Patents Examined by David Steadman
  • Patent number: 11597938
    Abstract: Disclosed herein are genetically modified microorganisms and related methods for enhanced utilization of oligosaccharides and improved productivity of compounds derived from the metabolism of the oligosaccharides. The microorganisms described herein have altered activities of plasma membrane ATPase protein (PMA1) and/or one or more extracellular glucose sensors, namely, sucrose non-fermenting protein (SNF3), restores glucose transport protein (RGT2), and G protein-coupled receptor 1 protein (GPR1). These genetic modifications provide the microorganisms an increased ability to utilize an oligosaccharide to produce a compound of interest, particularly, tagatose, 2?-fucosyllactose, and psicose. Methods of culturing the microorganisms in the presence of such oligosaccharides to produce the products of interest are also provided.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: March 7, 2023
    Assignee: Zimitech, Inc.
    Inventors: Kulika Chomvong, James Harrison Doudna Cate, Yong-Su Jin
  • Patent number: 11584913
    Abstract: The present invention discloses a strain of Pseudomonas aeruginosa with monomethylamine degradability and the application thereof. This strain, named Pseudomonas aeruginosa GDUTAN1, was deposited on May 24, 2017 in the China Center for Type Culture Collection in Wuhan University, Wuhan City, Hubei Province with a deposit number of CCTCC NO.: M 2017283. This Pseudomonas aeruginosa GDUTAN1 was Gram-negative and rod-like, and round, green and opaque in the colony morphology, having a diameter of 1-2 mm. The Pseudomonas aeruginosa GDUTAN1 of the present invention can be applied to environmental remediation, degrading monomethylamine in the environment at a high degradation efficiency. When it degrades monomethylamine for 96 h at a substrate concentration of 50-140 mg/L, the degradation efficiency can reach more than 99%.
    Type: Grant
    Filed: March 20, 2018
    Date of Patent: February 21, 2023
    Inventors: Taicheng An, Shiai Li, Guiying Li
  • Patent number: 11584944
    Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) or glycolic acid (GA), or MEG and one or more co-product, from one or more pentose and/or hexose sugars. Also provided are methods of producing MEG (or GA), or MEG (or GA) and one or more co-product, from one or more pentose and/or hexose sugars using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA), or MEG and one or more co-product.
    Type: Grant
    Filed: February 20, 2020
    Date of Patent: February 21, 2023
    Assignee: BRASKEM S.A.
    Inventors: Daniel Johannes Koch, Lucas Pederson Parizzi, Felipe Galzerani, Jean Marie Francois, Sophie Lajus
  • Patent number: 11578315
    Abstract: Variant guinea pig L-asparaginases which are truncated and humanized are described as are fusion proteins containing the L-asparaginase and use of the L-asparaginases in the treatment of cancers such as acute lymphoblastic leukemia and acute myeloid leukemia.
    Type: Grant
    Filed: August 10, 2018
    Date of Patent: February 14, 2023
    Assignees: The Board of Trustees of the University of Illinois, The United States Government as represented by the Department of Veterans Affairs
    Inventors: Arnon Lavie, Hien-Anh Nguyen, Amanda Schalk
  • Patent number: 11566238
    Abstract: Compositions and methods are provided for modulating growth of a genetically modified bacterial cell present in a human organ, for modulating growth of a genetically modified bacterial cell in an organ (e.g., gut), for displacing at least a portion of a population of bacterial cells in an organ, and for facilitating gut colonization by a genetically modified bacterial cell. Also provided are genetically modified bacterial cells, e.g., cells that include a heterologous carbohydrate-utilization gene or gene set that provides for the ability to utilize as a carbon source a rare carbohydrate of interest that is utilized as a carbon source by less than 50% of bacterial cells present in a human microbiome.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: January 31, 2023
    Assignees: The Board of Trustees of the Leland Stanford Junior University, Novome Biotechnologies, Inc.
