    Assignees: H. LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE, INC., UNIVERSITY OF SOUTH FLORIDA
    Inventors: Mark McLaughlin, Amod A. Sarnaik
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10557216
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: February 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
    Patent number: 10557158
    Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: February 11, 2020
    Assignee: 10X GENOMICS, INC.
    Inventors: Paul Hardenbol, Pranav Patel, Benjamin Hindson, Paul William Wyatt, Keith Bjornson, Indira Wu, Kamila Belhocine
  • Directed evolution of multivalent glycopeptides that tightly bind to target proteins
    Patent number: 10544412
    Abstract: The invention relates to a method for selecting a glycopolypeptide that binds to a target protein, the method including the steps of providing a pool of glycopolypeptides fused via puromycin linker to an encoding mRNA-cDNA duplex; combining the pool with a target protein to form a mixture; incubating the mixture for a period of time sufficient to allow any target protein to bind to one or more of the glycopolypeptides, thereby forming glycopolypeptide-target protein complexes; and isolating from the mixture the glycopolypeptide-target protein complexes, thereby identifying a plurality of selected glycopolypeptides.
    Type: Grant
    Filed: December 2, 2014
    Date of Patent: January 28, 2020
    Assignees: Brandeis University, The General Hospital Corporation
    Inventors: Isaac J. Krauss, Satoru Horiya, Yollete V. Guillen Schlippe