Patents Examined by Cynthia B. Wilder
  • Patent number: 12123052
    Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.
    Type: Grant
    Filed: December 22, 2020
    Date of Patent: October 22, 2024
    Assignee: Cepheid
    Inventors: Joseph H. Smith, David H. Persing, Alan Wortman, Ronald Chang, David Swenson
  • Patent number: 12116624
    Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.
    Type: Grant
    Filed: December 11, 2023
    Date of Patent: October 15, 2024
    Assignee: Guardant Health, Inc.
    Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
  • Patent number: 12116640
    Abstract: Disclosed herein are methods, compositions, and devices for use in the early detection of cancer. The methods include preparing cell-free nucleic acid molecules from a subject for sequencing, sequencing a panel of regions in the cell-free nucleic acid molecules, and detecting one or more markers that are indicative of a cancer.
    Type: Grant
    Filed: February 8, 2024
    Date of Patent: October 15, 2024
    Assignee: Guardant Health, Inc.
    Inventors: Stefanie Ann Ward Mortimer, AmirAli Talasaz, Darya Chudova, Helmy Eltoukhy
  • Patent number: 12099057
    Abstract: Methods and systems are directed to multiplex detection of a bacterial pathogen in a sample. A first biotinylated lysin-derived cell wall binding domain is complexed with an avidin layer on a surface. A first bacterial pathogen detection complex including a second biotinylated lysin-derived cell wall binding domain, a detection domain, and an avidin linker complexed between the cell wall binding domain and the detection domain is also provided. The cell wall binding domains are derived from an endolysin, autolysin, bacteriocin, or exolysin, and are configured to bind a cell wall of a target bacterial pathogen. The detection domain includes one or more enzymes, fluorescent material, or DNA for emitting a signal for detection. Target bacterial pathogens present in a sample can thus be detected in a sandwich assay exhibiting increased selectivity and reduced limit of detection relative to traditional ELISA.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: September 24, 2024
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Seok-Joon Kwon, Jonathan Seth Dordick, Domyoung Kim, Jungbae Kim, Inseon Lee
  • Patent number: 12084724
    Abstract: The present disclosure relates to methods for detecting and targeting genomic rearrangements, in particular gene fusion events, by targeting a DNA molecule of interest with a set or pool of primers, wherein the forward primers and reverse primers produce a PCR amplification product when a genomic rearrangement is present. The present disclosure also relates to methods of bioinformatic analysis to determine whether or not the detection of an amplification product from the selective PCR is actually indicative of the presence of a gene fusion. The present disclosure also related to related methods of diagnosis and treatment of diseases and conditions associated with such genomic rearrangements, in particular cancers, such as lung cancer.
    Type: Grant
    Filed: October 5, 2021
    Date of Patent: September 10, 2024
    Assignee: INIVATA LTD.
    Inventors: Samuel Woodhouse, Stefanie Lensing, Tim Forshew, Vincent Plagnol, Matthew Edward Smith, Karen Howarth, Michael Epstein
  • Patent number: 11987847
    Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.
    Type: Grant
    Filed: December 14, 2020
    Date of Patent: May 21, 2024
    Assignees: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, EXACT SCIENCES CORPORATION
    Inventors: David A. Ahlquist, William R. Taylor, Douglas W. Mahoney, Graham P. Lidgard, Hatim T. Allawi
  • Patent number: 11970747
    Abstract: Disclosed in the present invention are a primer-probe combination and a kit for vaginal microecosystem detection, wherein the primer-probe combination comprises primers and probes for target gene detection against Lactobacillus crispatus (LC), Lactobacillus gasseri (LG), Lactobacillus jensenii (LJ), Lactobacillus iners (LI), Gardnerella vaginalis (GV), Candida albicans (CA) and Trichomonas vaginalis (TV), and the probes are used for melting curve analysis. The kit comprises the primer-probe combination. The combination of specific primers and probes enables effective amplification of specific target genes of the above different microorganisms and melting curve analysis performed on the amplified products thereof, making detection of the above seven microorganisms in a single tube possible, thus achieving the advantages of high specificity, short time consuming, high sensitivity, comprehensive coverage of detection sites, etc.
    Type: Grant
    Filed: January 10, 2023
    Date of Patent: April 30, 2024
    Assignee: BEIJING ORIGIN-POLY BIO-TEC CO., LTD.
    Inventors: Linhai Wang, Pei Liu
  • Patent number: 11959142
    Abstract: Provided herein are methods of detecting circulating tumor DNA, cancer cell mutations, and/or cancer cells harboring one or more cancer cell mutations. In some embodiments, methods provided herein include detecting one or more genetic alterations in cell-free DNA. In some embodiments, methods provided herein for detecting one or more genetic alterations in cell-free DNA can be performed when the subject is not known to harbor a cancer cell and/or a cancer cell mutation (e.g., when the subject is not known to harbor a cancer cell having the cancer cell mutation).
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: April 16, 2024
    Assignee: The Johns Hopkins University
    Inventors: Victor Velculescu, Jillian A. Phallen
  • Patent number: 11939623
    Abstract: A method of use and reagent kit for nucleic acid stabilization and room temperature transport of SARS-CoV-2 and other respiratory viruses, followed by rapid identification are disclosed. Using the methods and compositions herein, molecular diagnostics or detection of SARS-CoV-2 and other respiratory viruses from the biological sample is performed without extraction or purification of viral DNA or RNA.
    Type: Grant
    Filed: September 14, 2020
    Date of Patent: March 26, 2024
    Assignee: GenoSUR, LLC
    Inventors: Matías Ricardo Gutiérrez Mostafá, Chantal Loretto Márquez Badilla, Ana Cecilia Morán
  • Patent number: 11932911
    Abstract: Provided herein are compositions and methods useful for the detection of MTB. In particular, provided herein are kits, reagents, reaction mixtures, and methods involving such for nucleic acid amplification and detection procedures, which specifically and sensitively detect MTB in samples.
