Patents Examined by Cynthia B. Wilder
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Patent number: 12123052Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.Type: GrantFiled: December 22, 2020Date of Patent: October 22, 2024Assignee: CepheidInventors: Joseph H. Smith, David H. Persing, Alan Wortman, Ronald Chang, David Swenson
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Patent number: 12116624Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.Type: GrantFiled: December 11, 2023Date of Patent: October 15, 2024Assignee: Guardant Health, Inc.Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
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Patent number: 12116640Abstract: Disclosed herein are methods, compositions, and devices for use in the early detection of cancer. The methods include preparing cell-free nucleic acid molecules from a subject for sequencing, sequencing a panel of regions in the cell-free nucleic acid molecules, and detecting one or more markers that are indicative of a cancer.Type: GrantFiled: February 8, 2024Date of Patent: October 15, 2024Assignee: Guardant Health, Inc.Inventors: Stefanie Ann Ward Mortimer, AmirAli Talasaz, Darya Chudova, Helmy Eltoukhy
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Patent number: 12099057Abstract: Methods and systems are directed to multiplex detection of a bacterial pathogen in a sample. A first biotinylated lysin-derived cell wall binding domain is complexed with an avidin layer on a surface. A first bacterial pathogen detection complex including a second biotinylated lysin-derived cell wall binding domain, a detection domain, and an avidin linker complexed between the cell wall binding domain and the detection domain is also provided. The cell wall binding domains are derived from an endolysin, autolysin, bacteriocin, or exolysin, and are configured to bind a cell wall of a target bacterial pathogen. The detection domain includes one or more enzymes, fluorescent material, or DNA for emitting a signal for detection. Target bacterial pathogens present in a sample can thus be detected in a sandwich assay exhibiting increased selectivity and reduced limit of detection relative to traditional ELISA.Type: GrantFiled: May 8, 2018Date of Patent: September 24, 2024Assignee: Rensselaer Polytechnic InstituteInventors: Seok-Joon Kwon, Jonathan Seth Dordick, Domyoung Kim, Jungbae Kim, Inseon Lee
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Patent number: 12084724Abstract: The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.Type: GrantFiled: October 5, 2021Date of Patent: September 10, 2024Assignee: INIVATA LTD.Inventors: Samuel Woodhouse, Stefanie Lensing, Tim Forshew, Vincent Plagnol, Matthew Edward Smith, Karen Howarth, Michael Epstein
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Patent number: 11987847Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.Type: GrantFiled: December 14, 2020Date of Patent: May 21, 2024Assignees: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, EXACT SCIENCES CORPORATIONInventors: David A. Ahlquist, William R. Taylor, Douglas W. Mahoney, Graham P. Lidgard, Hatim T. Allawi
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Patent number: 11970747Abstract: Disclosed in the present invention are a primer-probe combination and a kit for vaginal microecosystem detection, wherein the primer-probe combination comprises primers and probes for target gene detection against Lactobacillus crispatus (LC), Lactobacillus gasseri (LG), Lactobacillus jensenii (LJ), Lactobacillus iners (LI), Gardnerella vaginalis (GV), Candida albicans (CA) and Trichomonas vaginalis (TV), and the probes are used for melting curve analysis. The kit comprises the primer-probe combination. The combination of specific primers and probes enables effective amplification of specific target genes of the above different microorganisms and melting curve analysis performed on the amplified products thereof, making detection of the above seven microorganisms in a single tube possible, thus achieving the advantages of high specificity, short time consuming, high sensitivity, comprehensive coverage of detection sites, etc.Type: GrantFiled: January 10, 2023Date of Patent: April 30, 2024Assignee: BEIJING ORIGIN-POLY BIO-TEC CO., LTD.Inventors: Linhai Wang, Pei Liu
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Patent number: 11959142Abstract: Provided herein are methods of detecting circulating tumor DNA, cancer cell mutations, and/or cancer cells harboring one or more cancer cell mutations. In some embodiments, methods provided herein include detecting one or more genetic alterations in cell-free DNA. In some embodiments, methods provided herein for detecting one or more genetic alterations in cell-free DNA can be performed when the subject is not known to harbor a cancer cell and/or a cancer cell mutation (e.g., when the subject is not known to harbor a cancer cell having the cancer cell mutation).Type: GrantFiled: May 3, 2018Date of Patent: April 16, 2024Assignee: The Johns Hopkins UniversityInventors: Victor Velculescu, Jillian A. Phallen
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Patent number: 11939623Abstract: A method of use and reagent kit for nucleic acid stabilization and room temperature transport of SARS-CoV-2 and other respiratory viruses, followed by rapid identification are disclosed. Using the methods and compositions herein, molecular diagnostics or detection of SARS-CoV-2 and other respiratory viruses from the biological sample is performed without extraction or purification of viral DNA or RNA.Type: GrantFiled: September 14, 2020Date of Patent: March 26, 2024Assignee: GenoSUR, LLCInventors: Matías Ricardo Gutiérrez Mostafá, Chantal Loretto Márquez Badilla, Ana Cecilia Morán
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Patent number: 11932911Abstract: Provided herein are compositions and methods useful for the detection of MTB. In particular, provided herein are kits, reagents, reaction mixtures, and methods involving such for nucleic acid amplification and detection procedures, which specifically and sensitively detect MTB in samples.Type: GrantFiled: March 10, 2021Date of Patent: March 19, 2024Assignee: Abbott Molecular, Inc.Inventors: Ning Tang, Gregor Leckie, Vihanga Pahalawatta, Andrea Frank, John Lampinen
