Abstract: The present disclosure relates to the application of a viable cell monitor to the cryopreservation of plant cells. The viable cell monitor is, for the first time, used in a method for rapidly evaluating the effect of cell freezing-preservation. The effect of a cryoprotectant on cell preservation is evaluated through the change in the capacitance of viable cells before and after cell freezing and resuscitation. Viable cell electrodes can provide a quick and accurate characterization of viable cell concentrations. Combining the viable cell capacitance with the detection result of 2,3,4-triphenyl tetrazolium chloride (TTC), a rapid and quantitative evaluation of the effect of a freezing method on cell preservation can be achieved, thus overcoming the low effectiveness and non-quantification of previous cell preservation methods. Compared with traditional methods, the method of the present disclosure can improve the comprehensive score of cell cryopreservation by 5 times or more.

Abstract: The present disclosure relates to human facilitating cells (hFC), and methods of isolating, characterizing, and using such hFCs.

Abstract: The disclosure relates to isolated microorganisms—including novel strains of the microorganisms—microbial consortia, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and compositions comprising the same, in methods for modulating the production and yield of milk and milk components in ruminants. In particular aspects, the disclosure provides methods of increasing desirable components of milk in ruminants. Furthermore, the disclosure provides for methods of modulating the rumen microbiome.

Abstract: Compositions and methods for soil and groundwater remediation are contemplated as comprising a zero valent metal having a metal sulfide surface layer and an organic compound operative to degrade to produce molecular hydrogen. Such compositions may also include particulate activate carbon and anaerobic bacteria operative to perform reductive dechlorination. These compositions synergistically operate to remove chlorinated hydrocarbons from contaminated soil and groundwater.

Abstract: Compositions of insulin-secreting cells and stem cells and methods for their use as well as use of insulin and stem cell products in wound healing and/or reducing scar and/or scab formation are provided.

Abstract: The present application relates to a Lactobacillus paracasei ET-22 strain and use thereof. The present application provides a Lactobacillus paracasei ET-22 strain with a deposit number of CGMCC No. 15077, a composition containing the strain and a method of treating a subject using the strain. The present application also relates to a method for treating a subject, including administering an effective amount of the Lactobacillus paracasei ET-22 strain to a subject in need thereof for 1) whitening teeth and/or inhibiting oral pathogens; 2) adjusting balance of flora in the subject; 3) promoting growth of bifidobacteria and/or lactic acid bacteria; and/or 4) enhancing immunity of the subject.

Abstract: The present invention provides a production method of a cell aggregate containing an anterior eye segment tissue or a partial structure thereof, or a precursor tissue thereof, including culturing pluripotent stem cell aggregates in suspension in the presence of a bone morphogenic factor signal transduction pathway activating substance to induce self-organization of an anterior eye segment tissue or a partial structure thereof, or a precursor tissue thereof. In one embodiment, the bone morphogenic factor signal transduction pathway activating substance is BMP4. In one embodiment, the suspension culture is performed entirely or partially in the presence of a fibroblast growth factor. The produced cell aggregate can further contain a neural retinal tissue.

Abstract: Devices, methods, kits and means for easy, accurate, and fast estimation of soil microbial load, including fungal load, and fungal to bacterial ratio are described. The methods include extracting soil biomass into an extraction fluid to obtain a soil extract and detecting the fungal fraction, the bacterial fraction, and the fungal to bacterial ratio in the soil extract. The devices typically include microBIOMETER®, devices for particle size discrimination, and devices for absorbance and reflectance detection. The difference in microscopic sizes or spectroscopic absorbance of the fungal particles relative to those of the bacterial particles is used to compute the contribution of each type of particle to the biomass. In one example, the relative coverage of the microBIOMETER® device membrane by the fungal and/or bacterial particles can be measured by and then calculated using a cell phone camera and application.

Abstract: Phenotypic antimicrobial susceptibility testing (AST), the gold-standard diagnostic that indicates whether an antimicrobial will be clinically effective, often suffer the slowest times-to-result for the most resistant pathogens. Here we introduce novel assays to be performed in parallel with standard AST assays that enable rapid, same-shift reporting of AST results for a plurality of pathogens. The assays developed here are further capable of detecting resistance to carbapenems, the most powerful class of beta-lactams commonly used as “last-resort” antimicrobials.

