Patents Examined by David W Berke-Schlessel
  • Mass spectrometry ionization based-assay for the detection of enzyme activity and/or presence
    Patent number: 11913059
    Abstract: An apparatus and method for detecting an analyte of interest using paper spray mass spectrometry includes a spray material; a sample on the spray material including an enzyme of interest; a solvent to hydrate the sample, promote enzymatic activity, and extract analytes of interest from the sample; a substrate specific to the enzyme of interest, wherein any of the spray material and the solvent includes the substrate; a voltage source to apply a voltage to the spray material to create charged droplets of a mixture containing the sample; and a mass spectrometer to perform spectrometry on the droplets to perform any of: identify the analytes of interest in the sample; and measure a level of inhibition in any enzymes contained in the sample.
    Type: Grant
    Filed: October 26, 2021
    Date of Patent: February 27, 2024
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Trevor G Glaros, Elizabeth S Dhummakupt, Phillip M Mach, Daniel O Carmany
  • Hyperthermophilic aerobic fermentation inoculant prepared by using municipal sewage sludge and its method
    Patent number: 11898140
    Abstract: The present disclosure discloses a preparation method for hyperthermophilic aerobic fermentation inoculant prepared by using sewage sludge, the method includes the following steps: carrying out fermentation after the activation of hyperthermophilic aerobic bacteria, removing the supernatant from the fermentation products, and adding the protective agent and stirring until uniform, drying to obtain a product, pulverizing the product by a pulverizer, and sieving the product before sub-packing. The solution of the present disclosure has the following advantages.
    Type: Grant
    Filed: March 22, 2020
    Date of Patent: February 13, 2024
    Inventors: Shungui Zhou, Zhi Chen, Hanpeng Liao
  • Method for the production of thymocyte supernatant
    Patent number: 11891624
    Abstract: Herein is reported a method for producing a thymocyte supernatant comprising the steps of co-cultivating thymocytes and mononuclear cells at a cell ratio of at least 0.5:1.2 in the presence of phorbol-12-myristate-13-acetate and Phytohemagglutinin M for up to 60 hours, and separating the co-cultivation medium from the cells and thereby producing the thymocyte supernatant.
    Type: Grant
    Filed: October 2, 2019
    Date of Patent: February 6, 2024
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Sonja Offner, Markus Horvath, Ingried Saam
  • Chitin, hydrolysate and method for the production of one or more desired products from insects by means of enzymatic hydrolysis
    Patent number: 11891459
    Abstract: The invention relates to chitin, a hydrolysate and a method for the production of at least one desired product from insects. More specifically, the invention relates to a method for the production of at least one desired product from insects, comprising an insect cuticle pressing step, followed by the enzymatic hydrolysis of the insect cuticles using a proteolytic enzyme.
    Type: Grant
    Filed: December 30, 2015
    Date of Patent: February 6, 2024
    Inventors: Nathalie Berezina, Antoine Hubert, Fabrice Berro, Jean-Gabriel Levon, Karine Le Roux, Cecilia Socolsky, Lorena Sanchez, Sophie Laurent
  • Compositions and methods for improving the health of aquatic animals
    Patent number: 11883443
    Abstract: The present invention relates to compositions and methods for increasing the health of aquatic animals comprising one or more selected bacterial strains.
    Type: Grant
    Filed: April 9, 2014
    Date of Patent: January 30, 2024
    Assignee: Novozymes A/S
    Inventors: David Drahos, Christian Munch, Lene Venke Kofod
  • Biological indicator for determining the efficacy of a steam or heat sterilization process and its method of use
    Patent number: 11884960
    Abstract: The invention relates to a biological indicator for determining the efficacy of a steam or heat sterilization process, and its method. The biological indicator comprises microbial spores (a), at least one sensor protein exogenous to the microbial spores (b), one fluorophore (c), and a culture medium (d). The invention also refers to the method of use of this biological indicator.
    Type: Grant
    Filed: August 16, 2019
    Date of Patent: January 30, 2024
    Assignees: Terragene LLC, Terragene SA
    Inventors: Adrian Jesus Rovetto, Esteban Lombardia, Pablo Ravasi
  • sp. strains for efficient biomass production using greenhouse gases
    Patent number: 11884914
    Abstract: The invention is directed to Haematococcus sp. KAU-01 as well as to a culture medium for Haematococcus sp. KAU-01, and to methods for using this strain to process environmental pollutants such as gases generated by coal-fired plants.