    Type: Grant
    Filed: March 10, 2021
    Date of Patent: March 19, 2024
    Assignee: Abbott Molecular, Inc.
    Inventors: Ning Tang, Gregor Leckie, Vihanga Pahalawatta, Andrea Frank, John Lampinen
  • Patent number: 11919006
    Abstract: A generic point of care based portable device and method thereof as a platform technology for detecting pathogenic infection via nucleic acid based testing achieving sample-to-result integration, comprising the following interconnected stand-alone modules: a thermal unit for executing piece-wise isothermal reactions in a pre-programmable concomitant fashion without necessitating in-between operative intervention; a colorimetric detection unit seamlessly interfaced with smartphone-app based analytics for detecting the target analyte.
    Type: Grant
    Filed: March 16, 2021
    Date of Patent: March 5, 2024
    Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
    Inventors: Nandita Kedia, Sujay Kumar Biswas, Saptarshi Banerjee, Aditya Bandopadhyay, Arindam Mondal, Suman Chakraborty
  • Patent number: 11913065
    Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.
    Type: Grant
    Filed: December 12, 2019
    Date of Patent: February 27, 2024
    Assignee: Guardent Health, Inc.
    Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
  • Patent number: 11913082
    Abstract: Methods are provided for a sensitive and specific assay for the determination of viral load and genotyping of RSV in a biological sample. Compositions and kits for use in the methods also are provided, including optimized primers for the amplification of and detection of the RSV open reading frames from subtypes A and B, and probes for distinguishing between the subtypes. Also provided are methods for amplifying and sequencing an open reading from of an RSV F protein, and compositions and kits for use in the methods.
    Type: Grant
    Filed: June 22, 2020
    Date of Patent: February 27, 2024
    Assignee: Laboratory Corporation of America Holdings
    Inventor: Justin Philip De La Cruz
  • Patent number: 11913083
    Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.
    Type: Grant
    Filed: November 11, 2020
    Date of Patent: February 27, 2024
    Assignee: Becton, Dickinson and Company
    Inventors: Chi Chen, Hugh J. Peck, Michael Porter, Gregory A. Richart, Ray A. McMillian
  • Patent number: 11913070
    Abstract: The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure provides methods of obtaining a bioelectronic signature based on current fluctuations that correspond to the activity of an enzyme-of-interest. As described herein, certain aspects of the bioelectronic signature can be used to determine the sequence of a biopolymer.
    Type: Grant
    Filed: February 24, 2021
    Date of Patent: February 27, 2024
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventor: Stuart Lindsay
  • Patent number: 11913071
    Abstract: Disclosed herein, inter alia, are substrates, kits, and efficient methods of preparing and sequencing two or more regions of a double-stranded polynucleotide.
    Type: Grant
    Filed: January 13, 2023
    Date of Patent: February 27, 2024
    Assignee: Singular Genomics Systems, Inc.
    Inventors: Daan Witters, Eli N. Glezer, Allen Lipson
  • Patent number: 11905553
    Abstract: Provided herein are compositions and methods for accurate and scalable Primary Template-Directed Amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for research, diagnostics, and treatment.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: February 20, 2024
    Assignee: ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC.
    Inventors: Charles Gawad, John Easton, Veronica Gonzalez-Pena
  • Patent number: 11898200
    Abstract: The present disclosure discloses a method for detecting single strand breaks (SSBs) in DNA based on the following steps. First, DNA of interest is fragmented with a method that generates 3? ends that cannot be tailed. Second, the available 3? ends of the fragmented DNA corresponding to the pre-existing breaks are tailed. Third, SSBs are captured and their positions are identified genome-wide based on the following steps: (1) the tailed fragments are linearly amplified using a chimeric 5?-DNA-RNA-3? primer; (2) the products of primer extension are tailed at the 3? ends; (3) the desired products are amplified by PCR with oligonucleotides containing Illumina® adaptor sequences complementary to both tails and subjected to next-generation sequencing (NGS); 4) finally, positions of SSBs are revealed through the analysis of sequencing results.
    Type: Grant
    Filed: September 8, 2020
    Date of Patent: February 13, 2024
    Assignee: Huaqiao University
    Inventors: Philipp Kapranov, Huifen Cao, Lorena Salazar-García, Fan Gao, Dongyang Xu, Ye Cai, Xueer Han, Fang Wang, Lu Tang
  • Patent number: 11866774
    Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.
    Type: Grant
    Filed: December 8, 2020
    Date of Patent: January 9, 2024
    Assignee: BioFire Diagnostics, LLC
    Inventors: Kirk M. Ririe, Mark Aaron Poritz, Randy P. Rasmussen
  • Patent number: 11859245
    Abstract: A primer set for detecting telomerase activity, the primer set including a first primer set or a second primer set. The first primer set includes: an upstream primer selected from MTS; and a downstream primer selected from the group consisting of ACX-M4, Beacon ACX62-2C, and Beacon ACX62-10. The second primer set includes: an upstream primer selected from STS or CTS; and a downstream primer selected from the group consisting of ACX, CXT, ACX-M4, Beacon ACX62-2C, or Beacon ACX62-10. The sequences of the primers ACX, CXT, ACX-M4, Beacon ACX62-2C, Beacon ACX62-10, STS, CTS and MTS are shown as SEQ ID NOs: 1 to 8, respectively.
    Type: Grant
    Filed: July 27, 2020
    Date of Patent: January 2, 2024
    Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Zheng Tan, Kewei Zheng, Yuhua Hao