    Inventors: Justin L. Sonnenburg, Weston R. Whitaker, Elizabeth Stanley, William C. DeLoache
  • Patent number: 11566267
    Abstract: Provided is a method for producing an organic substance from a syngas by microbial fermentation, wherein only the solid component can be efficiently separated from an organic substance-containing liquid obtained by microbial fermentation to reduce the content of microorganisms, etc. Disclosed is a method for producing an organic substance from a syngas containing carbon monoxide by microbial fermentation, which comprises a microbial fermentation step wherein the syngas is fed to a microbial fermenting vessel and a liquid containing an organic substance is obtained by microbial fermentation, a solid-liquid separation step wherein the organic substance-containing liquid is separated into a solid component containing microorganisms and a liquid component containing an organic substance, and an extraction step wherein the organic substance-containing liquid is extracted from the liquid component, wherein the organic substance-containing liquid is heated to 40° C.
    Type: Grant
    Filed: March 3, 2019
    Date of Patent: January 31, 2023
    Inventors: Satoshi Shimizu, Kokoro Hamachi, Kazuto Natsuyama, Kanetomo Sato
  • Patent number: 11560551
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.
    Type: Grant
    Filed: December 11, 2020
    Date of Patent: January 24, 2023
    Inventors: Yougen Li, Ellen D. Semke, Qiong Cheng, Jian Ping Lai
  • Patent number: 11549130
    Abstract: An object of the present invention is to provide an enzyme showing random transesterification ability, which is suitable for food applications. Provided is a random transesterification lipase having high heat resistance in oils and fats.
    Type: Grant
    Filed: December 27, 2018
    Date of Patent: January 10, 2023
    Assignee: AMANO ENZYME INC.
    Inventors: Hiroki Fujioka, Saki Norifune, Tatsuya Hamada, Tetsuya Takahashi, Yusuke Tanaka, Toru Katase
  • Patent number: 11542489
    Abstract: The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.
    Type: Grant
    Filed: July 27, 2021
    Date of Patent: January 3, 2023
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Greiner-Stoeffele, Stefan Schoenert
  • Patent number: 11542481
    Abstract: Disclosed herein are methods for N-demethylating an N-methylated compound using an enzymatic reaction, rather than, e.g. a chemical modification. Also provided herein are enzymes for performing the reaction.
    Type: Grant
    Filed: January 18, 2018
    Date of Patent: January 3, 2023
    Inventors: Toni Kutchan, Megan Augustin
  • Patent number: 11535831
    Abstract: Methods and compositions for modifying glycans (e.g., glycans expressed on the surface of live cells or cell particles) are provided herein.
    Type: Grant
    Filed: May 8, 2020
    Date of Patent: December 27, 2022
    Inventor: Robert Sackstein
  • Patent number: 11535641
    Abstract: The invention relates to the field of nutritional ingredients, in particular to methods for producing hypoallergenic galacto-oligosaccharides (GOS) and the use thereof in food and drink items. Provided is the use of a beta-galactosidase (EC derived from Cryptococcus terrestris (recently renamed Papiliotrema terrestris) in the production of a hypoallergenic GOS preparation having a reduced capacity to induce an allergic response in a subject.
    Type: Grant
    Filed: December 27, 2019
    Date of Patent: December 27, 2022
    Assignee: FrieslandCampina Nederland B.V.
    Inventors: Dirk Andries Groeneveld, Bas Johan Henri Kuipers, Bernardina Johanna Martina Delsing, Linqiu Cao
  • Patent number: 11530397
    Abstract: The present invention relates to polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.
    Type: Grant
    Filed: October 16, 2020
    Date of Patent: December 20, 2022
    Assignee: Novozymes A/S
    Inventors: Morten Gjermansen, Klaus Gori, Henrik Marcus Geertz-Hansen, Jesper Salomon, Mary Ann Stringer, Thomas Holberg Blicher, Nikolaj Spodsberg
  • Patent number: 11530391
    Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.
    Type: Grant
    Filed: April 8, 2019
    Date of Patent: December 20, 2022
    Assignee: BRASKEM S.A.