    Type: Grant
    Filed: December 7, 2020
    Date of Patent: January 30, 2024
    Assignee: King Abdulaziz University
    Inventors: Adnan Jaman Turki, Md. Abu Affan, Salim Marzoog Al-Harbi
  • Glycan therapeutic compositions and related methods thereof
    Patent number: 11883422
    Abstract: Preparations of glycan therapeutics, pharmaceutical compositions, dietary supplements and medical foods thereof are provided, and methods of using said glycan therapeutics, e.g. in cancer therapy.
    Type: Grant
    Filed: June 30, 2021
    Date of Patent: January 30, 2024
    Assignee: DSM Nutritional Products, LLC
    Inventors: Geoffrey A. von Maltzahn, Yvonne J. Yamanaka, Jared Silverman, John Miles Milwid, Jacob Rosenblum Rubens, John M. Geremia
  • Method of screening for a substance that acts on a cell mass
    Patent number: 11873514
    Abstract: A method of screening for a substance that acts on a cell mass includes producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, adding a test substance to the cell mass, and evaluating an action of the test substance on the cell mass. The cell mass is produced by culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate and producing the cell mass of the primary cancer cells.
    Type: Grant
    Filed: December 23, 2022
    Date of Patent: January 16, 2024
    Assignee: LSI MEDIENCE CORPORATION
    Inventors: Hideaki Kakinuma, Yukiko Shimada, Hiroaki Inoue, Takashi Morikawa
  • Method and system for dissociating biological tissue into single cells using ultrasonic energy
    Patent number: 11866694
    Abstract: A method of dissociating single cells from a biological tissue sample is described herein, along with systems for performing such methods. The method includes generating one or more ultrasonic wave pulses using a transducer comprising one or more Fresnel Annular Sector Actuator (FASA) elements; and applying energy from the generated one or more ultrasonic wave pulses to a sample container holding the biological tissue sample through a coupling medium that couples the one or more FASA elements to the sample container to dissociate single cells from the biological tissue is described herein.
    Type: Grant
    Filed: October 1, 2020
    Date of Patent: January 9, 2024
    Assignee: Microsonic Systems Inc.
    Inventors: Vibhu Vivek, Poonam Sansanwal
  • Live biotherapeutics for the treatment of carbohydrate disorders
    Patent number: 11865151
    Abstract: The present invention provides method for treating a metabolic disorder, dietary intolerance, or malabsorption in a subject that causes an excess of a metabolite or a dietary substance, particularly a carbohydrate such as galactose or fructose, that the subject does not tolerate by administering an effective amount of an adaptively evolved yeast strain to the subject or to food that reduces the excess of the metabolite or dietary substance. Specifically, the invention is directed to methods for treating the metabolic disorder, such as galactosemia or fructosemia, in which an excess of the carbohydrate is present. The invention also provides isolated, adaptively evolved yeast strains that degrades a metabolite or dietary ingredient, such as galactose or fructose, and methods for preparing such isolated, adaptively evolved yeast strains.
    Type: Grant
    Filed: February 14, 2020
    Date of Patent: January 9, 2024
    Inventor: Genevieve Hansen
  • Strain in and method for producing psicose using same
    Patent number: 11859226
    Abstract: The present invention relates to a newly isolated bacterium belonging to the genus Microbacterium, a composition for producing psicose comprising the strain, and a method for producing psicose using the same.