Abstract: A method of producing cellulase includes steps (1) to (3): (1) subjecting an aqueous solution of cellulase derived from filamentous fungi to filtration through an ultrafiltration membrane with a molecular weight cut off of 100,000 to 200,000 to obtain a filtrate and concurrently obtain a concentrated enzyme liquid as a retentate; (2) further subjecting the filtrate obtained in step (1) to filtration through a second ultrafiltration membrane with a molecular weight cut off of 5,000 to 50,000 to obtain a second concentrated enzyme liquid as a retentate; and (3) mixing the concentrated enzyme liquid obtained in steps (1) and (2) to obtain cellulase derived from filamentous fungi.

Abstract: Disclosed herein are compositions and methods for diagnosing fungal dysbiosis in the gut of a subject with cirrhosis that involves assaying a sample from the subject for bacterial taxa. These compositions and methods are based on the discovery that there is significant correlation between fungal and bacterial diversity in gut microbiota of cirrhotic patients. Therefore, disclosed herein is a method for treating a subject with liver cirrhosis that involves assaying a gut sample from the subject for bacterial taxa to generate a bacterial profile, comparing the bacterial profile to control profiles to predict fungal dysbiosis; and treating the subject for fungal dysbiosis.

Abstract: Disclosed is a method for evaluating the ability of a chemical substance or a chemical composition to prevent muscle fatigue and damage induced by physical exertion in humans; edible composition including at least one salt of a multivalent metal cation, at least one compound selected between vitamin E or vitamin E acetate, at least one edible polyphenol compound selected from compounds of the flavonol family, compounds of the anthocyanin family, compounds of the phenolic acid family and compounds of the flavonol family and/or the glucosylated derivatives thereof, in which the molar ratio/is of at least 0.50 and not more than 2.00; its use as a food supplement or for preparing a food supplement composition or as a drug for preventing muscle fatigue and/or muscle damage induced by physical exertion, in a method for the treatment of the human body by therapy.

Abstract: Provided herein are methods and compositions related to Blautia Strain A useful as therapeutic agents.

Abstract: The disclosure relates to methods, systems and compositions for use in the production of milk. More specifically, the disclosure is directed to systems, compositions and methods for in-vitro production of milk using an array of mammary organoids seeded on tertiary-branched, resilient duct scaffolding.

Abstract: Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.

Abstract: A Kurthia gibsonii strain EO-06 with Deposit Number of CGMCC No. 18436, a Clostridium kogasensis strain EO-08 with Deposit Number of CGMCC No. 18438 and a Clostridium acidisoli strain EO-09 with Deposit Number of CGMCC No. 18439 are provided. The above strains can be used to treat pollution, for example, to treat industrial gas or wastewater containing ethylene oxide, which greatly improves the decontamination disposal capacity of ethylene oxide in industrial production. The present disclosure also provides a degradation agent for degrading ethylene oxide and a method for biodegrading ethylene oxide.

Abstract: Provided herein are methods and compositions relating, in part, to the generation of human progenitor cells committed to the lung lineage and uses of such cells for treatment of lung diseases/disorders or injury to the lung. Whether an adult stem cell can be isolated from human adult lung remains controversial in the art and at present, methods for isolating and using adult lung stem cells from humans lack reproducibility. Thus, the methods and compositions described herein are advantageous over the present state of knowledge in the art and permit the generation of human lung progenitor cells for treatment, tissue engineering, and screening assays.

Abstract: The present invention provides tools and methods for degrading MTBE, TBA and/or HCHO using abacierial consortium comprising one or more strains selected from Methylibium LD3, Hydrogenophaga LD1 and/or Mycobacterium LD6.

Abstract: Provided herein are pharmaceutical compositions comprising tumoricidal and/or antimicrobial components isolated from the supernatant of NK-92 cell medium and methods of using the compositions for killing cancer cells.

Abstract: Systems and methods of making lactobionic acid are described. The systems include two-compartment cation bipolar electrodialysis assemblies having at least one cell that includes a cation ion-exchange membrane and a bipolar membrane. The membranes define the borders of a pair of flow channels for a separate (i) caustic stream and (i) purified lactobionic acid stream. Lactobionate ions in the lactobionic acid stream do not cross a membrane in the electrodialysis assembly, which reduces membrane fouling. The methods include passing a lactobionate salt through a two-compartment cation bipolar electrodialysis assembly. The electrodialysis assembly includes at least one two-compartment cation bipolar membrane cell, and separates the lactobionate salt into a caustic compound and the lactobionic acid. The assembly is designed so the lactobionate ions do not cross an ion exchange membrane in the assembly to form the lactobionic acid, which reduces membrane fouling.