Patents Examined by Cynthia B. Wilder
  • Patent number: 11111531
    Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.
    Type: Grant
    Filed: July 11, 2019
    Date of Patent: September 7, 2021
    Assignee: TANGEN BIOSCIENCES, INC.
    Inventors: John R. Nobile, John Davidson
  • Patent number: 11104954
    Abstract: The present disclosure relates to a method for isolating and amplifying a subject's mitochondrial deoxyribonucleic acid (mtDNA). The method comprises the steps of: isolating cell free deoxyribonucleic acid (cf-DNA) from a plasma sample obtained from the subject; and amplifying the mtDNA within the isolated cfDNA using a polymerase chain reaction with a first primer pair and a second primer pair. The first primer is selected from SEQ ID NO: 1 and SEQ ID NO: 2 and the second primer pair is selected from SEQ ID NO: 3 and SEQ ID NO: 4.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: August 31, 2021
    Assignees: M.A.G.I.C. Clinic Ltd., The Governors of the University of Alberta
    Inventors: Aneal Khan, Christopher Newell, Stacey Hume, Steven Greenway
  • Patent number: 11098301
    Abstract: The present disclosure is directed to systems, devices and methods for nucleic acid or protein purification and imaging. A system is provided including a cartridge comprising a sample input area configured to hold a sample, comprising a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with probes and a plurality of non-hybridized probes. The cartridge may also include a first binding chamber configured with first magnetic beads to receive and bind the sample, a first elution channel configured to receive the first magnetic beads and elute the sample from the first magnetic beads, a second binding chamber configured with second magnetic beads to receive and bind the sample, a second elution channel configured to receive the second magnetic beads and elute the sample from the second magnetic beads, and a binding area configured to receive the eluted sample and hold molecules for imaging.
    Type: Grant
    Filed: February 11, 2019
    Date of Patent: August 24, 2021
    Assignee: NanoString Technologies, Inc.
    Inventors: Dwayne Dunaway, Rustem Khafizov, Qian Mei, Lucas Dennis, Michael Krouse, Joseph M. Beechem, Isaac Sprague
  • Patent number: 11098370
    Abstract: A group of inventions belongs to medicine, specifically to the laboratory diagnostics, and concerns the diagnostics of diseases accompanied by the increased cell death and a kit for its performance. The human blood plasma sample is taken, then the real-time polymerase chain reaction is performed. Coefficient is calculated on basis value of polymerase chain reaction and it is compared with reference value. A disease accompanied by the increased cell death is diagnosed when value of coefficient is less than the reference value. The invention group allows to carry out the minimal invasive diagnostics of diseases accompanied by the increased cell death with high sensitivity, at any stages of disease including the early stages, at which the clinical signs are absent.
    Type: Grant
    Filed: February 3, 2017
    Date of Patent: August 24, 2021
    Assignee: LIMITED LIABILITY COMPANY “BIOMARKER-RU”
    Inventor: Anatoly Alexandrovich Melnikov
  • Patent number: 11091796
    Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.
    Type: Grant
    Filed: October 13, 2020
    Date of Patent: August 17, 2021
    Assignee: GUARDANT HEALTH, INC.
    Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
  • Patent number: 11091797
    Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.
    Type: Grant
    Filed: October 14, 2020
    Date of Patent: August 17, 2021
    Assignee: GUARDANT HEALTH, INC.
    Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
  • Patent number: 11078539
    Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.
    Type: Grant
    Filed: March 26, 2015
    Date of Patent: August 3, 2021
    Assignees: Mayo Foundation for Medical Education and Research, Exact Sciences Development Company, LLC
    Inventors: David A. Ahlquist, William R. Taylor, Douglas W. Mahoney, Graham P. Lidgard, Hatim T. Allawi
  • Patent number: 11071963
    Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: July 27, 2021
    Assignee: Gen9, Inc.
    Inventors: Joseph Jacobson, Larry Li-Yang Chu
  • Patent number: 11066700
    Abstract: According to an exemplary embodiment of the present application, provided is a nucleic acid complex pair, which includes a first nucleic acid complex including a first determinant and a second tag; and a second nucleic acid complex including a second determinant and a second tag, wherein the first determinant includes a forward primer for first target DNA, the second determinant includes a reverse primer for the first target DNA, the first tag includes a base sequence complementary to the base sequence of the second tag, and the second tag includes a base sequence complementary to the base sequence of the first tag.
    Type: Grant
    Filed: January 24, 2020
    Date of Patent: July 20, 2021
    Assignee: MULTILEX, INC.