    Inventors: Verônica Leite Queiroz, Lucas Pedersen Parizzi, Iuri Estrada Gouvea, Debora Noma Okamoto, Rafael Victório Carvalho Guido, Alessandro Silva Nascimento, Igor Polikarpov
  • Patent number: 11524981
    Abstract: The present disclosure relates to a protein variant having a tryptophan-exporting activity, an L-tryptophan-producing microorganism expressing the protein variant, and a method for producing L-tryptophan using the microorganism.
    Type: Grant
    Filed: March 20, 2020
    Date of Patent: December 13, 2022
    Inventors: Moo Young Jung, Hyun Ah Kim, Chang Il Seo, Imsang Lee, Ji-won Kim, Tae Yeon Kim, Sung Kwang Son, Ki Yong Cheong
  • Patent number: 11519011
    Abstract: The present invention provides methods and compositions for increasing lignin degradation to produce a biological product. Also provided are methods for increasing expression of laccase in a bacterial species to produce increased lignin degradation. Also provided are bacterial cells and commodities or commodity produces produced from such methods.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: December 6, 2022
    Assignee: The Texas A&M University System
    Inventors: Shuhua Yuan, Shangxian Xie, Xin Wang
  • Patent number: 11510413
    Abstract: The present invention relates to a polypeptide having lipolytic enzyme activity, selected from the group consisting of: (a) a polypeptide having at least 65% sequence identity to amino acids 21 to 309 of SEQ ID NO: 1; (b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions with the polypeptide coding sequence of SEQ ID NO: 2; (c) a polypeptide encoded by a polynucleotide having at least 65% sequence identity to the polypeptide coding sequence of SEQ ID NO: 2; and (d) a fragment of the polypeptide of (a), (b) or (c) that has lipolytic enzyme activity.
    Type: Grant
    Filed: June 3, 2021
    Date of Patent: November 29, 2022
    Assignees: Novozymes A/S, Puratos NV/SA
    Inventors: Henrik Oestdal, Sara Maria Landvik, Robert Piotr Olinski, Evelien Agache, Bruno Van Winckel, Filip Arnaut
  • Patent number: 11504418
    Abstract: Disclosed herein is an endotoxin-free asparaginase enzyme. Also disclosed are methods of using the disclosed enzyme to treat subjects with a disease treatable by depletion of asparagine. For example, the disclosed endotoxin-free asparaginase enzyme is useful in the treatment or the manufacture of a medicament for use in the treatment of acute lymphoblastic leukemia (ALL) in both adults and children, as well as other conditions where asparagine depletion is expected to have a useful effect.
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: November 22, 2022
    Inventors: George E. Pierce, Sidney A. Crow
  • Patent number: 11505587
    Abstract: Method of preparing a keratin-based biomaterial is provided. The method comprises a) reacting keratin with a polymer having at least one of an amine and carboxylic functional group in the presence of a carbodiimide cross-linking agent to form a cross-linked keratin-polymer material; and b) freeze drying the cross-linked keratin-polymer material to form the keratin-based bio-material. A keratin-based biomaterial thus prepared is also provided.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: November 22, 2022
    Inventors: Kee Woei Ng, Pietradewi Hartrianti
  • Patent number: 11499175
    Abstract: A polypeptide includes, in the amino acid sequence of SEQ ID NO: 1 or a similar sequence, at least one specific amino acid substitution on at least one of the 14th, 37th, 209th, 293rd, and 319th amino acid residues from the N-terminal of the amino acid sequence of SEQ ID NO: 1. A polynucleotide, an expression cassette, a vector, and a transformant include a base sequence encoding the amino acid sequence of the polypeptide. A method of producing the polypeptide and a method of producing 2-deoxy-scyllo-inosose are also provided.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: November 15, 2022
    Inventors: Hiroaki Takaku, Harutake Yamazaki, Mitsufumi Wada, Daisuke Miyazawa