    Type: Grant
    Filed: December 26, 2017
    Date of Patent: January 2, 2024
    Assignee: SAMYANG CORPORATION
    Inventors: Bu-Soo Park, Eun Jin Han, Sang-Hee Lee, Soun Gyu Kwon, Jin Ha Kim, Chong Jin Park
  • Bone marrow microfluidic devices and methods for preparing and using the same
    Patent number: 11845920
    Abstract: The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: December 19, 2023
    Assignees: President and Fellows of Harvard College, The General Hospital Corporation
    Inventors: David Benson Chou, Liliana S. Teixeira Moreira Leijten, Arianna Rech, Richard Novak, Donald E. Ingber, Yuka Milton, Viktoras Frismantas, Oren Levy
  • Methods of transfection using sonoporation
    Patent number: 11834650
    Abstract: The present invention relates to methods of transforming a cell by use of an acoustic transducer. In particular embodiments, the radiofrequency signal to the transducer can be tuned to provide optimal membrane disruption of the cell; and an agent, such as a nucleic acid, can then be delivered to the cell.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: December 5, 2023
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Anne Ruffing, Darren W. Branch, Ronald P. Manginell
  • Assays for improving automated antimicrobial susceptibility testing accuracy
    Patent number: 11835523
    Abstract: Phenotypic antimicrobial susceptibility testing (AST), the gold-standard diagnostic that indicates whether an antimicrobial will be clinically effective, often suffer the slowest times-to-result for the most resistant pathogens. Here we introduce novel assays to be performed in parallel with standard AST assays that enable rapid, same-shift reporting of AST results for a plurality of pathogens. The assays developed here are further capable of detecting resistance to carbapenems, the most powerful class of beta-lactams commonly used as “last-resort” antimicrobials.
    Type: Grant
    Filed: February 15, 2022
    Date of Patent: December 5, 2023
    Assignee: SELUX DIAGNOSTICS, INC.
    Inventors: Eric Stern, Kelly Flentie, Nicholas Phelan
  • Hematopoietic stem cells with improved properties
    Patent number: 11834678
    Abstract: A method of generating hematopoietic stem cells for transplantation is disclosed. The method comprising: (a) collecting hematopoietic stem cells; and (b) contacting the hematopoietic stem cells with an agent capable of decreasing an activity or expression of CD74 and/or of macrophage migration inhibitory factor (MIF), to thereby generate hematopoietic stem cells for transplantation, and wherein the hematopoietic stem cells are not transduced with a lentivirus. Methods of increasing mobilization of hematopoietic stem cells and methods of treatment are also provided.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: December 5, 2023
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Idit Shachar, Shirly Herman
  • Functional cortical organoids, methods of making, and uses thereof
    Patent number: 11821895
    Abstract: The disclosure provides methods of making functional cortical organoids from somatic cells and stem cells and methods of using the functional organoids.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: November 21, 2023
    Assignee: The Regents of the University of California
    Inventors: Alysson Renato Muotri, Priscilla Davidson Negraes, Cleber Trujillo, Pinar Mesci
  • Methods and compositions for preserving bacteria
    Patent number: 11814615
    Abstract: The disclosure provides methods and compositions for the preservation of bacteria.
    Type: Grant
    Filed: August 29, 2022
    Date of Patent: November 14, 2023
    Assignee: Vedanta Biosciences, Inc.
    Inventors: Daniel E. Couto, Shilpa Reddy, Jil Ulrich, Scott Michonski
  • Method for irradiating mammalian cells with electron beams and/or X-rays
    Patent number: 11802271
    Abstract: The present invention relates to a method for irradiating a population of mammalian cells comprising at least one target mammalian cell with electron beams and/or X-rays, characterized in that: (i) a composition comprising a population of mammalian cells is irradiated in vitro with electron beams and/or X-rays, the population of mammalian cells containing at least one target mammalian cell and the dose rate being within the range from 5 Gy/sec to 107 Gy/sec, and (ii) optionally viable target mammalian cells are isolated or enriched from the population of mammalian cells, and to agents obtainable thereby and to uses thereof.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: October 31, 2023
    Assignee: FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
    Inventors: Frank-Holm Rögner, Sebastian Ulbert, Jana Burkhardt, Javier Portillo, Jessy Schönfelder
  • Microbial test standard for use in infrared spectrometry
    Patent number: 11802833
    Abstract: The invention relates to a microbial test standard for use in infrared spectrometry which has at least two resealable vessels which are liquid-tight when closed, each of which contains a predefined amount of dried biomass of a microorganism. The microorganisms in the different vessels differ in at least one characteristic, which is selected in particular from the group comprising species, subspecies, strain, serovar, pathovar, toxivar and variety, and the difference manifests itself in a predefined intermicrobial spectral distance. The disclosure furthermore comprises a method of using the microbial test standard.
    Type: Grant
    Filed: February 4, 2021
    Date of Patent: October 31, 2023
    Inventors: Thomas Maier, Norman Mauder