Abstract: A method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes has the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier; b) analyte or group of analytes is incubated with a detection probe; c) then the solid carrier is washed to remove unbound detection probe; and subsequently, the amount of bound detection probe is determined.
Type:
Grant
Filed:
August 4, 2015
Date of Patent:
July 21, 2020
Assignee:
USTAV ORGANICKE CHEMIE A BIOCHEMIE AKADEMIE VED CR, V.V.I.
Inventors:
Vaclav Navratil, Pavel Sacha, Jiri Schimer, Jan Konvalinka, Pavel Majer
Abstract: The purpose of the present invention is to provide a method and kit for highly precise DNA typing, in which a high throughput sequencer is used and ambiguity derived from phase ambiguity is eliminated. The present invention provides a method for the DNA typing of HLA, which is characterized by comprising: (1) a step of preparing sets of primers which respectively hybridize specifically to an upstream region and a downstream region of at least 2 genes selected from genes belonging to HLA class I and HLA class II in a human genome sequence, and are capable of amplifying under the same PCR conditions; (2) a step of simultaneously amplifying said at least 2 genes in a test sample (DNA) using the sets of primers in a single container under the same PCR conditions; (3) a step of determining the nucleotide sequences of PCR amplified products; and (4) a step of optionally carrying out a homology search within a database.
Abstract: The present invention relates to a microfluidic system including a temperature controller and a thermoplastic microfluidic chip that enables rapid PCR in a PCR chamber of the microfluidic chip. Thermal control of the PCR chamber is achieved by applying voltage to heater electrodes patterned directly onto one layer of the microfluidic chip. The temperature controller adjusts the voltage applied to the heater electrodes by changing temperature controller parameters selected to minimize duration of each PCR cycle. Furthermore, simple operation of the microfluidic chip is provided through using an integrated passive capillary valve, requiring minimum operator intervention and eliminating the need for fluidic interfacing, pumping, or metering during chip loading.
Type:
Grant
Filed:
April 14, 2017
Date of Patent:
July 14, 2020
Assignee:
University of Maryland
Inventors:
Donald DeVoe, Eric Kendall, Alex Sposito
Abstract: A polymerase chain reaction (PCR) module is detachably combined with a reader system. The reader system includes a central processing unit (CPU) receiving a photo sensing signal to calculate gene amplification amount in real time and generating a temperature control signal based on a temperature signal and a temperature control information. The PCR module includes a photo sensor assembly, a partition wall, and an interface module. The photo sensor assembly includes a plurality of photo sensors and a temperature sensor. The photo sensors are arranged in an array shape to sense emission light generated from a specimen to generate the photo sensing signal. The partition wall is protruded from the photo sensor assembly to define a reaction space in which the specimen is received. The interface module is electrically connected to the photo sensor assembly to transmit the photo sensing signal and the temperature signal to the reader system.
Type:
Grant
Filed:
March 22, 2019
Date of Patent:
July 14, 2020
Assignee:
OPTOLANE TECHNOLOGIES INC.
Inventors:
DoYoung Lee, In Gyun Jeon, An Shik Choi, Kyung Hak Choi
Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.
Type:
Grant
Filed:
September 18, 2019
Date of Patent:
July 7, 2020
Assignee:
GUARDANT HEALTH, INC.
Inventors:
AmirAli Talasaz, Stefanie Ann Ward Mortimer
Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.
Type:
Grant
Filed:
August 23, 2017
Date of Patent:
June 23, 2020
Assignee:
10X Genomics, Inc.
Inventors:
Rajiv Bharadwaj, Tobias Daniel Wheeler, Kevin Ness, Benjamin Hindson
Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.
Type:
Grant
Filed:
June 27, 2018
Date of Patent:
June 23, 2020
Assignee:
GEN-PROBE INCORPORATED
Inventors:
Jijumon Chelliserry, Kui Gao, Jeffrey M. Linnen
Abstract: Methods are provided for a sensitive and specific assay for the determination of viral load and genotyping of RSV in a biological sample. Compositions and kits for use in the methods also are provided, including optimized primers for the amplification of and detection of the RSV open reading frames from subtypes A and B, and probes for distinguishing between the subtypes. Also provided are methods for amplifying and sequencing an open reading from of an RSV F protein, and compositions and kits for use in the methods.
Type:
Grant
Filed:
August 22, 2016
Date of Patent:
June 23, 2020
Assignee:
Laboratory Corporation of America Holdings
Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
Type:
Grant
Filed:
April 19, 2017
Date of Patent:
May 19, 2020
Assignees:
University of Utah Research Foundation, Biofire Defense, LLC
Inventors:
Luming Zhou, Carl T. Wittwer, Philip Seth Bernard, Virginie Dujols
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
Type:
Grant
Filed:
April 9, 2018
Date of Patent:
May 19, 2020
Assignee:
General Electric Company
Inventors:
Ryan Charles Heller, Erik Leeming Kvam, John Richard Nelson
Abstract: Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.
Type:
Grant
Filed:
July 11, 2018
Date of Patent:
May 12, 2020
Assignee:
LUMINEX CORPORATION
Inventors:
Dylan Hilmer Bird, Jesus Ching, Bruce A. Johnson, Keith E. Moravick, Bruce Richardson
Abstract: The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5? terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule.
Abstract: Provided are methods and systems for detecting formation of nucleotide-specific ternary complexes comprising a DNA polymerase, a nucleic acid, and a nucleotide complementary to the templated base of the primed template nucleic acid. The methods and systems facilitate determination of the next correct nucleotide without requiring chemical incorporation of the nucleotide into the primer. These results can even be achieved in procedures employing unlabeled, native nucleotides.
Abstract: Disclosed are a composition for PCR including polyethylene glycol-engrafted nano-sized graphene oxide (PEG-nGO), the composition for PCR being capable of increasing the efficiency and specificity of PCR and shortening PCR time, and a PCR method using the same.
Type:
Grant
Filed:
December 1, 2017
Date of Patent:
April 28, 2020
Assignee:
KONKUK UNIVERSITY INDUSTRIAL COOPERATION CORP
Inventors:
Dong Eun Kim, Hyo Ryoung Kim, Ah Ruem Baek
Abstract: The invention relates to methods of detecting a genetic variation in a genetic sample from a subject using labeled probes and counting the number of labels in the probes.
Type:
Grant
Filed:
August 11, 2017
Date of Patent:
April 21, 2020
Assignee:
Singular Bio, Inc.
Inventors:
Adrian Nielsen Fehr, Patrick James Collins, Jill Lyndon Herschleb, Hywel Bowden Jones
Abstract: Provided herein are methods for enriching a biological sample for a target nucleic acid, and analyzing the nucleic acid. In some cases, a biological sample is enriched for target nucleic acids associated with a cancer or tumor. In some cases, a biological sample is enriched for target nucleic acids, and the target nucleic acids vary in length. In some cases, one or more probes are used to enrich the biological sample for the target nucleic acid. In some cases, one or more probes hybridize to one or more ends of a target nucleic acid.
Abstract: Primer pair, kit and method for detecting Ehrlichia canis are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
Abstract: Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided.
Type:
Grant
Filed:
May 21, 2019
Date of Patent:
April 14, 2020
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Paul Giresi, Jason D. Buenrostro, Howard Y. Chang, William J. Greenleaf