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Patent number: 11919006Abstract: A generic point of care based portable device and method thereof as a platform technology for detecting pathogenic infection via nucleic acid based testing achieving sample-to-result integration, comprising the following interconnected stand-alone modules: a thermal unit for executing piece-wise isothermal reactions in a pre-programmable concomitant fashion without necessitating in-between operative intervention; a colorimetric detection unit seamlessly interfaced with smartphone-app based analytics for detecting the target analyte.Type: GrantFiled: March 16, 2021Date of Patent: March 5, 2024Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPURInventors: Nandita Kedia, Sujay Kumar Biswas, Saptarshi Banerjee, Aditya Bandopadhyay, Arindam Mondal, Suman Chakraborty
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Patent number: 11913065Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.Type: GrantFiled: December 12, 2019Date of Patent: February 27, 2024Assignee: Guardent Health, Inc.Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
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Patent number: 11913082Abstract: Methods are provided for a sensitive and specific assay for the determination of viral load and genotyping of RSV in a biological sample. Compositions and kits for use in the methods also are provided, including optimized primers for the amplification of and detection of the RSV open reading frames from subtypes A and B, and probes for distinguishing between the subtypes. Also provided are methods for amplifying and sequencing an open reading from of an RSV F protein, and compositions and kits for use in the methods.Type: GrantFiled: June 22, 2020Date of Patent: February 27, 2024Assignee: Laboratory Corporation of America HoldingsInventor: Justin Philip De La Cruz
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Patent number: 11913083Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.Type: GrantFiled: November 11, 2020Date of Patent: February 27, 2024Assignee: Becton, Dickinson and CompanyInventors: Chi Chen, Hugh J. Peck, Michael Porter, Gregory A. Richart, Ray A. McMillian
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Patent number: 11913070Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure provides methods of obtaining a bioelectronic signature based on current fluctuations that correspond to the activity of an enzyme-of-interest. As described herein, certain aspects of the bioelectronic signature can be used to determine the sequence of a biopolymer.Type: GrantFiled: February 24, 2021Date of Patent: February 27, 2024Assignee: Arizona Board of Regents on behalf of Arizona State UniversityInventor: Stuart Lindsay
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Patent number: 11913071Abstract: Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.Type: GrantFiled: January 13, 2023Date of Patent: February 27, 2024Assignee: Singular Genomics Systems, Inc.Inventors: Daan Witters, Eli N. Glezer, Allen Lipson
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Patent number: 11905553Abstract: Provided herein are compositions and methods for accurate and scalable Primary Template-Directed Amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for research, diagnostics, and treatment.Type: GrantFiled: January 28, 2019Date of Patent: February 20, 2024Assignee: ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC.Inventors: Charles Gawad, John Easton, Veronica Gonzalez-Pena
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Patent number: 11898200Abstract: The present disclosure discloses a method for detecting single strand breaks (SSBs) in DNA based on the following steps. First, DNA of interest is fragmented with a method that generates 3? ends that cannot be tailed. Second, the available 3? ends of the fragmented DNA corresponding to the pre-existing breaks are tailed. Third, SSBs are captured and their positions are identified genome-wide based on the following steps: (1) the tailed fragments are linearly amplified using a chimeric 5?-DNA-RNA-3? primer; (2) the products of primer extension are tailed at the 3? ends; (3) the desired products are amplified by PCR with oligonucleotides containing Illumina® adaptor sequences complementary to both tails and subjected to next-generation sequencing (NGS); 4) finally, positions of SSBs are revealed through the analysis of sequencing results.Type: GrantFiled: September 8, 2020Date of Patent: February 13, 2024Assignee: Huaqiao UniversityInventors: Philipp Kapranov, Huifen Cao, Lorena Salazar-García, Fan Gao, Dongyang Xu, Ye Cai, Xueer Han, Fang Wang, Lu Tang
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Patent number: 11866774Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.Type: GrantFiled: December 8, 2020Date of Patent: January 9, 2024Assignee: BioFire Diagnostics, LLCInventors: Kirk M. Ririe, Mark Aaron Poritz, Randy P. Rasmussen
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Patent number: 11859245Abstract: A primer set for detecting telomerase activity, the primer set including a first primer set or a second primer set. The first primer set includes: an upstream primer selected from MTS; and a downstream primer selected from the group consisting of ACX-M4, Beacon ACX62-2C, and Beacon ACX62-10. The second primer set includes: an upstream primer selected from STS or CTS; and a downstream primer selected from the group consisting of ACX, CXT, ACX-M4, Beacon ACX62-2C, or Beacon ACX62-10. The sequences of the primers ACX, CXT, ACX-M4, Beacon ACX62-2C, Beacon ACX62-10, STS, CTS and MTS are shown as SEQ ID NOs: 1 to 8, respectively.Type: GrantFiled: July 27, 2020Date of Patent: January 2, 2024Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Zheng Tan, Kewei Zheng, Yuhua Hao