    Inventors: Yong Tae Kim, Jun Hye Moon
  • Patent number: 11066698
    Abstract: Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: July 20, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Dianna Maar, Samantha Cooper, Wei Yang
  • Patent number: 11060141
    Abstract: The present application provides multiplex drop-off digital polymerase chain reaction (dPCR) assays, methods, systems, and kits. The methods described herein are useful in a variety of applications, such as detection of microsatellite instability and quantification of site-specific genome-edited products.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: July 13, 2021
    Assignee: STILLA TECHNOLOGIES
    Inventors: Barbara André, Rémi Dangla, Jordan Madic, Allison Mallory
  • Patent number: 11053551
    Abstract: A method of determining a probability that a human test subject has colorectal cancer as opposed to not having colorectal cancer is disclosed.
    Type: Grant
    Filed: January 30, 2018
    Date of Patent: July 6, 2021
    Assignee: StageZero Life Sciences Ltd.
    Inventors: Choong-Chin Liew, Samuel Chao
  • Patent number: 11035011
    Abstract: A method for identifying a risk factor for diseases, disorders or conditions, such as those caused by human immunodeficiency virus, using the polymerase chain reaction and specific primers. Methods for treating patients having these diseases, disorders or conditions by antimicrobial treatment of the risk factor by combined antiviral and antibacterial treatment or by sustaining or stimulating the subject's immune system. Methods for screening biological products including red blood cell preparations. Primers and methods for detecting nucleic acids or microbial agents associated with red blood cells, such as those associated with red blood cells in subjects infected with HIV and undergoing antiretroviral therapy.
    Type: Grant
    Filed: March 11, 2019
    Date of Patent: June 15, 2021
    Inventor: Luc Montagnier
  • Patent number: 11021757
    Abstract: There is a need for improved methods for determining the diagnosis and prognosis of patients with conditions, including autoimmune disease and cancer, especially lymphoid neoplasms, such as lymphomas and leukemias. Provided herein are methods for using DNA sequencing to identify personalized, or patient-specific biomarkers in patients with lymphoid neoplasms, autoimmune disease and other conditions. Identified biomarkers can be used to determine and/or monitor the disease state for a subject with an associated lymphoid disorder or autoimmune disease or other condition. In particular, the invention provides a sensitive method for monitoring lymphoid neoplasms that undergo clonal evolutions without the need to development alternative assays for the evolved or mutated clones serving as patient-specific biomarkers.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: June 1, 2021
    Assignee: Adaptive Biotechnologies Corporation
    Inventors: Malek Faham, Thomas Willis
  • Patent number: 10988801
    Abstract: Disclosed are methods and systems for enumeration of nucleic acids, including for the detection of rare events in a biological sample. In certain embodiments, the method may comprise arranging polynucleotides obtained from a biological sample to form a plurality of reaction sites, wherein each reaction site contains on average one polynucleotide; amplifying the polynucleotides in the plurality of reaction sites; determining by nucleic acid hybridization (i) a first number of first reaction sites containing a target nucleic acid sequence, or a portion thereof, and (ii) a second number of second reaction sites containing a reference nucleic acid sequence, or a portion thereof; comparing the first number of the first reaction sites to the second number of the second reaction sites to determine the relative amount of the target nucleic acid in the biological sample.
    Type: Grant
    Filed: December 5, 2018
    Date of Patent: April 27, 2021
    Assignee: Esoterix Genetic Laboratories, LLC
    Inventors: Thomas Scholl, Brant Hendrickson
  • Patent number: 10982265
    Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.
    Type: Grant
    Filed: April 20, 2020
    Date of Patent: April 20, 2021
    Assignee: GUARDANT HEALTH, INC.
    Inventors: AmirAli Talasaz, Stefanie Ann Ward Mortimer
  • Patent number: 10975418
    Abstract: The present invention provides a rapid and high-sensitivity method to detect virus by qPCR nucleic acid amplification. A viral sample containing a RNA virus such as a corona virus is mixed with a virus preservation solution and a nucleic acid amplification reaction preparation (which can be in a lyophilized powder) to prepare the PCR-sample solution. The virus preservation solution can preserve the viral sample and prevent RNA degradation at room temperature for an extended period of time. No viral nucleic acid extraction step is needed.
    Type: Grant
    Filed: August 4, 2020
    Date of Patent: April 13, 2021
    Assignee: JIANGSU COWIN BIOTECH CO., LTD
    Inventors: Chunxiang Wang, Jinhai Guo, Huanhuan Xiao
  • Patent number: 10975446
    Abstract: Provided herein are compositions and methods useful for the detection of MTB. In particular, provided herein are kits, reagents, reaction mixtures, and methods involving such for nucleic acid amplification and detection procedures, which specifically and sensitively detect MTB in samples.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: April 13, 2021
    Assignee: ABBOTT MOLECULAR INC.
    Inventors: Ning Tang, Gregor Leckie, Vihanga Pahalawatta, Andrea Frank, John Lampinen
  • Patent number: 10978175
    Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: April 13, 2021
    Assignee: KEYGENE N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
  • Patent number: 10954553
    Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.
    Type: Grant
    Filed: July 12, 2016
    Date of Patent: March 23, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Shoulian Dong, Chunmei Liu